ABSTRACT
To promote the efficient utilization of China's reuse water resources and optimize the allocation of water resources, an analysis of factors influencing the development and utilization of reuse water resources was conducted. The uniqueness and competitiveness of reuse water resources were analyzed, and the driving and constraint mechanisms were revealed. A potential indicator system for the bilateral coordination of the supply and demand of reuse water was also established. Based on redundancy analysis (RDA), key indicators for the prediction of reuse water development and utilization potential were screened. On this basis, a national-scale reuse water development and utilization potential prediction model was constructed (the random effects model, REM). Given some uncertainty in the parameters of the REM model, the confidence interval ranges of the parameters at the 10%-90% quartile levels were identified. The results show that four indicators (ecological water consumption, density of water supply pipelines in built-up areas, fixed asset investment in the construction of reuse water treatment facilities, and total wastewater treatment) are closely related to the development and utilization of reuse water and, hence, are key indicators. The REM for the potential prediction has a high fitting accuracy, which can effectively reflect the fluctuations in the observed values with a maximum fitting error of -8.5%. China's reuse water development and utilization will continue to maintain rapid growth long into the future, reaching 12.9 billion m3 by 2025. This will help optimize national urban water supply structures and improve the reuse rate of regional water resources.
ABSTRACT
OBJECTIVE: To explore the effects of NF-E2-related factor-2 (NRF2)-617C/A promoter polymorphism on NRF2 expression as well as lipopolysaccharide-induced inflammatory responses in macrophages. METHODS: NRF2-617C/A promoter fragments were synthesized by chemical method and cloned into a pUC57 vector. The dul-luciferase reporter assay was employed to determine the activity of promoters. Then recombinant adenoviral vectors were constructed and transfected into macrophages. The expression of Nrf2 was examined by Western blotting and reverse transcription (RT)-PCR. The expressions of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL-6) and interleukin-10 (IL-10) in macrophages after the stimulation of LPS were determined by enzyme-linked immunosorbent assay (ELISA). RESULTS: The activity of NRF2-617C promoter-luciferase reporter (FLuc/RLuc activity ratio) was significantly higher than that of NRF2-617A group (0.584 ± 0.016 vs 0.258 ± 0.018, P < 0.05).The NRF2 protein and mRNA levels in -617C group were much higher than those of 617A group (1.123 ± 0.080 vs 0.951 ± 0.057,1.889 ± 0.031 vs 1.647 ± 0.323, both P < 0.05). After the stimulation of LPS, the NRF2 protein expression in macrophages significantly increased (0.584 ± 0.016 vs 0.258 ± 0.018, P < 0.05). Compared with -617A group, there was a significantly higher expression of NRF2 in -617C group (0.671 ± 0.033 vs 0.751 ± 0.014, P < 0.05). Additionally, the productions of IL-6 and IL-10 in -617C group were markedly lower than those in -617A group as well as IL-6/IL-10 (both P < 0.05). However, no significant difference existed in the levels of TNF-α between -617C and -617A groups (P > 0.05). CONCLUSIONS: The -617C/A promoter polymorphism of NRF2 may influence the NRF2 expression. And it appears to be associated with the LPS-induced inflammatory responses in macrophages.