ABSTRACT
This work is aimed at the investigation of the senescence associated genes differentially expressed in rat muscle by cDNA microarray. After total RNA isolation, mRNA purification and probe preparation, the gene expression patterns of rat muscle from the old group (30 months old) and the young group (3 months old) were compared by cDNA gene chips. In the muscle of old rats there were reproducibly 127 differentially expressed genes, among which some down-regulated genes were involved in energy metabolism and signal transduction, while some up-regulated genes were related to protein degradation and cell apoptosis. Meanwhile muscle fiber atrophy with mitochondria vacuolation was found in aging rat by electron microscope.
Subject(s)
Aging , Gene Expression Profiling , Muscle, Skeletal/metabolism , Animals , Muscle, Skeletal/growth & development , Oligonucleotide Array Sequence Analysis/methods , RNA, Messenger/genetics , RNA, Messenger/metabolism , Rats , Rats, Sprague-DawleyABSTRACT
AIM: To build a easy and reliable rat model of insulin resistance. METHODS: Changes concerning sugar metabolism were observed in experimental rats injected intraperitoneally by different doses of dexamethasone. RESULTS: The changes by dexamethasone in fasting blood insulin and insulin resistance prior to that of fasting blood glucose were dose and time dependent. CONCLUSION: It was convenience to set up a insulin resistance model induced by dexamethasone in rats which would favor other related research.
