ABSTRACT
The aim of the present study was to construct a single-chain bispecific antibody (scBsAb) against cervical carcinoma and to investigate its biological activities. The scBsAb was constructed using a genetic cloning technique and antigen binding activities were detected by ELISA. The iodogen method was used to analyze the pharmacokinetics. The Rosette formation test was used to detect the binding ability between peripheral blood lymphocytes (PBLs) and Cs1213 cervical cancer cells. In addition, the MTT method was performed to detect the killing effect of PBLs. The molecular weight of the scBsAb was ~60 kDa. The antigen binding activities of scBsAbs were compared with the antihuman cervical carcinoma antibody singlechain Fv fragment (CSAs1 scFv) and anticluster of differentiation (CD)3 scFv (P>0.05). In addition, a pharmacokinetics assay demonstrated that compared with the two corresponding scFvs, scBsAbs exhibited a significantly prolonged retention time in the body (P<0.01). In addition, the number of rosettes formed by PBLs and Cs1213 cells in the scBsAb group was markedly greater than that in the scFv groups or the RPMI1640 group (P<0.05 and P<0.01, respectively). The killing activity of PBLs against scBsAbmediated Cs1213 cells was significantly greater than that mediated by the other antibodies (P<0.05). When the concentration of scBsAb was 40 µg/ml, the killing rate was 64.5%. Thus, antihuman cervical carcinoma/antiCD3 scBsAbs may possess two types of antigen binding activity, prolong the duration in vivo and improve the killing activity of PBLs against cancer cells.
Subject(s)
Antibodies, Bispecific/genetics , Antibodies, Bispecific/immunology , Antigens, Neoplasm/immunology , CD3 Complex/immunology , Single-Chain Antibodies/genetics , Single-Chain Antibodies/immunology , Uterine Cervical Neoplasms/immunology , Antibodies, Bispecific/isolation & purification , Antibodies, Bispecific/pharmacokinetics , Antibody-Dependent Cell Cytotoxicity , Cell Line, Tumor , Female , Gene Expression , Gene Order , Genetic Vectors , Humans , Hybridomas , Protein Binding/immunology , Single-Chain Antibodies/isolation & purification , Single-Chain Antibodies/pharmacokineticsABSTRACT
OBJECTIVE: To investigate the prevalence and subtype distribution of human papillomavirus (HPV) infection and its correlation with age among women in Beijing urban area, and provide some epidemiological evidence for the clinical application of HPV vaccines. METHODS: We collected cervical specimens from 1999 women in the Outpatient Department of our hospital, performed genetyping of HPV-DNA, and analyzed the incidence of HPV infection in different age groups. RESULTS: HPV infection was detected in 502 (25.2%) of the 1999 women patients, with 391 (19.6%) cases of high-risk HPV, which included 326 (83.4%, 326/391) cases of single infection. HPV-16 was the most common type (21.2%, 69/326), followed by HPV-52 (19.3%, 63/326) and HPV-58 (16.0%, 52/326). The prevalence of HPV infection was the highest among the women aged 41 -50 years and the lowest among those over 60 years. CONCLUSION: The subtype- and age-specific distribution of HPV infection among women in Beijing urban area shows an obvious heterogeneity, which deserves due consideration in the clinical application of HPV vaccines.
