[Detection of the etiologic agent of hemorrhagic fever with a renal syndrome in the lungs of wild rodents]. / Obnaruzhenie étiologicheskogo agenta gemorragicheskogo likhoradki s pochechnym sindromom v legkikh dikikh gryzunov.

The results of the studies on wild rodents trapped in the areas of the Leningrad region visits to which were associated with human cases of hemorrhagic fever with renal syndrome (HFRS) are presented. In one of these areas 30% of bank voles were found to be infected. Specific fluorescence of high intensity was found in ctyostat sections of lungs from bank voles treated by the indirect fluorescent antibody procedure. Fluorescent granules, their clumps and large conglomerates were localized in the cytoplasm of alveolar epithelium cells. The antigen-containing cells formed foci within which 70% or more elements showed fluorescence or were found diffusely throughout the section (dozens of infected cells in each field of vision). The antigen-containing lung sections were used for the indirect immunofluorescence test with sera from the subjects clinically diagnosed as having HFRS. Positive results in titers ranging from 1 : 10 to 1 : 1280 were obtained in 6 out of 7 subjects examined. Examinations of paired sera from one of them revealed an over 100-fold rise in titers during the 3rd-7th week after the onset of the disease.

[Comparative study of different methods of determining ovalbumin in preparations of purified influenza virus]. / Sravnitel'noe izuchenie razlichnykh metodov opredeleniia oval'bumina v preparatakh ochishchennogo virusa grippa.

The degree of purity of inactivated influenza vaccines was assayed in the anaphylaxis test in guinea pigs as well as by the values of total protein and ovalbumin measured by IHA and ID tests. Quantitation of ovalbumin in vaccines by the IHA test with an antiovalbumin diagnostic preparation was found to be most informative, rapid and highly sensitive. This method may be recommended for quantitation of ovalbumin impurities in samples of purified influenza virus obtained by different methods and for different purposes.