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1.
Zhen Ci Yan Jiu ; 46(12): 1043-7, 2021 Dec 25.
Article in Chinese | MEDLINE | ID: mdl-34970882

ABSTRACT

OBJECTIVE: To observe the effect and safety of injection of vitamin B1 into Zusanli (ST36) and Hegu (LI4) in the treatment of functional dyspepsia (FD). METHODS: A total of 100 FD patients were equally divided into medication group and acupoint injection group (n=50/group) according to a random number table. Patients in the medication group were ordered to take mosapride citrate tablets (5 mg) orally 30 min before each of the three meals, while those in the acupoint injection group received injection of vitamin B1 into ST36 and LI4, once every other day, three times a week. One week later, the clinical symptom scores, total effective rates, serum gastrin (GAS) and plasma motilin (MTL) contents, and gastric emptying rates between the two groups were compared, followed by the observation of adverse reactions. Two weeks' follow-up survey was conducted after the end of treatment, and the clinical symptom scores and total effective rates of the two groups were further compared. RESULTS: Compared with the data before treatment in the same one group, the clinical symptom scores and serum GAS contents of post-treatment as well as the follow-up symptom scores were all significantly decreased (P<0.05,P<0.01), while the plasma MTL levels and gastric emptying rates were obviously increased in both groups (P<0.01). Comparison between the two groups showed that the clinical symptom score, serum GAS content after the treatment and follow-up symptom score were considerably lower (P<0.01), but the total effective rate, plasma MTL, gastric emptying rate after the treatment and total effective rate of follow-up notably higher in the acupoint injection group than those in the medication group (P<0.01,P<0.05). CONCLUSION: Injection of vitamin B1 into ST36 and LI4 is effective in improving symptoms of PD patients, which may be related to its functions in regulating the levels of GAS and MTL in blood, and facilitating gastrointestinal motility.


Subject(s)
Acupuncture Therapy , Dyspepsia , Acupuncture Points , Dyspepsia/drug therapy , Humans , Motilin , Thiamine
2.
Transbound Emerg Dis ; 68(4): 2250-2260, 2021 Jul.
Article in English | MEDLINE | ID: mdl-33048441

ABSTRACT

Anthrax is a natural foci disease in Inner Mongolia, which poses a severe threat to public health. In this study, the incidence number, rate and constituent ratio were used to describe the epidemiological characteristics of anthrax in the region from 1956-2018. The molecular correlation and genetic characteristics of the strains were investigated using canonical single nucleotide polymorphisms (CanSNP), multiple-locus variable-number tandem repeat analysis (MLVA-15) and whole genome sequencing (WGS). The epidemiological characteristics of anthrax in Inner Mongolia have altered significantly. The incidence of anthrax has decreased annually without vaccination, and the regional distribution of anthrax gradually transferred from central and western regions to the eastern. Moreover, the occupation distribution evolved from multiple early occupations to predominated by farmers and herdsmen. This change is closely related to policy factors and to changes in the means of production and the living habits of the local population. This indicates that reformulating the control and prevention strategies is essential. Both A. Br. Ames and A. Br. 001/002 subgroups were the predominant CanSNP genotypes of Bacillus anthracis in Inner Mongolia. A total of 36 strains constituted six shared MLVA-15 genotypes, suggesting an epidemiological link between the strains of each shared genotype. The six shared genotypes ([GT1, 9, 11 and 15] and [GT8 and 12]) consisting of 2-7 strains confirmed the occurrence of multiple point outbreaks and cross-regional transmission caused by multiple common sources of infection. Phylogenetic analysis based on the WGS core genome showed that strains from this study formed an independent clade (C.V.), and they were positioned close to each other, suggesting a common origin. Further comparison analysis should be performed to ascertain the geographic origin of these strains.


Subject(s)
Anthrax , Bacillus anthracis , Animals , Anthrax/epidemiology , Anthrax/veterinary , Bacillus anthracis/genetics , China/epidemiology , Genotype , Minisatellite Repeats/genetics , Molecular Epidemiology , Phylogeny , Polymorphism, Single Nucleotide
3.
Reproduction ; 160(3): 343-351, 2020 09.
Article in English | MEDLINE | ID: mdl-32580158

ABSTRACT

Embryo implantation is a crucial step for the successful establishment of mammalian pregnancy. Cyclophilin A (CYPA) is a ubiquitously expressed intracellular protein and is secreted in response to inflammatory stimuli to regulate diverse cellular functions. However, there are currently no reports about the role of CYPA in embryo implantation. Here, we examine the expression pattern of CYPA during mouse early pregnancy and explore the potential role of CYPA during implantation. CYPA is expressed in the subluminal stroma surrounding the implanting blastocyst on day 5 of pregnancy, but not at inter-implantation sites. In ovariectomized mice, estrogen and progesterone significantly stimulate CYPA expression. When pregnant mice are injected intraperitoneally with CYPA inhibitor, the numbers of implantation sites are significantly reduced. Using an in vitro stromal cell culture system, Ppia siRNA knockdown of CYPA and CYPA-specific inhibitor treatment partially inhibits levels of CD147, MMP3 and MMP9. Decreased CYPA expression also significantly inhibits Stat3 activity and expands estrogen responsiveness. Taken together, CYPA may play an important role during mouse embryo implantation.


Subject(s)
Cyclophilin A/metabolism , Embryo Implantation , Gene Expression Regulation, Developmental , STAT3 Transcription Factor/metabolism , Animals , Cyclophilin A/genetics , Female , Gonadal Steroid Hormones/metabolism , Mice , Pregnancy , STAT3 Transcription Factor/genetics , Stromal Cells/cytology , Stromal Cells/metabolism , Uterus/cytology , Uterus/metabolism
4.
Cell Biol Int ; 40(12): 1340-1348, 2016 Dec.
Article in English | MEDLINE | ID: mdl-27730697

ABSTRACT

Bone loss is a critical pathology responsible for the functional disability in patients with rheumatoid arthritis (RA). It is well known that receptor activator of nuclear factor kappa-B (NF-κB) ligand (RANKL) plays a crucial role in bone loss in RA. The purpose of this study was to determine whether recombinant human endostatin (rh-endostatin) mediates bone erosion in RA by regulation of RANKL expression in an experimental model of RA, consisting of mice with adjuvant-induced arthritis (AA). Cultured AA fibroblast-like synoviocytes (FLSs) obtained from these mice were induced by tumor necrosis factor-α (TNF-α) combined with or without rh-endostatin. The levels of RANKL and osteoprotegerin (OPG) mRNA, soluble and membrane-bound proteins were assessed by real-time PCR, ELISA, and Western blotting. Western blotting and the luciferase reporter assay were used to study related signaling pathways. Rh-endostatin inhibited RANKL mRNA expression, soluble and membrane-bound protein expression in AA FLSs but not in CD4+ T cells. However, OPG expression and secretion was not affected by rh-endostatin in AA FLSs. Molecular analysis demonstrated that rh-endostatin significantly inhibited TNF-α-induced MAPK and AP-1 signaling pathways. Moreover, rh-endostatin attenuated TNF-α-induced NF-κB signaling by suppressing the phosphorylation level of inhibitor kappaBα (IκBα) and nuclear translocation of NF-κB p65 in FLSs from mice with AA. These results provide the first evidence that rh-endostatin inhibits TNF-α-induced RANKL expression in AA FLSs.


Subject(s)
Arthritis, Experimental/drug therapy , Endostatins/therapeutic use , Fibroblasts/pathology , RANK Ligand/metabolism , Recombinant Proteins/therapeutic use , Synoviocytes/pathology , Tumor Necrosis Factor-alpha/pharmacology , Animals , Arthritis, Experimental/metabolism , Arthritis, Experimental/pathology , CD4-Positive T-Lymphocytes/drug effects , CD4-Positive T-Lymphocytes/metabolism , Cell Nucleus/drug effects , Cell Nucleus/metabolism , Cells, Cultured , Endostatins/pharmacology , Fibroblasts/drug effects , Fibroblasts/metabolism , Humans , I-kappa B Proteins/metabolism , MAP Kinase Signaling System/drug effects , Male , Mice , Mice, Inbred BALB C , Models, Biological , Osteoprotegerin/metabolism , Phosphorylation/drug effects , Protein Transport/drug effects , Recombinant Proteins/pharmacology , Synoviocytes/drug effects , Synoviocytes/metabolism , Transcription Factor AP-1/metabolism , Transcription Factor RelA/metabolism
5.
Echocardiography ; 32(1): 174-7, 2015 Jan.
Article in English | MEDLINE | ID: mdl-25251665

ABSTRACT

Fibrous cyst of the chordae tendineae of the mitral valve (MV) is defined as a fibrous cyst arising from the chordae tendineae of the MV. It is extremely rare and its etiology is not clear. We present a case of a cystic structure within the left ventricle. This structure is connected to the anterior MV leaflet and the posterior chordae tendineae. It moves freely, resulting in stenosis of inflow tract and outflow tract of the left ventricle. Intraoperative assessment and histopathologic examination revealed it as a fibrous cyst. Its echocardiographic appearance is unique and it must be resected immediately.


Subject(s)
Chordae Tendineae/diagnostic imaging , Cysts/diagnostic imaging , Echocardiography/methods , Mitral Valve Insufficiency/diagnostic imaging , Ventricular Outflow Obstruction/diagnostic imaging , Adolescent , Cysts/complications , Cysts/surgery , Humans , Male , Mitral Valve/diagnostic imaging , Mitral Valve Insufficiency/complications , Mitral Valve Insufficiency/surgery , Treatment Outcome , Ventricular Outflow Obstruction/etiology , Ventricular Outflow Obstruction/surgery
6.
Mol Cell Endocrinol ; 381(1-2): 272-9, 2013 Dec 05.
Article in English | MEDLINE | ID: mdl-23994020

ABSTRACT

Embryo implantation requires a precise synchronism between the receptive uterus and activated blastocyst and is regulated by complicated molecular networks. Although many implantation-related genes have been identified, the crosstalk among them is still unknown. Snail, a transcription repressor, plays a central role during epithelial-mesenchymal transition. Our previous study showed that Snail is highly expressed at implantation site in mouse uterus. This study was to examine how Snail is related with other implantation-related genes in mice. Uterine stromal cells were isolated from mouse uteri on day 4 of pregnancy and treated with HB-EGF. Snail was induced significantly by HB-EGF. By using specific inhibitors and siRNA, we demonstrated that HB-EGF induction on Snail expression is dependent on the EGFR-ERK-Stat3 pathway. Cox-2 was regulated by Snail. The current findings demonstrate that Snail can relate with HB-EGF, Stat3 and Cox-2 and may play a role during mouse embryo implantation and decidualization.


Subject(s)
Decidua/metabolism , Intercellular Signaling Peptides and Proteins/physiology , Transcription Factors/genetics , Transcriptional Activation , Animals , Cells, Cultured , Cyclooxygenase 2/genetics , Cyclooxygenase 2/metabolism , Embryo Implantation , ErbB Receptors/metabolism , Female , Heparin-binding EGF-like Growth Factor , MAP Kinase Signaling System , Male , Mice , Phosphorylation , Pregnancy , Protein Processing, Post-Translational , STAT3 Transcription Factor/metabolism , Snail Family Transcription Factors , Transcription Factors/metabolism
7.
Fertil Steril ; 100(5): 1410-8, 2013 Nov.
Article in English | MEDLINE | ID: mdl-23909989

ABSTRACT

OBJECTIVE: To determine Claudin-3 expression and its regulatory factors during embryo implantation. DESIGN: Experimental mouse models and cell culture. SETTING: University research laboratory. ANIMAL(S): Sexually mature female CD-1 strain mice. INTERVENTION(S): Ovariectomy and treatments. MAIN OUTCOME MEASURE(S): In situ hybridization and immunohistochemistry for detecting Claudin-3 messenger RNA and protein expression in mouse uterus, respectively; Western blot for detecting protein levels; immunofluorescence for detecting Claudin-3 protein in cultured cells. RESULT(S): Claudin-3 is strongly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy, and diminished at day 5 implantation sites. Then it is expressed at secondary decidual zone on day 8. Pseudopregnant uteri have a similar expression pattern as pregnant uteri from days 1-5. Claudin-3 expression is down-regulated after delayed implantation is activated by estrogen (E) treatment. Meanwhile Claudin-3 expression is stimulated by artificial decidualization. In ovariectomized mice, P induces Claudin-3 expression in the luminal epithelium, which is abrogated by P receptor antagonist RU486. Heparin-binding-epidermal growth factor (HB-EGF) down-regulates Claudin-3 expression, but enhances transcription factor Snail expression. In human endometrial epithelial ECC-1 cells, both E and P could stimulate Claudin-3 expression, whereas HB-EGF decreases Claudin-3 and increases Snail expression. CONCLUSION(S): Claudin-3 expression in uterine luminal epithelium is stimulated by P and suppressed by HB-EGF in mice and humans.


Subject(s)
Claudin-3/metabolism , Epithelial Cells/metabolism , Intercellular Signaling Peptides and Proteins/metabolism , Progesterone/metabolism , Tight Junctions/metabolism , Uterus/metabolism , Animals , Cells, Cultured , Claudin-3/genetics , Embryo Implantation, Delayed , Epithelial Cells/drug effects , Estrogens/metabolism , Female , Gene Expression Regulation , Heparin-binding EGF-like Growth Factor , Hormone Antagonists/pharmacology , Mice , Ovariectomy , Pregnancy , Receptors, Progesterone/antagonists & inhibitors , Receptors, Progesterone/metabolism , Snail Family Transcription Factors , Tight Junctions/drug effects , Time Factors , Transcription Factors/metabolism , Uterus/drug effects
8.
Reprod Sci ; 20(4): 354-60, 2013 Apr.
Article in English | MEDLINE | ID: mdl-23302397

ABSTRACT

The epithelial-mesenchymal transition plays a critical role in embryonic development, cancer progression, and metastasis. Decidualization is the process by which the fibroblast-like endometrial stromal cells differentiate into polygonal epithelial-like cells. However, it is still unclear whether mesenchymal-epithelial transition (MET) occurs during decidualization. The aim of this study was to examine whether decidualization causes the downregulation of some mesenchymal markers and upregulation of some epithelial markers in cultured uterine stromal cells. We showed that decidualization causes the downregulation of snail and vimentin expression, and upregulation of E-cadherin and cytokeratin expression. During in vitro decidualization, cultured stromal cells lose elongated shape and show epithelium-like characteristics. Our data suggest that the process of MET may exist during decidualization.


Subject(s)
Decidua/cytology , Decidua/physiology , Epithelial-Mesenchymal Transition/physiology , Animals , Cells, Cultured , Female , Humans , Mice , Pregnancy
9.
Reprod Sci ; 19(9): 923-31, 2012 Sep.
Article in English | MEDLINE | ID: mdl-22556012

ABSTRACT

Interleukin 1 (IL-1) is one of the most potent proinflammatory cytokines possessing a wide spectrum of inflammatory, metabolic, hemopoietic, and immunologic properties. In addition, the IL-1 system has been considered relevant in regulating communication between the blastocyst and the endometrium. Interleukin 1 receptor type II (IL1R2) acts as a negative regulator for IL-1 actions and has been termed a "decoy receptor." The aim of this study was to determine the expression pattern of IL1R2 gene in mouse uterus during the early pregnancy. Both in situ hybridization and immunohistochemistry were performed to examine the spatial localization of IL1R2 expression in mouse uteri. Real-time quantitative polymerase chain reaction analyses were used to quantify Il1r2 messenger RNA (mRNA) level under in vivo and in vitro artificial decidualization. By transfecting Il1r2 gene in cultured stromal cells from day 4 pregnant mice, we detected the expression of Dtprp, a well-known marker for decidualization. Our results showed that IL1R2 gene expression was mainly localized in decidual cells close to the implanting embryo during days 5 to 8 of pregnancy. Under in vivo and in vitro artificial decidualization, Il1r2 was significantly upregulated. Dtprp mRNA expression was also upregulated by Il1r2 overexpression. Our data suggest that IL1R2 may play an important role during mouse decidualization.


Subject(s)
Decidua/metabolism , Gene Expression Regulation, Developmental , Receptors, Interleukin-1 Type II/biosynthesis , Animals , Female , Mice , Pregnancy , RNA, Messenger/biosynthesis
10.
11.
Funct Plant Biol ; 38(6): 441-450, 2011 Jun.
Article in English | MEDLINE | ID: mdl-32480899

ABSTRACT

Protein kinases are signal transduction factors that play a central role in acclimation. In this study, the function of a calcium sensor-interacting protein kinase, OsCIPK03, was characterised in the salt stress response of rice (Oryza sativa L.). Transgenic plants overexpressing OsCIPK03 exhibited an increased sensitivity to salt stress during both seed germination and seedling growth. By contrast, transgenic RNA interference lines that underexpressed OsCIPK03 were significantly more tolerant to NaCl stress than the wild-type. In response to salt stress, rice that underexpressed OsCIPK03 accumulated more proline than non-transformed plants. Furthermore, several stress-responsive genes were identified as being differentially expressed in the transgenic plants. Together, these results suggest that OsCIPK03 functions as a negative regulator of salt stress tolerance in rice.

12.
Endocrinology ; 151(10): 5007-16, 2010 Oct.
Article in English | MEDLINE | ID: mdl-20668027

ABSTRACT

Embryo implantation is an intricate interaction between receptive uterus and active blastocyst. The mechanism underlying embryo implantation is still unknown. Although histamine and putrescine are important for embryo implantation and decidualization, excess amount of histamine and putrescine is harmful. Amiloride binding protein 1 (Abp1) is a membrane-associated amine oxidase and mainly metabolizes histamine and putrescine. In this study, we first showed that Abp1 is strongly expressed in the decidua on d 5-8 of pregnancy. Abp1 expression is not detected during pseudopregnancy and under delayed implantation but is detected after estrogen activation. Because Abp1 is mainly localized in the decidua and also strongly expressed during in vitro decidualization, Abp1 might play a role during mouse decidualization. The regulation of estrogen on Abp1 is mediated by transcription factor CCAAT/enhancer-binding protein-ß. Abp1 expression is also regulated by cAMP, bone morphogenetic protein 2, and ERK1/2. Abp1 may be essential for mouse embryo implantation and decidualization.


Subject(s)
Amine Oxidase (Copper-Containing)/genetics , CCAAT-Enhancer-Binding Protein-beta/physiology , D-Amino-Acid Oxidase/genetics , Decidua/drug effects , Embryo Implantation/drug effects , Estrogens/pharmacology , Uterus/drug effects , Amiloride/metabolism , Amine Oxidase (Copper-Containing)/metabolism , Amine Oxidase (Copper-Containing)/physiology , Animals , CCAAT-Enhancer-Binding Protein-beta/metabolism , Cells, Cultured , D-Amino-Acid Oxidase/metabolism , D-Amino-Acid Oxidase/physiology , Decidua/metabolism , Embryo Implantation/genetics , Female , Gene Expression Regulation/drug effects , Gestational Age , Gonadal Steroid Hormones/pharmacology , Male , Mice , Pregnancy/genetics , Pregnancy/metabolism , Uterus/metabolism
13.
Zhongguo Zhen Jiu ; 27(12): 875-80, 2007 Dec.
Article in Chinese | MEDLINE | ID: mdl-18271226

ABSTRACT

OBJECTIVE: To probe into clinical therapeutic effect of acupuncture on diabetic nephropathies and the mechanism. METHODS: Using multi-central, randomized and blind methods, 130 cases of diabetic nephropathy were divided into an observation group and a control group, 65 cases in each group. They were treated by routine diabetic therapy, and in the observation group, acupuncture at Quchi (LI 11), Zhigou (TE 6), Hegu (LI 4), Xuehai (SP 10), Zusanli (ST 36), Yinlingquan (SP 9), Fenglong (ST 40), Diji (SP 8), Sanyinjiao (SP 6), Taichong (LR 3), Tianshu (ST 25), Gaohuang (BL 43), Shenshu (BL 23), Zhongwan (CV 12), Zhongji (CV 3) were added with needling method of harmonizing spleen-stomach. While in the control group, acupuncture at Shenshu (BL 23), Taixi (KI 3), Sanyingjiao (SP 6), Yanglingquan (GB 34), Xuanzhong (GB 39), Guanyuan (CV 4), Shousanli (LI 10), Waiguan (TE 5), Yangxi (LI 5), Liangqiu (ST 34), Shangjuxu (ST 37), Neiting (ST 44), Huaroumen (ST 24), Dachangshu (BL 25). The treatment was given twice a day in the two groups. Clinical therapeutic effects were assessed according to clinical symptoms and signs, blood sugar, blood lipids, urinary albumin excretion rate, urinary monocyte chemotactic protein-1 (MCP-1), glomerular filtration rate (GFR), renal blood flow, etc.. RESULTS: The needling method of harmonizing spleen-stomach not only could improve symptoms and signs of the patients, and also had benign regulative action on metabolism of blood sugar and lipids, and GFR, renal blood flow and urinary albumin level, with significant or very significant differences as compared with the control group ( P < 0.05 or P < 0.01). CONCLUSION: The needling method of harmonizing spleen-stomach is an effective method for diabetic nephropathies, which can improve progressive renal lesion induced by abnormal metabolism of blood sugar and lipids, improve renal blood flow and GFR, decrease urinary albumin secretion, inhibit over expression of MCP-1, protect glomerulus and renal tubules, so as to improve renal function and delay renal lesion.


Subject(s)
Acupuncture Therapy/methods , Diabetic Nephropathies/therapy , Medicine, Chinese Traditional , Aged , Blood Glucose/analysis , Blood Urea Nitrogen , Female , Glomerular Filtration Rate , Humans , Kidney/physiopathology , Lipids/blood , Male , Middle Aged , Spleen/physiopathology , Stomach/physiopathology
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