The Impact of Artificial Restoration of Alpine Grasslands in the Qilian Mountains on Vegetation, Soil Bacteria, and Soil Fungal Community Diversity.

To understand how the soil microbial community structure responds to vegetation restoration in alpine mining areas, this study specifically examines the grassland ecosystem in the Qianmalong mining area of the Qilian Mountains after five years of artificial restoration. High-throughput sequencing methods were employed to analyze soil bacteria and fungi microbial characteristics in diverse grassland communities. Combined with modifications in vegetation diversity as well as soil physicochemical properties, the impact of vegetation restoration on soil microbiome diversity in this alpine mining area was investigated. The findings indicated that the dominant plants were Cyperus rotundus, Carex spp., and Elymus nutans. As the extent of the grassland's restoration increased, the number of plant species, importance values, and plant community diversity showed an increasing trend. The plant functional groups were mainly dominated by Cyperaceae, followed by Poaceae. Plant height, density, plant cover, frequency, and aboveground biomass showed an increasing trend, and soil water content (SWC) increased. While soil pH and soil electrical conductivity (EC) exhibited a declining trend, available phosphorus (AP), total phosphorus (TP), total nitrogen (TN), nitrate nitrogen (NO3-N), soil organic carbon (SOC), and soil water content (SWC) showed an increasing trend. The dominant bacterial communities were Actinobacteriota, Proteobacteria, Acidobacteriota, Chloroflexi, Firmicutes, and Gemmatimonadota, while the dominant fungal communities were Ascomycota, Mortierellomycota, Basidiomycota, unclassified_k_Fungi, and Glomeromycota. Significant differences were detected within soil microbial community composition among different degrees of restoration grasslands, with bacteria generally dominating over fungi. SWC, TP, and TN were found to be the main soil physicochemical factors affecting the distribution of soil bacterial communities' structure; however, SOC, TN, and NO3-N were the primary factors influencing the soil distribution of fungal communities. The results of this study indicate that different degrees of vegetation restoration in alpine mining areas can significantly affect soil bacterial and fungal communities, and the degree of restoration has varying effects on the soil bacteria and fungi community structure in alpine mining areas.

Vegetation communities and soil properties along the restoration process of the Jinqianghe mine site in the Qilian Mountains, China.

The study explores the impact of mine grassland restoration on plant communities and soil properties in alpine grasslands, a subject of significant interest due to the observed relationship between grassland changes, plant communities, and soil properties. While prior research has mainly focused on the consequences of grassland degradation on plant diversity and soil characteristics, the specific effects of varying restoration degrees in alpine mining grasslands at the regional scale remain poorly understood. To address this knowledge gap, we established 15 sampling plots (0.5m×0.5m) across five different restoration degrees within alpine mining grasslands in the Qilian Mountains, China. Our objective was to assess the variations in plant diversity and soil properties along these restoration gradients. We conducted comprehensive analyses, encompassing soil properties [soil water content (SWC), available nitrogen (AN), total phosphorus (TP), nitrate nitrogen (NO3-N), ammonium nitrogen (NH4-N), total nitrogen (TN), available phosphorus (AP), soil organic carbon (SOC), nitrate nitrogen, soil pH, and electrical conductivity (EC)], plant characteristics (height, density, frequency, coverage, and aboveground biomass), and plant diversity indices (Simpson, Shannon-Wiener, Margalef, Dominance, and Evenness indexes). Our findings included the identification and collection of 18 plant species from 11 families and 16 genera across the five restoration degrees: Very Low Restoration Degree (VLRD), Low Restoration Degree (LRD), Moderate Restoration Degree (MRD), High Restoration Degree (HRD), and Natural Grassland (NGL). Notably, species like Carex duriuscula, Cyperus rotundus, and Polygonum viviparum showed signs of recovery. Principal component analysis and Pearson correlation analysis revealed that soil pH, SWC, SOC, NO3-N, and AN were the primary environmental factors influencing plant communities. Specifically, soil pH and EC decreased as restoration levels increased, while SWC, AN, TP, NH4-N, TN, AP, SOC, and NO3-N exhibited a gradual increase with greater restoration efforts. Furthermore, the HRD plant community demonstrated similarities to the NGL, indicating the most effective natural recovery. In conclusion, our study provides valuable insights into the responses of plant community characteristics, plant diversity, and soil properties across varying restoration degrees to environmental factors. It also elucidates the characteristics of plant communities along recovery gradients in alpine grasslands.

The role of land use change in affecting ecosystem services and the ecological security pattern of the Hexi Regions, Northwest China.

The land use and land cover change (LUCC) associated with climate change and human activities is supposed to exert a significant effect on ecosystem functions in arid inland regions. However, the role of LUCC in shaping the spatio-temporal patterns of ecosystem services and ecological security remain unclear, especially under different future LUCC scenarios. Here, we evaluated dynamic changes of ecosystem services and ecological security pattern (ESP) in the Hexi Regions based on LUCC and other environment variables by integrating morphological spatial pattern analysis (MSPA), entropy weight method and circuit theory. Our result showed that the LUCC was generally stable from 1980 to 2050. Compare to 2020, the land conversion under natural growth (NG), ecological protection (EP) and urban development (UD) scenarios in 2050 has changed by 10.30 %, 10.10 %, and 10.31 %, respectively. The forest, medium-cover grassland and water increased in the EP scenario, and construction land and cropland greatly expanded in the other two scenarios. Ecosystem services grew larger in the EP scenario by 2050 in comparison with the NG and UD scenarios. The ESP in the Hexi Regions has obvious spatial differences during 1980-2050. The larger ecological sources and less resistance corridors were mainly distributed in the central and eastern of the Hexi Regions with high ecosystem services. Conversely, fragmented ecological sources and larger resistance corridors were mostly located in the western regions blocked by sandy land, bare land or mountains. Compared to 2020, the area of ecological sources and pinch points under the EP scenario in 2050 increased by 4.10 × 103 km2 and 0.31 × 103 km2, respectively. The number of ecological corridors reduced while the length and resistance increased apart from the EP scenario. Our results highlighted the importance of ecological protection in shaping the LUCC, which further enhances the integrity of ecosystem and ecological security.

Effects of land use and land cover change on soil organic carbon storage in the Hexi regions, Northwest China.

Soil organic carbon (SOC) storage in arid inland regions is significantly affected by land use and land cover change (LUCC) associated with climate change and agricultural activities. A systematic evaluation to the LUCC effects on SOC storage could enable us to better manage soil carbon pools in arid inland regions. Here, we evaluated the effects of LUCC on SOC storage in the Hexi Regions based on high-resolution SOC and LUCC maps derived from Landsat imagery and digital soil mapping using machine learning algorithm and environmental covariates. The results showed that SOC generally increased from northwest to southeast over the Hexi Regions with an average stock of 7.15 kg C m-2 at a soil depth of 100 cm and a total storage of 2783.05 Tg C. The SOC stock and storage in the Qilian Mountains (mountains) was about 3.90 and 4.55 times higher than that in the Hexi Corridor (plains), respectively. It was estimated that LUCC over the past four decades caused a net increase of 23.41 and 18.19 Tg C in total SOC storage for the Qilian Mountains and Hexi Corridor, respectively. Specifically, the development in grasslands quality as well as the land-use category conversion from the bare land to grassland mainly contributed to the increase in SOC storage of the Qilian Mountains, where the LUCC was mainly driven by climate change. By contrast, the SOC storage change in the Hexi Corridor was mainly associated with the conversion from sandy land and low-cover grassland to cropland as well as sandy land to grassland, being mainly affected by intense cropland expansion and desertification control. Our results highlighted the importance of climate change and cropland expansion in enhancing SOC storage of the Qilian Mountains and Hexi corridor, respectively.

Carrying capacity for vegetation across northern China drylands.

Revegetation and afforestation across drylands for establishing sustainable ecosystems requires a comprehensive understanding of the carrying capacity for vegetation (CCV) at the regional scale. To determine the CCV across drylands in northern China, we developed a technical framework based on two measures of leaf area index (LAI): maximum LAI (Max-LAI) and safe LAI (Safe-LAI), and their thresholds, CCVmax and CCVsafe, for six drylands (Horqin, Hulun Buir, Otindag, Mu Us, Tengger, and Junggar) using remote sensing datasets from 2000 to 2014. We also predicted dynamics of CCV of the drylands over the next decade (2015-2024) by establishing optimal prediction models based on environmental factors (temperature, precipitation, potential evapotranspiration, and elevation). According to these models, the Max-LAI threshold (range: 0.36-1.03 m2/m2) and Safe-LAI threshold (0.29-0.70 m2/m2) declined from east to west with decreases in aridity index. Under current climatic variability and anthropogenic disturbances, the CCV in most drylands would have positive increments (approximately 15%), except in the Horqin (approximately -15%) and Tengger (slight changes), during the following decade. This indicates that there is scope for improving vegetation coverage in most drylands, except in the Horqin and Tengger. Our results suggest that revegetation and ecosystem management to prevent ongoing desertification should be carried out at the regional scale. Although it does not account for biocrusts, artificially introduced vegetation, underground water, and other vegetation attributes (e.g., density and biomass), our technical framework and results might nonetheless be valuable in evaluating regional ecological security and guiding vegetation restoration of drylands across northern China.