Illuminating plant-microbe interaction: How photoperiod affects rhizosphere and pollutant removal in constructed wetland?

Rhizosphere is a crucial area in comprehending the interaction between plants and microorganisms in constructed wetlands (CWs). However, influence of photoperiod, a key factor that regulates photosynthesis and rhizosphere microbial activity, remains largely unknown. This study investigated the effect of photoperiod (9, 12, 15 h/day) on pollutant removal and underlying mechanisms. Results showed that 15-hour photoperiod treatment exhibited the highest removal efficiencies for COD (87.26%), TN (63.32%), and NO3--N (97.79%). This treatment enhanced photosynthetic pigmentation and root activity, which increased transport of oxygen and soluble organic carbon to rhizosphere, thus promoting microbial nitrification and denitrification. Microbial community analysis revealed a more stable co-occurrence network due to increased complexity and aggregation in the 15-hour photoperiod treatment. Phaselicystis was identified as a key connector, which was responsible for transferring necessary carbon sources, ATP, and electron donors that supported and optimized nitrogen metabolism in the CWs. Structural equation model analysis emphasized the importance of plant-microbe interactions in pollutant removal through increased substance, information, and energy exchange. These findings offer valuable insights for CWs design and operation in various latitudes and rural areas for small-scale decentralized systems.

Exploring the resilience of constructed wetlands to harmful algal blooms disturbances: A study on microbial response mechanisms.

Constructed wetlands (CWs) have emerged as a promising environmentally sustainable technique for wastewater treatment. However, the susceptibility of CWs to disturbances caused by harmful algal blooms (HABs) raises concerns. This study aimed to investigate the impact of HABs on the pollutants' removal performance of CWs and the response of rhizosphere microbial community. Results revealed that CWs possessed an adaptive capacity that enabled them to recover caused by HABs. The rhizosphere was found to stimulate the occurrence of Acinetobacter, which played a critical role to help resist HABs disturbance. This study also observed an increased dissimilatory nitrate reduction metabolic pathway which promoted denitrification and enhanced the nitrogen removal efficiency of CWs. Additionally, the structural equation model further suggested that dissolved oxygen exerted a significant influence on the microbial activities and then affected the pollutants removal performance. Overall, our findings shed light on the mechanism for CW stability maintenance during HABs disturbance.

Microalgae-based constructed wetland system enhances nitrogen removal and reduce carbon emissions: Performance and mechanisms.

Combination of constructed wetlands (CWs) and microalgae-based technologies has been proved as effective wastewater treatment option; however, little attention was paid to investigate the optimal combination ways. This study showed that the integrated system (IS) connecting microalgal pond with CWs exhibited improved pollutant-removal efficiencies and preferred carbon reduction effects compared to other alternatives such as coupled system or independent CWs. Microbial analysis demonstrated that core microorganisms (e.g., Acinetobacter and Thermomonas) of the IS were mostly associated with carbon, nitrogen, and energy metabolism. Based on co-occurrence networks, microbial quantity with denitrification function in the IS accounted for 71.01 % of the microorganism related to nitrogen metabolism, which was higher than that of 48.84 % in the independent CWs, indicating that the presence of microalgae in IS played important role in promoting biological denitrification. These findings provide insights into the microbial mechanism and highlights the complementary effects between microalgae and CWs.

Succession dynamics of microbial communities responding to the exogenous microalgae ZM-5 and analysis of the environmental sustainability of a constructed wetland system.

Constructed wetlands (CWs) are economical and effective swine tailwater treatment systems. However, nitrogen removal in CWs is limited by the lack of carbon source for denitrification. In this study, we studied the feasibility of dosing the microalgae ZM-5 to improve the nitrogen removal ability in CWs. Compared to the control CW, a 20 % higher removal capacity of COD and TN was observed for the coupled system (EG). The microalgae ZM-5 could interact with denitrifying bacteria to compensate for the deficiency of denitrifying stage in CWs. HT-qPCR chip analysis also provided evidence that denitrification genes significantly increased (p < 0.05). According to the life cycle assessment (LCA), ultrasonic extraction had the best environmental sustainability among four lipid extraction processes. As an improvement strategy, clean energy could be utilized to optimize the electricity source to reduce environmental load (45 %-60 %). These findings offer new insights into the feasibility of EG for environmentally sustainable wastewater treatment.

Successional dynamics of microbial communities in response to concentration perturbation in constructed wetland system.

Constructed wetlands (CWs) are widely considered as resilient systems able to adapt to environmental perturbations. Little attention has been paid, however, to microbial dynamics when CWs withstand and recover from external shock. To understand the resilience of CWs, this study investigated rhizosphere microbial dynamics when CWs were subjected to influent COD perturbation (200 mg/L-1600 mg/L). Results demonstrated that CWs had strong adaptability to different influent perturbations, characterized by transitions from fluctuating to stable pollutant removal. Microbial analysis showed that rhizosphere microorganisms competed for niches in response to increased COD concentrations, and Trichococcus played key roles in resisting concentration perturbations. Structural equation modeling indicated that rhizosphere community succession and microbial energy metabolism were shaped by pH and DO. These findings provide insights into the mechanism for CW stability maintenance when facing concentration perturbations.