ABSTRACT
Two isomeric dinuclear Cerium(II) complexes 1 and 2, formulated as Ce2(phen)2(NO3)2(L)4 [L=phenylacetic acid, phen=1,10-phenanthroline] was synthesized under solvothermal conditions at different pH values. The two complexes were characterized by elemental analysis, IR and single crystal X-ray diffraction. Complexes 1 and 2 were studied the binding with DNA and against cytotoxic activity. Fluorescence analysis indicated that the two complexes can bind to DNA. The changes with different gradient concentration of DNA added into the complexes in absorption spectra show a strongπ-stacking interaction between the complexes and DNA base pairs. The Cerium(II) complexes showed good cytotoxic activity against cancer cell lines, being 2 the most potent complex. Apoptotic studies of the two novel dinuclear complexes showed significant inhibitory rate on cancer cell growth line KB.
Subject(s)
Antineoplastic Agents/chemistry , Cerium/chemistry , Coordination Complexes/chemical synthesis , DNA/chemistry , Antineoplastic Agents/metabolism , Antineoplastic Agents/toxicity , Apoptosis/drug effects , Cell Line, Tumor , Coordination Complexes/chemistry , Coordination Complexes/metabolism , Coordination Complexes/toxicity , Crystallography, X-Ray , DNA/metabolism , HeLa Cells , Humans , Hydrogen Bonding , Hydrogen-Ion Concentration , Isomerism , Molecular Conformation , Phenanthrolines/chemistry , Spectrophotometry, UltravioletABSTRACT
Two new zinc complexes, namely Zn(L(1))ClCH2NO(1) and {Zn(L(2))CH2NO}nâªN(CH3)3âªClO4(2) (L(1) = 3,5-di(1H-imidazol-1-yl)pyridine L(2) = 1,3,5-tris(1-imidazolyl) benzene), have been synthesized, and characterized by IR spectra, elemental analysis, and a single crystal X-ray diffraction. Fluorescence spectroscopy indicated that two complexes presented strong DNA binding affinity constants to fish sperm DNA (FS-DNA). Gel electrophoresis assay demonstrated the ability of the complex to cleave the HL-60 DNA. Apoptotic study showed the complex exhibited significant cancer cell(KB) inhibitory rate.
Subject(s)
Antineoplastic Agents/chemical synthesis , Antineoplastic Agents/pharmacology , Coordination Complexes/chemical synthesis , Coordination Complexes/pharmacology , Imidazoles/chemistry , Zinc/chemistry , Antineoplastic Agents/chemistry , Antineoplastic Agents/metabolism , Apoptosis/drug effects , Chemistry Techniques, Synthetic , Coordination Complexes/chemistry , Coordination Complexes/metabolism , DNA/metabolism , Humans , KB Cells , Ligands , Spectrometry, FluorescenceABSTRACT
The new complex of {[Co(4,4'-Bipy)(H2O)4]·(Pyri) · 3H2O}n (4,4'-Bipy = 4,4'-bipyridyl, H2Pyri = 3,5- Pyridinedicarboxylic acid) was synthesized and characterized by IR, element analysis and X-ray single-crystal diffraction. The binding of the complex with extracted HeLa cells DNA was investigated by UV and fluorescence spectrum. Gel electrophoresis assay demonstrated the ability of the complex cleaving the extracted HC-DNA. The complex exhibited a higher cytotoxicity against tumor cells in vitro. Furthermore, the apoptotic tests indicated the complex had an apoptotic effect on HeLa cells.
