A Compact Broadband Power Combiner for High-Power, Continuous-Wave Applications.

A compact broadband combiner with a high power capacity and a low insertion loss, which is especially useful for solid-state power sources where multi-way power synthesis is needed, was designed and experimentally investigated. The combiner could combine the microwave signals of sixteen terminals into a single one and was based on a radial-line waveguide whose circumferential symmetry benefited the amplitude and phase consistency of the combiner. Simulation and experimental results showed that the prototype device, designed for S-band applications, exhibited a reflection coefficient S1,1 < -20 dB in the range of 2.06-2.93 GHz, which corresponds to a relative bandwidth of approximately 34.6%. At 2.45 GHz, the phase imbalance was ±4.5° and the 16-way transmission coefficient was concentrated around -12.0~-12.3 dB. The insertion loss of the device at ambient and elevated temperatures was simulated and experimentally verified, which is of importance for the design of similar high-power microwave combiners. High-power tests proved that even without enforced wind or liquid cooling, the device can handle continuous power (CW) of at least 3.9 kW, which can be much enhanced by taking regular cooling measures. The combined features of the designed combiner suggest promising applications for power synthesis in high-power, solid-state RF sources.

In Situ Combustion Synthesis of Gr/h-BN Composites and Its Passive Heat Dissipation Application.

To enhance the infrared radiation efficiency and the heat transfer performance simultaneously, graphene (Gr) was synthesized in situ on hexagonal boron nitride (h-BN) to prepare Gr/h-BN composites by a scalable combustion synthesis in CO2 atmosphere using Mg as sacrificial solder. The synthesized Gr/h-BN composites were added in polydimethylsiloxane polymer to prepare composite coatings, which show an infrared emissivity greater than 0.95 and a through-plane thermal conductivity up to 2.584 W·m-1·K-1. When functioning on an Al heatsink, such a composite coating can reduce the temperature by as much as 21.7 °C. Meanwhile, the composite coating exhibits superior adhesion on the Al substrate. Therefore, Gr/h-BN composite coatings with noteworthy infrared radiation and thermal conductivity are expected to be a promising candidate for heat dissipation applications.

Strong Hydration Ability of Silk Fibroin Suppresses Formation and Recrystallization of Ice Crystals During Cryopreservation.

The cryopreservation (CP) of cell/tissue is indispensable in medical science. However, the formation of ice during cooling and ice recrystallization/growth in time of thawing present significant risk of cell/tissue damage upon analysis of CP process. Herein, the natural and biocompatible silk fibroin (SF) with regular hydrophobic and hydrophilic domains, were first employed as a cryoprotectant (CPA), to the CP of human bone-derived mesenchymal stem cells (hBMSCs), which has been routinely cyropreserved for cell-based therapies. Addtion of SF can regulate the formation of ice crystals during cooling process because of its strong hydration ability in the comparation to the cryopreservation medium (CM) without SF. Moreover, the devitrification-induced recrystallization/growth of ice during the thawing process is suppressed. Most importantly, the addition of 10 mg mL-1 SF can achieve 81.28% cell viability of cryopreserved hBMSCs as similar as those with the addition of 180 mg mL-1 Ficoll 70 (commercial CPA), and the functions of the cryopreserved hBMSCs are maintained as good as that of the fresh ones. This work is not only significant for meeting the ever-increasing demand of cell therapy, but also trailblazing for designing materials in controlling ice formation and growth during the CP of other cells and tissues.

Use of Ion Exchange To Regulate the Heterogeneous Ice Nucleation Efficiency of Mica.

Heterogeneous ice nucleation (HIN) triggered by mineral surfaces typically exposed to various ions can have a significant impact on the regional atmosphere and climate. However, the dependence of HIN on the nature of the mineral surface ions is still largely unexplored due to the complexity of mineral surfaces. Because K+ on the atomically flat (001) surface of mica can be readily replaced by different cations through ion exchange, muscovite mica was selected; its simple nature provides a very straightforward system that can serve as the model for investigating the effects of mineral surface ions on HIN. Our experiments show that the surface (001) of H+-exchanged mica displays markedly higher HIN efficiencies than that of Na-/K-mica. Vibrational sum-frequency generation spectroscopy reveals that H-mica induces substantially less orientation ordering than Na-/K-mica within the contact water layer at the interface. Molecular dynamics simulations suggest that the HIN efficiency of mica depends on the positional arrangement and orientation of the interfacial water. The formation of the hexagonal ice Ih basal-type structure in the first water layer atop the mica surface facilitates HIN, which is determined by the size of the protruding ions atop the mica surface and by the surface adsorption energy. The orientational distribution is optimal for HIN when 25% of the water molecules in the first water layer atop the mica surface have one OH group pointing up and 25% have one OH group pointing down, which, in turn, is determined by the surface charge distribution.

Precise Control Over Kinetics of Molecular Assembly: Production of Particles with Tunable Sizes and Crystalline Forms.

It has been long-pursued but remains a challenge to precisely manipulate the molecular assembly process to obtain desired functional structures. Reported here is the control over the assembly of solute molecules, by a programmed recrystallization of solvent crystal grains, to form micro/nanoparticles with tunable sizes and crystalline forms. A quantitative correlation between the protocol of recrystallization temperature and the assembly kinetics results in precise control over the size of assembled particles, ranging from single-atom catalysts, pure drug nanoparticles, to sub-millimeter organic-semiconductor single crystals. The extensive regulation of the assembly rates leads to the unique and powerful capability of tuning the stacking of molecules, involving the formation of single crystals of notoriously crystallization-resistant molecules and amorphous structures of molecules with a very high propensity to crystallize, which endows it with wide-ranging applications.

Bioinspired l-Proline Oligomers for the Cryopreservation of Oocytes via Controlling Ice Growth.

Various types of cells are routinely cryopreserved in modern regenerative and cell-based medicines. For instance, the oocyte is one of the most demanding cells to be cryopreserved in genetic engineering and human-assisted reproductive technology (ART). However, the usage of cryopreserved oocytes in ART clinics is still limited mainly because of the unstable survival rate. This is due to the fact that oocytes are more prone to be damaged by ice crystals in comparison to other cells, as oocytes are larger in size and surface area. Meanwhile, oocytes contain more water, and thus, ice crystals are easier to form inside the cells. Currently, to avoid injury by the formed ice crystals, cryopreservation (CP) of oocytes has to use large amounts of small molecules as cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG), which can permeate into the cell and prevent ice formation inside. However, these molecules are chemically and epigenetically toxic to cells. Therefore, great efforts have been focused on reducing the amount of DMSO and EG used for oocyte CP. In nature, the antifreeze (glyco)proteins (AFGPs) locate extracellularly with the ability to protect living organisms from freezing damage via controlling ice growth. Inspired by this, biocompatible and nontoxic L-proline oligomers (L-Pron), which have the same polyproline II helix structure as that of AFGPs, are first employed for the CP of oocytes. The experimental results reveal that L-Pro8 has a profound activity in inhibiting ice growth as that of AFGP8. Also, by the addition of 50 mM L-Pro8, the amount of DMSO and EG can be greatly reduced by ca. 1.8 M for oocyte CP; moreover, the survival rate of the cryopreserved oocytes is increased up to 99.11%, and the coefficient of variance of the survival rate is decreased from 7.47 to 2.15%. These results mean that almost all oocytes can survive after CP with our method; importantly, the mitochondrial function as a critical criterion for the quality of the frozen-thawed oocytes is also improved. It is proposed that with the addition of L-Pro8, the extracellular ice growth is slowed down, which prevents the direct injuries of cells by large ice crystals and the accompanying osmotic pressure increase. As such, this work is not only significant for meeting the ever-increasing demand by the ART clinics but also gives guidance for designing materials in controlling ice growth during CP of other cells and tissues.

Unraveling Molecular Mechanism on Dilute Surfactant Solution Controlled Ice Recrystallization.

Ice recrystallization (IR) is ubiquitous, playing an important role in many areas of science, such as cryobiology, food science, and atmospheric physics. However, controllable ice recrystallization remains a challenging task largely due to an incomplete understanding of the physical mechanism associated with ice recrystallization. Herein, we explore the molecular mechanism underlying the controlling of ice recrystallization by using different small amphiphilic molecules (surfactants) through joint experimental measurements and molecular dynamics simulation. Our experiment shows that in nonionic/zwitterionic surfactant solutions, the mean size of the recrystallized ice grains increases monotonically with the concentration of surfactants, whereas in the ionic surfactant solutions, the mean size of the recrystallized ice grains tends to increase first and then decrease with increasing the concentration, yielding a peak typically at ∼5 µM. Further sequential ice affinity purification experiments and molecular dynamics simulations show that the surfactants actually do not bind to ice directly. Rather, the different spatial distributions of counter ions and molecular surfactants in the interfacial regions (ice-water interface and water-air interface) and bulk region can markedly affect the mean size of the recrystallized ice grain.

Unusual Response of Thin LiTaO3 Films to Intense Microwave Pulses.

Fundamentally different responses of a LiTaO 3 thin film detector are observed when it is subjected to short microwave pulses as the pulse intensity is altered over a wide range. We start from weak microwave pulses which lead to only trivial pyroelectric peak response. However, when the microwave pulses become intense, the normally expected pyroelectric signal seems to be suppressed and the sign of the voltage signal can even be completely changed. Analysis indicates that while the traditional pyroelectric model, which is a linear model and works fine for our data in the small regime, it does not work anymore in the large signal regime. Since the small-signal model is the key foundation of electromagnetic-wave sensors based on pyroelectric effects, such as pyroelectric infrared detecters, the observation in this work suggests that one should be cautious when using these devices in intense fields. In addition, the evolution of detector signal with respect to excitation strength suggests that the main polarisation process is changed in the large signal regime. This is of fundamental importance to the understanding on how crystalline solids interact with intense microwaves. Possible causes of the nonlinear behaviour is discussed.

Bioinspired Ice Growth Inhibitors Based on Self-Assembling Peptides.

Antifreeze proteins (AFPs) are widely found in organisms living in subzero environments. Their strong ability to inhibit ice growth and recrystallization have inspired considerable bioinspired efforts to engineer artificial ice growth inhibitors for cryopreservation. However, it remains challenging to engineer biocompatible and cost-effective synthetic ice growth inhibitors to meet the increasing needs of cryoprotectants in biomedical research and industry. Here we report the design of artificial ice growth inhibitors based on self-assembling peptides. We demonstrate the importance of threonine residues as well as their spatial arrangement for effective ice binding. The engineered self-assembling ice growth inhibiting peptides show moderate ice inhibiting activity including suppression of ice growth rates and retardation of recrystallization of ice crystals. The applications of these peptides in cryopreservation of enzymes and cells were also demonstrated.

Tuning Ice Nucleation and Propagation with Counterions on Multilayer Hydrogels.

Ice formation on solid surfaces includes heterogeneous ice nucleation and ice propagation processes. However, no study has been focused on tuning of both ice nucleation and ice propagation via a simple anti-icing coating method. In this work, we have prepared multilayer hydrogels based on simple layer-by-layer (LBL) deposition approach and discover the ion-specific effect on both ice nucleation and ice propagation. A large ice nucleation temperature window of 11 °C is controlled via changing different counterions; meanwhile, the differences in ice propagation time can be tuned up to 4 orders of magnitude. Through synergistically controlling of ice nucleation and propagation delay times, we can tune the freezing delay time of water droplets on multilayer hydrogel surfaces up to 3 orders of magnitude via changing various counterions. Considering the application requirements, these multilayer hydrogels are stable under different conditions and can be coated on various materials without destroying the existing surface. This new insight can inspire the design of anti-icing surfaces based on regulating both ice nucleation and ice propagation.

Strong peak in Tc of Sr2RuO4 under uniaxial pressure.

Sr2RuO4 is an unconventional superconductor that has attracted widespread study because of its high purity and the possibility that its superconducting order parameter has odd parity. We study the dependence of its superconductivity on anisotropic strain. Applying uniaxial pressures of up to ~1 gigapascals along a 〈100〉 direction (a axis) of the crystal lattice results in the transition temperature (Tc) increasing from 1.5 kelvin in the unstrained material to 3.4 kelvin at compression by ≈0.6%, and then falling steeply. Calculations give evidence that the observed maximum Tc occurs at or near a Lifshitz transition when the Fermi level passes through a Van Hove singularity, and open the possibility that the highly strained, Tc = 3.4 K Sr2RuO4 has an even-parity, rather than an odd-parity, order parameter.

Engineering a functional 1-deoxy-D-xylulose 5-phosphate (DXP) pathway in Saccharomyces cerevisiae.

Isoprenoids are used in many commercial applications and much work has gone into engineering microbial hosts for their production. Isoprenoids are produced either from acetyl-CoA via the mevalonate pathway or from pyruvate and glyceraldehyde 3-phosphate via the 1-deoxy-D-xylulose 5-phosphate (DXP) pathway. Saccharomyces cerevisiae exclusively utilizes the mevalonate pathway to synthesize native isoprenoids and in fact the alternative DXP pathway has never been found or successfully reconstructed in the eukaryotic cytosol. There are, however, several advantages to isoprenoid synthesis via the DXP pathway, such as a higher theoretical yield, and it has long been a goal to transplant the pathway into yeast. In this work, we investigate and address barriers to DXP pathway functionality in S. cerevisiae using a combination of synthetic biology, biochemistry and metabolomics. We report, for the first time, functional expression of the DXP pathway in S. cerevisiae. Under low aeration conditions, an engineered strain relying solely on the DXP pathway for isoprenoid biosynthesis achieved an endpoint biomass 80% of that of the same strain using the mevalonate pathway.

Mutual Effects of Glycerol and Inorganic Salts on Their Hydration Abilities.

It is a tough challenge to understand the mutual interactions among various components in aqueous solutions of inorganic mixed with organic solutes. The hydration number, nh, and critical hydration number, ncr, determined by the measurements of glass transition of the solutions, in conjunction with tracing the change in local water structure, can provide some insights into the complicated interplays in such a mixture. Here, the nh and ncr for aqueous solutions of glycerol, various chlorides, and mixtures of glycerol with a chloride are determined. The ratio of ncr/nh measures 4 for glycerol and 1.7 for all the chlorides, and for mixtures of glycerol with all of the chlorides except ZnCl2, it falls within these two extremes. Glycerol content dependence of nh and ncr reveals a rich and interesting scenario of mutual effects therein, in particular, the glycerol's replacement and sharing of hydration water with salt. In the case of ZnCl2, at most, one hydration water molecule is replaced by glycerol, and the excess glycerol molecules continuously reduce the number of glycerol molecules that share hydration water with ZnCl2. Our results can help establish a pathway for the investigation of interactions among the organic and inorganic components in aqueous solutions, which is desirable for many applications.

Rewriting yeast central carbon metabolism for industrial isoprenoid production.

A bio-based economy has the potential to provide sustainable substitutes for petroleum-based products and new chemical building blocks for advanced materials. We previously engineered Saccharomyces cerevisiae for industrial production of the isoprenoid artemisinic acid for use in antimalarial treatments. Adapting these strains for biosynthesis of other isoprenoids such as ß-farnesene (C15H24), a plant sesquiterpene with versatile industrial applications, is straightforward. However, S. cerevisiae uses a chemically inefficient pathway for isoprenoid biosynthesis, resulting in yield and productivity limitations incompatible with commodity-scale production. Here we use four non-native metabolic reactions to rewire central carbon metabolism in S. cerevisiae, enabling biosynthesis of cytosolic acetyl coenzyme A (acetyl-CoA, the two-carbon isoprenoid precursor) with a reduced ATP requirement, reduced loss of carbon to CO2-emitting reactions, and improved pathway redox balance. We show that strains with rewired central metabolism can devote an identical quantity of sugar to farnesene production as control strains, yet produce 25% more farnesene with that sugar while requiring 75% less oxygen. These changes lower feedstock costs and dramatically increase productivity in industrial fermentations which are by necessity oxygen-constrained. Despite altering key regulatory nodes, engineered strains grow robustly under taxing industrial conditions, maintaining stable yield for two weeks in broth that reaches >15% farnesene by volume. This illustrates that rewiring yeast central metabolism is a viable strategy for cost-effective, large-scale production of acetyl-CoA-derived molecules.

Design and experiment study of compact circular-rectangular waveguide mode converter.

A compact mode converter that transforms TM01 circular waveguide mode to TE10 rectangular waveguide mode is investigated. It consists of a circular waveguide with a short circuit terminal and a rectangular waveguide which is perpendicular to the circular waveguide. Simulation results show that conversion efficiency of the mode converter is about 99.8% at central frequency of 1.75 GHz, and the total return loss is approximately -30 dB. The experimental results are well consistent with the computer simulation, which demonstrates the feasibility and high power handling capacity of the mode converter.

The decisive role of free water in determining homogenous ice nucleation behavior of aqueous solutions.

It is a challenging issue to quantitatively characterize how the solute and pressure affect the homogeneous ice nucleation in a supercooled solution. By measuring the glass transition behavior of solutions, a universal feature of water-content dependence of glass transition temperature is recognized, which can be used to quantify hydration water in solutions. The amount of free water can then be determined for water-rich solutions, whose mass fraction, Xf, is found to serve as a universal relevant parameter for characterizing the homogeneous ice nucleation temperature, the meting temperature of primary ice, and even the water activity of solutions of electrolytes and smaller organic molecules. Moreover, the effects of hydrated solute and pressure on ice nucleation is comparable, and the pressure, when properly scaled, can be incorporated into the universal parameter Xf. These results help establish the decisive role of free water in determining ice nucleation and other relevant properties of aqueous solutions.

Glass transition of aqueous solutions involving annealing-induced ice recrystallization resolves liquid-liquid transition puzzle of water.

Liquid-liquid transition of water is an important concept in condensed-matter physics. Recently, it was claimed to have been confirmed in aqueous solutions based on annealing-induced upshift of glass-liquid transition temperature, T(g) . Here we report a universal water-content, X(aqu) , dependence of T(g) for aqueous solutions. Solutions with X(aqu)>X(cr)(aqu)vitrify/devitrify at a constant temperature, ~T(g) , referring to freeze-concentrated phase with X(aqu)left behind ice crystallization. Those solutions with X(aqu)

Methylerythritol phosphate pathway of isoprenoid biosynthesis.

Isoprenoids are a class of natural products with more than 55,000 members. All isoprenoids are constructed from two precursors, isopentenyl diphosphate and its isomer dimethylallyl diphosphate. Two of the most important discoveries in isoprenoid biosynthetic studies in recent years are the elucidation of a second isoprenoid biosynthetic pathway [the methylerythritol phosphate (MEP) pathway] and a modified mevalonic acid (MVA) pathway. In this review, we summarize mechanistic insights on the MEP pathway enzymes. Because many isoprenoids have important biological activities, the need to produce them in sufficient quantities for downstream research efforts or commercial application is apparent. Recent advances in both MVA and MEP pathway-based synthetic biology are also illustrated by reviewing the landmark work of artemisinic acid and taxadien-5α-ol production through microbial fermentations.

[Preparation of submicron emulsion of fresh Zhongjiefeng volatile oil].

OBJECTIVE: To prepare the submicron emulsion of fresh Zhongjiefeng volatile oil. METHOD: The Zhongjiefeng volatile oil submicron emulsion was obtained after passing the elementary emulsion through a high pressure homogenizer. The physical and chemical stability of the emulsion was evaluated with the stability parameter of centrifugation, appearance of emulsion and the pH. The formulation and processing factors were optimized by single factor reviewing and orthogonal experimental design. RESULT: By controlling various processing factors and optimizing formulation, the stable submicron emulsion of Zhongjiefeng volatile oil was prepared. Its mean particle diameter was 164-169 nm with PDI 0.084-0.107 and Zeta electric potential was -40 mV. CONCLUSION: The formulation and preparation technique of the emulsion is reasonable.