Molecular characterization and ontogenetic expression profiles of LPXRFa and its receptor in Japanese flounder (Paralichthys olivaceus).

Investigations concerning the LPXRFa system are rarely conducted in flatfish species. Here, we first identified and characterized lpxrfa and its cognate receptor lpxrfa-r genes in the Japanese flounder (Paralichthys olivaceus). The coding DNA sequence of lpxrfa was 579 bp in length, wich encoded a 192-aa preprohormone that can produce three mature LPXRFa peptides. The open reading frame (ORF) of lpxrfa-r was 1446 bp in size, and encoded a 481-aa LPXRFa-R protein that encompassed seven hydrophobic transmembrane domains. Subsequently, tissue distribution expression profiles of lpxrfa and lpxrfa-r transcripts were assayed by quantitative real-time PCR. The results indicated that expressions of lpxrfa transcripts were detected at the highest levels in the brain of both females and males, however, lpxrfa-r transcripts were remarkablely expressed in the brain tissue of female fish and in the testis tissue of male fish. Furthermore, transcript levels of lpxrfa and lpxrfa-r genes were investigated during early ontogenetic development, with the maximum expression levels at 30 days post-hatching. Overall, these data contribute to providing preliminary proof for the existence and structure of the LPXRFa system in Japanese flounder, and the study is just the foundation for researching physiological function of LPXRFa system in this species.

Coactosin-Like Protein in Breast Carcinoma: Friend or Foe?

Background: Coactosin-like protein (COTL1) was first identified as protein that binds 5-lipoxygenase and F-actin; its functions in tumors remain unknown. COTL1 could inhibit the proliferation of breast cancer (BRCA) in vivo and in vitro; however, online public databases including UALCAN and Kaplan-Meier plotter showed high COTL1 expression in breast cancer tissue, which was correlated with poor prognosis. Therefore, we studied the role of COTL1 expression in human breast cancer and its use in determining clinical prognosis. Methods: We first used the UALCAN database and immunohistochemical analysis to elucidate COTL1 expression in BRCA. We then performed Kaplan-Meier plotting and immunohistochemical analysis to assess prognosis in BRCA in relation to COTL1 expression. Finally, we used the CancerSEA and LinkedOmics databases to evaluate the function of COTL1 in BRCA. The TIMER and TISIDB databases were used to evaluate the association between COTL1 expression and immune cell infiltration in BRCA. Results: UALCAN and immunohistochemical analysis showed that COTL1 was highly expressed in breast cancer. Furthermore, high COTL1 expression was correlated with poor prognosis in BRCA. We also found that COTL1 is involved in immune response via the CancerSEA and LinkedOmics databases. The TIMER and TISIDB databases showed that high COTL1 expression was correlated with immune cell infiltration. Conclusion: COTL1 expression was higher in breast cancer tissues than in normal tissues, and high COTL1 expression was correlated with poor prognosis and immune cell infiltration. These results provide a basis for the development of applications of COTL1 in determining the prognosis of breast cancer and its treatment.