ABSTRACT
OBJECTIVE: To establish a sensitive and specific HPLC fingerprint for the quality controlling of total flavonoids of Folium Apocyni Veneti. METHOD: HPlC analysis was performed on a Kromasil C18 column (4.6 mm x 250 mm, 5 microm) with the mixture of solvent A [acetonitrile-phosphoric acid (95:5)] and solvent B (0.05% phosphoric acid) in gradient mode at a flow rate of 1.0 mL x min(-1). The detection wavelength was set at 360 nm. The column temperature was set at 25 degrees C and the injection volume was 20 microL. RESULT: The chromatographic fingerprint of total flavonoids was established which showed 17 characteristic peaks from 7 patches of total flavonoids products. The similarity from different patches was 0.95-1.00 analyzed by the software of 'Computer-aided Similarity Evaluation' and showed high similitude in peak numbers and the retention time. Moreover, comparison of the HPLC profiles of the total flavonoids with the corresponding Folium Apocyni Veneti leaves indicated that they were closely related to each other. CONCLUSION: The chromatographic fingerprint of the total flavonoids with high specificity and can be used to control its quality and assure the homogenicity for each patch of the total flavonoids.
Subject(s)
Apocynum/chemistry , Chromatography, High Pressure Liquid/methods , Drugs, Chinese Herbal/chemistry , Flavonoids/chemistry , Reproducibility of ResultsABSTRACT
OBJECTIVE: To investigate the purification process of total flavones and total tannins from Apocynum venetum leaves with macroreticular resin. METHOD: The static capacity absorption, dynamic absorption ratio and dynamic elution ratio of different types of macroreticular resins were studied and compared in order to find the best one among the eight macroreticular resins, and the technical process of the type of HPD-400 type macroreticular resin was studied in detail. RESULT: The type of HPD-400 type macroreticular resin showed the best comprehensive absorption property with the following technological conditions: the current velocity was 1 BV x h(-1), the volume of distilled water was 2 BV, the eluting reagent was 60% ethanol, and the volume of 60% ethanol was 3 BV. CONCLUSION: The purity of total flavones and total tannins in the product is up to 80% after purified with HPD-400 macroreticular resin. Therefore, this purification process is feasible.
Subject(s)
Apocynum/chemistry , Chromatography, Liquid/methods , Drugs, Chinese Herbal/isolation & purification , Flavones/isolation & purification , Resins, Synthetic/chemistry , Tannins/isolation & purification , Adsorption , Drugs, Chinese Herbal/chemistry , Flavones/chemistry , Plant Leaves/chemistry , Tannins/chemistryABSTRACT
OBJECTIVE: To study the chemical constituents of flavonoids from Apocynum venetum L. METHODS: The compounds from water extracts were isolated by HPD-100 MCI-gel, polyamide, silica and Sephadex LH-20. Their structrues were characterized by chemical methods and spectroscopic methods. RESULTS: The five compounds were identified to be trifolin (I), hyperroside (III), hyperin (V), isoquercetin-6'-o-acetate (VII) and isoquercetin (VIII). CONCLUSION: Compound I and compound VII are isolated from Apocynum venetum L. for the first time.
