ABSTRACT
The flower of Hylocereus undatus (Haw.) Britton et Rose is widely recognized as a kind of medicine-food homologous resource due to its high nutritional value. However, there is a lack of in-depth studies on the purification, structure, antioxidative and immunoregulatory activities of polysaccharides from H. undatus flowers (FHRP). The objective of this study was to investigate the primary structure, antioxidative and immunoregulatory activities of the polysaccharides extracted from Hylocereus undatus flower using water extraction and chromatogram purification. Three polysaccharide fractions named FHRP-1, FHRP-2 and FHRP-3 were obtained. The results showed that FHRP-1, FHRP-2 and FHRP-3 (200-800 µg/mL) treatment for 24 h significantly increased the activities of superoxide dismutase (SOD) and catalase (CAT), and reduced the malondialdehyde (MDA) production in RAW 246.7 cells under H2O2-induced oxidative stress. Additionally, all three fractions exhibited immunoregulatory activities by enhancing the pinocytosis of RAW 264.7 cells and promoting the production of nitric oxide (NO), interleukin 6 (IL-6), and tumor necrosis factor α (TNF-α). Among three polysaccharide fractions, FHRP-3 exhibited the most promising antioxidative and immunoregulatory properties, which was attributed to its higher content of uronic acid, moderate molecular weight, and triple-helix conformation. These findings provide preliminary insights into the primary structural information and biological activities of FHRP.
