[Clinical research progress in the treatment of intrabony defect by minimally invasive periodontal surgery].

Minimally invasive surgery develops rapidly in the periodontal treatments, especially in the periodontal regenerative treatment, in recent years. It supplements, to a certain extent, the insufficiency of the conventional periodontal regenerative treatment. The minimally invasive surgery has many advantages such as enhancing the healing process, reducing surgical chair time and minimizing patient discomfort, etc. It has been proved to improve the clinical effect and provide additional benefits compared to conventional approaches. At present, there are many studies on minimally invasive techniques used in tooth extraction or implant placement in China, but there are few reports on the application of periodontal minimally invasive surgical techniques. Thus based on the reviews of the literatures, this article describes the applications, advantages, indications, microsurgical instruments of minimally invasive periodontal surgery on the treatment of intrabony defect, including various minimally invasive surgical procedures. The review also demonstrates the therapeutic effects and research progress of minimally invasive periodontal surgery combined with biomaterials used in the treatments of intrabony defect. The present article may also provide reference for clinicians applying minimally invasive surgeries to treat intrabony defects.

[Effect of neoadjuvant chemotherapy on the overall treatment strategy in operable breast cancer].

The intention of neoadjuvant chemotherapy in breast cancer is to shrink the tumors in locally advanced disease and to improve the degree of cure of operation (security). Therefore, it is expected to improve quality of life and survival for patients. Additionally, neoadjuvant chemotherapy is administered based on the observable primary tumor. Thus, the timely assessment of tumor response to chemotherapeutic drugs provides a basis for subsequent treatment. Currently, however, the treatment concept of breast cancer requires whole process management. It requires clinicians to develop the overall treatment strategy according to tumor biological information of patients, as well as timely and reasonable adjustment of the subsequent treatment based on the response to neoadjuvant chemotherapy. These are new problems arising from neoadjuvant chemotherapy.

SCC-112 gene is involved in tumor progression and promotes the cell proliferation in G2/M phase.

PURPOSE: SCC-112 is a novel cell cycle-related gene and differentially expressed in cancers. Suggesting the complex role of SCC-112 might be existent in cell proliferation and tumor development. The relative research on SCC-112 has been few so far. This study is attempted to explore the role of SCC-112 in tumorigenesis. EXPERIMENTAL DESIGN: RT-PCR and western blot were performed on seven tumor-normal paired tissues and nine cell lines. Immunohistochemistry was carried out for analyzing the expression of SCC-112 in nasopharyngeal tissues. 293T and three nasopharyngeal cell lines were transfected with expression vector (pCMV-SPORT6-SCC-112) or its siRNA. Cell proliferation was examined by MTT and clone formation experiments. Immunoprecipitation determined the interacted protein of SCC-112, and FACS detected cell cycle parameter on cells treated with synchronized reagent. RESULTS: SCC-112 ( approximately 150 kDa) is up-regulated in tumor tissue as compared to the corresponding normal tissue and was detected in the tested cell lines. Overexpression of SCC-112 ( approximately 150 kDa) in 293T and three nasopharyngeal cell lines promoted cell proliferation and clone formation while downregulation of SCC-112 ( approximately 150 kDa) in these cells resulted in the opposite. Moreover, SCC-112 was found to interact with p63 and overexpression of SCC-112 up-regulated p63 expression. SCC-112 expression level positively correlated with cells in G2/M phase. CONCLUSIONS: These findings suggest that SCC-112 improve cell proliferation and contributes to tumorigenesis by interacting with p63 and promoting cell cycling. SCC-112 might be an alternative target in tumor biomarking and mechanistic investigation.

[Effect of zhuhuang frost on wound hydroxy-proline level after anal operation].

OBJECTIVE: To observe the effect of Zhuhuang Frost (ZHF), an external preparation of Chinese herbal medicine, on level of hydroxy-proline (Hyp) in surgical wound after anal operation. METHODS: Fifty postoperational patients after low position simple operation of anal fistula were randomly divided into two groups and treated with ZHF and Mayinglong Musk hemorrhoidal paste (MMHP) respectively. The level of Hyp in granulation of wound was tested using alkaline hydrolysis assay at the 3rd, 7th and 14th day after operation respectively. RESULTS: The Hyp level tested at the 7th and 14th day after operation in the ZHF treated group was higher than that in the MMHP treated group, but those tested at the 3rd postoperational day in the two groups was not different significantly. CONCLUSION: ZHF could increase the Hyp level in postoperational granulation of wound after anal operation.

What is the significance of nasal involvement in nasopharyngeal carcinoma?

PURPOSE: The purpose of this study was to differentiate the patterns of nasal fossa involvement in nasopharyngeal carcinoma (NPC) and to clarify its prognostic influence on local control and survival after radiation therapy. METHODS AND MATERIALS: Between November 1989 and July 1991, 218 patients with histologically proven local-regional NPC were treated with radiotherapy following the protocol at the Department of Radiation Oncology, Cancer Hospital, Shantou University School of Medicine. All patients had pretreatment CT scans. Fiberoptic endoscopic examination was performed every week during treatment and at the time of every follow-up visit to define the initial extent of disease and to evaluate treatment response. No chemotherapy or brachytherapy was given. RESULTS: Of the 218 patients, 87 had nasal involvement. Sixty of them had a pattern of mucosal infiltration (MI), another 27 had an exophytic protruding (EP) component. The likelihood of residual disease after irradiation, the local relapse rate, 5-year freedom from progression rate (FFP), and death rate associated with local relapse (DRALR) of MI and EP were 36.7% vs. 3.7%, 30.0% vs. 7.4%, 26.7% vs. 51.8%, and 25.0% vs. 3.7% with p<0.004, p<0.005, p<0.02, and p<0.03, respectively. Multivariate analysis in this selected group demonstrated that infiltration of nasal fossa mucosa was an independent prognostic factor on primary control and freedom from progression. CONCLUSION: Differentiation of nasal fossa involvement according to MI or EP is of value in predicting the outcome of treatment. We suggest that only the MI group should be considered as nasal involvement in the staging of NPC.

Studies into the effect of tyrosine phosphatase inhibitor phenylarsine oxide on NFkappaB activation in T lymphocytes during aging: evidence for altered IkappaB-alpha phosphorylation and degradation.

Nuclear Factor kappa B (NFkappaB) is a critical regulator of several genes involved in immune and inflammatory responses. Treatment of T cells with a variety of stimuli, including TNF-alpha, leads to the translocation of the active p65-50 heterodimer to the nucleus, albeit at a lower level in T cells from the elderly. We demonstrate here that pretreatment with PAO results in the inhibition of NFkappaB induction in TNF-alpha treated T cells, suggesting a role for PAO-sensitive phosphatase in the activation of the NFkappaB via this pathway in human T cells. Furthermore, it demonstrates that aging does not influence the sensitivity of this phosphatase. Treatment with DMP prior to treatment with PAO and TNF abolishes the inhibition induced by PAO, in T cells from both young and old donors, alike. Finally, we demonstrate that a failure to degrade IkappaB-alpha in cytosols of TNF-treated T cells pretreated with PAO is due to its interference with the phosphorylation of IkappaB-alpha and not due to its inhibitory effect on proteasomal degradation. These data collectively suggest that PAO interferes with the phosphorylation and the regulated degradation of IkappaB-alpha, induced by TNF, without affecting the chymotryptic activity of the proteasome, independent of age.