ABSTRACT
In recent years, connected and automated vehicles (CAV) have been a transformative technology that is expected to reduce emissions and change and improve the safety and efficiency of the mobilities [...].
Subject(s)
Automobile Driving , Accidents, Traffic , Autonomous Vehicles , Motor VehiclesABSTRACT
The resistance of cancer to chemotherapeutic agents is a major obstacle during chemotherapy. Clinical multidrug resistance (MDR) is commonly mediated by membrane drug efflux pumps, including ATPbinding cassette subfamily B member 1, also termed P-glycoprotein (P-gp). P-gp is a membrane transporter encoded by the MDR1 gene. The current study aimed to investigate the impact of psoralen on the expression and function of Pgp. The 10% inhibitory concentration (IC10) of psoralen, and its capacity to reduce MDR in adriamycin (ADR)resistant MCF7/ADR cells were determined using MTT assay. The ability of psoralen to modulate the transport activity of Pgp in MCF7/ADR cells was evaluated by measuring the accumulation and efflux of rhodamine 123 (Rh 123) and adriamycin with flow cytometry. The present study evaluated the mRNA level of MDR1 in MCF7 and MCF7/ADR cells treated with psoralen using reverse transcription-quantitative polymerase chain reaction. The protein expression level of Pgp was examined by western blot analysis. The current study demonstrated that the IC10 of psoralen in MCF7/ADR cells was 8 µg/ml. At 8 µg/ml, psoralen reduced MDR and the sensitivity of the MCF7/ADR cells to ADR compared with untreated cells. Additionally, psoralen significantly increased the intracellular accumulation of ADR and Rh 123. However, the IC10 of psoralen did not affect the protein expression levels of Pgp or mRNA levels of MDR1 (P>0.05). Psoralen reduces MDR by inhibiting the efflux function of Pgp, which may be important for increasing the efficiency of chemotherapy and improving the clinical protocols aiming to reverse P-gp-mediated MDR.