Magnetic Ion-Imprinted Materials for Selective Adsorption of Cr(VI): Adsorption Behavior and Mechanism Study.

With the increase of hexavalent Cr(VI) wastewater discharged from industrial production, it seriously pollutes water bodies and poses a risk to human health. Adsorption is used as an effective means to treat Cr(VI), but its effectiveness is affected by pH, and the adsorption performance decreases when acidity is strong. Furthermore, research on the mechanism of Cr(VI) adsorption using DFT calculations needs to be developed. This study focuses on the development of magnetically responsive core-shell nano-ion imprinted materials (Fe3O4@GO@IIP) through magnetic separation and surface imprinting techniques. Characterization techniques including FT-IR, XRD, and EDS confirmed the core-shell nanostructure of Fe3O4@GO@IIP. Batch adsorption experiments and model simulations demonstrated the exceptional adsorption capacity of Fe3O4@GO@IIP for Cr(VI) in strongly acidic solutions (pH = 1), reaching a maximum of 89.18 mg/g. The adsorption mechanism was elucidated through XPS and DFT calculations, revealing that Fe3O4@GO@IIP operates through electrostatic interactions and chemical adsorption, with charge transfer dynamics quantified during the process. This research provides new insights for addressing Cr(VI) treatment in highly acidic environments.

Construction of cryomicroneedles loaded with milk-derived exosomes encapsulated TNF-α siRNA and efficacy of percutaneous acupoint administration in rheumatoid arthritis.

Inhibiting the expression of tumor necrosis factor-α (TNF-α), a pro-inflammatory cytokine widely distributed in the serum and synovial fluid, is important for managing rheumatoid arthritis (RA). Despite the good therapeutic effects of TNF-α small interfering RNA (TNF-α siRNA) in RA animal models, safe and efficient siRNA delivery systems that retain stability are lacking. We introduced a novel therapy using milk-derived exosomes(mEXOs)-encapsulated TNF-α siRNA-coated cryomicroneedle (cryoMN) patch and evaluated its efficacy via local transdermal administration through acupoints in RA treatment. The loading of TNF-α siRNAs into mEXOs was achieved by sonication, the loading rate, stability, and in vitro release of mEXOs-TNF-α siRNA were determined. The cryoMNs were prepared by micromolding, morphology, drug loading, and mechanical strength of the cryoMN array were analyzed. The loading efficiency of TNF-α siRNA was up to 21% and each cryoMN contained 39.6 ± 1.29 µg of TNF-α siRNA. Frozen sections penetrated 523 ± 63 µm deep. In vitro experiments have shown that mEXOs-TNF-α siRNA cryoMNs have good biocompatibility and inhibit the proliferation of HFLS-RA cells. In vivo pharmacodynamics studies found that general conditions, changes in microcirculation indexes, synovial histopathological changes, and expression of related proteins in the synovial tissue in RA rabbits were effectively alleviated by mEXOs-TNF-α siRNA cryoMNs. Improvement of each index at acupoints was greater than that at non-acupoints. Our findings facilitate the development of cryoMNs combined with exosomes and acupoints drug delivery for the treatment of RA. The combination of exosomes and cryoMNs will enable the development of new-generation microneedle-based treatments.

Green, recyclable and high latent heat form-stable phase change composites supported by cellulose nanofibers for thermal energy management.

Efficiently addressing the challenge of leakage is crucial in the advancement of solid-liquid phase change thermal storage composite materials; however, numerous existing preparation methods often entail complexity and high energy consumption. Herein, a straightforward blending approach was adopted to fabricate stable phase change nanocomposites capitalizing on the interaction between TEMPO-oxidized cellulose nanofibers (TOCNF) and polyethylene glycol (PEG) molecules. By adjusting the ratio of TOCNF to PEG and the molecular weights of PEG, TOCNF/PEG phase change composites (TPCC) with customizable phase transition temperature (40.3-59.1 °C) and high phase transition latent heat (126.3-172.1 J/g) were obtained. The TPCC of high-loaded PEG (80-95 wt%) ensured a leakage rate of less than 1.7 wt% after 100 heating-cooling cycles. Moreover, TPCC exhibits excellent optical properties with a transmittance of over 90 % at room temperature and up to 96 % after heating. The thermal response analysis of TPCC demonstrates exceptional thermal-induced flexibility and good thermal stability, as well as recyclability and reshaping ability. This study may inspire others to design bio-based phase change composites with potential applications in thermal energy storage and management of smart-energy buildings, photothermal response devices, and waste heat-generating electronics.

Polarization-dependent photoinduced metal-insulator transitions in manganites.

In correlated oxides, collaborative manipulation on light intensity, wavelength, pulse duration and polarization has yielded many exotic discoveries, such as phase transitions and novel quantum states. In view of potential optoelectronic applications, tailoring long-lived static properties by light-induced effects is highly desirable. So far, the polarization state of light has rarely been reported as a control parameter for this purpose. Here, we report polarization-dependent metal-to-insulator transition (MIT) in phase-separated manganite thin films, introducing a new degree of freedom to control static MIT. Specifically, we observed giant photoinduced resistance jumps with striking features: (1) a single resistance jump occurs upon a linearly polarized light incident with a chosen polarization angle, and a second resistance jump occurs when the polarization angle changes; (2) the amplitude of the second resistance jump depends sensitively on the actual change of the polarization angles. Linear transmittance measurements reveal that the origin of the above phenomena is closely related to the coexistence of anisotropic micro-domains. Our results represent a first step to utilize light polarization as an active knob to manipulate static phase transitions, pointing towards new pathways for nonvolatile optoelectronic devices and sensors.

Traditional Chinese exercises on pain and disability in middle-aged and elderly patients with lumbar disc herniation: a systematic review and meta-analysis of randomized controlled trials.

Background: Traditional Chinese exercises (TCEs) have played a significant role in treating various diseases. However, there is limited research assessing the efficacy of TCEs in treating Lumbar disc herniation (LDH). This study aimed to systematically evaluate the effects of four commonly used TCEs (Baduanjin, Yijinjing, Taichi, and Wuqinxi) on pain and disability in elderly patients with LDH. Objectives: To assess the quality of relevant randomized controlled trials (RCTs) to provide evidence support for the treatment of LDH. Methods: RCTs were identified through eight databases. Meta-analysis and trial sequence analysis (TSA) were conducted using RevMan 5.4, Stata 17.0, and TSA 0.9. Results: A total of 22 RCTs, involving 1931 patients, were included in the analysis. TCEs exhibited a superior effectiveness in treating LDH compared to the control group. However, the TSA analysis suggested the possibility of false positives, indicating the need for more high-quality RCT evidence. Nevertheless, TCEs showed reliable results in significantly improving the VAS score and JOA score of LDH patients. Conclusion: Current evidence indicates that the four TCEs have advantages in treating LDH in middle-aged and elderly individuals. However, considering the limitations of this study, we need to exercise caution in drawing conclusions, and further research is required to validate these findings. Systematic Review Registration: http://www.crd.york.ac.uk/PROSPERO, identifier [CRD42023431633].

[Recent advances in nanocarrier-based drug delivery systems in treatment of rheumatoid arthritis].

Rheumatoid arthritis(RA) is a widely prevalent autoimmune inflammatory disease that severely affects patients' quality of life. Currently, conventional formulations against RA have several limitations, such as nonspecificity, poor efficacy, large drug dosages, frequent administration, and systemic side effects. Nanotechnology-based drug delivery systems have emerged as a promising stra-tegy for the diagnosis and treatment of RA since nanotechnology can overcome the limitations of traditional treatments and simplify the complexity of the disease. These systems enable targeted delivery of anti-inflammatory drugs to the inflamed areas through active and passive targeting, achieving specificity to the joints, overcoming the need for increased dosage and administration frequency, and reducing associated adverse reactions. This article aimed to review nanocarrier-based drug delivery systems in the field of RA and elucidate how nanosystems can be utilized to deliver therapeutic drugs to inflamed joints for controlling RA progression. By discussing the current issues and challenges faced by nanodrug delivery systems and highlighting the urgent need for solutions, this article offers theoretical support for further research on nanotechnology-based co-delivery systems in the future.

[Salvianolic acid A contributes to cartilage endplate cell restoration by regulating miR-940 and miR-576-5p].

OBJECTIVE: To investigate whether Salvianolic acid A (SAA) can restore cartilage endplate cell degeneration of intervertebral discs and to identify the mechanism via regulation of micro-RNA. METHODS: Cartilage endplate cells were isolated from lumbar intervertebral disc surgical samples and were treated with serum containing a series of concentrations of SAA (2, 5, and 10 ?M) for 24, 48, and 72 h to identify a proper dose and treatment time of SAA. The effect SAA on interlenkin-1ß (IL-1ß)-induced extracellular matrix degradation of cartilage endplate cells were analyzed by Alcian blue staining and assessment of the expression levels of ADAMTS-5, MMP3 and Col2a1. Further, the potential target miRNAs were preliminarily screened by micro-RNA sequencing combining qRT-PCR and Western blot, and then, the miRNAs mimics and inhibitors were used to verify the regulatory effect of SAA on potential target miRNAs. RESULTS: The 10 µM SAA treatment for 48 h significantly enhanced the viability of cartilage endplate cells, and increased Col2a1 expression and glycosaminoglycan accumulation that were repressed by IL-1ß, and reduced the effect of IL-1ß on ADAMTS-5, and MMP3. Screening analysis based on micro-RNA sequencing and Venny analysis identified the downstream micro-RNAs, including miR-940 and miR-576-5p. Then, the miR-940-mimic or miR-576-5p-mimic were transfected into CEPCs. Compared with the SAA group, the expression of ADAMTS-5 and MMP3 increased significantly and the expression of COL2A1 obviously decreased after overexpression of miR-940 or miR-576-5p in CEPCs. CONCLUSION: Salvianolic acid A attenuated the IL-1ß-induced extracellular matrix degradation of cartilage endplate cells by targeting regulate the miR-940 and the miR-576-5p.

Facile Design of Flexible, Strong, and Highly Conductive MXene-Based Composite Films for Multifunctional Applications.

Strong, conductive, and flexible materials with improving ion accessibility have attracted significant attention in electromagnetic interference (EMI) and foldable wearable electronics. However, it still remains a great challenge to realize high performance at the same time for both properties. Herein, a microscale structural design combined with nanostructures strategy to fabricate TOCNF(F)/Ti3 C2 Tx (M)@AgNW(A) composite films via a facile vacuum filtration process followed by hot pressing (TOCNF = TEMPO-oxidized cellulose nanofibrils, NW = nanowires) is described. The comparison reveals that different microscale structures can significantly influence the properties of thin films, especially their electrochemical properties. Impressively, the ultrathin MA/F/MA film with enhanced layer in the middle exhibits an excellent tensile strength of 107.9 MPa, an outstanding electrical conductivity of 8.4 × 106 S m-1 , and a high SSE/t of 26 014.52 dB cm2 g-1 . The assembled asymmetric MA/F/MA//TOCNF@CNT (carbon nanotubes) supercapacitor leads to a significantly high areal energy density of 49.08 µWh cm-2 at a power density of 777.26 µW cm-2 . This study proposes an effective strategy to circumvent the trade-off between EMI performance and electrochemical properties, providing an inspiration for the fabrication of multifunctional films for a wide variety of applications in aerospace, national defense, precision instruments, and next-generation electronics.

MIL-161 Metal-Organic Framework for Efficient Au(III) Recovery from Secondary Resources: Performance, Mechanism, and DFT Calculations.

The recovery of precious metals from secondary resources is significant economically and environmentally. However, their separation is still challenging because they often occur in complex metal ion mixtures. The poor selectivity of adsorbents for gold in complicated solutions prevents further application of adsorption technology. In this study, a Zr-based MOF adsorbent, MIL-161, was synthesized using s-tetrazine dicarboxylic acid (H2STz) as an organic ligand. MIL-161 demonstrated a high adsorption capacity of up to 446.49 mg/g and outstanding selectivity for gold(III) in a simulated electronic waste solution as a result of the presence of sulfur- and nitrogen-containing groups. In addition, the MIL-161 adsorbents were characterized using Fourier transform infrared (FT-IR), field emission scanning electron microscopy (FESEM), thermogravimetric analysis (TG), Brunner-Emment-Teller (BET), and X-ray photoelectron spectroscopy (XPS). Additionally, the adsorption kinetics, isotherms, and thermodynamics of the MOF adsorbents were also thoroughly examined. More importantly, the experimental results and DFT calculations indicate that chelation and electrostatic interactions are the main adsorption mechanisms.

[Treatment of rheumatoid arthritis by injection of sinomenine solid lipid nanoparticles under a fluorescence endoscopic laser confocal microscope].

A fluorescence endoscopic laser confocal microscope(FELCM) was used to direct the injection of sinomenine solid lipid nanoparticles(Sin-SLN) into the joint, and the in vitro effectiveness of Sin-SLN in the treatment of rheumatoid arthritis(RA) was evaluated. Sin-SLN was prepared with the emulsion evaporation-low temperature curing method. The Sin-SLN prepared under the optimal conditions showed the encapsulation efficiency of 64.79%±3.12%, the drug loading of 3.84%±0.28%, the average particle size of(215.27±4.21) nm, and the Zeta potential of(-32.67±0.84) mV. Moreover, the Sin-SLN demonstrated good stability after sto-rage for 30 days. The rabbit model of RA was established by the subcutaneous injection of ovalbumin and complete Freund's adjuvant. Five groups were designed, including a control group, a model group, a Sin(1.5 mg·kg~(-1)) group, a Sin-SLN(1.5 mg·kg~(-1)) group, and a dexamethasone(positive drug, 1.0 mg·kg~(-1), ig) group. The control group and the model group only received puncture treatment without drug injection. After drug administration, the local skin temperature and knee joint diameter were monitored every day. The knee joint diameter and the local skin temperature were lower in the drug administration groups than in the model group(P<0.05, P<0.01). FELCM recorded the morphological alterations of the cartilage of knee joint. The Sin-SLN group showed compact tissue structure and smooth surface of the cartilage. Enzyme-linked immunosorbent assay(ELISA) was employed to determine the serum le-vels of interleukin-1(IL-1) and tumor necrosis factor-α(TNF-α). The findings revealed that the Sin-SLN group had lower IL-1 and TNF-α levels than the model group(P<0.05, P<0.01). Hematoxylin-eosin(HE) staining was employed to reveal the pathological changes of the synovial tissue, which were significantly mitigated in the Sin-SLN group. The prepared Sin-SLN had uniform particle size and high stability. Through joint injection administration, a drug reservoir was formed. Sin-SLN effectively alleviate joint swelling and cartilage damage of rabbit, down-regulated the expression of inflammatory cytokines, and inhibited the epithelial proliferation and inflammatory cell infiltration of the synovial tissue, demonstrating the efficacy in treating RA.

Selective Adsorption Behavior and Mechanism for Cd(II) in Aqueous Solution with a Recoverable Magnetie-Surface Ion-Imprinted Polymer.

A novel recoverable magnetic Cd(II) ion-imprinted polymer was synthesized on the surface of silica-coated Fe3O4 particles via the surface imprinting technique and chemical grafting method. The resulting polymer was used as a highly efficient adsorbent for the removal of Cd(II) ions from aqueous solutions. The adsorption experiments revealed that Fe3O4@SiO2@IIP had a maximum adsorption capacity of up to 29.82 mg·g-1 for Cd(II) at an optimal pH of 6, with the adsorption equilibrium achieved within 20 min. The adsorption process followed the pseudo-second-order kinetic model and the Langmuir isotherm adsorption model. Thermodynamic studies showed that the adsorption of Cd(II) on the imprinted polymer was spontaneous and entropy-increasing. Furthermore, the Fe3O4@SiO2@IIP could rapidly achieve solid-liquid separation in the presence of an external magnetic field. More importantly, despite the poor affinity of the functional groups constructed on the polymer surface for Cd(II), we improved the specific selectivity of the imprinted adsorbent for Cd(II) through surface imprinting technology. The selective adsorption mechanism was verified by XPS and DFT theoretical calculations.

The Facile and Efficient Fabrication of Rice Husk/poly (lactic acid) Foam Composites by Coordinated the Interface Combination and Bubble Hole Structure.

The possibility of agricultural-forestry waste (rice husks) and biodegradable plastics (poly(lactic acid)) being used to produce ecologically friendly foam composite was discussed in this work. The effects of different material parameters (the dosage of PLA-g-MAH, type and content of chemical foaming agent) on the microstructure and physical properties of composite were investigated. PLA-g-MAH promoted the chemical grafting between cellulose and PLA, and made the structure denser, thus improving the interface compatibility of the two phases and resulting in good thermal stability, high tensile strength (6.99 MPa) and bending strength (28.85 MPa) of composites. Furthermore, the properties of rice husk/PLA foam composite prepared by two kinds of foaming agents (endothermic and exothermic) were characterized. The addition of fiber limited the growth of pores, which provided better dimensional stability and narrower pore size distribution, made the interface of the composite bond tightly. And the bubble can prevent crack propagation and improve the mechanical properties of the composite. The bending strength and tensile strength of composite were 37.36 MPa and 25.32 MPa, which increased by 28.35 % and 23.27 %, respectively. Therefore, the composite prepared by using agricultural-forestry wastes and poly(lactic acid) possess acceptable mechanical properties, thermal stability and water resistance, expanding the scope of application.

[PK/PD model of Chuanxiong gel plaster in treatment of rheumatoid arthritis].

In this experiment, the PK/PD fitting model of Chuanxiong(Chuanxiong Rhizoma) in the treatment of rheumatoid arthritis was established in the form of acupoint combined with external application gel paste. Firstly, the rheumatoid arthritis model was induced by ovalbumin, and the articular fluid of rabbits was extracted by microdialysis. The pharmacokinetic process of Chuanxiong in rabbit articular fluid was analyzed by UPLC-MS/MS, and the pharmacokinetic model was established. The pharmacodynamic effects of Chuanxiong on inflammatory factors IL-1ß, TNF-α, and IL-6 were analyzed by enzyme-linked immunosorbent assay(ELISA). The pharmacodynamic model was established, and the PK/PD model was obtained by fitting the data of pharmacokinetics and pharmacodynamics. The results of pharmacokinetics showed that the concentration of ligustrolide A in the articular cavity by drug administration on classical acupoint Zusanli(ST 36) was higher than that by Yanglingquan(GB 34), which reflected the advantage of typical acupoint, while ligustrazine concentration was higher after administration through Yanglingquan than through Zusanli, which was different from the traditional acupoint theory. The results of pharmacodynamics showed that the drug had lag effect. The PK/PD model was constructed by fitting the data. When IL-1ß was taken as the efficacy index, the PK/PD models of Chuanxiong in typical acupoint Zusanli group, atypical acupoint Yanglingquan group, and non-acupoint group were E=115.28C_e/(3 316.72+C_e), E=108.73C_e/(2 993.47+C_e), and E=101.34C_e/(3 028.51+C_e). When TNF-α was taken as the efficacy index, the PK/PD models of Chuanxiong in typical acupoint Zusanli group, atypical acupoint Yanglingquan group, and non-acupoint group were E=68.31C_e/(3 285.16+C_e), E=59.27C_e/(2 919.86+C_e), and E=53.61C_e/(2 862.87+C_e). When IL-6 was taken as the efficacy index, the PK/PD models of Chuanxiong in typical acupoint Zusanli group, atypical acupoint Yanglingquan group, and non-acupoint group were E=59.92C_e/(3 461.17+C_e), E=58.34C_e/(2 723.51+C_e), and E=49.17C_e/(2 862.76+C_e). The parameters showed that there were significant differences in E_(max), EC_(e50) and k_(eo). The analysis of data found that the PK/PD fitting effect of Zusanli, a typical acupoint, was the best, which proved that it was still the best site for drug administration. To sum up, it shows that there may be bidirectional selectivity between drugs and acupoints.

Selective removal and recovery of Ni(ii) using a sulfonic acid-based magnetic rattle-type ion-imprinted polymer: adsorption performance and mechanisms.

It is significant to selectively remove Ni(ii) ions from wastewater. A novel sulfonic acid-based magnetic rattle-type ion-imprinted polymer (Fe3O4@void@IIP-Ni(ii)) was designed by taking advantage of the strong interaction between Ni(ii) and sulfonic acid groups. Green polymerization was used to synthesize Fe3O4@void@IIP-Ni(ii), which was then investigated using SEM, TEM, FT-IR, VSM, TGA, EDS, and XPS. The adsorption results indicated that the prepared imprinted material had a short adsorption equilibrium time (10 min), good magnetic responsiveness (about 5 seconds) and high adsorption capacity (44.64 mg g-1) for Ni(ii) at the optimal pH of 6.0. The removal rate of Ni(ii) was up to 99.97%, and the adsorption process was spontaneous and endothermic, following the pseudo-secondary kinetic model and Langmuir model. The selectivity coefficients of the imprinted material were 4.67, 4.62, 8.94 and 9.69 for Ni(ii)/Co(ii), Ni(ii)/Cu(ii), Ni(ii)/Pb(ii) and Ni(ii)/Zn(ii), respectively. The regeneration and application of the imprinted material in actual water samples have been verified. Moreover, the mechanism of selective adsorption for Ni(ii) was investigated by FTIR, XPS and density functional theory (DFT) calculation. The results showed that the imprinted sorbent has a strong binding ability with Ni(ii), and the adsorption of Ni(ii) on Fe3O4@void@IIP-Ni(ii) was the result of the co-coordination of O atoms of the sulfonic acid groups and N atoms of -N-C[double bond, length as m-dash]O groups in AMPS with Ni(ii).

Machine learning guided microwave-assisted quantum dot synthesis and an indication of residual H2O2 in human teeth.

The current preparation methods of carbon quantum dots (CDs) involve many reaction parameters, which leads to many possibilities in the synthesis processes and high uncertainty of the resultant production performance. Recently, machine learning (ML) methods have shown great potential in correlating the selected features in many applications, which can help understand the relevant structure-function relationships of CDs and discover better synthesis recipes as well. In this work, we employ the ML approach to guide the blue CD synthesis in microwave systems. After optimizing the synthesis parameters and conditions, the quantum yield (QY) increases to about 200% higher than the average value of the prepared samples without ML guidance. The obtained CDs are applied as fluorescent probes to monitor hydrogen peroxide (H2O2) in human teeth. The CD probe exhibits a linear relationship with the concentration of H2O2 ranging from 0 to 1.1 M with a lower detection limit of 0.12 M, which can effectively detect the residual H2O2 after bleaching teeth. This work shows that the adopted ML methods have considerable advantages in guiding the synthesis of high-quality CDs, which could accelerate the development of other novel functional materials in energy, biomedical, and environmental remediation applications.

Mn3+/Mn4+ ion-doped carbon dots as fenton-like catalysts for fluorescence dual-signal detection of dopamine.

Carbon dots (CDs), a new zero-dimensional material, have ignited a revolution in the fields of sensing, bioimaging, and biomedicine. However, the difficulty of preparing CDs with Fenton-like catalytic properties has seriously hindered their application in the diagnosis of oxidation/reduction biomolecules or metal ions. Here, an innovative method was successfully established to synthesize Mn3+/Mn4+ ion-doped blue-green fluorescent CDs with Fenton-like catalytic properties using manganese acetate as the manganese source. Specifically, the CDs prepared here were equipped with functional groups of -COOH, NH2, C=O, and Mn-O, offering the possibility to function as a fluorescence sensor. More importantly, the introduction of manganese acetate resulted in the preparation of CDs with Fenton-like catalytic properties, and the dual-signal fluorescence detection of dopamine (DA) was realized with linear ranges of 100-275 nM and 325-525 nM, and the detection limits were 3 and 12 nM, respectively. In addition, due to the Fenton-like catalytic activity of Mn3+/Mn4+ ion-doped CDs, the material has broad application prospects in the detection of oxidation/reduction biomolecules or metal ions related to disease diagnosis and prevention.

Ultrathin cellulose nanofiber/carbon nanotube/Ti3C2Tx film for electromagnetic interference shielding and energy storage.

Controllable fabrication of lightweight, highly conductive, and flexible films is important to simultaneously achieve excellent electromagnetic interference (EMI) shielding and high-rate energy storage. Herein, ultrathin, flexible, and conductive (up to 365,000 ± 5000 S m-1) TOCNFs/CNT/Ti3C2Tx hybrid films were fabricated by a facile vacuum-filtration. The obtained films with 60 wt% Ti3C2Tx content exhibited a high specific EMI SE of 9316.4 ± 205.32 dB cm2 g-1, which was comparable to most of the other carbon- and MXene- based materials synthesized by complex steps. Additionally, the porous structure contributed to exposing more active sites and providing efficient transport of electrolyte ions. Consequently, the hybrid films showed a high areal capacitance and high specific capacitance of 537 mF cm-2 and 279.7 F g-1 at 0.3 mA cm-2, respectively, together with impressive stability of 93.1% after 8000 cycles. This work provides an effective strategy to synthesize high-performance conductive films for applications in wearable or portable electronic devices.

Targeted Repair of Vascular Injury by Adipose-Derived Stem Cells Modified with P-Selectin Binding Peptide.

Percutaneous coronary intervention for coronary artery disease treatment often results in pathological vascular injury, characterized by P-selectin overexpression. Adipose-derived stem cells (ADSCs) therapeutic efficacy remains elusive due to poor ADSCs targeting and retention in injured vessels. Here, conjugated P-selectin binding peptide (PBP) to polyethylene glycol-conjugated phospholipid derivative (DMPE-PEG) linkers (DMPE-PEG-PBP; DPP) are used to facilitate the modification of PBP onto ADSCs cell surfaces via hydrophobic interactions between DMPE-PEG and the phospholipid bilayer. DPP modification neither has influence on ADSCs proliferation nor apoptosis/paracrine factor gene expression. A total of 5 × 10-6 m DPP-modified ADSCs (DPP-ADSCs) strongly binds to P-selectin-displaying activated platelets and endothelial cells (ECs) in vitro and to wire-injured rat femoral arteries when administered by intra-arterial injection. Targeted binding of ADSCs shields injury sites from platelet and leukocyte adhesion, thereby decreasing inflammation at injury sites. Furthermore, targeted binding of ADSCs recovers injured ECs functionality and reduces platelet-initiated vascular smooth muscle cells (VSMCs) chemotactic migration. Targeted binding of DPP-human ADSCs to balloon-injured human femoral arteries is also demonstrated in ex vivo experiments. Overall, DPP-ADSCs promote vascular repair, inhibit neointimal hyperplasia, increase endothelium functionality, and maintain normal VSMCs alignment, supporting preclinical noninvasive utilization of DPP-ADSCs for vascular injury.

CYC1, SDHA, UQCRC1, UQCRQ, and SDHB might be important biomarkers in kidney transplant rejection.

BACKGROUND: Kidney transplant rejection is considered as a vital factor of kidney transplant failure. Therefore, it's necessary to search for effective biomarkers for kidney transplant surveillance. METHODS: In this study, we conducted time-series gene expression profiles analysis of samples from kidney transplant patients with different post-transplant days through weighted gene co-expression network analysis (WGCNA). Associations between gene co-expression modules and days post-transplant were determined through spearman rank correlation analysis. Potential kidney transplant rejection-related modules were subjected to gene functional enrichment analysis through clusterProfiler and protein-protein interaction analysis via STRING database. RESULTS: A total of 11 gene co-expression modules were identified, and the pink module which was mainly involved in "energy derivation by oxidation of organic compounds" and "Huntington disease" showed significant correlation with the phenotypic trait "days post-transplant". CYC1, SDHA, UQCRC1, UQCRQ, and SDHB in the pink module exhibited high scores in the protein-protein interaction network analysis. CONCLUSIONS: We reported several potential genes may be associated with the kidney transplant rejection, which should provide novel biomarkers for kidney transplant surveillance.