NtHSP70-8b positively regulates heat tolerance and seed size in Nicotiana tabacum.

Heat stress considerably restricts the geographical distribution of crops and affects their growth, development, and productivity. HSP70 plays a critical regulatory role in plant growth response to heat stress. However, the mechanisms of this regulatory remain poorly understood. Here, an HSP70 gene, NtHSP70-8b, which is involved in the heat stress response of tobacco, was cloned and identified. The expression of NtHSP70-8b was induced by exogenous abscisic acid (ABA) treatment and abiotic stress, including heat, drought, and salt. Notably, high NtHSP70-8b expression occurred under heat stress conditions, which was consistent with the ß-glucuronidase histochemical analysis. Moreover, NtHSP70-8b overexpression markedly enhanced heat stress tolerance by changing the stomatal conductance and antioxidant capacity in tobacco leaves. qRT-PCR showed that the expression levels of ABA synthesis and response genes (NtNCED3 and NtAREB), stress defence genes (NtERD10C and NtLEA5), and other HSP genes (NtHSP90 and NtHSP26a) in NtHSP70-8b-overexpressing tobacco were high under heat stress. The interaction of NtHSP70-8b with NtHSP26a was further confirmed by a luciferase complementation imaging assay. In contrast, NtHSP70-8b knockout mutants showed significantly reduced antioxidant capacity compared to the wild type (WT) under heat stress conditions, suggesting that NtHSP70-8b acts as a positive regulator of heat stress in tobacco. Moreover, NtHSP70-8b overexpression increased the 1000-seed weight. Taken together, NtHSP70-8b is involved in the heat stress response, and NtHSP70-8b overexpression contributed to enhanced tolerance to heat stress, which is thus an essential gene with potential application value for developing heat stress-tolerant crops.

EraseNet: End-to-End Text Removal in the Wild.

Scene text removal has attracted increasing research interests owing to its valuable applications in privacy protection, camera-based virtual reality translation, and image editing. However, existing approaches, which fall short on real applications, are mainly because they were evaluated on synthetic or unrepresentative datasets. To fill this gap and facilitate this research direction, this paper proposes a real-world dataset called SCUT-EnsText that consists of 3,562 diverse images selected from public scene text reading benchmarks, and each image is scrupulously annotated to provide visually plausible erasure targets. With SCUT-EnsText, we design a novel GANbased model termed EraseNet that can automatically remove text located on the natural images. The model is a two-stage network that consists of a coarse-erasure sub-network and a refinement sub-network. The refinement sub-network targets improvement in the feature representation and refinement of the coarse outputs to enhance the removal performance. Additionally, EraseNet contains a segmentation head for text perception and a local-global SN-Patch-GAN with spectral normalization (SN) on both the generator and discriminator for maintaining the training stability and the congruity of the erased regions. A sufficient number of experiments are conducted on both the previous public dataset and the brand-new SCUT-EnsText. Our EraseNet significantly outperforms the existing state-of-the-art methods in terms of all metrics, with remarkably superior higherquality results. The dataset and code will be made available at https://github.com/HCIILAB/SCUT-EnsText.

Amplified effect of surface charge on cell adhesion by nanostructures.

Nano-biointerfaces with varied surface charge can be readily fabricated by integrating a template-based process with maleimide-thiol coupling chemistry. Significantly, nanostructures are employed for amplifying the effect of surface charge on cell adhesion, as revealed by the cell-adhesion performance, cell morphology and corresponding cytoskeletal organization. This study may provide a promising strategy for developing new biomedical materials with tailored cell adhesion for tissue implantation and regeneration.