ABSTRACT
BACKGROUND: Osteonecrosis of the femoral head (ONFH) is a common hip joint disease, which is more harmful and seriously affects the lives of patients. This study aims to clarify the regulatory mechanism of lncRNA FGD5-AS1 in ONFH. METHODS: The expression of the protein and mRNA was detected by RT-qPCR and Western blot assay. The regulatory mechanism of lncRNA FGD5-AS1 was detected by the dual-luciferase reporter assay, CCK-8 assay, and flow cytometry assay. RESULTS: Dex can inhibit cell proliferation and differentiation and induce apoptosis in hBMSCs in a dose-dependent manner. Overexpression of lncRNA FGD5-AS1 promoted cell proliferation and restrained apoptosis in Dex-treated hBMSCs. In addition, lncRNA FGD5-AS1 acts as a sponge for miR-296-5p. Also, miR-296-5p directly targets STAT3. More importantly, miR-296-5p and STAT3 can affect the function of lncRNA FGD5-AS1 in Dex-treated hBMSCs. CONCLUSION: lncRNA FGD5-AS1 promotes cell proliferation and inhibits apoptosis in steroid-induced ONFH through acting as a sponge for miR-296-5p and upregulation of STAT3.
Subject(s)
MicroRNAs , Osteonecrosis , RNA, Long Noncoding , Apoptosis/genetics , Bone Marrow/metabolism , Cell Proliferation , Femur Head/metabolism , Guanine Nucleotide Exchange Factors/genetics , Guanine Nucleotide Exchange Factors/metabolism , Humans , MicroRNAs/genetics , MicroRNAs/metabolism , Osteonecrosis/chemically induced , Osteonecrosis/genetics , RNA, Long Noncoding/genetics , STAT3 Transcription Factor/genetics , STAT3 Transcription Factor/metabolism , SteroidsABSTRACT
OBJECTIVE: To establish a computer-aided three-dimensional visualization operation simulation system based on Mimics and Unigraphics NX software to provide reliable evidence for accurate preoperative surgical planning. METHODS: The preoperative CT scans of 5 patients with intertrochanteric fractures were used for three-dimensional reconstruction of intertrochanteric fractures using Mimics software. Three-dimensional reconstruction of the surgical instruments was carried out using the modeling function of Unigraphics NX software, whose assembly function was used to visualize the simulates internal fixations of intertrochanteric fractures with dynamic hip screw (DHS) system. The operative procedures simulated by Unigraphics NX were analyzed preoperatively. RESULTS: The virtual surgery procedures were clearly and vividly visualized in three dimensions. The fracture reduction and surgical effects could be predicted using this system. CONCLUSIONS: This system of three-dimensional visualization of virtual surgery for intertrochanteric fractures has important values in surgical planning, risk assessment and clinical training, and can help improve the reliability and outcome of orthopedic surgery.
