IL23R as an indicator of immune infiltration and poor prognosis in intrahepatic cholangiocarcinoma: a bioinformatics analysis.

Background: Although the incidence of intrahepatic cholangiocarcinoma (CHOL) is low, the prognosis is very poor. The expression level of interleukin 23 receptor (IL23R) is linked to the occurrence and development of cancers. This study aimed to identify the role of IL23R in CHOL using bioinformatics tools and experimental validation. Methods: Circular RNA (circRNA), microRNA (miRNA), and messenger RNA (mRNA) datasets were obtained from the Gene Expression Omnibus (GEO) database, and R software was used for data analysis and visualization. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) were used to conduct functional enrichment analysis, which was verified with gene set enrichment analysis software. Clinical data were obtained from The Cancer Genome Atlas (TCGA), and survival analyses were performed using the DriverDBv3 database and the Gene Expression Profiling Interactive Analysis website. The TIMER2.0 database provided us for immune cell infiltration analysis results of IL23R. Real-time quantitative polymerase chain reaction (RT-qPCR) was used for IL23R expression verification. Results: Differentially expressed (DE) mRNAs were enriched in phosphoinositide 3-kinase-serine/threonine kinase signaling pathway, immune-related tumor microenvironment (TME), and amino acid metabolism, etc. In addition, expression of IL23R was associated with immune infiltration-related cells. Furthermore, a circRNA-miRNA-IL23R network and a IL23R protein-protein interaction network were established. Most importantly, IL23R, as a prognostic gene, was found to have a low expression in CHOL. Conclusions: A circRNA-miRNA-IL23R network was identified, and it was found that IL23R may be a prognostic and immune-related biomarker in CHOL, which is worthy of further exploration.

Childhood emotional but not physical or sexual maltreatment predicts prosocial behavior in late adolescence: A daily diary study.

BACKGROUND: Childhood maltreatment (CM), including physical, emotional, and sexual maltreatment, is detrimental to adolescents' psychological and behavioral outcomes. However, most studies on the relationship between CM and prosocial behavior focused on the overall experience of CM. Since different forms of CM exert various influences on adolescents, it is essential to find out which form of CM has the strongest link with prosocial behavior and the underlying mechanism behind it to fully understand this relationship and design a specific intervention for promoting prosocial behavior. OBJECTIVE: Guided by internal working model theory and hopelessness theory, this study aimed to investigate the connections of multiple forms of CM with prosocial behavior, and explore the mediating mechanism of gratitude from the perspective of the broaden-and-build theory through a 14-day daily diary study. PARTICIPANTS: A total of 240 Chinese late adolescents (217 females; Mage = 19.02, SDage = 1.83) from a college volunteered for this study and completed questionnaires regarding CM, gratitude, and prosocial behavior. METHODS: A multilevel regression analysis was conducted to investigate which form of CM was correlated to prosocial behavior, and a multilevel mediation analysis was applied to examine the underlying mechanism (i.e., gratitude) behind this relationship. RESULTS: The results of the multilevel regression analysis showed that it was childhood emotional maltreatment, but not physical or sexual maltreatment that negatively predicted prosocial behavior. The results of the multilevel mediation analysis indicated that gratitude mediated the relationship between childhood emotional maltreatment and prosocial behavior. CONCLUSIONS: Findings from the present study highlight the predictive effect of childhood emotional maltreatment on late adolescents' prosocial behavior and the mediating role of gratitude in this link.

Relation of emotion malleability beliefs to subjective well-being: A short-term longitudinal study.

Prior research has shown that emotion malleability beliefs are positively related to subjective well-being, but less is known about the longitudinal relationship between both variables. The present study used a two-wave longitudinal design to examine the temporal directionality of the relationship in a sample of Chinese adults. Using cross-lagged models, we found that emotion malleability beliefs predicted all three dimensions of subjective well-being (i.e. positive affect, life satisfaction, and negative affect) 2 months later. However, we did not detect any reverse or reciprocal effect between emotion malleability beliefs and subjective well-being. In addition, emotion malleability beliefs still predicted life satisfaction and positive affect after controlling for the effect of the cognitive or emotional component of subjective well-being. Our study provided primary evidence for the temporal directionality of the association between emotion malleability beliefs and subjective well-being. Implications and suggestions for future research were discussed.

The relation between childhood maltreatment and hedonic and eudaimonic well-being in emerging adults: A daily diary study.

BACKGROUND: Prior research has reported that childhood maltreatment is associated with poor well-being, but few studies have examined the association between childhood maltreatment and well-being including hedonic and eudaimonic well-being using a daily diary method. OBJECTIVE: The present study investigated the association between childhood maltreatment and hedonic and eudaimonic well-being, and explored the mediating effects of social support and self-esteem. PARTICIPANTS AND SETTING: Data were collected applying a 14-day daily diary method in two samples. A total of 120 Chinese emerging adults (100 female; Mage = 20.48 years, age range = 18-24 years) and 229 Chinese emerging adults (187 female; Mage = 20.43 years, age range = 18-27 years) comprised the discovery sample and the replication sample, respectively. METHODS: Multilevel regression analysis and multilevel mediation analysis were conducted, while controlling for sex, age, and socioeconomic status. RESULTS: In the discovery sample, the multilevel regression analysis showed that childhood maltreatment had an equal effect on predicting the two types of well-being. Additionally, the multilevel mediation analysis demonstrated that social support and self-esteem acted as independent and equally important mediators of the associations between childhood maltreatment and the two types of well-being. Moreover, the total indirect effect on the childhood maltreatment-hedonic well-being link had no significant difference from that on the childhood maltreatment-eudaimonic well-being link. The replication sample reconfirmed the results of the discovery sample, which provides greater credibility to our findings. CONCLUSIONS: Social support and self-esteem might help to improve the well-being of emerging adults who have suffered childhood maltreatment, and might therefore be important intervention targets.

TRIP13 Induces Nedaplatin Resistance in Esophageal Squamous Cell Carcinoma by Enhancing Repair of DNA Damage and Inhibiting Apoptosis.

Thyroid hormone receptor interactor 13 (TRIP13) plays a crucial role in poor prognosis and chemotherapy resistance of cancer patients. This present study is aimed at investigating the role of high expression of TRIP13 inducing nedaplatin (NDP) resistance in esophageal squamous cell carcinoma (ESCC) cells. High expression of TRIP13 promoted the proliferation and migration of ESCC cells performed by MTS assay, colony formation assay, wound healing assay, and transwell assay. High TRIP13 expression induced NDP resistance to ESCC based on the cell proliferation promoting/inhibition rate and cell migration promoting/inhibition rate analysis, flow cytometry assay of apoptotic subpopulations with a combination of Annexin V-FITC and propidium iodide, and Western blot analysis downregulating cleaved PARP, γH2A.X, cleaved caspase-3, and Bax and upregulating Bcl-2 expression. This study indicated that high expression of TRIP13 promoted proliferation and migration of ESCC cells and induced NDP resistance via enhancing repair of DNA damage and inhibiting apoptosis. This will provide a preliminary reference for the clinical use of NDP in ESCC treatment.

Associations of family subjective socioeconomic status with hedonic and eudaimonic well-being in emerging adulthood: A daily diary study.

Empirical evidence based on retrospective measures has shown that family subjective socioeconomic status (FSSS) was connected to well-being, but few studies have examined this relationship using a daily diary design. Here, we examined the link between FSSS and well-being as well as the mediating roles of social support and self-esteem in a total of 220 emerging adults using a 14-day daily diary design. The multilevel regression analysis found that FSSS positively predicted two types of well-being, including hedonic well-being (HWB) and eudaimonic well-being (EWB). Moreover, the multilevel 2-2-1 mediation analysis indicated that social support and self-esteem performed as independent mediators of the associations between FSSS and two types of well-being. The mediating effect of self-esteem also had no significant difference from that of social support. Furthermore, when age, gender, and family objective socioeconomic status were controlled, these findings remained significant. The implications and limitations of the findings are also discussed.

The complete chloroplast genome sequence of Michelia amoena Q.F.Zheng et M.M.Lin.

Michelia amoena Q.F.Zheng et M.M.Lin is classified in Magnoliaceae and has a high economic value. Herein, we report the complete chloroplast genome of M. amoena using Illumina sequence data. The chloroplast genome is 160,088 bp in length and contains a large single copy (LSC) region of 88,118 bp and a small single-copy (SSC) region of 18,798 bp separated by two inverted repeat (IR) regions of 26,586 bp each. It contained a total of 131 genes, with an overall GC content of 39.26%. The phylogenetic analysis showed that M. amoena is closely related to Michelia figo. This study provides important sequence information for species identification and its phylogenetic relationship in the Magnoliaceae.

Gratitude and depressive symptoms in Chinese nurses: A longitudinal cross-lagged study.

Nurses have a relatively higher rate of depressive symptoms compared to other people. Previous studies have reported that gratitude is correlated with depressive symptoms among healthcare workers. The purpose of this study was to explore the causal relationship between gratitude and depressive symptoms among Chinese nurses. We collected data over 4 months, with 393 nurses at Time 1 and 241 at Time 2. All participants completed online questionnaires. We employed the structural equation models to test the hypotheses. The results revealed that gratitude at Time 1 could predict depressive symptoms at Time 2, and depressive symptoms at Time 1 could predict gratitude at Time 2. These results remained significant after controlling for gender, age, education, marital status, professional title, general positive affect, and negative affect. The findings provide initial evidence about the causal relationship between gratitude and depressive symptoms among Chinese nurses. Hospital administrators can intervene in depressive symptoms of nurses by developing gratitude interventions. Furthermore, nurses with lower levels of depressive symptoms feel more grateful.

The complete chloroplast genome sequence of Casearia glomerata Roxb. (Malpighiales; Salicaceae) from Fujian, China.

Casearia glomerata Roxb. is classified in Salicaceae and has a high economic value. Herein, we report the complete chloroplast (cp) genome of C. glomerata using Illumina sequence data. The cp genome is 156,809 bp in length and contains a large single-copy (LSC) region of 84,888 bp and a small single-copy (SSC) region of 17,039 bp separated by two inverted repeat (IR) regions of 27,441 bp each. It contained a total of 123 genes, with an overall GC content of 36.81%. The phylogenetic analysis showed that C. glomerata is closely related to Casearia velutina. This study provides important sequence information for species identification and its phylogenetic relationship in the Salicaceae.

The complete chloroplast genome of Callicarpa siongsaiensis Metcalf (Lamiaceae) from Fujian Province, China: genome structure and phylogenetic analysis.

Callicarpa siongsaiensis Metcalf is a drought resistance shrub with ornamental potential. In this study, Illumina sequencing data were used to assemble the complete chloroplast genome of Callicarpa siongsaiensis. The length of the circular genome is 154,144 bp. It contains a total of 130 genes, including 87 protein-coding, 36 tRNA, and seven rRNA genes. The GC content of the chloroplast genome of C. siongsaiensis is 38.09%. The phylogenetic analysis fully resolved C. siongsaiensis in a clade with C. formosana.

Validation of the Hedonic and Eudaimonic Motives for Activities-Revised Scale in Chinese Adults.

The Hedonic and Eudaimonic Motives for Activities-Revised scale (HEMA-R) is one of the most extensively used instruments to assess how people pursue well-being. The main aims of the present research were to translate HEMA-R into Chinese and test its construct and predictive validity as well as measurement invariance across gender. In Study 1, we conducted confirmatory factor analysis with data containing 1090 Chinese undergraduates, and replicated the two-factor model which has been found in other studies. Furthermore, the measurement invariance across gender was supported throughout the multi-group confirmatory factor analysis. Study 2 replicated these results and further found the HEMA-R had satisfactory predictive validity in measures of well-being, social support and smartphone addiction. All the findings indicate that the HEMA-R is reliable and valid to measure hedonic and eudaimonic motives, and it could be applied generally across gender in Chinese adults.

Gravity determines the direction of nerve roots sedimentation in the lumbar spinal canal.

OBJECTIVES: To investigate the role of gravity in the sedimentation of lumbar spine nerve roots using magnetic resonance (MR) imaging of various body positions. METHODS: A total of 56 patients, who suffered from back pain and underwent conventional supine lumbar spine MR imaging, were selected from sanmen hospital database. All the patients were called back to our hospital to perform MR imaging in prone position or lateral position. Furthermore, the sedimentation sign (SedSign) was determined based on the suspension of the nerve roots in the dural sac on cross-sectional MR images, and 31 cases were rated as positive and another 25 cases were negative. RESULTS: The mean age of negative SedSign group was significantly younger than that of positive SedSign group (51.7 ± 8.7 vs 68.4 ± 10.5, P < 0.05). The constitutions of clinical diagnosis were significantly different between patients with a positive SedSign and those with a negative SedSign (P < 0.001). Overall, nerve roots of the vast majority of patients (48/56, 85.7%) subsided to the ventral side of the dural sac on the prone MR images, although that of 8 (14.3%) patients remain stay in the dorsal side of dural sac. Nerve roots of only one patient with negative SedSign did not settle to the ventral dural sac, while this phenomenon occurred in 7 patients in positive SedSign group (4% vs 22.6%, P < 0.001). In addition, the nerve roots of all the five patients subsided to the left side of dural sac on lateral position MR images. CONCLUSIONS: The nerve roots sedimentation followed the direction of gravity. Positive SedSign may be a MR sign of lumbar pathology involved the spinal canal.