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1.
Ecotoxicol Environ Saf ; 223: 112605, 2021 Oct 15.
Article in English | MEDLINE | ID: mdl-34371453

ABSTRACT

The global phase-out has decreased the use of polybrominated diphenyl ethers (PBDEs), thereby, rapidly increasing the production and use of their important surrogates, organophosphorus flame retardants (OPFRs). Currently, OPFRs are often found at higher levels in the environments compared to PBDEs. Although the two typical OPFRs, tris (1,3-dichloroisopropyl) phosphate (TDCIPP) and triphenyl phosphate (TPhP), have been frequently detected in marine environments with significant concentrations, their toxicity to marine organisms remains unknown. We used Oryzias melastigma to investigate and compare their developmental toxicity in marine organisms through two-generational chronic exposure. The results showed that TDCIPP and TPhP exposure shortened the body length and length of the pectoral fin of O. melastigma. Both TDCIPP and TPhP deformed the pectoral fins in the 1st fry and caused spinal curvature in adult fish. Therefore, these two chemicals may pose potential risks to marine fish and marine ecosystems. Further studies suggested that although these two chemicals caused similar developmental bone toxicity, they had different modes of modulating the expression of bone developmental genes such as, bmp4, bmp2 and runx2.


Subject(s)
Flame Retardants , Oryzias , Animals , Ecosystem , Flame Retardants/toxicity , Organophosphates , Organophosphorus Compounds
2.
Sci Rep ; 11(1): 1544, 2021 01 15.
Article in English | MEDLINE | ID: mdl-33452372

ABSTRACT

Soil microbe is crucial to a healthy soil, therefore its diversities and abundances under different conditions are still need fully understand.The aims of the study were to characterize the community structure and diversity of microbe in the rhizosphere soil after continuous maize seed production, and the relationship between the disease incidence of four diseases and the variation of the rhizosphere microbe. The results showed that different fungal and bacterial species were predominant in different cropping year, and long-term maize seed production had a huge impact on structure and diversity of soil microbial. Ascomycota and Mortierellomycota were the dominant fungal phyla and Mortierella and Ascomycetes represented for a large proportion of genus. A relative increase of Fusarium and Gibberella and a relative decrease of Mortierella, Chrysosporium, Podospora, and Chaetomium were observed with the increase of cropping year. Pathogenic Fusarium, Curvularia, Curvularia-lunata, Cladosporium, Gibberella-baccata, and Plectosphaerellaceae were over-presented and varied at different continuous cropping year, led to different maize disease incidence. Proteobacteria and Actinobacteria ranked in the top two of all bacterial phyla, and genus Pseudarthrobacter, Roseiflexus and RB41 dominated top 3. Haliangium and Streptomyces decreased with the continuous cropping year and mono-cropping of maize seed production increased disease incidence with the increase of cropping year, while the major disease was different. Continuous cropping of maize seed production induced the decrease of protective microbe and biocontrol genera, while pathogenic pathogen increased, and maize are in danger of pathogen invasion. Field management show great effects on soil microbial community.


Subject(s)
Rhizosphere , Seeds/microbiology , Zea mays/microbiology , Agriculture/methods , Bacteria/classification , Biodiversity , Microbiota/physiology , Plant Diseases/microbiology , Plant Roots/microbiology , Seeds/growth & development , Soil/chemistry , Soil Microbiology , Zea mays/growth & development
3.
PLoS One ; 14(3): e0211613, 2019.
Article in English | MEDLINE | ID: mdl-30840625

ABSTRACT

A fungus identified as Aspergillus aculeatus was used to biotransform genistein and glycosides to polyhydroxylated isoflavones. The strain was identified on the basis of colony morphology features and ITS rDNA sequence analysis. Phylogenetic tree was constructed to determine its taxonomic status. Genistein and glycosides were transformed by Aspergillus aculeatus to 5,7,8,4'- tetrahydroxyisoflavone. The chemical structure of the product was identified by high performance liquid chromatography(HPLC), liquid chromatography-mass spectrometry(LC/MS), Infrared spectroscopy (IR) and NMR spectrometer methods. The ITS rDNA sequence of the strain had 100% similarity with Aspergillus. Furthermore, it was ultimately identified as Aspergillus aculeatus. The metabolite of genistein and glycosides was identified as 5,7,8,4'-tetrahydroxyisoflavone. 120 mg 5,7,8,4'-tetrahydroxyisoflavone was made from 20 g fructus sophorae, which was bioconverted unconditionally by Aspergillus aculeatus for 96 h, and the purity was 96%. On the basis of the findings, Aspergillus aculeatus was a novel strain with specific ability to convert genistein and glycosides into 5,7,8,4'-tetrahydroxyisoflavone which had potential applications.


Subject(s)
Aspergillus/metabolism , Genistein/metabolism , Isoflavones/metabolism , Biotransformation/physiology , Chromatography, High Pressure Liquid/methods , DNA, Ribosomal/metabolism , Glycosides/metabolism , Magnetic Resonance Spectroscopy/methods , Phylogeny , Spectrophotometry, Infrared/methods , Tandem Mass Spectrometry/methods
4.
Aquat Toxicol ; 208: 168-178, 2019 Mar.
Article in English | MEDLINE | ID: mdl-30677712

ABSTRACT

As a newly emerging environmental contaminant, tributyl phosphate (TBP) is an additive flame retardant of high production volume that is frequently detected in biota and the environment. Despite evidence that TBP is a potential threat to marine organisms, ecotoxicology data for TBP in marine organisms at low trophic levels are scarce. In this study, the acute toxicological effect of TBP on the marine phytoplankton Phaeodactylum tricornutum was thoroughly investigated, and the possible mechanism was explored. The results showed that TBP at concentrations ≥0.2 mg L-1 significantly inhibited P. tricornutum growth in a clear dose-response manner, with 72-h EC10, EC20, EC50 and EC90 values of 0.067, 0.101, 0.219 and 0.716 mg L-1, respectively. Algal cells treated with TBP exhibited distorted shapes, ruptured cell membranes and damaged organelles, especially mitochondria. Additionally, apoptosis was triggered, followed by a decrease in mitochondrial membrane potential, indicating that cellular damage occurred during exposure. Although the activities of two antioxidant enzymes, superoxide peroxidase and catalase, were upregulated by TBP at 1.2 mg L-1, excess reactive oxygen species (ROS) and malondialdehyde still accumulated in algal cells after exposure, suggesting that the cells experienced oxidative stress. Moreover, both growth inhibition and apoptosis were positively correlated with ROS levels and were ameliorated by pretreatment with the ROS scavenger N-acetyl-l-cysteine. Taken together, the results indicate that TBP exposure leads to growth inhibition and cellular damage in P. tricornutum, and a ROS-mediated pathway might contribute to these observed toxicological effects.


Subject(s)
Diatoms/metabolism , Organophosphates/toxicity , Reactive Oxygen Species/metabolism , Acetylcysteine/pharmacology , Apoptosis/drug effects , Catalase/metabolism , Diatoms/drug effects , Diatoms/growth & development , Diatoms/ultrastructure , Malondialdehyde/metabolism , Membrane Potential, Mitochondrial/drug effects , Mitochondria/drug effects , Mitochondria/metabolism , Oxidative Stress/drug effects , Phytoplankton/drug effects , Superoxide Dismutase/metabolism , Water Pollutants, Chemical/toxicity
5.
Environ Sci Pollut Res Int ; 23(7): 6217-26, 2016 Apr.
Article in English | MEDLINE | ID: mdl-26606935

ABSTRACT

Intensive corn seed production in Northwest of China produced large amounts of furfural residues, which represents higher treatment cost and environmental issue. The broad calcareous soils in the Northwest of China exhibit low organic matter content and high pH, which led to lower fertility and lower productivity. Recycling furfural residues as soil organic and nutrient amendment might be a promising agricultural practice to calcareous soils. A 3-year field study was conducted to evaluate the effects of furfural as a soil amendment on corn seed production on calcareous soil with compared to biogas residues. Soil physical-chemical properties, soil enzyme activities, and soil heavy metal concentrations were assessed in the last year after the last application. Corn yield was determined in each year. Furfural residue amendments significantly decreased soil pH and soil bulk density. Furfural residues combined with commercial fertilizers resulted in the greater cumulative on soil organic matter, total phosphorus, available phosphorus, available potassium, and cation exchange capacity than that of biogas residue. Simultaneously, urease, invertase, catalase, and alkaline phosphatase increased even at the higher furfural application rates. Maize seed yield increased even with lower furfural residue application rates. Furfural residues resulted in lower Zn concentration and higher Cd concentration than that of biogas residues. Amendment of furfural residues led to higher soil electrical conductivity (EC) than that of biogas residues. The addition of furfural residues to maize seed production may be considered to be a good strategy for recycling the waste, converting it into a potential resource as organic amendment in arid and semi-arid calcareous soils, and may help to reduce the use of mineral chemical fertilizers in these soils. However, the impact of its application on soil health needs to be established in long-term basis.


Subject(s)
Biofuels/analysis , Furaldehyde/analysis , Zea mays/chemistry , Agriculture/methods , China , Fertilizers/analysis , Metals, Heavy/analysis , Phosphorus/analysis , Seeds/chemistry , Seeds/growth & development , Soil/chemistry , Zea mays/growth & development
6.
J Photochem Photobiol B ; 153: 261-6, 2015 Dec.
Article in English | MEDLINE | ID: mdl-26479585

ABSTRACT

The silver nanoparticles (AgNPs) were green synthesized using Cirsium arvense plant extract as a reducing and stabilizing agent, with superior photo inactivation activity against Escherichia coli (E. coli). The synthesized AgNPs had crystalline structure and were characterized by UV-vis spectroscopy, XRD, HRTEM, SEM, EDX and FT-IR. The formation of nanoparticles was observed at different pH and different plant extract concentrations and it was found that at higher pH (pH>6) and at lower concentration (10 mL), the reducing and stabilizing efficiency of plant extract was increased. The synthesized AgNPs had small size (<15 nm) and spherical shape. The AgNPs were evaluated for antibacterial activity against E. coli. Before transferring it to antibacterial activity, it was placed under visible light for 120 min. The same experiment was performed in dark as a control medium. The photo irradiated AgNPs were observed to be more effective against E. coli. The results showed, that the diameter of zone of inhibition of visible light irradiated AgNPs against E. coli was 23 (±0.5)mm and in dark was 11 (±0.4)mm.


Subject(s)
Escherichia coli/drug effects , Light , Metal Nanoparticles , Silver/pharmacology , Cirsium/chemistry , Cirsium/metabolism , Escherichia coli/radiation effects , Hydrogen-Ion Concentration , Metal Nanoparticles/chemistry , Metal Nanoparticles/ultrastructure , Microbial Sensitivity Tests , Microscopy, Electron, Transmission , Particle Size , Plant Extracts/chemistry , Silver/chemistry , Spectroscopy, Fourier Transform Infrared
7.
Environ Sci Pollut Res Int ; 21(12): 7586-95, 2014 Jun.
Article in English | MEDLINE | ID: mdl-24604271

ABSTRACT

To evaluate the effects of manure application on continuous maize seed production, 10-year cattle manure on soil properties, heavy metal in soil and plant were evaluated and investigated in calcareous soil. Results showed that manure application increased soil organic matter, total and available nutrients, pH, and electrical conductivity (EC), and the most massive rate caused the highest increase. Manure application led to an increase in exchangeable fraction and an increase of availability of heavy metal. Residual fraction was dominant among all metals, followed by the fraction bound to Fe and Mn oxides. Manure application involved accumulation of heavy metal on corn, but the accumulation in the stem is higher than that in the seed. Manure application led to a high deficiency of total Zn and high accumulation of total Cd in the soil of corn seed production, which should be a risk for safety seed production in calcareous soil in Northwest China.


Subject(s)
Manure , Metals, Heavy/analysis , Plant Stems/chemistry , Seeds/chemistry , Soil Pollutants/analysis , Zea mays , Agriculture , Animals , Cattle , China , Electric Conductivity , Fertilizers , Hydrogen-Ion Concentration
8.
Sheng Wu Gong Cheng Xue Bao ; 25(8): 1144-50, 2009 Aug.
Article in Chinese | MEDLINE | ID: mdl-19938450

ABSTRACT

To study the effect of exogenous oxygen, we added water solution of paraquat to 7 d cultures of Coriolus versicolor for the next 148 h. Enzyme exudation and biochemical process were investigated on the addition of paraquat. We found that compared with the control (without paraquat), the addition of 30 micromol/L paraquat stimulated the activity of manganese dependent peroxidase (MnP), lignin peroxidase (LiP), and laccases (Lac) 7, 2.5 and 1.3 times, respectively. Also, addition of paraquat enhanced activity of superoxide dismutase (SOD) and catalase (CAT) in the first 48 h. Impact of paraquat on ligninolytic enzymes was significant than that on antioxidant enzyme. Addition of paraquat enhanced phenolic compounds and formaldehyde of cultures too. And concentration of malondialdehyde was increased in the first 24 h. The results showed that addition of paraquat promoted oxidative stress, but the antioxidant systems of the fungal strain are sufficient to prevent mycelia from oxidative stress. As exogenous oxygen, paraquat might be a useful substrate in degradation of lignocellulose.


Subject(s)
Fungi/enzymology , Lignin/metabolism , Paraquat/pharmacology , Peroxidases/metabolism , Fungi/drug effects , Oxidative Stress
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