ABSTRACT
This study determined the physicochemical changes and bacterial community succession in the pig manure composting process with microbial inoculant. Microbial inoculant could prolong the thermophilic stage by 2â¯days and increased the germination index (GI). Analysis with 16S rDNA showed that the Chao1 and Shannon indices increased at the thermophilic stage in the treatment (T), while those of the control (C) decreased. Microbial inoculant increased the relative abundance of Flavobacterium and Solibacillus in 4-12 and 12-24â¯days, respectively. Acinetobacter was reduced at 4-12â¯days. The key physicochemical factors affecting microbial successions were revealed by canonical correspondence analysis (CCA) and correlation analysis. Linear discriminant analysis (LDA) effect size (LEfse) analysis showed that there were 78 biomarkers, while in piles T and C, there were 35 and 43 biomarkers, respectively. These results indicated the addition of microbial inoculant improved the maturity and fertility, as well as significantly regulating the microbial community structure.
