Chemical components and against alzheimer's disease effects of the calyxes of Physalis alkekengi L. var. franchetii (Mast.) Makino.

Physalis alkekengi L. var. franchetii (Mast.) Makino (PA), a traditional Chinese medicine, is utilised for treating dermatitis, sore throat, dysuria, and cough. This research aimed to identify the main constituents in the four extracted portions from the calyces of PA (PAC) utilising ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UPLC-Q-TOF-MS). The Alzheimer's disease (AD) mice model was induced by D-galactose (D-gal) combined with aluminium chloride (AlCl3). Subsequent investigation into the underlying mechanisms involved behavioural and histopathological observations. The results demonstrated that four extracted portions of PAC (PACE) significantly enhanced memory and learning abilities in the Morris water maze. The concentrations of Aß, tau and p-tau in brain tissue exhibited a significant decrease relative to the model group. Moreover, the four PACE treatment groups increased the glutathione (GSH) and superoxide dismutase (SOD) levels, while concurrently reducing malondialdehyde (MDA), interleukin-1ß (IL-1ß) and interleukin-6 (IL-6), tumour necrosis factor-α (TNF-α) levels. In summary, the current study demonstrates that the four PACE formulations exhibit beneficial anti-AD properties, with the most pronounced efficacy observed in the EA group. Additionally, PAC shows potential in mitigating neuroinflammation and oxidative damage by inhibiting the TLR4/NF-κB signalling pathway. This research lays a theoretical groundwork for the future clinical development and utilisation of PAC in treating AD.

Efficacy and safety of XELOX combined with anlotinib and penpulimab vs XELOX as an adjuvant therapy for ctDNA-positive gastric and gastroesophageal junction adenocarcinoma: a protocol for a randomized, controlled, multicenter phase II clinical trial (EXPLORING study).

Background: The efficacy of current adjuvant chemotherapy for gastric adenocarcinoma/gastroesophageal junction adenocarcinoma (GA/GEJA) leaves much to be desired. ctDNA could serve as a potential marker to identify patients who are at higher risk of recurrence. Reinforcing standard adjuvant chemotherapy with immunotherapy has already been indicated to significantly improve clinical outcome, albeit such evidence is rare in GA/GEJA. Here, we intend to explore the clinical benefit of the reinforcement of adjuvant immunotherapy and antiangiogenics alongside with chemotherapy in patients who are deemed in high risk of recurrence by ctDNA analysis, which might shed light on further improvements in adjuvant therapy for GA/GEJA. Methods/Design: This study is designed as a prospective, multicenter, randomized, controlled phase II study in patients histologically or cytologically diagnosed with GA/GEJA who underwent D2 gastrectomy and achieved R0 or R1 resection. From February 2022, a total of 300 stage III patients will be enrolled and subjected according to ctDNA sequencing results, and those with positive results will subsequently be randomized 1:1 to arm A or B. Patients in arm A will receive anlotinib, penpulimab and XELOX for 6-8 cycles, maintained with anlotinib and penpulimab for up to 1 year, while patients in arm B will receive XELOX alone for 6-8 cycles. ctDNA-negative patients will be assigned to arm C, and patients who are ctDNA positive but failed in randomization will be assigned to arm D. Patients in arms C and D will receive the investigator's choice of therapy. The primary endpoint is the median disease-free survival (DFS) of arm A versus arm B determined via CT/MRI imaging. Secondary endpoints include the DFS of ctDNA positive patients versus ctDNA negative patients, the 2- and 3-year DFS rates, overall survival (OS), the impact of hallmark molecules on the treatment response, adverse events (AEs), and the impact of nutrition status or exercise on recurrence. Discussion: We expect that ctDNA would be a strong prognostic factor and ctDNA-positive patients are at higher risk of relapse than ctDNA-negative patients. The addition of anlotinib and penpulimab to XELOX, may contribute to delaying relapse in ctDNA-positive patients. Trial registration: https://www.clinicaltrials.gov, identifier NCT05494060.

Unraveling the behaviors of sulfonamide antibiotics on the production of short-chain fatty acids by anaerobic fermentation from waste activated sludge and the microbial ecological mechanism.

Antibiotics in waste activated sludge (WAS) has drawn increasing attention because of their persistent and bioaccumulation characteristics. Most study illustrated the role of antibiotics in anaerobic fermentation from WAS, but lacking the analysis at microbial level as well as the possible interaction between them. This study investigated the effect of three sulfonamide antibiotics (sulfamethoxazole (SMX), sulfaquinoxaline (SQX), and sulfadiazine (SD)) on WAS fermentation and explored its microbiological mechanism. Results indicated that the production of short-chain fatty acids (SCFAs) was significantly improved by 1.9 folds with a peak value at 4626.1 mg COD L-1 in the existence of SD. This was attributed to the promoted release of soluble proteins and polysaccharides with the existence of sulfonamide antibiotics (SAs) as revealed by the excitation-emission matrix (EEM) spectrum. Analysis of microbial community structure showed that the total abundance of the fermenters in groups with SAs was1.2-1.6 times of that in Control. Specifically, the acid-forming genus Tissierella in SMX and SQX increased by 12.1%-15.0% compared with the Control, while the proteolytic genus Proteinivorax dominated in SD with 39.5%. Molecular ecological networks (MENs) analysis further revealed the potential cooperative relationships among different fermenters. This study was anticipated to provide some valuable information for the behavior of antibiotics in WAS fermentation.

Elucidating the microbial ecological mechanisms on the electro-fermentation of caproate production from acetate via ethanol-driven chain elongation.

Electro-fermentation (EF) is an attractive way to implement the chain elongation (CE) process, by controlling the fermentation environment and reducing the dosage of external electron donors (EDs). However, besides the coexistence performance of external EDs and electrode, applications of EF technology on the fermentation broth containing both EDs and electron acceptors during CE process, are all still limited. The current study investigated the contribution of EF to caproate production, under different acetate: ethanol ratios (RA/E). The effect of multiple EDs, both from ethanol and the bio-cathode, on caproate production, was also assessed. A proof-of-concept, based on experimental data, was presented for the EF-mediated ethanol-driven CE process. Experimental results showed that ethanol, together with the additional electron donors from the bio-cathode, was beneficial for the stable caproate production. The caproate concentration increased with the decrease of RA/E, while the bio-cathode further contributed to 10.7%-26.1 % increase of caproate concentration. Meanwhile, the hydrogen partial pressure tended to 0.10 ± 0.01 bar in all controlled EF reactors, thus favoring caproate production. This was attributed to the increased availability EDs, i.e., hydrogen and ethanol, generated by the electrode and electrochemically active bacteria (EAB), which might create multiple additional pathways to achieve caproate production. Molecular ecological networks analysis of the key microbiomes further revealed underlying cooperative relationships, beneficial to the chain elongation process. The genus Clostridium_sensu_stricto, as the dominant microbial community, was positively related to acetogens, EAB and fermenters.

Low-dose apatinib combined with camrelizumab and the SOX regimen in the neoadjuvant treatment of locally advanced gastric/gastroesophageal junction adenocarcinoma (SPACE-neo): a protocol for an open-label, single-arm, clinical trial.

Background: Neoadjuvant chemotherapy with S-1 plus oxaliplatin (SOX regimen) has shown promising results in pathological response rate and survival rate in patients with locally advanced resectable gastric cancer (LAGC). We previously carried out the SPACE study to assess efficacy and safety of low-dose apatinib combined with camrelizumab and the SOX regimen as a first-line treatment of advanced gastric/gastroesophageal junction adenocarcinoma (AGC/GEJC). The preliminary results demonstrated a high objective response rate. However, the SPACE study was conducted in patients with AGC, but the efficacy of LAGC patients is not yet known. The SPACE-neo study is designed to investigate whether this combination could improve outcomes in patients with locally advanced gastric/gastroesophageal junction cancer (LAGC/GEJC) as neoadjuvant therapy. Methods: SPACE-neo is a prospective, open-label, single-arm study conducted in China at the First Affiliated Hospital of Nanjing Medical University (Jiangsu Province Hospital). Thirty-two patients with human epidermal growth factor receptor 2 (HER2)-negative or HER2-unknown LAGC/GEJC confirmed by histopathology or cytology will be recruited. Included patients shall be clinically staged as M0 and either T3 to T4 or N+ assessed by ultrasound endoscopy and thoracoabdominal-enhanced computed tomography or magnetic resonance imaging. The patients will receive three cycles of this combined regimen as a neoadjuvant treatment. Each patient will receive screening visits within 2 weeks before the first cycle and planned visits before every cycle of treatment. Key monitoring data include imaging data, pathological findings, and adverse events associated with neoadjuvant and surgical treatment. The primary endpoints are major pathological response (MPR) and safety. MPR is the proportion of patients whose residual tumor cells make up less than 10% of the primary tumor from among the total cohort. Clopper-Pearson method will be used to estimate the 95% confidence interval of MPR and safety data will be reported as descriptive statistical analysis. Discussion: The SPACE-neo trial aims to evaluate the safety and preliminary efficacy of this regimen in the neoadjuvant treatment of LAGC/GEJC. It is hoped that the study can achieve a higher pathological response rate and longer survival rate. Trial Registration: ChiCTR.gov.cn: ChiCTR2100049305.

Initial-alkaline motivated fermentation of fine-sieving fractions and its effect on properties of cellulosic components.

Exploration of value-added products from wastewater treatment plants (WWTPs) was promising for its sustainable development. This study simultaneously addressed the possibility of volatile fatty acids (VFAs) production boost and cellulosic components recovery from fine-sieving fractions (FSF) under initial alkaline conditions. The step utilization of FSF was relatively untapped in similar literatures. The effect of different initial pH values with 8.5, 9.5 and 10.5 (defined as F-8.5, F-9.5 and F-10.5) on fermentation performance were investigated. Then, the fermentation residues were collected to evaluate the changes in chemical structure and thermodynamic properties by fourier transform infrared spectroscopy (FTIR) and thermo-gravimetric (TG) analysis. Furthermore, analysis of the changes in microbial community structure and the interaction between functional genus and performance parameters were undertaken by high throughput sequencing and canonical correspondence analysis (CCA). Results showed that F-10.5 obtained the highest VFAs yields of 234 mg/g VSS, due to efficient polysaccharides release and inhibited methane production. However, high alkaline intensity caused proteins denaturation. Acidogenesis kinetics suggested that the fermentation rate was chemical-dominated. Although crystalline structure was more disordered with increasing alkalinity, the weight loss was lower than 2.5%, making it possible to recover cellulose from fermented residues. Interaction between functional genus and performance parameters revealed the microbial mechanism during the alkaline fermentation. Consequently, the initial-alkaline motivated fermentation was proved to be a promising technology in value-added products recovery to be cost economic, energy positive and environmental friendly.

Downstream processing of lignin derived feedstock into end products.

Despite the enormous research efforts in recent years regarding lignin depolymerisation and functionalisation, few commercial products are available. This review provides a summary and viewpoint of extensive research in the lignin-to-product valorisation chain, with an emphasis on downstream processing of lignin derived feedstock into end products. It starts with an introduction of available platform chemicals and polymeric derivatives generated from lignin via existing depolymerisation and functionalisation technologies. Following that, detailed analyses of various strategies for the downstream processing of lignin derived platform chemicals and materials into fuels, valued-added chemicals and functional polymers are provided. A concise techno-economic analysis of various downstream processes is conducted based on the market demand of the end product, economic potential and technological readiness, enabling the identification of processes that are potentially both economically competitive and commercially feasible, and shedding light on processes which deserve further technological development. We wish this review will stimulate further advances in the sustainable production of value-added products from lignin to integrate this invaluable "bio-waste" into the chemical/materials supply chain.

Production of Terephthalic Acid from Corn Stover Lignin.

Funneling and functionalization of a mixture of lignin-derived monomers into a single high-value chemical is fascinating. Reported herein is a three-step strategy for the production of terephthalic acid (TPA) from lignin-derived monomer mixtures, in which redundant, non-uniform substitutes such as methoxy groups are removed and the desired carboxy groups are introduced. This strategy begins with the hydro-treatment of corn-stover-derived lignin oil over a supported molybdenum catalyst to selectively remove methoxy groups. The generated 4-alkylphenols are converted into 4-alkylbenzoic acids by carbonylation with carbon monoxide. The Co-Mn-Br catalyst then oxidizes various alkyl chains into carboxy groups, transforming the 4-alkylbenzoic acid mixture into a single product: TPA. For this route, the overall yields of TPA based on lignin content of corn stover could reach 15.5 wt %, and importantly, TPA with greater than 99 % purity was obtained simply by first decanting the reaction mixture and then washing the solid product with water.

Enhanced quinoline removal by zero-valent iron-coupled novel anaerobic processes: performance and underlying function analysis.

Quinoline is toxic and difficult to degrade biologically; thus, it is a serious threat to the safety of ecosystems. To promote quinoline reduction, zero-valent iron (ZVI) was introduced into an anaerobic digestion (AD) system through batch experiments. The performance of three different types of ZVI (i.e., iron powder, iron scrap and rusty iron scrap) on quinoline degradation, methane production, formation of volatile fatty acids (VFAs) and chemical oxygen demand (COD) removal were investigated systematically. Compared to the AD system alone, quinoline and COD removal as well as the production of methane and acetic acid were effectively enhanced by ZVI, especially rusty iron scrap. The removal efficiencies of quinoline and COD were increased by 28.6% and 19.9%, respectively. The enhanced effects were attributed to the high accumulation of ferrous ions and high pH self-buffering capability, which were established by ZVI addition. Furthermore, high-throughput sequencing analysis indicated that the functional microorganisms in the ZVI-AD system were higher than in the AD system, and the added types of ZVI played important roles in structuring the innate microbial community in waste activated sludge (WAS). Especially, high enrichment of microorganisms capable of degrading quinoline, such as Pseudomonas and Bacillus, in the coupled system was detected.

Efficient cleavage of aryl ether C-O linkages by Rh-Ni and Ru-Ni nanoscale catalysts operating in water.

Bimetallic Ru-Ni and Rh-Ni nanocatalysts coated with a phase transfer agent efficiently cleave aryl ether C-O linkages in water in the presence of hydrogen. For dimeric substrates with weaker C-O linkages, i.e. α-O-4 and ß-O-4 bonds, low loadings of the precious metal (Rh or Ru) in the nanocatalysts quantitatively afford monomers, whereas for the stronger 4-O-5 linkage higher amounts of the precious metal are required to achieve complete conversion. Under the optimized, relatively mild operating conditions, the C-O bonds in a range of substituted ether compounds are efficiently cleaved, and mechanistic insights into the reaction pathways are provided. This work paves the way to sustainable approaches for the hydrogenolysis of C-O bonds.

Harnessing the Wisdom in Colloidal Chemistry to Make Stable Single-Atom Catalysts.

Research on single-atom catalysts (SACs), or atomically dispersed catalysts, has been quickly gaining momentum over the past few years. Although the unique electronic structure of singly dispersed atoms enables uncommon-sometimes exceptional-activities and selectivities for various catalytic applications, developing reliable and general procedures for preparing stable, active SACs in particular for applications under reductive conditions remains a major issue. Herein, the challenges associated with the synthesis of SACs are highlighted semiquantitatively and three stabilization techniques inspired by colloidal science including steric, ligand, and electrostatic stabilization are proposed. Some recent examples are discussed in detail to showcase the power of these strategies in the synthesis of stable SACs without compromising catalytic activity. The substantial further potential of steric, ligand, and electrostatic effects for developing SACs is emphasized. A perspective is given to point out opportunities and remaining obstacles, with special attention given to electrostatic stabilization where little is done so far. The stabilization strategies presented herein have a wide applicability in the synthesis of a series of new SACs with improved performances.

Microbial community response reveals underlying mechanism of industrial-scale manganese sand biofilters used for the simultaneous removal of iron, manganese and ammonia from groundwater.

Most studies have employed aeration-biofiltration process for the simultaneous removal of iron, manganese and ammonia in groundwater. However, what's inside the "black box", i.e., the potential contribution of functional microorganisms behavior and interactions have seldom been investigated. Moreover, little attention has been paid to the correlations between environmental variables and functional microorganisms. In this study, the performance of industrial-scale biofilters for the contaminated groundwater treatment was studied. The effluent were all far below the permitted concentration level in the current drinking water standard. Pyrosequencing illustrated that shifts in microbial community structure were observed in the microbial samples from different depths of filter. Microbial networks showed that the microbial community structure in the middle- and deep-layer samples was similar, in which a wide range of manganese-oxidizing bacteria was identified. By contrast, canonical correlation analysis showed that the bacteria capable of ammonia-oxidizing and nitrification was enriched in the upper-layer, i.e., Propionibacterium, Nitrosomonas, Nitrosomonas and Candidatus Nitrotoga. The stable biofilm on the biofilter media, created by certain microorganisms from the groundwater microflora, played a crucial role in the simultaneous removal of the three pollutants.

Publisher Correction: Microbial network of the carbonate precipitation process induced by microbial consortia and the potential application to crack healing in concrete.

A correction to this article has been published and is linked from the HTML version of this paper. The error has been fixed in the paper.

Microbial network of the carbonate precipitation process induced by microbial consortia and the potential application to crack healing in concrete.

Current studies have employed various pure-cultures for improving concrete durability based on microbially induced carbonate precipitation (MICP). However, there have been very few reports concerned with microbial consortia, which could perform more complex tasks and be more robust in their resistance to environmental fluctuations. In this study, we constructed three microbial consortia that are capable of MICP under aerobic (AE), anaerobic (AN) and facultative anaerobic (FA) conditions. The results showed that AE consortia showed more positive effects on inorganic carbon conversion than AN and FA consortia. Pyrosequencing analysis showed that clear distinctions appeared in the community structure between different microbial consortia systems. Further investigation on microbial community networks revealed that the species in the three microbial consortia built thorough energetic and metabolic interaction networks regarding MICP, nitrate-reduction, bacterial endospores and fermentation communities. Crack-healing experiments showed that the selected cracks of the three consortia-based concrete specimens were almost completely healed in 28 days, which was consistent with the studies using pure cultures. Although the economic advantage might not be clear yet, this study highlights the potential implementation of microbial consortia on crack healing in concrete.

Thermally stable single atom Pt/m-Al2O3 for selective hydrogenation and CO oxidation.

Single-atom metal catalysts offer a promising way to utilize precious noble metal elements more effectively, provided that they are catalytically active and sufficiently stable. Herein, we report a synthetic strategy for Pt single-atom catalysts with outstanding stability in several reactions under demanding conditions. The Pt atoms are firmly anchored in the internal surface of mesoporous Al2O3, likely stabilized by coordinatively unsaturated pentahedral Al3+ centres. The catalyst keeps its structural integrity and excellent performance for the selective hydrogenation of 1,3-butadiene after exposure to a reductive atmosphere at 200 °C for 24 h. Compared to commercial Pt nanoparticle catalyst on Al2O3 and control samples, this system exhibits significantly enhanced stability and performance for n-hexane hydro-reforming at 550 °C for 48 h, although agglomeration of Pt single-atoms into clusters is observed after reaction. In CO oxidation, the Pt single-atom identity was fully maintained after 60 cycles between 100 and 400 °C over a one-month period.

Characterization of biocarbon-source recovery and microbial community shifts from waste activated sludge by conditioning with cornstover: Assessment of cellulosic compositions.

Most studies on the production of volatile fatty acids (VFAs) from waste activated sludge (WAS) digestion have focused on operating conditions, pretreatments and characteristic adjustments. Conditioning by extra carbon sources (ECS), normally added in a solid form, has been reported to be an efficient approach. However, this has caused considerable waste of monomeric sugars in the hydrolysate. In this study, the effects of two added forms (pretreated straw (S) and hydrolyzed liquid (L)) of cornstover (CS) on WAS acidification were investigated. To obtain different cellulosic compositions of CS, low-thermal or autoclaved assisted alkaline (TA or AA) pretreatments were conducted. The results showed that AA-L test achieved the highest VFAs value (653 mg COD/g VSS), followed by AA-S (613 mg COD/g VSS). These values were 12% and 28% higher, respectively, than that obtained in the TA-L and TA-S tests. Meanwhile, higher percentages of acetic acid were observed after AA pretreatment (~62% versus ~53% in TA). The added forms of CS played an important role in structuring the innate microbial community in the WAS, as shown by high-throughput sequencing and canonical correspondence analysis. The findings obtained in this work may provide a scientific basis for the potential implementation of co-digesting WAS with ECS simultaneously obtaining energy and high value-added products.

What could the entire cornstover contribute to the enhancement of waste activated sludge acidification? Performance assessment and microbial community analysis.

BACKGROUND: Volatile fatty acids (VFAs) production from waste activated sludge (WAS) digestion is constrained by unbalanced nutrient composition (low carbon-to-nitrogen ratio). Characteristics conditioning by extra carbon sources, normally in the mixture of raw solid, has been reported to be an efficient approach to enhance WAS acidification. However, little attention has been paid to the contributions of other adjustment forms. Moreover, the corresponding ecological estimation has not been investigated yet. RESULTS: In this study, the feasibility of corn stover (CS) conditioning with three adjustment forms [pretreated straw (S), hydrolysate (H) and hydrolysate + straw (HS)] in improving VFAs production from WAS was demonstrated. It was observed that the highest VFAs yield was achieved in H co-digesting test (574 mg COD/g VSS), while it was only 392 mg COD/g VSS for WAS digesting alone. VFAs composition was strongly adjustment form-dependent, as more acetic (HAc) and propionic (HPr) acids were generated in CS_HS and S, respectively. High-throughput sequencing analysis illustrated that acid (especially HAc)-producing characteristic genera (Bacteroides, Proteiniclasticum and Fluviicola) and HPr-producing characteristic genera (Mangroviflexus and Paludibacter) were detected by CS_HS and S conditioning, respectively. CONCLUSIONS: Corn stover conditioning greatly upgraded the WAS acidification performance, especially for the CS_H adjustment form, and the VFAs yield gained was considerably larger than that previously reported. CS adjustment forms played an important role in structuring the innate microbial community in WAS. Canonical correlation analysis illustrated that characteristic genera, with better hydrolysis and acidification abilities, could be enriched by the feedstocks with certain content of cellulose, hemicellulose or their saccharification hydrolysates. Moreover, ecological estimation revealed that, as far as the entire CS (including S and H) per acre was concerned, the capacity of WAS treatment would reach that produced in a one million mts capacity wastewater treatment plants (WWTPs) per day. These findings may have crucial implications for the operation of WWTPs.

Direct Conversion of Mono- and Polysaccharides into 5-Hydroxymethylfurfural Using Ionic-Liquid Mixtures.

Platform chemicals are usually derived from petrochemical feedstocks. A sustainable alternative commences with lignocellulosic biomass, a renewable feedstock, but one that is highly challenging to process. Ionic liquids (ILs) are able to solubilize biomass and, in the presence of catalysts, convert the biomass into useful platform chemicals. Herein, we demonstrate that mixtures of ILs are powerful systems for the selective catalytic transformation of cellulose into 5-hydroxymethylfurfural (HMF). Combining ILs with continuous HMF extraction into methyl-isobutyl ketone or 1,2-dimethoxyethane, which form a biphase with the IL mixture, allows the online separation of HMF in high yield. This one-step process is operated under relatively mild conditions and represents a significant step forward towards sustainable HMF production.

Stabilizing a Platinum1 Single-Atom Catalyst on Supported Phosphomolybdic Acid without Compromising Hydrogenation Activity.

In coordination chemistry, catalytically active metal complexes in a zero- or low-valent state often adopt four-coordinate square-planar or tetrahedral geometry. By applying this principle, we have developed a stable Pt1 single-atom catalyst with a high Pt loading (close to 1 wt %) on phosphomolybdic acid(PMA)-modified active carbon. This was achieved by anchoring Pt on the four-fold hollow sites on PMA. Each Pt atom is stabilized by four oxygen atoms in a distorted square-planar geometry, with Pt slightly protruding from the oxygen planar surface. Pt is positively charged, absorbs hydrogen easily, and exhibits excellent performance in the hydrogenation of nitrobenzene and cyclohexanone. It is likely that the system described here can be extended to a number of stable SACs with superior catalytic activities.