Defective silicotungstic acid-loaded magnetic floral N-doped carbon microspheres for ultra-fast oxidative desulfurization of high sulfur liquid fuels.

Highly active Keggin-type silicotungstic acid (SiW12) with oxygen vacancy (Ov) defects was encapsulated into the magnetic floral N-doped carbon microspheres (γ-Fe2O3@NC-300) through the facile one-step air pyrolysis of the precursor comprising core-shell Fe3O4@polydopamine (Fe3O4@PDA) and SiW12 to prepare γ-Fe2O3@NC@SiW12-300. The fabricated catalysts were systematically characterized and subsequently employed for the oxidation desulfurization (ODS) of the model fuel. The magnetic floral γ-Fe2O3@NC@SiW12-300 catalyst exhibited nearly perfect catalytic activity, which under mild conditions could remove 100% amount of 4000 ppm DBT in model fuel within 20 min (0.03 g catalysts and n(H2O2)/n(S) of 2). The catalyst activity is mainly attributed to the high activity SiW12 with the Ov defect and its outstanding dispersibility in γ-Fe2O3@NC, along with the high number of exposed active sites. A selected catalyst, γ-Fe2O3@NC@SiW12-300, showed a noticeable turnover frequency (TOF) (110.07 h-1) and lower activation energy (38.79 kJ mol-1) in oxidative desulfurization (ODS) with good recyclability. HO˙ radical was found to be the active species involved in ODS as confirmed by the EPR and scavenger experiments. Additionally, the fabricated catalyst can be conveniently separated and recycled within an externally applied magnetic field.

In Vivo and In Vitro Analyses of Titanium-Hydroxyapatite Functionally Graded Material for Dental Implants.

PURPOSE: The stress shielding effect caused due to the mechanical mismatch between the solid titanium and the surrounding bone tissue warrants the utilization of a mechanically and biologically compatible material such as the titanium-hydroxyapatite (Ti-HA) functionally graded material (FGM) for dental implants. This study is aimed at fabricating a Ti-HA FGM with superior mechanical and biological properties for dental implantation. MATERIALS AND METHODS: We fabricated a Ti-HA FGM with different Ti volume fractions (VFs) using HA and Ti powders. Ti-HA was characterized by studying its mechanical properties. Cytotoxicity was examined using a Cell Counting Kit-8 assay and an LDH cell cytotoxicity assay. Scanning electron microscopy was performed on an XL30 environmental scanning electron microscope (ESEM). Alkaline phosphatase (ALP) and transforming growth factor (TGF-ß1) expressions were quantitatively monitored using enzyme-linked immunosorbent assay (ELISA) kits. The expressions of TGF-ß receptors and ALP genes were measured using real-time polymerase chain reaction. The Ti-HA FGM dental implants were placed in beagle dogs. Microcomputed tomography (CT) and hard tissue slices were performed to evaluate the bone-implant contact (BIC) and bone volume over total volume (BV/TV). RESULTS: The density and mechanical properties of the Ti-HA exhibited various graded distributions corresponding to VF. Based on the results of the Cell Counting Kit-8 (CCK-8) and lactate dehydrogenase (LDH) assays, the difference in cytotoxicity between the two groups was statistically nonsignificant (P = 0.11). The ALP and TGF-ß1 levels were slightly upregulated. The transcript levels of ALP and TGF-ßRI were higher in the Ti-HA groups than in the Ti group at 7 days, whereas the transcript levels of TGF-ßRII exhibited no obvious increase. The BIC did not exhibit significant differences between the Ti and Ti-HA FGM groups (P = 0.0504). BV/TV showed the Ti-HA FGM group had better osteogenesis (P = 0.04). CONCLUSION: Ti-HA FGM contributes to the osteogenesis of dental implants in vivo and in vitro.

Role of tribochemistry reactions of B4C on tribofilm growth at a PEEK-steel interface in simulated body fluids.

Corrosion and wear products of metallic implants can lead to severe adverse tissue reactions. However, there is an absence of effective means to reduce the tribocorrosion of metal. The main purpose of this study is to reveal a mechanism of engineering a barrier layer on metal surfaces via adding functional particles into the polymer counterpart. B4C and BN particles were compounded into a polyetheretherketone (PEEK) matrix and their tribological performance of PEEK-based composites sliding against stainless steel was compared in simulated body fluid. Results demonstrate that the addition of B4C reduces significantly friction and wear. In particular, the addition of only 1 vol% B4C reduces wear of PEEK by up to 94.8%, and tribocorrosion of steel is also obviously mitigated. It is discovered that hydrolysis of B4C particles triggered by friction and deposition of Ca2+ and phosphate ions dominate formation of the barrier layer at the friction interface. The barrier layer endows the PEEK-metal sliding pair simultaneously enhanced anti-wear and anti-corrosion performance.

Tuning the tribofilm nanostructures of polymer-on-metal joint replacements for simultaneously enhancing anti-wear performance and corrosion resistance.

Total joint replacement is currently the most successful clinical treatment for improving the life quality of individuals afflicted with end-stage osteoarthritis of knee or hip joints. However, release of wear and corrosion products from the prostheses is a critical issue causing adverse physiological responses of local issues. ß-SiC nanoparticles were dispersed into polyetheretherketone (PEEK) materials and their role in tribocorrosion performance of PEEK-steel joints exposed to simulated body fluid was investigated. It is demonstrated that ß-SiC nanoparticles increase greatly the wear resistance of the PEEK materials, and meanwhile mitigate significantly corrosion of the steel counterpart. It is revealed that tribochemical reactions of ß-SiC nanoparticles promoted formation of a robust tribofilm having complex structures providing protection and shielding effects. The present work proposes a strategy for developing high-performance polymer-on-metal joint replacement materials of enhanced lifespan and biocompatibility via tuning interface nanostructures. STATEMENT OF SIGNIFICANCE: Adverse tissue responses to metal wear and corrosion products from metal base implants remain a challenge to surgeons and patients. We demonstrated that leaching of metal ions and release of metallic debris are well decreased via tuning interface nanostructures of metal-polymer joint bearings by dispersing ß-SiC nanoparticles into polyetheretherketone (PEEK). It is identified that the addition of ß-SiC greatly improves the tribological performances of the PEEK materials and mitigated corrosion of the steel. Tribo-chemistry reactions of SiC induce the formation of complex structures which provide protection and shielding effects. Nanostructures of the tribofilm were also comprehensively investigated. These novel findings proposed a potential route for designing high performance metal-polymer joint replacement materials.

Adenovirus-mediated expression of keratinocyte growth factor promotes secondary flap necrotic wound healing in an extended animal model.

BACKGROUND: No effective treatments have been found for flap necrosis. Animal models that focus on the initial flap viability are inappropriate for necrotic wound studies. Keratinocyte growth factor (KGF) promotes keratinocyte proliferation with stronger activity and fewer complications and thus may be useful for necrotic flap wound healing. METHODS: Rats with modified flap necrosis were randomly divided into four groups. An adenoviral vector expressing KGF was injected subdermally in the back of the animals after necrosis began. The expression and effect of KGF was assessed by real-time polymerase chain reaction, enzyme-linked immunoassay, and transwell, and wound healing was monitored. RESULTS: The plasmid and adenovirus were able to express KGF and stimulate epithelial cell growth (p = 0.029). Histology showed that the necrosis healed fastest in the KGF administration group than in the control groups (p < 0.01). The adenovirus-mediated KGF (Ad-KGF) group had the thickest epithelium on days 15 (p = 0.044) and 25 (p = 0.014). The KGF level in the blood serum soared 10 and 15 days postoperatively (p < 0.01) but returned to baseline by day 25 (p = 0.561). The KGF mRNA levels in vivo increased dramatically in the Ad-KGF group (p = 0.037). CONCLUSIONS: The extended flap model is applicable in necrotic wound study. Keratinocyte growth factor can promote secondary necrotic flap wound healing, and administration of KGF can be achieved by an adenoviral vector.