ABSTRACT
A new dammarane-type triterpenoid saponin, (20R)-ginsenoside ST(2) (1), along with five known saponins was isolated from the hongshen extract of Shenmai injection. The structure of 1 was elucidated to be (20R)-dammar-23(E)-ene-3ß,6α,12ß,20,25-pentol 6-O-ß-D-glucopyranoside by means of spectroscopic methods.
Subject(s)
Drugs, Chinese Herbal/isolation & purification , Ginsenosides/isolation & purification , Panax/chemistry , Plants, Medicinal/chemistry , Triterpenes/isolation & purification , Drugs, Chinese Herbal/chemistry , Drugs, Chinese Herbal/pharmacology , Ginsenosides/chemistry , Ginsenosides/pharmacology , Molecular Structure , Nuclear Magnetic Resonance, Biomolecular , Stereoisomerism , Triterpenes/chemistry , Triterpenes/pharmacology , DammaranesABSTRACT
OBJECTIVE: To inverstigate the chemical constituents of Patrinia villosa. METHOD: Under the direction of tetrazolium salt (MTf) assay, the chemical constituents were isolated by silica gel column chromatography and identified by physicochemical properties and spectral analysis (MS, FAB-MS, 1H NMR, 13C NMR). RESULT: Five compounds were isolated from petroleum ether and ethyl acetate extract and identified as: ursolic acid(I), beta-sitosterol (II), patrinalloside(III), kaempferol-3-O-beta-D-glucopyranoside(IV), and kaempferol-3-O-beta-D-glucopyranoside-7-O-alpha-L-rhamnoside(V). CONCLUSION: Compounds I and V were obtained from the plant of genus Patrinia for the first time. Ursolic acid showed powerful anticancer activity through tetrazolium salt(MTT) assay, and its IC50 was 15.0 microg/ml.
