FIGNL1 Promotes Hepatocellular Carcinoma Formation via Remodeling ECM-receptor Interaction Pathway Mediated by HMMR.

BACKGROUND: The development of novel biomarkers is crucial for the treatment of HCC. In this study, we investigated a new molecular therapeutic target for HCC. Fidgetin-like 1 (FIGNL1) has been reported to play a vital role in lung adenocarcinoma. However, the potential function of FIGNL1 in HCC is still unknown. OBJECTIVE: This study aims to investigate the key regulatory mechanisms of FIGNL1 in the formation of HCC. METHODS: The regulatory effect of FIGNL1 on HCC was studied by lentivirus infection. In vitro, the effects of FIGNL1 on the proliferation, migration and apoptosis of cells were investigated by CCK8, colony formation assay, transwell and flow cytometry. Meanwhile, the regulation of FIGNL1 on HCC formation in vivo was studied by subcutaneous transplanted tumors. In addition, using transcriptome sequencing technology, we further explored the specific molecular mechanism of FIGNL1 regulating the formation of HCC. RESULTS: Functionally, we demonstrated that FIGNL1 knockdown significantly inhibited HCC cell proliferation, migration and promoted cell apoptosis in vitro. Similarly, the knockdown of FIGNL1 meaningfully weakened hepatocarcinogenesis in nude mice. Transcriptome sequencing revealed that FIGNL1 affected the expression of genes involved in extracellular matrix-receptor (ECM-receptor) interaction pathway, such as hyaluronan mediated motility receptor (HMMR). Further validation found that overexpression of HMMR based on knockdown FIGNL1 can rescue the expression abundance of related genes involved in the ECM-receptor interaction pathway. CONCLUSION: Our study revealed that FIGNL1 could modulate the ECM-receptor interaction pathway through the regulation of HMMR, thus regulating the formation of HCC.

The effect of spin-orbit coupling on molecular properties: Potential energy curve, transition dipole moment and laser cooling scheme of NH.

The influence of spin-orbit coupling on the cooling of NH molecular laser is investigated based on the ab initio theory. The potential energy curves (PECs) and spectral constants for four Λ-S states (X3Σ-, a1Δ, b1Σ+ and A3Π) and eight Ω states [Formula: see text] a1Δ2, [Formula: see text] and [Formula: see text] ) of NH molecule are obtained by the multi-reference configuration interaction (MRCI) plus Davidson correction. The spectroscopic constants (Re, ωe, ωeχe, Be, De) are obtained by solving the one dimensional radial Schrödinger equation, and the results are almost the same as the previously reported data. In addition, the transition dipole moment, permanent dipole moment, Franck-Condon factors, and radiative lifetime of NH molecule are also acquired. Also, the effects of the intermediate state on the [Formula: see text] , [Formula: see text] and [Formula: see text] transitions are considered. The feasible laser cooling schemes using a single laser are formulated. In the proposed cooling scheme, there wavelengths for the [Formula: see text] are used, the main pump lasers are λ00 = 335.74 nm. The feasible transition is based on this. It is found that spin-orbit coupling has a significant effect on potential energy curves, permanent dipole moments, transition dipole moments and vibration energy levels.

Catalytically active gold clusters with atomic precision for noninvasive early intervention of neurotrauma.

BACKGROUND: Neurotrauma is a worldwide public health problem which can be divided into primary and secondary damge. The primary damge is caused by external forces and triggers the overproduction of peroxides and superoxides, leading to long-lasting secondary damage including oxidative stress, wound infection and immunological reactions. The emerging catalysts have shown great potential in the treatment of brain injury and neurogenic inflammation, but are limited to biosafety issues and delivery efficiency. RESULTS: Herein, we proposed the noninvasive delivery route to brain trauma by employing highly active gold clusters with enzyme-like activity to achieve the early intervention. The decomposition rate to H2O2 of the ultrasmall gold clusters is 10 times that of glassy carbon (GC) electrodes, indicating excellent catalytic activity. The gold clusters can relieve the oxidative stress and decrease the excessive O2·- and H2O2 both in vitro and in vivo. Besides, gold clusters can accelerate the wound healing of brain trauma and alleviate inflammation via inhibiting the activation of astrocytes and microglia through noninvasive adminstration. decrease the peroxide and superoxide of brain tissue. CONCLUSIONS: Present work shows noninvasive treatment is a promising route for early intervention of brain trauma.

Recent Progress on NIR-II Photothermal Therapy.

Photothermal therapy is a very promising treatment method in the field of cancer therapy. The photothermal nanomaterials in near-infrared region (NIR-I, 750-900 nm) attracts extensive attention in recent years because of the good biological penetration of NIR light. However, the penetration depth is still not enough for solid tumors due to high tissue scattering. The light in the second near-infrared region (NIR-II, 1000-1700 nm) allows deeper tissue penetration, higher upper limit of radiation and greater tissue tolerance than that in the NIR-I, and it shows greater application potential in photothermal conversion. This review summarizes the photothermal properties of Au nanomaterials, two-dimensional materials, metal oxide sulfides and polymers in the NIR-II and their application prospects in photothermal therapy. It will arouse the interest of scientists in the field of cancer treatment as well as nanomedicine.

A hybrid feature-based patient-to-image registration method for robot-assisted long bone osteotomy.

PURPOSE: The purpose of this study is to provide a simple, feasible and effective patient-to-image registration method for robot-assisted long bone osteotomy, which has rarely been systematically reported. The practical requirement is to meet the accuracy of 1 mm or even higher without bone-implanted markers. METHODS: A hybrid feature-based registration method termed CR-RAMSICP is proposed. Point-based coarse registration (CR) is accomplished relying on the optical retro-reflective markers attached to the tracked rigid body fixed out of the bone. In surface-based fine registration, an improved iterative closest point (ICP) algorithm based on the range-adaptive matching strategy (termed RAMSICP) is presented to cope with the robust precise matching between the asymmetric patient and image point clouds, which avoids converging to a local minimum. RESULTS: A series of registration experiments based on the isolated porcine iliums are carried out. The results illustrate that CR-RAMSICP not only significantly outperforms CR and CR-ICP in the accuracy and reproducibility, but also exhibits better robustness to the CR errors and less sensitiveness to the distribution and number of fiducial points located in the patient point cloud than CR-ICP. CONCLUSION: The proposed registration method CR-RAMSICP can stably satisfy the desired registration accuracy without the use of bone-implanted markers like fiducial screws. Besides, the RAMSICP algorithm used in fine registration is convenient for programming because any complex metrics or models are not involved.

Molybdenum disulfide-based materials with enzyme-like characteristics for biological applications.

Nanozyme, a kind of nanomaterials with enzymatic activity, has been developing vigorously over the past years owing to its advantages such as low-cost, easy storage, ease of use in harsh environments and so on, compared with natural enzymes. At present, as a typical two-dimensional nanomaterial, molybdenum disulfide (MoS2) and their hybrids with unexpected enzyme-like activities have caused wide attention. In this review, we mainly investigated the enzyme-like activities of MoS2 based nanomaterials, including peroxidase-like activity, catalase-like activity and superoxide dismutase-like activity. Furthermore, we systematically introduce recent research progress of MoS2 based nanomaterials in the fields of biological applications such as radiation protection, cancer therapy, antibacterial, and wound healing. Finally, the current challenges and perspectives of MoS2 based nanomaterials in the future are also discussed and proposed. We expect this review may be significant to understand the properties of MoS2 based nanomaterials and the development of two-dimensional nanomaterials with enzyme mimicking activities.

Enzyme-Like Properties of Gold Clusters for Biomedical Application.

In recent years, the rapid development of nanoscience and technology has provided a new opportunity for the development and preparation of new inorganic enzymes. Nanozyme is a new generation of artificial mimetic enzyme, which like natural enzymes, can efficiently catalyze the substrate of enzyme under mild conditions, exhibiting catalytic efficiency, and enzymatic reaction kinetics similar to natural enzymes. However, nanozymes exist better stability than native enzymes, it can still maintain 85 % catalytic activity in strong acid and alkali (pH 2~10) or large temperature range (4~90°C). This provides conditions for designing complex catalytic systems. In this review, we discussed the enzymatic attributes and biomedical applications of gold nanoclusters, including peroxidase-like, catalase-like, detection of heavy metal ions, and therapy of brain and cancer etc. This review can help us understand the current research status nanozymes.

Multiband circular dichroism from bilayer rotational F4 nanostructure arrays.

A chiral nanostructure array is designed, which is composed of a bilayer rotational F4-shaped nanoarray configuration. The surface plasmon resonance and circular dichroism are studied by changing the parameters of the structure. The results show that the structure has strong multiband circular dichroism, which is attributed to the coupling of the layers. In theory, based on the Born-Kuhn model, the upper and lower nanostructures are equivalent to electric dipoles. By analyzing the coupling mode of electric dipoles in the upper and lower layer, the mechanism of circular dichroism and the shift of the circular dichroism resonance are revealed. Besides, there are several specific modes that are fault tolerant of fabrication issues. This feature unveils the bright prospect of spectral anti-interference. So, the suggested chiral nanostructure can be used in biologically targeted molecular detection and spectral sensing.

Laser cooling of the OH- molecular anion in a theoretical investigation.

The schemes for laser cooling of the OH- anion are proposed using an ab initio method. Scalar relativistic corrections are considered using the Douglas-Kroll Hamilton. Spin-orbit coupling (SOC) effects are taken into account at the MRCI+Q level. SOC effects play important roles in the transition properties of the OH- anion. Transition strengths for the transition of the OH- anion cannot be ignored. Large vibrational branching ratios for the and transitions are determined. Short spontaneous radiative lifetimes for the a3Π1 and A1Π1 states are also predicted for rapid laser cooling. The vibrational branching loss ratio to the intervening states a3Π0 and a3Π1 for the transition is small enough to enable the building of a laser cooling project. The three required laser wavelengths for the and transitions are all in the visible region. The results imply the probability of laser cooling of the OH- anion via both a spin-forbidden transition and a three-electronic-level transition.

Theoretical investigation on spin-forbidden cooling transitions of gallium hydride.

Herein, the spin-forbidden cooling of a gallium hydride molecule is investigated using ab initio quantum chemistry. The cooling transition and the corresponding potential energy curves including , a3Π0-, a3Π0+, a3Π1, a3Π2, A1Π1, , 13Σ, , , and 23Σ states are simulated based on the multi-reference configuration interaction approach plus Davidson corrections method. By solving the nuclear Schrödinger equation, we calculate the spectroscopic constants of these states, which are in good agreement with the available experimental values. Based on the transition data, there seems to be a theoretical puzzle: highly diagonally distributed Franck-Condon factor f00 for transitions , , and for the gallium hydride molecule but the intervening state A1Π1 for transition is prohibitive to laser cooling. In addition, the transition does not have a suitable rate of optical cycling owing to a large radiative lifetime for state. Our theoretical simulation indicates the solution to the puzzle: the transition has a high emission rate, and there is a suitable radiative lifetime for a3Π1 state, which can ensure rapid and efficient laser cooling of gallium hydride. The proposed laser drives transition by using three wavelengths (main pump laser λ00; two repumping lasers λ10 and λ21). These results demonstrate the possibility of laser-cooling the gallium hydride molecule, and a sub-microkelvin cool temperature can be reached for this molecule.

Dressed multi-wave mixing process with Rydberg blockade.

We investigate the way to control multi-wave mixing (MWM) process in Rydberg atoms via the interaction between Rydberg blockade and light field dressing effect. Considering both of the primary and secondary blockades, we theoretically study the MWM process in both diatomic and quadratomic systems, in which the enhancement, suppression and avoided crossing can be affected by the atomic internuclear distance or external electric field intensity. In the diatomic system, we also can eliminate the primary blockade by the dressing effect. Such investigations have potential applications in quantum computing with Rydberg atom as the carrier of qubit.