Optimization of Ultrasonic Flavonoid Extraction from Saussurea involucrate, and the Ability of Flavonoids to Block Melanin Deposition in Human Melanocytes.

(1) Background: Flavonoids are the primary medicinal ingredient of Saussurea involucrate, which have significant antioxidant capacity. Optimizing the extraction of Saussurea involucrate flavonoids (SIFs) and exploring the ability to block melanin deposition caused by reactive oxygen can greatly promote the development of S. involucrate whitening products. (2) Methods: Ultrasonic extraction process was optimized using the Box-Behnken design (BBD) and response surface methodology (RSM). Then, the effect of SIFs on antioxidant activity and anti-deposition of melanin, and genes related to the melanin synthesis are studied. (3) Results: The optimal extraction procedures are as follows: the extraction time, ethanol content, and solvent ratio (v/w) are 64 min, 54%, and 54:1, respectively. The reducing activity and scavenging rates of 2,2-diphenyl-1-picrylhydrazyl (DPPH), superoxide anion, hydroxyl radical, and ABTS+ were promoted as more S. involucrate flavonoid extract was added. The SIFs extract induced a decrease in the melanin synthesis by inhibiting the human melanoma A375 cell tyrosinase activity. SIFs also depress expression of melanin synthesis related genes. (4) Conclusions: the highest SIFs content was obtained by using 54% ethanol and 54:1 solvent ratio (v/w) for 64 min. The extract of SIFs exhibited good ability of antioxidant and anti-deposition of melanin in human melanocytes.

Coupling effects in single-mode and multimode resonator-coupled system.

We have proposed a simple metal-dielectric-metal (MDM) waveguide system side-coupled with single-mode and multimode resonators. This proposed structure can achieve a typical dual plasmon-induced transparency (PIT) effect in the transmission spectra. The two PIT peaks exhibit opposite evolution tendencies with the increase in the depth of stubs. A multimode-coupled mode theory (M-CMT), confirmed by simulated results, is originally introduced to investigate the coupling effects of the proposed structure. Compared to the previous reported multichannel filters, the proposed structure includes obvious advantages, such as structural simplicity and ease of fabrication. In addition, the sensing characteristics of the proposed structure based on PIT effects are discussed numerically. The results demonstrate that the proposed structure is suitable for applications in multichannel filters, optical switches, and sensors.

Absorption and slow-light analysis based on tunable plasmon-induced transparency in patterned graphene metamaterial.

We propose a novel simple patterned monolayer graphene metamaterial structure based on tunable terahertz plasmon-induced transparency (PIT). Destructive interference in this structure causes pronounced PIT phenomenon, and the PIT response can be dynamically controlled by voltage since the existence of continuous graphene bands in the structural design. The theoretical transmission of this structure is calculated by coupled mode theory (CMT), and the results are highly consistent with the simulation curve. After that, the influence of the graphene mobility on the PIT response and absorption characteristics is researched. It is found that the absorption efficiency of our designed structure can reach up to 50%, meaning the structure is competent to prominent terahertz absorber. Moreover, the slow-light performance of this structure is discussed via analyzing the group refractive index and phase shift. It shows that the structure possesses a broad group refractive index band with ultra-high value, and the value is up to 382. This work will diversify the designs for versatile tunable terahertz devices and micro-nano slow-light devices.

Dual plasmonically tunable slow light based on plasmon-induced transparency in planar graphene ribbon metamaterials.

We propose a simulated terahertz design based on planar graphene ribbons. With numerical simulation, we can achieve a very obvious dual plasmon-induced transparency phenomenon through the destructive interference in this structure. Moreover, due to the simple design of this structure and the complete continuous graphene ribbons, the Fermi level of graphene can be regulated by voltage. Thus, the dual plasmon-induced transparency phenomenon can be easily tuned in the numerical simulation. Further structural analysis shows that the two graphene chips on the side of the graphene ribbons play a crucial role in the dual plasmon-induced transparency phenomenon. As the length of the two chips is close, the dual plasmon-induced transparency phenomenon gradually becomes a single plasmon-induced transparency phenomenon. The theoretical analysis of this structure shows that this system has a very high group index, and its maximum value is 800, which is far greater than that of other types of slow light devices. This work may open up a new way for designing tunable terahertz graphene-based devices and slow light devices.

Dual tunable plasmon-induced transparency based on silicon-air grating coupled graphene structure in terahertz metamaterial.

A graphene plasmonic structure consists of three graphene layers mingled with a silicon-air grating is proposed. We theoretically predict and numerically simulate the plasmon-induced transparency effect in this system at terahertz wavelengths, and a dual plasmon-induced transparency peaks can be successfully tuned by virtually shifting the desired Fermi energy on graphene layers. We investigate the surface plasmon dispersion relation by means of analytic calculations, and we can achieve the numerical solution of propagation constant got by the dispersion relation. A suitable theoretical model is established to study spectral features in the plasmonic graphene system, and the theoretical results agree well with the simulations. The proposed model and findings may provide guidance for fundamental research of highly tunable optoelectronic devices.

Novel oscillator model with damping factor for plasmon induced transparency in waveguide systems.

We introduce a novel two-oscillator model with damping factor to describe the plasmon induced transparency (PIT) in a bright-dark model plasmonic waveguide system. The damping factor γ in the model can be calculated from metal conductor damping factor γ c and dielectric damping factor γ d . We investigate the influence of geometry parameters and damping factor γ on transmission spectra as well as slow-light effects in the plasmonic waveguide system. We can find an obvious PIT phenomenon and realize a considerable slow-light effect in the double-cavities system. This work may provide guidance for optical switching and plasmon-based information processing.

Expression, purification and preliminary crystallographic analysis of human thyroid hormone responsive protein.

Thyroid hormone responsive protein (Thrsp, also known as Spot 14 and S14) is a carbohydrate-inducible and thyroid-hormone-inducible nuclear protein specific to liver, adipose and lactating mammary tissues. Thrsp functions to activate genes encoding fatty-acid synthesis enzymes. Recent studies have shown that in some cancers human Thrsp (hS14) localizes to the nucleus and is amplified, suggesting that it plays a role in the regulation of lipogenic enzymes during tumourigenesis. Thrsp, a member of the Spot 14 superfamily, is an acidic homodimeric protein with no sequence similarity to other mammalian gene products and its biochemical function is elusive. To shed light on the structure-function relationship of this protein, human Thrsp was crystallized. Recombinant human Thrsp (hThrsp), the N-terminally truncated human Thrsp(10-146) (hThrsp9) and their selenomethionyl (SeMet) derivatives were expressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 293 K using Li(2)SO(4) as a precipitant. Using synchrotron radiation, data for the hThrsp SeMet derivative, hThrsp9 and its SeMet derivative were collected to 4.0, 3.0 and 3.6 Šresolution, respectively, at 100 K. The crystals of full-length hThrsp and its SeMet derivative belonged to space group P4(1)2(1)2, with approximate unit-cell parameters a = b = 123.9, c = 242.1 Å, α = ß = γ = 90.0°. In contrast, the crystals of the truncated hThrsp9 and its SeMet derivative belonged to space group P2(1)2(1)2(1), with approximate unit-cell parameters a = 91.6, b = 100.8, c = 193.7 Å, α = ß = γ = 90.0°. A molecular-replacement solution calculated using a murine Spot 14 structure as a search model indicated the presence of six molecules per asymmetric unit, comprising three hThrsp homodimers.

Purification, crystallization and preliminary X-ray diffraction analysis of human Gadd45gamma.

Gadd45, MyD118 and CR6 (also termed Gadd45alpha, Gadd45beta and Gadd45gamma, respectively) comprise a family of proteins that play important roles in negative growth control, maintenance of genomic stability, DNA repair, cell-cycle control and apoptosis. Recombinant human Gadd45gamma and its selenomethionine derivative were expressed in an Escherichia coli expression system and purified; they were then crystallized using the hanging-drop vapour-diffusion method. Diffraction-quality crystals were grown at 291 K using PEG 3350 as precipitant. Using synchrotron radiation, the best diffraction data were collected to 2.3 A resolution for native crystals at 100 K; selenomethionyl derivative data were collected to 3.3 A resolution. All the crystals belonged to space group I2(1)3, with approximate unit-cell parameters a = b = c = 126 A.