[A machine learning model on orthokeratology lens fitting based on the data of optometry examination].

Objective: To get an orthokeratology lens fitting model according to the research of the optometry examination data, which can help to improve the work efficiency and increase the hitting rate of prescription. Methods: The relationship between the basic optometry examination data and the effective optometry prescription was evaluated. We got 1 467 sets of data, including 80%(1 173) for training and 20%(294) for testing. The trail results were used for the 20% testing sets, and 100% completed sets for verification. With the contrast of accuracy, we got the influencing variables and the fitting model. Logistic regression and linear regression analysis were applied to the training set for building a fitting model, and the receiver operating characteristic curve (ROC curve) was drawn to determine the optimal threshold of the logistic regression model (1.0).The trail results are used for the 20% testing sets, 100% completed sets for verification. With the contrast of accuracy (ACC), we get the influencing variables and the fitting model. Results: Kf-FK and Ks-SK were selected as dependent variables. Through analysis of Stepwise regression, we found the influencing variables of Kf-FK were Kf(P=0.01), E(mean) (P=0.027), and CYL(P<0.001), with FK=6.23+0.866kf-1.69 E(mean)+0.16CYL, and the precision rate was 81%(r(2)=0.95). The influencing variables of Ks-SK were Ks (P<0.001), CYL (P=0.00), and E(mean) (P<0.001), with SK=6.84+0.862ks-0.28E(mean)-1.3CYL, and the precision rate was 80%(r(2)=0.93). By selecting the logistic method and decision tree analysis for glasses design, the influencing variables were KS (P<0.001)and CYL (P<0.001). The logistic function was Design=-45.7+0.82Ks+6.027CYL, and the precision rate was 87%. Conclusions: The combination of AI and medical data can verify the expert experience and guide the optometrist to locate the prescription quickly and accurately. Try to break the traditional mode of fitting with wearing the orthokeratology lens shortly, and it is possible for us to position the prescription quickly through the calculation of a computer. (Chin J Ophthalmol, 2019, 55: 105-110).

Application of Mismatch Negative in Evaluation of Severity of Mental Disorders due to Traumatic Brain Injury.

ABSTRACT: Objective To explore the applied value of mismatch negative （MMN） in evaluation of severity of mental disorders due to traumatic brain injury. Methods Thirty-five patients（case group） that conform to the diagnostic criteria of organic （traumatic brain injury） mental disorder in ICD-10 Classification of Mental and Behavioural Disorders criteria were selected. Twenty-four healthy subjects （normal control group） that matched the case group in terms of gender, age composition ratio and educational level were selected. All subjects were evaluated by Activity of Daily Living Scale （ADL） and Social Disability Screening Schedule （SDSS） and then examined by Event-Related Potential （ERP）. A statistical analysis of the data was made by SPSS 22.0 software. Results The 32 patients and 24 normal control subjects completed the study. The scores of ADL and SDSS were significantly higher in the case group than in the normal control group （P<0.05）. The latency of Fz, FCz, Cz and Pz in the case group was significantly longer than that in the normal control group （P<0.05）. In the case group, the latency of Fz, FCz, Cz and Pz was positively correlated with the scores of ADL and SDSS （P<0.05）. The equation can be well fitted with the scores of ADL and SDSS. The latency and amplitude of Fz, FCz, Cz and Pz were used as concomitant variables and whether or not the subjects had mental disorders due to traumatic brain injury as dependent variables. Conclusion The latency of MMN can be used as an indicator in potential evaluation of the severity of mental disorders due to traumatic brain injury, which means that the longer the latency of MMN is, the more severe mental disorders due to traumatic brain injury may be. The combined application of ADL, SDSS and MMN can be an objective indicator in preliminary judgment of mental disorders due to traumatic brain injury.

[Applied Value of Electroencephalogram in Assessment of Mild Psychiatry Impairment].

OBJECTIVES: To explore the applied value of electroencephalogram （EEG） in assessment of psychiatric impairment among patients with mental disorders due to traumatic brain injury. METHODS: According to the ICD-10, a total of 271 subjects were enrolled and assessed with the criterion of mental disorders due to traumatic brain injury. Activity of Daily Living Scale （ADL）, Functional Activities Questionnaire （FAQ） and Social Disability Screening Schedule （SDSS） were used to evaluate the severity of patients. All the participants were tested by Wechsler Adult Intelligence Scale （WAIS） and examined by EEG. RESULTS: Totally 215 patients accomplished the study. The results of Glasgow Coma Scale （GCS）, the severity of craniocerebral injury and the scores of FAQ, SDSS and ADL showed significant difference among the patients with different severity of EEG （P<0.05）. The grades of psychiatric impairment showed significant difference among the patients with different abnormal EEG （P<0.05）. CONCLUSIONS: EEG can reflect the severity of craniocerebral injury, assist evaluate the social function and activity of daily living of patients with mental disorders due to traumatic brain injury, and distinguish the mild psychiatric impairment grades, which suggest that EEG has a certain reference value in the assessment of psychiatric impairment.

Diameter-dependent multiferroic functionality in hybrid core/shell NWs.

A versatile approach towards nanofabrication of highly reproducible Co/BiCoO3 (Co/BCO) core/shell (CS) nanowires (NWs) with different diameters has been adopted by demonstrating easily available and low cost sol-gel and electrodeposition routes. X-ray diffraction (XRD) analysis confirmed the tetragonal system of the BCO nanoshells (NSs) with the space group P4mm. Scanning electron microscopy (SEM) clearly demonstrates the uniform morphology with well aligned CS NWs. The magnetization reversal processes (MRPs), experimentally and with analytical modelling, have been discussed for CS NWs with θ ranging from 0° (in-plane magnetic easy axis) to 90° (out-of-plane magnetic hard axis) with magnetic hysteresis loops and geometrical parameters. Crossover from the vortex to transverse reversal mode on increasing θ has been observed for all diameters. An exchange bias effect has been observed for smaller CS NWs diameters and it is attributed to the shell thickness of ∼25 nm. Furthermore, the magnetic anisotropy effect has been discussed in some detail.

Expression of TRAIL and its receptor DR5 and their significance in acute leukemia cells.

We investigated the roles of tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptor death receptor 5 (DR5) in the onset of acute leukemia and changes in their expression during chemotherapy. Bone marrow samples from 16 patients newly diagnosed with acute leukemia were collected before chemotherapy. Bone marrow samples from patients with non-hematologic malignancies served as the control group. Peripheral blood samples of patients with acute leukemia were also collected before chemotherapy and at 1 and 3 days after chemotherapy. Mononuclear cells in the bone marrow and peripheral blood were isolated and used to detect the expression of TRAIL and DR5 by flow cytometry. Compared with mononuclear cells from the control group, mononuclear cells from newly diagnosed patients with acute leukemia showed no significant difference in the expression of TRAIL (P > 0.05) but showed significantly increased expression of DR5 (P < 0.05). TRAIL and DR5 expression in peripheral blood mononuclear cells after chemotherapy was significantly increased compared to expression before chemotherapy (P < 0.05). Patients showing high expression of DR5 had a higher remission rate. One of the mechanisms underlying the treatment of leukemia with chemotherapy drugs may be the induction of TRAIL and DR5, which may promote apoptosis in leukemia cells. TRAIL-mediated apoptosis is regulated by DR5 expression.

Morphological and Molecular Identification of Cytospora germanica Causing Canker on Populus spp. in China.

Species of Cytospora Ehrenb. cause canker and dieback on many genera of hardwoods. During surveys of forest trees in 2004 and 2008, some hardwoods such as Populus spp. with symptoms of canker and dieback were found in Liaoning and Xinjiang provinces, respectively. In these trees, the canker pathogen discolored the sapwood. During wet weather, the conidia were exuded from the fruiting bodies in gelatinous matrices, usually as yellow or orange tendrils. In the spring, the affected trees were wilting and the diffuse cankers spread rapidly and extensively during the period when trees began active spring growth. For saplings, sometimes the death rate of the pathogen has exceeded 50%. Conidiomatal stromata immersed in bark, prominent, circular to ovoid, 1.10 ± 0.23 mm in diameter (n = 10). Discs were white, nearly flat, circular, 0.44 ± 0.04 mm in diameter (n = 10), with one or two ostioles per disc. Ostioles were gray. Locules were subdivided by invaginations into chambers. Conidia were hyaline and lelongate-allantoid shaped, 4 to 5.4 µm long and 1 to 1.4 µm wide. Pieces (5 × 5 mm2) of the junction of affected and healthy tissues were surface sterilized with 1% NaOCl for 30 s and then rinsed twice in sterile distilled water. The fragments were placed on potato dextrose agar (PDA) plates incubated at 25°C for 7 days. The obtained isolates were cultured on PDA at 25°C in diffuse fluorescent light for 30 days, and then were deposited in the culture collection of the Chinese Academy of Forestry. According to these morphological features we initially thought that this pathogen was Cytospora chrysosperma (Pers.) Fr. However, BLAST analysis showed 98% and 99% homology with ITS sequence of isolate CBS118560 (GenBank Accession No. DQ243793), 99% and 100% homology with LSU gene of isolate AR3427 (AF362561) when we amplified ITS-rDNA gene and LSU gene of the isolates. The sequences were submitted to GenBank with the following accession numbers: JQ086563, JQ086564, JX524617, and JX524618. Pathogenicity tests were conducted in the greenhouse by inoculating 20 disinfected (70% ethanol) Populus tomentosa cuttings. Another two cuttings were treated with water agar as controls. The cuttings were incubated at 25°C for 30 days. In 18 of the 20 cuttings, the cambium became brown and appeared water soaked 20 days later, whereas controls did not show any symptoms. C. germanica was reisolated from symptomatic tissues. Inoculations were later repeated two times with similar results. Hubbes (1) and Spielman (2) considered V. germanica (teleomorph of C. germanica) a synonym of V. sordida (teleomorph of C. chrysosperma) based on morphological studies. We almost regarded these isolates as C. chrysosperma too. Morphological identification has been a weak and often inadequate means of identifying Cytospora species (1,2); the present study based on ITS-rDNA gene and LSU gene has substantially elevated identifications. To our knowledge, this is the first report of C. germanica on Populus spp. in China. Populus species are economically important trees in forests of North China and C. germanica has the potential to cause significant economic losses. References: (1) M. Hubbes. Phytopathol. Z. 39:65, 1960. (2) L. J. Spielman. Can. J. Bot. 63:1355, 1985.

Canker on Bark of Populus spp. Caused by Cytospora tritici, a New Disease in China.

Species of Cytospora Ehrenb. and associated teleomorphs cause dieback and canker on over 85 species of angiosperm and gymnosperm plants throughout the world (2). Cytospora tritici Punith. was first observed on Triticum asetivum in Germany in 1980 but may also affect many hardwoods (3). During a survey of landscape trees in 2007, Populus spp. with cankers were found in Fushun, Baoxing, and Luding counties and Chengdu city in Sichuan Province. In these trees, bark canker pathogens discolored the sapwood. During damp weather, conidia were pushed out and formed orange spore horns. Conidiomatal stromata were immersed in bark, prominent, and 1.53 ± 0.33 mm in diameter (n = 10). Discs were white to grey, circular, oval, and 0.59 ± 0.14 mm in diameter (n = 10), with one ostiole per disc. Ostioles were dark grey. Locules were multi-chambered, chambers irregular. Conidia were lelongate-allantoid shaped, hyaline, aseptate, 5.04 ± 0.65 µm long (n = 50), and 1.22 ± 0.13 µm wide (n = 50). Fragments (5 × 5 mm2) of the junction of diseased and healthy tissues were surface sterilized with 1% NaOCl for 30 s and then rinsed twice in sterile distilled water. The pieces were placed on potato dextrose agar (PDA) plates and incubated at 25°C for 7 days. The obtained isolates were cultured on PDA at 25°C in diffuse fluorescent light for 30 days. Upon isolation, the mycelium grew at a rate of 3 to 5 mm per day at 25°C, forming pale white-to-pure white flat colonies. Conidiomata never formed on PDA. ITS1-5.8S-ITS2 sequences were amplified via PCR from genomic DNA obtained from mycelia using universal primers ITS1 and ITS4 (4). The amplification products showed 100% sequence homology with C. tritici isolate DQ243812 from the GenBank database. The ITS sequences were submitted to GenBank (Accession No. JQ277333 to JQ277336). Pathogenicity was confirmed by inoculating 20 disinfected (70% ethanol) Populus tomentosa cuttings. Cuttings were incubated at 25°C for 30 days. Another two cuttings were treated with water agar as controls. In 18 of the 20 cuttings, the cambium developed a brown color and appeared water soaked 15 days later, whereas controls did not develop any symptoms. C. tritici was reisolated from symptomatic tissues. To our knowledge, this is the first report of C. tritici in China causing canker on Populus spp. Cytospora canker is common in practically all countries where poplar are grown. Canker expansion increases when tree defenses are compromised, usually by seasonal dormancy but also by drought, cold injury of wood, sun scald of bark, flooding of root, hail, freezing, or other stress (1). Future spread of C. tritici to western China is considered highly likely. References: (1) G. C. Adams et al. Stud. Mycol. 52:1, 2005. (2) D. F. Farr and A. Y. Rossman. Fungal Databases. Systematic Mycology and Microbiology Laboratory, ARS, USDA. Retrieved from http://nt.ars-grin.gov/fungaldatabases/ March 25, 2012. (3) E. Punithalingam. Nova Hedwigia 32:585, 1980. (4) T. J. White et al. Page 315 in: PCR Protocols: A Guide to Methods and Applications. M. A. Innis et al., eds. Academic Press, San Diego, 1990.