Thermodynamic model for memory.

A thermodynamic model for memory formation is proposed. Key points include: 1) Any thought or consciousness corresponds to a thermodynamic system of nerve cells. 2) The system concept of nerve cells can only be described by thermodynamics of condensed matter. 3) The memory structure is logically associated with the system structure or the normal structure of biology. 4) The development of our thoughts is processed irreversibly, and numerous states or thoughts can be generated. 5) Memory formation results from the reorganization and change of cellular structures (or memory structures), which are related to nerve cell skeleton and membrane. Their alteration can change the excitability of nerve cells and the pathway of neural impulse conduction. 6) Amnesia results from the loss of thermodynamic stability of the memory structure, which can be achieved by different ways. Some related phenomena and facts are discussed. The analysis shows that thermodynamics can account for the basic properties of memory.

Liquid metal-hydrogel composites for flexible electronics.

As an emerging functional material, liquid metal-hydrogel composites exhibit excellent biosafety, high electrical conductivity, tunable mechanical properties and good adhesion, thus providing a unique platform for a wide range of flexible electronics applications such as wearable devices, medical devices, actuators, and energy conversion devices. Through different composite methods, liquid metals can be integrated into hydrogel matrices to form multifunctional composite material systems, which further expands the application range of hydrogels. In this paper, we provide a brief overview of the two materials: hydrogels and liquid metals, and discuss the synthesis method of liquid metal-hydrogel composites, focusing on the improvement of the performance of hydrogel materials by liquid metals. In addition, we summarize the research progress of liquid metal-hydrogel composites in the field of flexible electronics, pointing out the current challenges and future prospects of this material.

Thermodynamic understanding of flower pigmentation.

We have reviewed and interpreted the thermodynamic principles for flower pigmentation. The basic thoughts are as follows: 1) any biological trait is associated with one thermodynamic system; 2) a thermodynamic system of biology cannot be physically isolated from complex thermal systems of biology but can be separately studied using thermodynamic methods; 3) a thermodynamic system of biology has all types of information, including volume, shape, and structure, unlike the traditional thermal system of gases; 4) a thermodynamic system of biology is associated with one type of biological structure that is not fully fixed but can change its conformation under different conditions; and 5) a thermodynamic system of biology shows a hierarchical structure. On the basis of these principles, several conclusions regarding flower pigmentation are obtained as follows: 1) processes of pigmentation formation can be divided into reversible and irreversible processes; 2) the reversible process is related to quantitative changes in pigments; 3) the irreversible process is related to the formation of stable pigmentation patterns that are physiologically inherited; 4) the spot pattern of color pigmentation represents an independent island of the physiological system; 5) many types of activators and inhibitors are involved in flower pigmentation production; 6) the patterns of flower pigmentation can be modulated; and 7) the evolution mechanism of organogenesis can be separated into several steps of independent thermodynamic processes. Our conclusion is that the thermodynamic system, rather than the dynamic system, is the essential and fundamental attribute of biological behaviors.

Wide Range Strain Distributions on the Electrode for Highly Sensitive Flexible Tactile Sensor with Low Hysteresis.

Flexible piezoresistive tactile sensors are widely used in wearable electronic devices because of their ability to detect mechanical stimuli. However, achieving high sensitivity and low hysteresis over a broad detection range remains a challenge with current piezoresistive tactile sensors. To address these obstacles, we designed elastomeric micropyramid arrays with different heights to redistribute the strain on the electrode. Furthermore, we mixed single-walled carbon nanotubes in the elastomeric micropyramids to compensate for the conductivity loss caused by random cracks in the gold film and increase the adhesion strength between the gold film (deposited on the pyramid surface) and the elastomer. Thus, the energy loss of the sensor during deformation and hysteresis (∼2.52%) was effectively reduced. Therefore, under the synactic effects of the percolation effect, tunnel effect, and multistage strain distribution, the as-prepared sensor exhibited a high sensitivity (1.28 × 106 kPa-1) and a broad detection range (4.51-54837.06 Pa). The sensitivity was considerably higher than those of most flexible pressure sensors with a microstructure design. As a proof of concept, the sensors were successfully applied in the fields of health monitoring and human-machine interaction.

Highly Sensitive and Omnidirectionally Stretchable Bioelectrode Arrays for In Vivo Neural Interfacing.

Flexible electrode array, a new-generation neural microelectrode, is a crucial tool for information exchange between living tissues and external electronics. Till date, advances in flexible neural microelectrodes are limited because of their high impedance and poor mechanical consistency at tissue interfaces. Herein, a highly sensitive and omnidirectionally stretchable polymeric electrode array (PEA) is introduced. Micropyramid-nanowire composite structures are constructed to increase the effective surface area of PEA, achieving an exponential reduction in impedance compared with gold (Au) and flat polypyrrole electrodes. Moreover, for the first time, a suspended umbrella structure to enable PEA with omnidirectional stretchability of up to ≈20% is designed. The PEA can withstand 1000 cycles of mechanical loads without decrease in performance. As a proof of concept, PEA is conformally attached to a rat heart and tibialis anterior muscle, and electrophysiological signals (electrocardiogram and electromyogram) of the rat are successfully recorded. This strategy provides a new perspective toward highly sensitive and omnidirectionally stretchable PEA that can facilitate the practical application of neural electrodes.

A Nonswelling Hydrogel with Regenerable High Wet Tissue Adhesion for Bioelectronics.

Reducing the swelling of tissue-adhesive hydrogels is crucial for maintaining stable tissue adhesion and inhibiting tissue inflammation. However, reported strategies for reducing swelling always result in a simultaneous decrease in the tissue adhesive strength of the hydrogel. Furthermore, once the covalent bonds break in the currently reported hydrogels, they cannot be rebuilt, and the hydrogel loses its tissue adhesive ability. In this work, a nonswelling hydrogel (named as "PAACP") possessing regenerable high tissue adhesion is synthesized by copolymerizing and crosslinking poly(vinyl butyral) with acrylic acid, gelatin, and chitosan-grafted N-acetyl-l-cysteine. The tissue adhesive strength of the obtained PAACP reaches 211.4 kPa, which is approximately ten times higher than that of the reported nonswelling hydrogels, and the hydrogel can be reused for multiple cycles. The as-prepared hydrogel shows great potential in soft bioelectronics, as muscle fatigue is successfully monitored via the electrode array and strain sensor integrated on PAACP substrates. The success of these bioelectronics offers potential applicability in the long-term diagnosis of muscle-related health conditions and prosthetic manipulations.

Molecular and thermodynamic mechanisms for protein adaptation.

Using thermal adaptation of enzymes as an example, we have proposed a molecular and thermodynamic model for protein adaptation. Key concepts: (1) The working mechanism of enzymatic reactions is not altered in protein adaptation, but the activity of the adapted enzyme is expressed under altered conditions. (2) The alteration of protein conformational stability induced by gene mutation is the fundamental cause of protein adaptation. (3) The population change in active conformations of enzymes induced by protein conformational stability in different temperature ranges is the major cause of protein adaptation. (4) The features of enzyme adaptation must be analyzed or judged by two different aspects: local population change in active conformations near a critical level of an environmental factor; and the position of the whole active conformational curve in the gradient of an environmental factor. (5) Protein adaptation represents a specific mechanism for protein regulation. Several other aspects of protein adaptation are also discussed and reviewed, and specific examples are given of enzymes showing particular types of adaptation.

Thermodynamic principles for system biology and the patterns of flower pigmentation.

The thermodynamic principles for system biology are reviewed and formulated, and then basic patterns of flower pigmentation are interpreted. Main thoughts: (1) any biological trait (color or function of a cell) is logically related to a thermodynamic system (or physiological system, signaling network of the cell), (2) the striped, speckled and circle are three basic patterns of flower pigmentation, the development of flowers is an irreversible process, (3) the patterns of flower pigmentation are formed in flower development, (4) the flower cells can change its color in a period of development and this process is controlled thermodynamically, (5) there is giant space of physiology within an organism and within its numerous thermal states can appear under different conditions. In this theory, the dominant inheritance means that a gene contributes great to the thermodynamic stability of a trait related system; different genes can be interacted or integrated thermodynamically according to their contribution to the stability of its related system. By combination of Turing theory and our views, complex patterns of pigmentation could be explained theoretically.

Strategies for interface issues and challenges of neural electrodes.

Neural electrodes, as a bridge for bidirectional communication between the body and external devices, are crucial means for detecting and controlling nerve activity. The electrodes play a vital role in monitoring the state of neural systems or influencing it to treat disease or restore functions. To achieve high-resolution, safe and long-term stable nerve recording and stimulation, a neural electrode with excellent electrochemical performance (e.g., impedance, charge storage capacity, charge injection limit), and good biocompatibility and stability is required. Here, the charge transfer process in the tissues, the electrode-tissue interfaces and the electrode materials are discussed respectively. Subsequently, the latest research methods and strategies for improving the electrochemical performance and biocompatibility of neural electrodes are reviewed. Finally, the challenges in the development of neural electrodes are proposed. It is expected that the development of neural electrodes will offer new opportunities for the evolution of neural prosthesis, bioelectronic medicine, brain science, and so on.

Electrostatic Interaction-Based High Tissue Adhesive, Stretchable Microelectrode Arrays for the Electrophysiological Interface.

The drift or fall of stretchable neural microelectrodes from the surface of wet and dynamic tissues severely hampers the adoption of microelectrodes for electrophysiological signal monitoring. Endowing the stretchable electrodes with adhesive ability is an effective way to overcome these problems. Current adhesives form tough adhesion to tissues by covalent interaction, which decreases the biocompatibility of the adhesives. Here, we fabricate a strong electrostatic adhesive (noncovalent interaction), highly conformal, stretchable microelectrode arrays (MEAs) for the electrophysiological interface. This MEA was composed of polypyrrole (PPy) as the electrode material and hydrogel as the stretchable substrate [the cross-linked and copolymerized hydrogel of 2-acrylamido-2-methylpropane sulfonic acid (AMPS), gelatin, chitosan, 2-methoxyethyl acrylate, and acrylic acid is named PAGMA]. Strong and stable electrostatic adhesion (85 kPa) and high stretchability (100%) allow for the integration of PPy MEAs based on the PAGMA hydrogel substrate (PPy-PAGMA MEAs) on diverse wet dynamic tissues. Additionally, by adjusting the concentration of AMPS in PAGMA, the hydrogel (PAGMA-1) can produce tough adhesion to many inorganic and elastomer materials. Finally, the PPy-PAGMA MEAs were toughly and conformally adhered on the rat's subcutaneous muscle and beating heart, and the rat's electrophysiological signals were successfully recorded. The development of these adhesive MEAs offers a promising strategy to establish stable and compliant electrode-tissue interfaces.

Quantitative Phosphoproteomic and Metabolomic Analyses Reveal GmMYB173 Optimizes Flavonoid Metabolism in Soybean under Salt Stress.

Salinity causes osmotic stress to crops and limits their productivity. To understand the mechanism underlying soybean salt tolerance, proteomics approach was used to identify phosphoproteins altered by NaCl treatment. Results revealed that 412 of the 4698 quantitatively analyzed phosphopeptides were significantly up-regulated on salt treatment, including a phosphopeptide covering the serine 59 in the transcription factor GmMYB173. Our data showed that GmMYB173 is one of the three MYB proteins differentially phosphorylated on salt treatment, and a substrate of the casein kinase-II. MYB recognition sites exist in the promoter of flavonoid synthase gene GmCHS5 and one was found to mediate its recognition by GmMYB173, an event facilitated by phosphorylation. Because GmCHS5 catalyzes the synthesis of chalcone, flavonoids derived from chalcone were monitored using metabolomics approach. Results revealed that 24 flavonoids of 6745 metabolites were significantly up-regulated after salt treatment. We further compared the salt tolerance and flavonoid accumulation in soybean transgenic roots expressing the 35S promoter driven cds and RNAi constructs of GmMYB173 and GmCHS5, as well as phospho-mimic (GmMYB173S59D ) and phospho-ablative (GmMYB173S59A ) mutants of GmMYB173 Overexpression of GmMYB173S59D and GmCHS5 resulted in the highest increase in salt tolerance and accumulation of cyaniding-3-arabinoside chloride, a dihydroxy B-ring flavonoid. The dihydroxy B-ring flavonoids are more effective as anti-oxidative agents when compared with monohydroxy B-ring flavonoids, such as formononetin. Hence the salt-triggered phosphorylation of GmMYB173, subsequent increase in its affinity to GmCHS5 promoter and the elevated transcription of GmCHS5 likely contribute to soybean salt tolerance by enhancing the accumulation of dihydroxy B-ring flavonoids.

On the indirect relationship between protein dynamics and enzyme activity.

The behaviors of simple thermal systems have been well studied in physical chemistry and the principles obtained from such studies have been applied to complex thermal systems, such as proteins and enzymes. But the simple application of such principles is questionable and may lead to mistakes under some circumstances. In enzymology, the transition state theory of chemical reactions has been accepted as a fundamental theory, but the role of protein dynamics in enzyme catalysis is controversial in the context of transition state theory. By studying behaviors of complex thermal systems, we have revised the Arrhenius equation and transition state theory and our model is validated in enzymology. Formally speaking, the revised Arrhenius equation is apparently similar to a conventional Arrhenius equation, but the physical meanings of its parameters differ from that of traditional forms in principle. Within this model, the role of protein dynamics in enzyme catalysis is well defined and quantified.

A thermodynamic and theoretical view for enzyme regulation.

Precise regulation is fundamental to the proper functioning of enzymes in a cell. Current opinions about this, such as allosteric regulation and dynamic contribution to enzyme regulation, are experimental models and substantially empirical. Here we proposed a theoretical and thermodynamic model of enzyme regulation. The main idea is that enzyme regulation is processed via the regulation of abundance of active conformation in the reaction buffer. The theoretical foundation, experimental evidence, and experimental criteria to test our model are discussed and reviewed. We conclude that basic principles of enzyme regulation are laws of protein thermodynamics and it can be analyzed using the concept of distribution curve of active conformations of enzymes.

Dynamic model for enzyme action.

BACKGROUND: Protein thermodynamic structure theory is an integrated approach to the study of protein dynamics and the mechanisms of enzyme catalysis. In this paper, a hypothesis arising from this theory is examined. The timescale of an enzymatic reaction (TER) gives a key to characterizing enzyme conformational changes. The aspects of timescale important in our approach are: (i) it is logically related to internal motions of the main chain of a protein; (ii) it sets the upper limit on the size or scope of protein conformational changes. Feature (i) is linked to the dynamic properties of enzyme-reactant complexes. Feature (ii) is linked to the dynamic sites of the main chain (promoting motion) involved in enzyme activity. CONCLUSION: Our analysis shows that a comprehensive understanding of enzymology can be established on the basis of protein thermodynamic structure theory.

Increased mindfulness--the active component of the mindfulness-based stress reduction program?

While there is growing consensus that Mindfulness-Based Stress Reduction (MBSR) is an effective program for patients with a wide range of health problems, little is known with regard to the processes underlying benefits seen following the program. Herein we examined the relationship between increases in mindfulness and improvements in patient outcomes. We sought to determine if there was a relationship between the practice of various types of meditation taught during the program and post-MBSR results. Eighty-three chronic ill patients provided pre- and post-MBSR data. An increase in mindfulness was significantly related to reductions in depressive symptoms, stress, medical symptoms and an increase in overall sense of coherence. However, the relationship between practice and outcomes was less evident. Future research is needed to identify which factors lead to an increase in mindfulness.

Protein flexibility as a biosignal.

Dynamic properties of a protein are crucial for all protein functions, and those of signaling proteins are closely related to the biological function of living beings. The protein flexibility signal concept can be used to analyze this relationship. Protein flexibility controls the rate of protein conformational change and influences protein function. The modification of protein flexibility results in a change of protein activity. The logical nature of protein flexibility cannot be explained by applying the principles of protein three-dimensional structure theory or conformation concept. Signaling proteins show high protein flexibility. Many properties of signaling can be traced back to the dynamic natures of signaling protein. The action mechanism of volatile anesthetics and universal cellular reactions are related to flexibility in the change of signaling proteins. We conclude that protein dynamics is an enzyme-enhanced process, called dynamicase.

In vivo measurement of protein functional changes.

Conformational changes in proteins are fundamental to all biological functions. In protein science, the concept of protein flexibility is widely used to describe protein dynamics and thermodynamic properties that control protein conformational changes. In this study, we show that urea, which has strong sedative potency, can be administered to fish at high concentrations, and that protein functional changes related to anesthesia induction can be measured in vivo. Ctenopharyngodon idellus (the grass carp) has two different types of N-methyl d-aspartate (NMDA) receptors, urea-insensitive and urea-sensitive, which are responsible for the heat endurance of fish. The urea-sensitive NMDA receptor showed high protein flexibility, the gamma aminobutyric acid (GABA) receptor showed less flexibility, and the protein that is responsible for ethanol anesthesia showed the lowest flexibility. The results suggest that an increase in protein flexibility underlies the fundamental biophysical mechanisms of volatile general anesthetics.

Protein thermodynamic structure.

Protein research is generally recognized as experimental science and knowledge of protein science is not constructed axiomatically. In this article, we show that much of our present knowledge of protein science is explainable by principles of protein thermodynamic structure theory. A deductive system for protein knowledge has been developed and several fundamental questions of protein science can be theoretically resolved.