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1.
Eur Rev Med Pharmacol Sci ; 24(8): 4382-4388, 2020 04.
Article in English | MEDLINE | ID: mdl-32373975

ABSTRACT

OBJECTIVE: The aim of this study was to elucidate whether FOXD2-AS1 stimulated glioma progression by inhibiting the P53 level. PATIENTS AND METHODS: FOXD2-AS1 expression in glioma tissues and cell lines was determined by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Meanwhile, FOXD2-AS1 expression in glioma patients with different tumor tissues and tumor staging was examined as well. The subcellular distribution of FOXD2-AS1 was analyzed. RNA Binding Protein Immunoprecipitation (RIP) and Chromatin immunoprecipitation (ChIP) assay were applied to explore the interaction between FOXD2-AS1 and P53. Furthermore, the influences of FOXD2-AS1 and P53 on the viability and colony formation abilities of LN229 and U87 cells were assessed. RESULTS: FOXD2-AS1 was significantly upregulated in glioma tissues and cells. The expression level of FOXD2-AS1 was positively correlated with tumor size and staging of glioma. FOXD2-AS1 was mainly distributed in the nucleus, which could attenuate recruitment ability to P53 by bounding to EZH2. The silence of FOXD2-AS1 significantly decreased the viability and colony formation abilities of glioma cells. However, the attenuated proliferative ability was partially reversed by P53 knockdown. CONCLUSIONS: FOXD2-AS1 stimulated the proliferation of glioma by inhibiting P53, thus aggravating the progression of glioma.


Subject(s)
Glioma/metabolism , Glioma/pathology , RNA, Long Noncoding/metabolism , Tumor Suppressor Protein p53/metabolism , Cell Proliferation , Cells, Cultured , Glioma/surgery , Humans , RNA, Long Noncoding/genetics , Tumor Suppressor Protein p53/genetics
2.
Eur Rev Med Pharmacol Sci ; 22(17): 5740-5746, 2018 09.
Article in English | MEDLINE | ID: mdl-30229852

ABSTRACT

OBJECTIVE: This study aims to compare clinical efficiency of mechanical thrombectomy combined with rhPro-UK thrombolysis on moderate or severe acute brain infarction. PATIENTS AND METHODS: A total of 90 acute cerebral infarction patients due to artery stenosis or blockade from May 2016 to May 2017 were recruited and randomly assigned into thrombolysis group (N = 30), mechanical thrombectomy (N = 30), and combined treatment group (N = 30). Clinical information was collected. Thrombolysis group received rhPro-UK, mechanical thrombectomy group received Solitaire scaffold, and combined group received rhPro-UK after Solitaire scaffold. Barthel scale and NIHSS scale were used to evaluate the quality of life and mental deficit of patients. Modified thrombolysis in cerebral infarction (mTICI) was compared among three groups, along with the observation of hemorrhage, neurological recovery within 90 days, and adverse effects. RESULTS: No significant difference was found in NIHSS within 24 h of treatment among three groups (p > 0.05), but the decreasing levels were shown at 24 h, 7 days, and 90 days comparing to those before treatment (p < 0.05). In combined treatment group, lower NIHSS at 7 d and 90 d were detected comparing to other two groups (p < 0.05). Recanalization rates were 53.33% and 60.00% in thrombolysis and mechanical groups (p > 0.05), respectively, which were significantly lower than that in combined group (83.33%) (p < 0.05). Curative rate in combined group was 70%, significantly higher than thrombolysis (46.67%) and mechanical group (53.33%) (p < 0.05). No statistical difference of curative rate was observed between thrombolysis and mechanical groups (p > 0.05). Moreover, neither significant difference of coagulation function nor platelet count was found among three groups (p > 0.05). CONCLUSIONS: Mechanical thrombectomy combined with thrombolysis presented favorable efficiency in the treatment of moderate to severe acute cerebral infarction than single treatment, among which the occurrence of adverse effects were similar.


Subject(s)
Cerebral Infarction/therapy , Fibrinolytic Agents/administration & dosage , Thrombectomy , Thrombolytic Therapy , Urokinase-Type Plasminogen Activator/administration & dosage , Aged , Cerebral Infarction/diagnosis , Cerebral Infarction/physiopathology , China , Combined Modality Therapy , Female , Fibrinolytic Agents/adverse effects , Humans , Male , Middle Aged , Recovery of Function , Risk Factors , Severity of Illness Index , Thrombectomy/adverse effects , Thrombectomy/instrumentation , Thrombolytic Therapy/adverse effects , Time Factors , Treatment Outcome , Urokinase-Type Plasminogen Activator/adverse effects
3.
Eur Rev Med Pharmacol Sci ; 22(6): 1693-1699, 2018 03.
Article in English | MEDLINE | ID: mdl-29630114

ABSTRACT

OBJECTIVE: Previous study reported that miR-498 served as a tumor suppressor in non-small cell lung cancer (NSCLC), but the underlying mechanism remains largely unknown. The aim of this study is to investigate the role of miR-498 and its target gene HMGA2 in NSCLC progression. PATIENTS AND METHODS: The expression of miR-498 was assessed in clinical NSCLC specimens and cell lines using RT-PCR. Overexpression of miR-498 and transfection of pLenti-HMGA2 were performed in A549 cells. Cell proliferation, apoptosis, migration, and invasion were determined using cell counting kit-8 (CCK-8) assay, clone formation assay, flow cytometry, and transwell assay, respectively. Luciferase reporter assays were performed to analyze the regulation of putative target of miR-498. Western blot was used to detect the levels of HMGA2 in A549 cells. RESULTS: MiR-498 was found to be down-regulated in NSCLC tissues and cell lines. After miR-498 mimics transfection, cell proliferation, migration, and invasion were significantly suppressed in the NSCLC cells. Mechanistically, bioinformatic analysis predicted that miR-498 may target the 3'-UTR of HMGA2 and suppressed its translation, and was further confirmed by luciferase assay. Furthermore, restoration of HMGA2 expression completely rescued the inhibitory effect of miR-498 in NSCLC cells. CONCLUSIONS: This paper revealed that miR-498 may serve as a tumor suppressor in NSCLC through targeting HMGA2, suggesting that miR-498 could represent a novel target for effective therapies.


Subject(s)
Carcinoma, Non-Small-Cell Lung/genetics , HMGA2 Protein/genetics , Lung Neoplasms/genetics , MicroRNAs/genetics , A549 Cells , Apoptosis/genetics , Carcinoma, Non-Small-Cell Lung/pathology , Cell Movement/genetics , Cell Proliferation/genetics , Down-Regulation , Female , Genes, Tumor Suppressor , Humans , Lung Neoplasms/pathology , Oncogenes , Transfection
4.
Fa Yi Xue Za Zhi ; 33(4): 388-392, 2017 Aug.
Article in Chinese | MEDLINE | ID: mdl-29219271

ABSTRACT

OBJECTIVES: To investigate the allelic distribution of 19 autosomal STR loci in Guizhou Han population, and to estimate the forensic application value. METHODS: The 19 autosomal STR loci in 520 unrelated healthy individuals from Guizhou Han population were studied using Goldeneye™ 20A kit. The 310 genetic analyzer was used for capillary electrophoresis, and the GeneMapper®ID v3.1 for genotyping. RESULTS: The heterozygosis, the discrimination power, the probability of exclusion, the polymorphism information content, the cumulative discrimination power and the cumulative probability of exclusion of the 19 STR loci were 0.603 8-0.916 4, 0.790 0-0.985 6, 0.295 5-0.826 9, 0.553 5-0.908 9, 1-1.230 0×10⁻²² and 0.999 999 99, respectively. Compared with other five Han populations in pairwise allelic frequencies, Guizhou Han only had significant differences with Shandong Han, Liaoning Han and Shanxi Han. CONCLUSIONS: The 19 autosomal STR loci such as D19S433 have a highly genetic polymorphic in Guizhou Han population, which have application values in the researches of population genetics and forensic genetics.


Subject(s)
Asian People/genetics , Chromosomes, Human, Y/genetics , Genetic Loci , Genetic Variation , Polymorphism, Genetic/genetics , Alleles , Asian People/ethnology , China/epidemiology , Ethnicity/genetics , Genetic Testing , Genetics, Population , Genotype , Heterozygote , Humans
5.
Eur Rev Med Pharmacol Sci ; 20(14): 3031-4, 2016 07.
Article in English | MEDLINE | ID: mdl-27460731

ABSTRACT

OBJECTIVE: Emerging evidence suggest that long non-coding RNAs (lncRNAs) may play important roles in human cancers. The aim of this study was to investigate the expression of SBF2-AS1 in non small cell lung cancer (NSCLC) and its correlation with clinicopathological features and prognosis in NSCLC. PATIENTS AND METHODS: The expression of lncRNA SBF2-AS1 was measured in 174 NSCLC samples and their matched non-tumor tissues by using RT-PCR. Association of SBF2-AS1 expression with clinicopathological features was analyzed in NSCLC. Kaplan-Meier analysis was performed to evaluate the overall survival of NSCLC patients. RESULTS: The expression of SBF2-AS1 was higher in NSCLC tissues compared with adjacent non-tumor tissues (p < 0.01). Additionally, high expression level of SBF2-AS1 was significantly associated with NSCLC histological grade, and lymph node metastasis. Furthermore, a higher SBF2-AS1 expression was demonstrated to be associated with poor overall survival times in NSCLC patients (p < 0.001). Multivariate analysis suggested that SBF2-AS1 expression was an independent prognostic factor for overall survival of patients with NSCLC (p = 0.013). CONCLUSIONS: Our data suggest that SBF2-AS1 could represent a novel prognostic marker and potential therapeutic target in patients with NSCLC.


Subject(s)
Carcinoma, Non-Small-Cell Lung/genetics , Lung Neoplasms/genetics , RNA, Long Noncoding/genetics , Carcinoma, Non-Small-Cell Lung/pathology , Gene Expression Regulation, Neoplastic , Humans , Kaplan-Meier Estimate , Lung Neoplasms/pathology , Prognosis
6.
Br J Anaesth ; 115(5): 752-60, 2015 Nov.
Article in English | MEDLINE | ID: mdl-26475803

ABSTRACT

BACKGROUND: We tested the hypothesis that developmental effects of repeated neonatal exposure to sevoflurane in rats are exacerbated by stressful experiences received later in life. METHODS: Sprague-Dawley male rats received sequential exposures to 3% sevoflurane for two h on postnatal days (P) six, seven, and eight. After weaning at P21, rats were housed either in pairs in an enriched environment (EE) or singly in an enrichment-deprived environment (an adverse environment, AE). The hippocampal concentrations of brain-derived neurotrophic factor (BDNF), and synaptic markers were assessed at P8 and P53. The dentate gyrus neural progenitor proliferation was evaluated at P11 and P53 after administration of bromodeoyuridine (BrdU) at P8 to P10 and at P22 to P27, respectively. Neurobehavioural evaluations were performed at P49 to P53. RESULTS: Repeated sevoflurane exposure acutely reduced concentrations of BDNF, synaptic markers and neural progenitor proliferation. The sevoflurane group housed in the AE conditions (sevoflurane+AE) had decreased concentrations of BDNF and synaptic markers, and survival of new granule cells and impaired cognitive function compared with the control+AE, control+EE, and sevoflurane+EE groups. The neurobehavioural parameters in the sevoflurane+EE and control+EE groups were similar. CONCLUSIONS: Neurocognitive abnormalities induced by repeated neonatal exposure to sevoflurane can be aggravated by stressful conditions such as social isolation and enrichment deprivation.


Subject(s)
Anesthetics, Inhalation/toxicity , Behavior, Animal/drug effects , Cognition Disorders/etiology , Methyl Ethers/toxicity , Social Isolation/psychology , Animals , Animals, Newborn , Brain-Derived Neurotrophic Factor/metabolism , Cell Proliferation/drug effects , Cognition Disorders/metabolism , Conditioning, Classical/drug effects , Dentate Gyrus/drug effects , Dentate Gyrus/pathology , Hippocampus/drug effects , Hippocampus/metabolism , Male , Neural Stem Cells/drug effects , Neural Stem Cells/pathology , Rats, Sprague-Dawley , Sevoflurane , Social Environment
7.
Acta Biol Hung ; 65(3): 294-304, 2014 Sep.
Article in English | MEDLINE | ID: mdl-25194733

ABSTRACT

Lipopolysaccharide and b-1,3-glucan binding protein (LGBP) is a pattern recognition receptor that can recognize and bind LPS and b-1,3-glucan. LGBP has crucial roles in innate immune defense against Gram-negative bacteria and fungi. In this study, LGBP functions in Portunus trituberculatus innate immunity were analyzed. First, the mRNA expression of PtLGBP in hemocytes, hepatopancreas, and muscle toward three typical pathogen-associated molecular patterns (PAMPs) stimulations were examined using real-time PCR. Results show that the overall trend of relative expressions of the LGBP gene in three tissues is consistent, showing up-down trend. In each group, the highest expression of the LGBP gene was at 3 and 12 h post-injection. The LGBP gene is also expressed significantly higher in the hemocytes and hepatopancreas than in the muscle. The highest level of LGBP was in the lipopolysaccharides (LPS) and glucan-injected group, whereas the lowest level was in the PGN-injected group. Furthermore, bacterial agglutination assay with polyclonal antibody specifically for PtLGBP proved that the recombinant PtLGBP (designated as rPtLGBP) could exhibit obvious agglutination activity toward Gram-negative bacteria Escherichia coli, Vibrio parahaemolyticus, and V. alginolyticus; Gram-positive bacteria Bacillus subtilis; and fungi Saccharomyces cerevisiae. LGBP in Portunus trituberculatus possibly served as a multi-functional PRR. In addition, LGBP is not only involved in the immune response against Gram-negative and fungi, as manifested in other invertebrates, but also has a significant role in anti-Gram-positive bacteria infection.


Subject(s)
Brachyura/drug effects , Endotoxins/pharmacology , Immunity, Innate/drug effects , Lectins/drug effects , Peptidoglycan/pharmacology , Receptors, Pattern Recognition/drug effects , beta-Glucans/pharmacology , Agglutination/drug effects , Agglutination Tests , Animals , Blotting, Western , Brachyura/genetics , Brachyura/immunology , Brachyura/metabolism , Escherichia coli , Gene Expression Regulation , Hemocytes/drug effects , Hemocytes/immunology , Hemocytes/metabolism , Hepatopancreas/drug effects , Hepatopancreas/immunology , Hepatopancreas/metabolism , Lectins/genetics , Lectins/metabolism , Muscles/drug effects , Muscles/immunology , Muscles/metabolism , RNA, Messenger/metabolism , Receptors, Pattern Recognition/genetics , Receptors, Pattern Recognition/metabolism , Saccharomyces cerevisiae , Staphylococcus aureus , Time Factors
8.
Fitoterapia ; 72(7): 832-3, 2001 Nov.
Article in English | MEDLINE | ID: mdl-11677026

ABSTRACT

The isolation from the acetone extract of Lethariella cladonioides of the new compound cladonioidesin (1) and 10 other constituents is reported.


Subject(s)
Ascomycota , Phthalic Acids/chemistry , Phytotherapy , Plant Extracts/chemistry , Humans
9.
Phytochemistry ; 58(1): 179-83, 2001 Sep.
Article in English | MEDLINE | ID: mdl-11524129

ABSTRACT

Four 7,20-epoxy ent-kaurane diterpenoids, xerophilusins G (1) and I-K (2-4), were isolated from the leaves of Isodon xerophilus, along with four known ones, enanderianin C (5), rosthorin A (6), longikaurin B (7), and rabdoternin D (8). Their structures were determined primarily using NMR spectroscopic techniques. The structure and stereochemistry of 3 were confirmed by X-ray crystallography. Compounds 4 and 7 exhibited broad cytotoxicity against four kinds of human tumor cells (K562, HL-60, HCT, and MKN-28 cells) in the range of 2.23-15.35 and 0.30-8.61 microg/ml, respectively.


Subject(s)
Antineoplastic Agents, Phytogenic/chemistry , Diterpenes, Kaurane , Diterpenes/chemistry , Lamiaceae/chemistry , Plants, Toxic/chemistry , Antineoplastic Agents, Phytogenic/isolation & purification , Antineoplastic Agents, Phytogenic/toxicity , Crystallography, X-Ray , Diterpenes/isolation & purification , Diterpenes/toxicity , Drug Screening Assays, Antitumor , Magnetic Resonance Spectroscopy , Mass Spectrometry , Models, Molecular , Molecular Conformation
10.
Zhongguo Yi Liao Qi Xie Za Zhi ; 25(1): 41-4, 2001 Jan.
Article in Chinese | MEDLINE | ID: mdl-12583303

ABSTRACT

This paper introduces a kind of all-purpose medical electronic teaching instrument. According to the modularization design rule, a medical electronic teaching instrument integrated with information transfer, signal processing, keyboard monitoring and signal sampling, etc, can be realized. On the other hand, A/D, D/A, self-detecting of computer, collecting and testing of biological potentials, driving electromotor and generating functional signals, etc can also be realized by the related software based on the system. It has been proved that, by using single-chip processor and computer, the system can implement full-scale test and processing of biomedical signals so that operators can understand the principles and the construction of medical instruments more easily and deeply. Therefore, it is valuable in application of medical electronic coursesteaching, research and etc.


Subject(s)
Computer-Assisted Instruction/instrumentation , Education, Medical , Educational Technology/instrumentation , Software , Equipment Design , Software Design
11.
Fitoterapia ; 71(4): 360-4, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10925004

ABSTRACT

A new diterpenoid was isolated from the leaves of Isodon lophanthoides, together with two known diterpenoids, lophanic acid and 8(17),12,14-labdatriene-19-oic acid. The structure of the new compound was determined to be 11 beta-hydroxyisopimara-8,15-diene-3-one (1) on the basis of spectroscopic evidence.


Subject(s)
Diterpenes/chemistry , Drugs, Chinese Herbal/chemistry , Plants, Medicinal , Humans , Plant Leaves
12.
Fitoterapia ; 71(4): 417-9, 2000 Aug.
Article in English | MEDLINE | ID: mdl-10925014

ABSTRACT

A new ent-kaurane diterpenoid, phyllostachysin C (1), together with five known compounds, sculponeatins B and C, nodosin, ursolic acid and 2 alpha-hydroxyursolic acid, were isolated from the leaves of Isodon phyllostachys. The structure of 1 was elucidated on the basis of its spectral properties.


Subject(s)
Diterpenes/chemistry , Lamiaceae , Plants, Medicinal , Drugs, Chinese Herbal/chemistry , Humans , Plant Leaves
13.
J Asian Nat Prod Res ; 2(3): 161-7, 2000.
Article in English | MEDLINE | ID: mdl-11256689

ABSTRACT

A new ent-kaurane diterpenoid dimer, fritillebinide C(1) together with one known diterpenoid dimer fritillebinide B (2) were isolated from the bulbs of Fritillaria ebeiensis G.D. Yu et G.Q. Ji. Compound 1 has been determined to be ent-3beta-acetoxy-kauran-16beta,17-acetal ent-16beta-kauran-17(S)-aldehyde(1) by means of spectral analysis and chemical evidence.


Subject(s)
Diterpenes/chemistry , Liliaceae/chemistry , Chromatography , Dimerization , Diterpenes/isolation & purification , Magnetic Resonance Spectroscopy , Mass Spectrometry , Molecular Structure , Plant Structures/chemistry
14.
J Asian Nat Prod Res ; 2(3): 213-8, 2000.
Article in English | MEDLINE | ID: mdl-11256695

ABSTRACT

A novel ent-kaurane diterpenoid dimer, fritillebinide B (1) together with one known diterpenoid dimer fritillebinide A (2) were isolated from the bulbs of Fritillaria eheiensis var. purpurea G.D. Yu et P. Li. Compound 1 has been established to be ent-3beta-acetoxy-kauran-16beta,17-acetal ent-16beta-kauran-17(R)-aldehyde (1) by means of spectral analysis and chemical evidence.


Subject(s)
Diterpenes/isolation & purification , Liliaceae/chemistry , Chromatography , Diterpenes/chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Plant Structures/chemistry
15.
Lin Chuang Er Bi Yan Hou Ke Za Zhi ; 14(4): 173-4, 2000 Apr.
Article in Chinese | MEDLINE | ID: mdl-12541495

ABSTRACT

OBJECTIVE: To study HSP70 expression of middle ear mucosa in acute suppurative otitis media. METHOD: An animal model of acute middle ear infection was established by inoculating Klebsiella Pneumoniae into middle ear of guinea pigs. Animals were sacrificed 1, 3, 5 and 7 days after inoculation. The expression of Hsp70 epitope related proteins in middle ear mucosa were analysed by modified western blot test. RESULT: Very light 70 kD bands were recognized by anti Hsp70 monoclonal antibody in the unstressed group. However, middle ear mucosa of the stressed group not only showed relatively strong 70 kD bands, but also expressed more intensely 31 kD and 17 kD bands. The infected mucosa expressed one 70 kD band at 1st day, the strongest band at 3rd day, falling at 5th day; 31 kD was recognized two bands at 3rd day, the strongest bands at 5th day, falling at 7th day; two bands of 17 kD were detected in all days, with the strongest bands at 7th day. CONCLUSION: The results suggested that middle ear mucosa expressed Hsp70 and Hsp70 epitope related proteins like 31 kD and 17 kD in acute otitis media.


Subject(s)
Ear, Middle/metabolism , HSP70 Heat-Shock Proteins/biosynthesis , Klebsiella Infections/metabolism , Otitis Media, Suppurative/metabolism , Animals , Guinea Pigs , Mucous Membrane/metabolism , Otitis Media, Suppurative/microbiology
16.
J Nat Prod ; 62(7): 941-5, 1999 Jul.
Article in English | MEDLINE | ID: mdl-10425112

ABSTRACT

Six new ent-kaurane diterpenoids, lungshengenins B-G (1-6), together with three known diterpenoids, lungshengenin A (7), inflexin (8), and lushanrubescinsin C (9), were isolated from the leaves and tender branches of Isodon lungshengensis. Their structures were elucidated by means of spectroscopy, mainly 1D and 2D NMR techniques. Lungshengenins A (7), C (2), and G (6) were cytotoxic toward K562 cells, having IC(50) values equal to or less than 10 microg/mL.


Subject(s)
Antineoplastic Agents, Phytogenic/isolation & purification , Diterpenes/isolation & purification , Plants, Medicinal/chemistry , Antineoplastic Agents, Phytogenic/pharmacology , Cell Survival , Diterpenes/pharmacology , Drug Screening Assays, Antitumor , Humans , K562 Cells , Magnetic Resonance Spectroscopy
17.
J Asian Nat Prod Res ; 1(4): 251-7, 1999.
Article in English | MEDLINE | ID: mdl-11523545

ABSTRACT

Two new ent-kauranoid diterpenoid dimers, fritillebin C (1) and fritillebin D (2), were isolated from the bulbs of Fritillaria ebeiensis G.D. Yu and G.Q. Ji. Their structures were determined to be ent-16beta-hydroxy-kauran-17-yl ent-16beta3-kauran-17-oate (1); ent-16alpha-hydroxy-kauran-17-yl ent-16beta-kauran-17-oate (2) by means of spectral analysis and chemical evidence.


Subject(s)
Diterpenes/isolation & purification , Liliaceae/chemistry , Dimerization , Magnetic Resonance Spectroscopy , Models, Chemical
18.
J Asian Nat Prod Res ; 1(4): 277-84, 1999.
Article in English | MEDLINE | ID: mdl-11523548

ABSTRACT

Four new diterpenoids, melissoidesin E (1), F (2), G (4) and H (5), together with one known diterpenoid and two lignan glycosides, were isolated from aerial parts of Isodon melissoides. Their structures were established by spectral analysis and comparison with related compounds. The lignan glycosides (compounds 7 and 8) were the first examples to be isolated from the genus Isodon plants.


Subject(s)
Diterpenes/isolation & purification , Plants, Medicinal/chemistry , Magnetic Resonance Spectroscopy , Models, Chemical , Spectrometry, Mass, Fast Atom Bombardment
19.
J Asian Nat Prod Res ; 1(2): 77-86, 1998.
Article in English | MEDLINE | ID: mdl-11261217

ABSTRACT

Examination of the diterpenoid constituents of the dried leaves of Isodon angustifolius var. glabrescens led to the isolation of five new ent-kaurane diterpenoids, named as glabcensin Q-U (2-6). The structures were elucidated on the basis of spectroscopic evidences.


Subject(s)
Diterpenes/isolation & purification , Plants/chemistry , Diterpenes/chemistry , Molecular Structure , Spectrum Analysis
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