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1.
Sci Rep ; 14(1): 16147, 2024 Jul 12.
Article in English | MEDLINE | ID: mdl-38997340

ABSTRACT

Network traffic anomaly detection, as an effective analysis method for network security, can identify differentiated traffic information and provide secure operation in complex and changing network environments. To avoid information loss caused when handling traffic data while improving the detection performance of traffic feature information, this paper proposes a multi-information fusion model based on a convolutional neural network and AutoEncoder. The model uses a convolutional neural network to extract features directly from the raw traffic data, and a AutoEncoder to encode the statistical features extracted from the raw traffic data, which are used to supplement the information loss due to cropping. These two features are combined to form a new integrated feature for network traffic, which has the load information from the original traffic data and the global information of the original traffic data obtained from the statistical features, thus providing a complete representation of the information contained in the network traffic and improving the detection performance of the model. The experiments show that the classification accuracy of network traffic anomaly detection using this model outperforms that of classical machine learning methods.

2.
J Exp Bot ; 74(14): 4077-4092, 2023 08 03.
Article in English | MEDLINE | ID: mdl-37085949

ABSTRACT

Plant growth and development rely heavily on cyclins, which comprise an important class of cell division regulators. D-type cyclins (CYCDs) are responsible for the rate-limiting step of G1 cells. In the plant kingdom, despite the importance of CYCDs in herbaceous plants, there is little knowledge of these proteins in perennial woody plants. Here, the gene of a nucleus-localized cyclin, PsnCYCD1;1, was cloned from Populus simonii × P. nigra. PsnCYCD1;1 was highly expressed in tissues with active cell division, especially the leaf buds, and could be induced by sucrose and phytohormones. Moreover, overexpression of PsnCYCD1;1 in poplar could stimulate cell division, resulting in the generation of small cells and causing severe morphological changes in the vascular bundles, resulting in 'S'-shaped tortuous stems and curled leaves. Furthermore, transcriptomic analysis revealed that endogenous genes related to cell division and vascular cambium development were significantly up-regulated in the transgenic plants. In addition, using yeast two-hybrid and bimolecular fluorescence complementation assays PsnCDKA1, PsnICK3, and PsnICK5 were identified as proteins interacting with PsnCYCD1;1. Our study demonstrates that PsnCYCD1;1 accelerates plant cell division and participates in secondary growth of vascular bundles in poplar.


Subject(s)
Populus , Plant Vascular Bundle/metabolism , Cell Division , Plant Growth Regulators/metabolism , Plants, Genetically Modified/genetics , Plants, Genetically Modified/metabolism , Cyclins/genetics , Gene Expression Regulation, Plant , Plant Proteins/genetics , Plant Proteins/metabolism
3.
Front Plant Sci ; 13: 868731, 2022.
Article in English | MEDLINE | ID: mdl-35463407

ABSTRACT

The D-type cyclin (CYCD) gene, as the rate-limiting enzyme in the G1 phase of cell cycle, plays a vital role in the process of plant growth and development. Early studies on plant cyclin mostly focused on herbs, such as Arabidopsis thaliana. The sustainable growth ability of woody plants is a unique characteristic in the study of plant cyclin. Here, the promoter of PsnCYCD1;1 was cloned from poplar by PCR and genetically transformed into tobacco. A strong GUS activity was observed in the areas with vigorous cell division, such as stem tips, lateral buds, and young leaves. The PsnCYCD1;1-GFP fusion expression vector was transformed into tobacco, and the green fluorescence signal was observed in the nucleus. Compared with the control plant, the transgenic tobacco showed significant changes in the flower organs, such as enlargement of sepals, petals, and fruits. Furthermore, the stems of transgenic plants were slightly curved at each stem node, the leaves were curled on the adaxial side, and the fruits were seriously aborted after artificial pollination. Microscopic observation showed that the epidermal cells of petals, leaves, and seed coats of transgenic plants became smaller. The transcriptional levels of endogenous genes, such as NtCYCDs, NtSTM, NtKNAT1, and NtASs, were upregulated by PsnCYCD1;1. Therefore, PsnCYCD1;1 gene played an important role in the regulation of flower organ and stem development, providing new understanding for the functional characterization of CYCD gene and new resources for improving the ornamental value of horticultural plants.

4.
Int J Mol Sci ; 22(11)2021 May 29.
Article in English | MEDLINE | ID: mdl-34072501

ABSTRACT

d-type cyclins (CYCDs) are a special class of cyclins and play extremely important roles in plant growth and development. In the plant kingdom, most of the existing studies on CYCDs have been done on herbaceous plants, with few on perennial woody plants. Here, we identified a Populus d-type cyclin gene, PsnCYCD1;1, which is mainly transcribed in leaf buds and stems. The promoter of PsnCYCD1;1 activated GUS gene expression and transgenic Arabidopsis lines were strongly GUS stained in whole seedlings and mature anthers. Moreover, subcellular localization analysis showed the fluorescence signal of PsnCYCD1;1-GFP fusion protein is present in the nucleus. Furthermore, overexpression of the PsnCYCD1;1 gene in Arabidopsis can promote cell division and lead to small cell generation and cytokinin response, resulting in curved leaves and twisted inflorescence stems. Moreover, the transcriptional levels of endogenous genes, such as ASs, KNATs, EXP10, and PHB, were upregulated by PsnCYCD1;1. Together, our results indicated that PsnCYCD1;1 participates in cell division by cytokinin response, providing new information on controlling plant architecture in woody plants.


Subject(s)
Arabidopsis Proteins/genetics , Arabidopsis/genetics , Cell Division/genetics , Cyclin D3/genetics , Gene Expression , Plant Leaves/genetics , Populus/genetics , Arabidopsis Proteins/metabolism , Cloning, Molecular , Cyclin D3/metabolism , Gene Expression Regulation, Plant , Morphogenesis/genetics , Organ Specificity , Phenotype , Plant Leaves/metabolism , Plant Proteins/genetics , Plants, Genetically Modified , Protein Transport
5.
Article in English | MEDLINE | ID: mdl-31540280

ABSTRACT

The Huang-huai-hai River Basin is one of the most economically developed areas, but is also heavily impacted by drought and flood disasters. Research on the precipitation feature of the Huang-huai-hai River Basin is of great importance to the further discussion of the cause of flood disaster. Based on the selected meteorological stations of the study area from 1961-2016, the inverse distance weighting method was used to get daily precipitation grid data. Interannual variation of precipitation intensity and cover area of different precipitation classes was analyzed. The generalized extreme-value distribution method was used to analyze the spatial distribution of extreme precipitation. The results show that: (1) decrease of accumulated precipitation in light precipitation year and moderate precipitation year might be the reason why the precipitation in the whole basin decreased, but the coefficient of variation (CV) of different classes of precipitation and precipitation days does not change significantly; (2) since the cover area of precipitation > 50 mm and precipitation intensity both decreased, the extreme precipitation of the whole basin may be decreasing; (3) extreme precipitation mainly occurred in the loess plateau in the northeast of Huang-huai-hai River Basin, Dabieshan in the middle of Huang-huai-hai River Basin and other areas.


Subject(s)
Climatic Processes , China , History, 20th Century , History, 21st Century , Rivers
6.
Environ Manage ; 57(6): 1188-203, 2016 Jun.
Article in English | MEDLINE | ID: mdl-26908125

ABSTRACT

Coal power generation capacity is expanding rapidly in the arid northwest regions in China. Its impact on water resources is attracting growing concerns from policy-makers, researchers, as well as mass media. This paper briefly describes the situation of electricity-water conflict in China and provides a comprehensive review on a variety of water resources management policies in China's coal power industry. These policies range from mandatory regulations to incentive-based instruments, covering water withdrawal standards, technological requirements on water saving, unconventional water resources utilization (such as reclaimed municipal wastewater, seawater, and mine water), water resources fee, and water permit transfer. Implementing these policies jointly is of crucial importance for alleviating the water stress from the expanding coal power industry in China.


Subject(s)
Coal , Power Plants , Water Resources/supply & distribution , Water Supply/methods , China , Industry/standards , Power Plants/standards , Seawater/chemistry , Wastewater/chemistry , Wastewater/economics , Water Purification/methods , Water Purification/standards , Water Supply/economics , Water Supply/standards
7.
Environ Sci Technol ; 48(19): 11082-9, 2014 Oct 07.
Article in English | MEDLINE | ID: mdl-25215622

ABSTRACT

The energy sector is increasingly facing water scarcity constraints in many regions around the globe, especially in China, where the unprecedented large-scale construction of coal-fired thermal power plants is taking place in its extremely arid northwest regions. As a response to water scarcity, air-cooled coal power plants have experienced dramatic diffusion in China since the middle 2000s. By the end of 2012, air-cooled coal-fired thermal power plants in China amounted to 112 GW, making up 14% of China's thermal power generation capacity. But the water conservation benefit of air-cooled units is achieved at the cost of lower thermal efficiency and consequently higher carbon emission intensity. We estimate that in 2012 the deployment of air-cooled units contributed an additional 24.3-31.9 million tonnes of CO2 emissions (equivalent to 0.7-1.0% of the total CO2 emissions by China's electric power sector), while saving 832-942 million m(3) of consumptive water use (about 60% of the total annual water use of Beijing) when compared to a scenario with water-cooled plants. Additional CO2 emissions from air-cooled plants largely offset the CO2 emissions reduction benefits from Chinese policies of retiring small and outdated coal plants. This water-carbon trade-off is poised to become even more significant by 2020, as air-cooled units are expected to grow by a factor of 2-260 GW, accounting for 22% of China's total coal-fired power generation capacity.


Subject(s)
Carbon/analysis , Coal , Industry , Power Plants , Water/analysis , Air , Air Pollutants/analysis , Air Pollution/analysis , Carbon Dioxide/analysis , China , Diffusion , Fresh Water , Geography , Models, Theoretical , Water Supply
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