ABSTRACT
OBJECTIVE: To study the effects of total flavonoids of Hippophae rhamnoides L. (TFH) on the expression of monocyte chemoattractant protein (MCP-1) in aorta of Spontaneously Hypertensive Rats (SHR) and to study the relationship of expression of MCP-1 in aorta and intimal medial thickness (IMT) of aorta. METHODS: Twelve-week-old male SHR (n=12) were randomly devided into three groups to receive TFH [30 mg/(kg x d), n=4], Enalapril [10 mg/(kg x d), n= 4] and Hydrochlorothiazide [25 mg/(kg x d), n=4] for 12 weeks, respectively. Another 4 SHR and 4 WKY which receive placebo served as positive control group and negative control group. The systolic blood pressure (SBP), intimal medial thickness and inside diameter of aorta of the rats were measured. The expression of MCP-1 in aorta was examined by real-time PCR and immunohistochemistry and the concentration of serum MCP-1 protein by ELSA. RESULTS: Twelve weeks later, systolic blood pressure in TFH was significantly decreased, compared with that in Enalapril and Hydrochlorothiazide without statistical differences. TFH markedly reduced the intimal medial thickness of aorta and expression of MCP-1 in aorta, similar with Enalapril, stronger than Hydrochlorothiazide. CONCLUSION: TFH can markedly decrease SBP of SHR and the decrease value of the TFH group was similar with that of the Enalapril and Hydrochlorothiazide group. TFH may inhibit the expression of MCP-1 in aorta and intimal medial thickness of aorta beyond BP Lowering effect. The effect of THF on the expression of MCP-1 and intimal medial thickness of aorta was similar with Enalapril. TFH may through inhibite the expression of MCP-1 in aorta to reduce the intimal medial thickness of aorta and protect hypertensive injury in target organs.
