ABSTRACT
The herb Isodon serra (Maximowicz) Kudô, which is widely distributed in China and its neighbor regions, is a well-known traditional Chinese medicinal plant. In this study, we characterized the complete plastid genome sequence of I. serra using Illumina sequencing data. The plastome is 152,676 bp in length and contains a typical quadripartite structure. The inverted repeat (IR), large-single copy (LSC) and small-single copy (SSC) regions each has 25,716 bp, 83,564 bp, and 17,680 bp. The genome contains 80 protein coding genes (PCGs), 30 transfer RNAs (tRNA), and four ribosomal RNAs (rRNA). The phylogenetic result indicates I. serra together with genera Ocimum and Lavandula formed tribe Ocimeae clade.
ABSTRACT
Isodon lophanthoides var. graciliflorus is a folk medicinal herb that is distributed in tropical and subtropical Asia. In this study, the complete plastome of I. lophanthoides var. graciliflorus was assembled and annotated. The plastome is 152,195 bp in length, consisting of a large single-copy (LSC) region of 83,095 bp, a small single-copy (SSC) region of 17,699 bp, and two inverted repeat (IR) regions of 25,701 bp, each. It has 113 genes, including 80 protein-coding genes, 29 tRNA genes, and 4 rRNA genes. The overall GC content of the plastome is 37.6%. Phylogenetic analysis showed that I. lophanthoides var. graciliflorus was sister to Isodon serra.
ABSTRACT
OBJECTIVE: To establish an analytical method for characteristic fingerprint and determination of main components of Andrographis paniculata Extract by UPLC. METHODS: The chromatographic conditions were Waters ACQUITY UPLC BEH-C18 (2. 1 mm x 0 mm,1.7 µm)by gradient elute using acetonitrile-water as mobile phase(0 -2 min,20% ~ 25% A;2 ~ 5 min,25% ~ 35% A;5 ~ 7 min,35% A;7 ~10 min,35%~ 55% A) at a flow rate of 0. 5 min/mL,detecting wavelength at 220 nm. Results:Contents of the andrographolide, neoandrographolide, 14-deoxyandrographolide and 14-deoxy-l11,12-didehydroandrographolide had good resolution with the correlation coefficients exceed 0. 9999 and the average percent recovery lied in 97. 2% to 103.9%, RSD was less than 3.0% (n = 6). The chromatograms of Andrographis paniculata Extract shared seven common peaks in which four of them were recognized by reference standard with the similarities over 0. 9. CONCLUSION: It is a fast,accurate and validated method,and can be useful in quality evaluations of Andrographis paniculata Extract.
