Discovery and characterization of the novel conotoxin Lv1d from Conus lividus that presents analgesic activity.

Cone snails are predatory gastropod mollusks that are distributed in all tropical marine environments and contain small peptides (conotoxins) in their venom to capture prey. However, the biochemical and molecular aspects of conotoxins remain poorly understood. In this article, a novel α4/7-conotoxin, Lv1d, was obtained from the venom duct cDNA library of the worm-hunting Conus lividus collected from the South China Sea. The cDNA of Lv1c encodes a 65 residue conopeptide precursor, which consists of a 21 residue signal peptide, a 27 residue Pro region, and 17 residues of mature peptide. The mature peptide Lv1d was chemically synthesized according to the sequence GCCSDPPCRHKHQDLCG. It was found that 10 µM Lv1d can completely inhibit frog sciatic nerve-gastrocnemius muscle contractility within 60 min. Moreover, 100 µg/kg Lv1d showed good analgesic effects in mouse hot plate model and formalin test. Patch clamp experiments showed that 5 µM Lv1d can inhibit the cholinergic microexcitatory postsynaptic currents (mEPSCs) requency and amplitude of projection neurons in Drosophila. In conclusion, the synthesis of Lv1d and its biological and physiological data might contribute to the development of this peptide as a novel potential drug for therapeutic applications. This finding also expands the knowledge of the targeting mechanism of the α4/7-subfamily conotoxins.

Translation and Validation of a Chinese Version of the Mindfulness in Parenting Questionnaire (MIPQ).

OBJECTIVE: To translate the Mindfulness in Parenting Questionnaire (MIPQ) into Standard Mandarin and then explore the reliability and validity of this newly translated measure in a large sample of Chinese middle school parents. METHODS: We translated the MIPQ using the forward-backward method and pilot tested it on a sample of parents of adolescents (aged 12-16 years) in China. Following minor modifications, 1057 Chinese parents (Mothers or Fathers) in two middle schools (one in the North and one in the South of China) completed the translated Chinese Mindfulness in Parenting Questionnaire (C-MIPQ). To determine test-retest reliability 121 participants completed the C-MIPQ again 2 weeks later. In order to test convergent validity, 395 participants completed the Mindful Attention and Awareness Scale (MAAS), Parenting Styles and Dimensions Questionnaire-Short Version (PSDQ-short) and the Interpersonal Mindfulness in Parenting Scale (IM-P). The Chinese Perceived Stress Scale (CPSS), Beck Depression Scale (BDI-13), and socioeconomic status (SES) were completed in order to test discriminant validity. RESULTS: Confirmatory factor analysis showed that the two-factor model indicated in the original study was a good fit. The total score of the scale and the scores of the two dimensions ('Mindful discipline' and 'Being in the moment with the child') were significantly positively correlated with the total score of the MAAS, IM-P and the authoritative parenting style in the PSDQ-short, demonstrating convergent validity. Discriminant validity was established as there was no difference in C-MIPQ sub-scale scores across any of the SES variables except for levels of education (parents with higher education had higher scores on the 'being in the moment with the child' subscale). In addition, the C-MIPQ was negatively related to stress and depression. Cronbach's alpha of the total scale was 0.93 (and 0.88, 0.89 for the two dimensions of the C-MIPQ) indicating excellent internal consistency. Test-retest reliability was good (intra-class correlation of 0.83). CONCLUSION: This study is the first step toward establishing the psychometric properties of the C-MIPQ for measuring mindful parenting in parents of adolescents aged 12-16 years; additional studies will be needed in order to test this further.