Visible-light-driven photocatalytic degradation of ibuprofen by Cu-doped tubular C3N4: Mechanisms, degradation pathway and DFT calculation.

The copper-modified tubular carbon nitride (CTCN) with higher specific surface area and pore volume was prepared by a simple in-situ hydrolysis and self-assembly. Increased ∼4.7-fold and ∼2.3-fold degradation rate for a representative refractory water pollutant (Ibuprofen, IBP) were achieved with low-energy light source (LED, 420 ± 10 nm), as compared to graphitic carbon nitride (GCN) and tubular carbon nitride (TCN), respectively. The high efficiency of IBP removal was supported by narrow band gap (2.15 eV), high photocurrent intensity (1.10 µA/cm2) and the high surface -OH group (14.75 µg/cm3) of CTCN. According to analysis of the various reactive species in the degradation, the superoxide radical (•O2-) played a dominant role, followed by •OH and h+, responsible for IBP degradation. Furthermore, Fukui functions were employed to predict the active sites of IBP, and combined with the HPLC-MS/MS results, possible mechanisms and pathways for photocatalytic degradation were indicated. This study will lay an important scientific foundation and a possible new approach for the treatment of emerging aromatic organic pollutants in visible-light-driven heterogeneous catalytic oxidation environment.

Sex-specific impacts of thimerosal on the behaviors and brain monoaminergic systems in zebrafish (Danio rerio).

Thimerosal (THI) is the most widely used form of organic mercury in pharmaceutical and personal care products, and has become a major source of ethylmercury pollution in aquatic ecosystems. However, knowledge about its potential risk to aquatic species is limited. In this study, zebrafish were exposed to THI for 7 days, and variations in their behavioral traits, brain monoaminergic neurotransmitter contents, and related gene expression were investigated. After the 7-day exposure, THI reduced locomotor activity and thigmotaxis in males but not females. Exposure to THI increased the social interaction between females but decreased that between males. The THI exposure also significantly reduced the serotonin (5-HT), 5-hydroxyindoleacetic acid, dopamine (DA), and 3,4-dihydroxyphenylacetic acid contents in the brain of males, but only significantly decreased the DA content in females. Correlation analysis revealed that the neurochemical alterations in the brain of zebrafish play critical roles in the behavioral abnormalities induced by THI exposure. Moreover, THI also significantly altered the expression of some genes associated with the synthesis, metabolism, and receptor binding of 5-HT and DA in the brain of zebrafish. The differences in these gene expressions between female and male zebrafish exposed to THI seem to be an important mechanism underlying their sex-specific responses to this chemical. This is the first report on the sex-specific effects of THI on behaviors and brain monoaminergic neurotransmitter contents in zebrafish, which can further improve our understanding of its toxic effects on teleost.

Study on the performance of a new type of combined packing biofilm reactor treating wastewater.

The present work investigates the performance of a biofilm reactor filled with a new type of combined packing used to treat wastewater and explores a new technology approach for the application of coral sand and waste non-woven fabric. The combined packing was made of coral sand and waste non-woven fabric, which was used as a biofilm carrier to treat sewage. The experimental results showed that the removal efficiencies of COD, NH4+-N and TN in the biofilm reactor containing the combined packing were 92.9%, 72.9% and 63.2%, respectively. The maximum removal efficiencies of COD, NH4+-N and TN in the biofilm reactor containing single packing were 89.0%, 63.4% and 55.2%, respectively. The properties of the combined packing were characterized by Fourier Transform Infrared (FTIR), specific surface area, SEM and dehydrogenase activity. Infrared analysis showed that there were hydroxyl, carboxyl and carbonyl groups on the surface of coral sand and non-woven fabric which were beneficial for biofilm growth and wastewater treatment. The large pores in the interior of coral sand and non-woven fabric could provide a comfortable environment for microbes to grow and reproduce. The dehydrogenase activity of the biofilm on the surface of coral sand in the third biofilm reactor was 49.91 µgTF·g-1·h-1, which was significantly higher than that of the other two biofilm reactors. The new type of combined packing is suitable for biofilm carriers with low cost, which can be applied to actual sewage treatment projects. This study provides a reference for the practical application of the technique.

The Tobit-Unscented-Kalman-Filter-Based Attitude Estimation Algorithm Using the Star Sensor and Inertial Gyro Combination.

For the orbit operation of spacecraft, due to environmental factors, a star sensor installed on the spacecraft must have data censoring, which greatly reduces the attitude determination ability of the traditional combined-attitude-determination algorithm. To address this problem, this paper proposes an algorithm for high-precision attitude estimation based on a Tobit unscented Kalman filter. This is on the basis of establishing the nonlinear state equation of the integrated star sensor and gyroscope navigation system. The measurement update process of the unscented Kalman filter is improved. The Tobit model is used to describe the gyroscope drift when the star sensor fails. The latent measurement values are calculated using the probability statistics, and the measurement error covariance expression is derived. The proposed design is verified via computer simulations. When the star sensor fails for 15 min, the accuracy of the Tobit unscented Kalman filter based on the Tobit model is improved by approximately 90% compared to the unscented Kalman filter. Based on the results, the proposed filter can effectively estimate the error caused by the gyro drift, and the method is effective and feasible, provided there is theoretical support for the engineering practice.

Scattering analysis of a Laguerre Gaussian beam for target detection.

The propagation and scattering characteristics of a Laguerre Gaussian (LG) beam and Gaussian beam are theoretically and experimentally comparatively studied. The phase of the LG beam is almost free of scattering when the scattering is weak, resulting in a much weaker loss of transmission compared with the Gaussian beam. However, when scattering is strong, the phase of the LG beam is completely disturbed by it, and the transmission loss of the LG beam is stronger than that of the Gaussian beam. Moreover, the phase of the LG beam becomes stabler as the topological charge increases, and the beam radius increases as well. Therefore, the LG beam is suitable for short-range target detection in a weak scattering medium, not for long-range in a strong scattering medium. This work will contribute to the development of target detection, optical communication, and other applications of orbital angular momentum beams.

An Electromagnetic-Driven Microshutter Array in a Field-of-View Gated Image System for All-Time Star Sensors.

Aiming at the application requirements of a field of view (FOV) gated imaging system for all-time star sensors, a key device of a microshutter array with large unit size, high duty cycle, and fast response speed based on the electromagnetic actuation is designed. The proposed microshutter array adopts the principle that the current-carrying coil is subjected to the magnetic force in the magnetic field. The coil element is deflected by the loading current and acts as a light barrier in realizing the optical switch function. The effects of the coil element parameters on the magnetic force torque, torsion beam resistance torque, and switch response time are analyzed, and the structural parameters of the coil element are determined. A sample of the proposed microshutter array based on the electromagnetic actuation with a 4-mm period and a 2.8-mm aperture is fabricated and tested. The test results demonstrate the good switching function of the proposed microshutter array and show that the switch response time of the microshutter element is approximately 2.5 ms. This proposed microshutter array is used to gate an instantaneous small FOV to suppress the sky's background radiation and make a FOV-gated imaging system realize the multi-stars detection by switching the gated FOV rapidly. This will solve the problem that only one star can be detected within the FOV by a traditional all-time star tracker and promote the all-time star sensor to realize star pattern recognition and autonomous astronomical navigation in the daytime.

Optical imaging system for an all-time star sensor based on field of view gated technology.

The wide field of view (FOV) of traditional star sensor optical systems restricts the ability to suppress atmospheric background. An optical imaging system for an all-time star sensor based on FOV gated technology is proposed. In this system, a wide FOV telescope is used to observe a large sky area containing multiple stars. A microlens and microshutter array is employed to subdivide the wide FOV and gate a narrow FOV to suppress atmospheric background radiation. Assisted by a common imaging lens, each set of microlens and microshutter elements corresponds to a FOV gated imaging channel. With the rapid switching of gated FOV, multiple stellar images are obtained on a common detection during daytime. As an example, a FOV gated optical imaging system with 0.4° gated FOV and 61 imaging channels is designed. In addition, a simplified prototype is developed, and a preliminary experiment of FOV gated imaging is performed near the ground. The results verify the capability of multiple stellar detections during daytime. The proposed optical imaging system has a strong capability of suppressing atmospheric background radiation and provides sufficient FOV gated imaging channels to enhance the probability of detecting multiple stars. It provides an effective technical way to develop all-time star sensors based on star pattern recognition and enables a completely autonomous attitude determination possible for platforms inside the atmosphere during daytime.

ISSM-ELM - a guide star selection for a small-FOV star sensor based on the improved SSM and extreme learning machine.

The construction of a guide star catalog is crucial for a star sensor to achieve accurate star map recognition and attitude determination. At present, the methods of a guide star catalog for a large field of view (FOV) star sensor have been relatively mature. However, for a small-FOV star sensor, there are still certain problems, such as a large storage capacity of a guide star catalog, uneven distribution of stars, and easy occurrence of voids. To address these problems, we propose a construction method of a small-FOV star sensor guide star catalog based on the combination of the improved spherical spiral method (ISSM) and extreme learning machine (ELM) named the ISSM-ELM. First, a spiral reference point is used as an optical axis pointing of the star sensor, and the guide stars are preliminarily screened based on the star-diagonal distance between the star and the reference point, and the star-density and magnitude characteristics of the guide star. Then the ELM is used to supplement the guide star empty sky area to construct an integrity guide star catalog. The experimental results demonstrate that the proposed method can reduce the storage capacity of the guide star catalog, and improve its uniformity, integrity, and average brightness.

High Update Rate Attitude Measurement Method of Star Sensors Based on Star Point Correction of Rolling Shutter Exposure.

Attitude update rate is one of the important indicators of star sensor performance. In order to resolve the problem of the low attitude update rate of star sensors, this paper proposes a star sensor attitude update method based on star point correction of rolling shutter exposure. Based on the characteristics of the asynchronous exposure of the rolling shutter, recursive estimation of the motion attitude and the corrected star point information were combined to realize multiple updates of the attitude in a single frame of the star map. Simulation and experimental results proved that the proposed method could increase the attitude update rate of a star sensor by 15 times, up to 150 Hz.

Star Sensor Denoising Algorithm Based on Edge Protection.

Single-pixel noise commonly appearing in a star sensor can cause an unexpected error in centroid extraction. To overcome this problem, this paper proposes a star image denoising algorithm, named Improved Gaussian Side Window Filtering (IGSWF). Firstly, the IGSWF algorithm uses four special triangular Gaussian subtemplates for edge protection. Secondly, it exploits a reconstruction function based on the characteristic of stars and noise. The proposed IGSWF algorithm was successfully verified through simulations and evaluated in a star sensor. The experimental results indicated that the IGSWF algorithm performed better in preserving the shape of stars and eliminating the single-pixel noise and the centroid estimation error (CEE) value after using the IGSWF algorithm was eight times smaller than the original value, six times smaller than that after traditional window filtering, and three times smaller than that after the side window filtering.

Multi-Field Interference Simultaneously Imaging on Single Image for Dynamic Surface Measurement.

To obtain the dynamic surface of high-frequency vibrating mirrors (VMs), a novel method involving multi-field interference (MFI) pattern imaging on a single image is proposed in this paper. Using multiple reflections and refractions, the proposed method generates three interference patterns at the same time, which improves the traditional time-series methods where a single interference pattern can be obtained at one time. Experimental results show that a series of MFI patterns can be obtained on a single image, with the laser repetition frequency (LRF) ranging from 200 Hz to 10 Hz, and the frame rate of the camera at 10 Hz. Particularly if the LRF (10 Hz) is equal to the frame rate of image, crosstalk is avoided completely, which is particularly desirable in dynamic surface measurement. In summary, the MFI imaging method provides an effective way for VM dynamic surface measurement.

Sensitive and selective determination of butyl benzyl phthalate from environmental samples using an enzyme immunoassay.

Increased awareness of phthalic acid esters (PAEs) toxicity has given rise to a dramatic increase in concern about the determination of these contaminations in the environment. In this paper, a sensitive, selective and rapid enzyme immunoassay of ELISA based on polyclonal antibody for detecting butyl benzyl phthalate (BBP) was developed and applied in the environmental water and soil samples. The hapten of BBP was synthesized, then applied to prepare artificial antigen and produce polyclonal antibody capable of specific recognizing BBP. From the optimal standard curve of ELISA for BBP, the values of LOD (limit of detection, IC10) and IC50 were 2.5 and 79.4 ng/mL, respectively. The ELISA showed high specificity, with the cross-reactivity toward BBP analogs < 9.6%. The satisfactory accuracy and precision were demonstrated by the recoveries of 76-116% and coefficient of variations (CVs) of 4.7-13.7%. Furthermore, BBP contamination was investigated at 3.1-25.2 ng/mL in real water samples and 4.2-76.4 ng/g in real soil samples (with the detection rate of 55% in 20 samples) by the developed ELISA, which also had shown a good correlation with that the results obtained by HPLC. All of this indicated that the developed enzyme immunoassay could be applied for sensitive and selective determination of BBP contamination in the environmental samples. Furthermore, the strategy of BBP hapten synthesis and an alternative method of BBP determination could be provided.

A Convenient Calibration Method for LRF-Camera Combination Systems Based on a Checkerboard.

In this paper, a simple and easy high-precision calibration method is proposed for the LRF-camera combined measurement system which is widely used at present. This method can be applied not only to mainstream 2D and 3D LRF-cameras, but also to calibrate newly developed 1D LRF-camera combined systems. It only needs a calibration board to record at least three sets of data. First, the camera parameters and distortion coefficients are decoupled by the distortion center. Then, the spatial coordinates of laser spots are solved using line and plane constraints, and the estimation of LRF-camera extrinsic parameters is realized. In addition, we establish a cost function for optimizing the system. Finally, the calibration accuracy and characteristics of the method are analyzed through simulation experiments, and the validity of the method is verified through the calibration of a real system.

Scale Estimation and Correction of the Monocular Simultaneous Localization and Mapping (SLAM) Based on Fusion of 1D Laser Range Finder and Vision Data.

This article presents a new sensor fusion method for visual simultaneous localization and mapping (SLAM) through integration of a monocular camera and a 1D-laser range finder. Such as a fusion method provides the scale estimation and drift correction and it is not limited by volume, e.g., the stereo camera is constrained by the baseline and overcomes the limited depth range problem associated with SLAM for RGBD cameras. We first present the analytical feasibility for estimating the absolute scale through the fusion of 1D distance information and image information. Next, the analytical derivation of the laser-vision fusion is described in detail based on the local dense reconstruction of the image sequences. We also correct the scale drift of the monocular SLAM using the laser distance information which is independent of the drift error. Finally, application of this approach to both indoor and outdoor scenes is verified by the Technical University of Munich dataset of RGBD and self-collected data. We compare the effects of the scale estimation and drift correction of the proposed method with the SLAM for a monocular camera and a RGBD camera.

Property of filler-loaded magnetic ferrite from plastic waste bottle used to treat municipal domestic sewage.

The present work investigates the properties of self-made magnetic filler from plastic waste bottle and explores a new technology approach of waste plastic resource utilization. The magnetic filler was prepared by air plasma modification and loading magnetic ferrite on the plastic strip from waste plastic bottle. The surface properties of magnetic filler were characterized by Atomic Force Microscope (AFM), contact angle system and Fourier Transform Infrared (FTIR). AFM images of original and modified plastic strip showed that low-temperature plasma treatment markedly increased the surface roughness of plastic strip. The mean roughness (Ra) of plastic strip rose from 1.116 to 5.024 nm. FTIR spectra indicated that a lot of polar oxygenic groups were introduced onto the surface of plastic by plasma modification. Modification by low-temperature plasma increased the hydrophilicity of plastic strip surface. When treatment time is 40 s, water contact angle of plastic strip surface reduced from 78.2° of original plastic strip to 25.3°. When used in bioreactor, magnetic filler had very favorable microenvironment for microorganism growth. Magnetic filler was more efficient for removing chemical oxygen demand (COD) and [Formula: see text] in sewage than nonmagnetic filler. The resource utilization of plastic wastes will become reality if the magnetic filler is applied widely.

Immunomagnetic bead-based biotin-streptavidin system for highly efficient detection of aflatoxin B1 in agricultural products.

The potential homogeneous assay employing immunomagnetic beads (IMB) has been receiving attention as a screening tool in food-safety control; the method is simple, efficient, and does not require long incubation times or complex separation steps. In this study, a homogeneous immunoassay has been successfully developed and applied in the determination of aflatoxin B1 (AFB1) contamination in agricultural products by coupling IMB and the biotin-streptavidin (BSA) (BSA-IMB) system. Under optimal conditions, the limit of detection (LOD, IC10), half-maximal inhibition concentration (IC50) and detection range (IC20-IC80) of BSA-IMB are 0.00579, 0.573 and 0.0183-17.9 ng mL-1, respectively, for AFB1. The detection of AFB1 by BSA-IMB can be achieved in 40 min (ELISA needs at least 180 min). The cross-reactivities of BSA-IMB with its analogues are negligible (<3.82%); these results indicate high selectivity. The spiked recoveries are in the range from 89.6 to 118.2% with relative standard deviations (RSDs) of 3.4 to 13.2% for AFB1 in agricultural product samples. Furthermore, the results of BSA-IMB for authentic samples show reliability and high correlation of 0.9928 with an HPLC-fluorescence detector. The proposed BSA-IMB system is demonstrated to be a satisfactory tool for homogeneous, efficient, sensitive, and alternative detection of AFB1 in a wide detection range for agricultural product samples.

Histone deacetylase inhibitor valproic acid (VPA) promotes the epithelial mesenchymal transition of colorectal cancer cells via up regulation of Snail.

Histone deacetylase inhibitors (HDACIs) have been shown to have antiproliferative activity through cell-cycle arrest, differentiation, and apoptosis in colorectal cancer (CRC) cells. Our present study revealed that one HDAC inhibitor, valproic acid (VPA), can obviously promote in vitro motility of HCT-116 and SW480 cells. VPA treatment significantly down regulates the expression of epithelial markers E-Cadherin (E-Cad) and Zona occludin-1(ZO-1) while up regulates the mesenchymal markers Vimentin (Vim) and N-cadherin (N-Cad), suggesting that VPA can trigger the epithelial-mesenchymal transition (EMT) of CRC cells. VPA treatment significantly increases the expression and nuclear localization of Snail, the key transcription factors of EMT. Snail knockdown by siRNAs obviously reverses VPA induced EMT of HCT-116 and SW480 cells. Further, VPA can decrease the ubiquitination, increase the acetylation, and then elevate the stabilization of Snail. VPA also increases the phosphorylation of Akt/GSK-3ß. The inhibitor of PI3K/Akt, LY2994002, significantly attenuates VPA induced phosphorylation of Akt and GSK-3ß and up regulation of Snail and Vim. Collectively, our data reveal that VPA can trigger the EMT of CRC cells via up regulation of Snail through AKT/GSK-3ß signals and post-transcriptional modification. It suggests that more attention should be paid when VPA used as a new anticancer drug for CRC patients.

Effect of p38 mitogen-activate protein kinase on MUC5AC protein expression of bile duct epithelial cells in hepatolithiasis patients.

Primary hepatolithiasis is a common bile duct disease with benign nature but complicated mechanisms. Current studies have revealed its correlation with cytokine release by chronic inflammation, which also increased mucin (MUC) synthesis. This study investigated the role of p38 mitogen-activated protein kinase (MAPK) in regulating cytokine release and mucin synthesis, in an attempt to elucidate the role of p38 signaling molecule in the pathogenesis of hepatolithiasis. In human intrahepatic bile duct endothelial cells (HIBECs), lipoprotein (LPS) was used to induce the high expression of MUC. Small interference RNA (siRNA) was then used to silencing p38 gene expression. Cytokines including interleukin (IL)-1ß and tumor necrosis factor (TNF)-α were measured, along with MUC5AC protein and mRNA expression assay. The interference of p38 gene expression inhibited the release of IL-1ß and TNF-α in cultured cells. It also depressed both mRNA and protein levels of MUC5A. P38 MAPK signal pathway may be involved in the formation and progression of hepatolithiasis. This study provides potential new strategy for treating hepatolithiasis using p38 MAPK signal pathway as the drug target.