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1.
Sichuan Da Xue Xue Bao Yi Xue Ban ; 49(1): 39-43, 2018 Jan.
Article in Chinese | MEDLINE | ID: mdl-29737087

ABSTRACT

OBJECTIVE: To investigate the expressions of nerve growth factor (NGF) and acid-sensing ion channel 3 (ASIC3) in prostatic tissue of experimental rats with type Ⅲ prostatitis. METHODS: Thirty SD rats were randomly allocated into control group and experimental group. The rats in control group were subjected to pelvic and bilateral scapular subcutaneous injections of 0.9% sodium chloride,while the rats in experimental group were given pelvic and bilateral scapular subcutaneous injections of mixed suspension of complete Freund's adjuvant and prostatic tissue to induce autoimmune prostatitis (EAP).Tactile allodynia was quantified using Von-Frey as a measure of pelvic pain behavior. This measurement was performed on 0th,5th,10th,20th,30th and 40th day in the two groups. After that,the prostate samples were collected and processed for HE staining,while the expressions of NGF and ASIC3 were measured by immunohistochemistry and Western blot. RESULTS: Von-Frey filaments measurement showed that pelvis pain in EAP group was significantly more obvious than that in control group. HE staining found lymphocytes and neutrophils infiltrated in the prostate of EAP rats,but no inflammatory cells in the prostate of control group rats. The expressions of NGF and ASIC3 were significantly increased in EAP group when compared with control group ( P<0.01). CONCLUSION: The expressions of NGF and ASIC3 in the prostate with EAP were significantly increased,which may be the important mediators of chronic pelvic pain.


Subject(s)
Acid Sensing Ion Channels/metabolism , Nerve Growth Factor/metabolism , Prostatitis/metabolism , Animals , Disease Models, Animal , Male , Pelvic Pain , Random Allocation , Rats , Rats, Sprague-Dawley
2.
Exp Ther Med ; 14(1): 51-58, 2017 Jul.
Article in English | MEDLINE | ID: mdl-28672892

ABSTRACT

The present study aimed to determine the expression and mediation of interleukin-17 (IL-17) and chemokine ligand 2 (CCL2) in a rat model with experimental autoimmune prostatitis (EAP). A total of 44 Sprague Dawley (SD) rats were used in the present study. Of these, a total of 20 two-month-old SD rats were randomly divided into a normal control (n=10) and a model group (EAP group, n=10). The remaining 24 two-month old SD rats were treated in the same way as EAP rats and subsequently randomly divided into a tacrolimus group (n=8), a celecoxib group (n=8) and a normal saline (NS) control group (n=8). Rats in the EAP and normal control groups underwent the Von Frey filaments behavioral test; rats in the tacrolimus, celecoxib and normal saline groups received a pain test following intervention treatment. Prostate tissues of SD rats in each group were harvested for reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blot analysis to observe the expression of IL-17 and CCL2. In the pain-reaction test, the occurrence of abnormal pain in the EAP group was significantly higher compared with the control group (P<0.001). The celecoxib group experienced a significant decrease in pain at day 10 compared with the NS group (P<0.01), while the decrease in pain experienced by the tacrolimus group was only significant at day 30 (P<0.001) and the pain experienced by the NS group decreased slightly over this same period. Results of RT-qPCR and western blot analysis indicated that, compared with the control group, the expression of IL-17 and CCL2 in the prostate tissue of EAP rats was significantly upregulated 50 days following modeling (P<0.05). On day 30 following intervention, the expression of IL-17 and CCL2 in the prostate of rats in the tacrolimus and celecoxib groups was significantly downregulated compared with the NS group (P<0.05). Therefore, the results of the current study demonstrate that IL-17 and CCL2 serve a vital role in the morbidity of the experimental autoimmune prostatitis and may also have a mediation effect on pelvic pain associated with chronic prostatitis.

3.
Zhonghua Nan Ke Xue ; 23(5): 399-405, 2017 May.
Article in Chinese | MEDLINE | ID: mdl-29717828

ABSTRACT

OBJECTIVE: To investigate the role of mast cells in chronic prostatitis / chronic pelvic pain syndrome (CP/CPPS). METHODS: Forty-five male SD rats were equally randomized into a control, an experimental autoimmune prostatitis (EAP) model, and an intervention group. The EAP model was made in the latter two groups by subcutaneous injection of mixed suspension of complete Freund's adjuvant and prostate tissue, while the controls were treated subcutaneously with 0.9% sodium chloride. Tactile allodynia was quantified in the pelvic region of the control and EAP animals using Von-Frey filaments at 5, 10, 20, 30 and 40 days. After successful establishment of the EAP model, the rats of the intervention group were injected intraperitonieally with cromolyn sodium for 10 days, and meanwhile tactile allodynia was detected in the rats of the intervention and EAP model groups every other day. Then the prostates of the rats were harvested for HE and toluidine blue staining and measurement of the expression of mast cell tryptase by immunohistochemistry and Western blot. RESULTS: Von-Frey assessment showed a more severe pelvic pain in the EAP model than in the control rats, but milder in the intervention group than in the EAP models. HE staining revealed infiltration of lymphocytes and neutrophils in the prostate and congestion surrounding the gland in the EAP model rats, but none in the controls. However, both the infiltration and congestion were significantly alleviated in the intervention group. Toluidine blue staining shown that. Compared with the control group, the total count of mast cells and the number degranulated mast cells were markedly increased in the EAP models (P <0.01) but decreased in the intervention group (P <0.05). Both immunohistochemistry and Western blot manifested that the expression of tryptase in the mast cells was remarkably upregulated in the EAP (both P <0.01) but down-regulated in the intervention group (P <0.05 and P <0.01). CONCLUSIONS: Both the total count of mast cells and the number of degranulated mast cells are significantly increased in the prostate of EAP rats. Mast cells are one of the most important mediators of type Ⅲ prostatitis-induced chronic pelvic pain, which can be used as a target for the intervention and treatment of type Ⅲ prostatitis.


Subject(s)
Autoimmune Diseases/etiology , Mast Cells/physiology , Prostatitis/etiology , Adjuvants, Immunologic , Animals , Autoimmune Diseases/pathology , Cell Degranulation , Chronic Disease , Chronic Pain/etiology , Disease Models, Animal , Freund's Adjuvant , Male , Mast Cells/enzymology , Pelvic Pain/etiology , Prostatitis/pathology , Random Allocation , Rats , Rats, Sprague-Dawley , Tryptases/metabolism
4.
Biochem Biophys Res Commun ; 483(1): 669-673, 2017 01 29.
Article in English | MEDLINE | ID: mdl-27988336

ABSTRACT

IgE is a key effector molecule in atopic diseases; however, the regulation mechanisms of IgE production in IgE B cells remain poorly understood. In the present study, we demonstrate that JSI-124 (cucurbitacin I), a selective STAT3 inhibitor, selectively inhibits production of IgE by a human IgE B cell line, CRL-8033 cells, while does not affect the IgG production by IgG B cell lines. In the aspect of molecular mechanism, we found that Igλ, but not Ighe, gene expression was suppressed by JSI-124. The above effects of JSI-124 were not mediated by affecting cellular proliferation or apoptosis. Furthermore, multiple B cell differentiation-related genes expression was not significantly affected by JSI-124. Taken together, we demonstrate a potential strategy of therapeutically suppressing IgE production without affecting IgG production in atopic patients.


Subject(s)
B-Lymphocytes/drug effects , Immunoglobulin E/biosynthesis , Triterpenes/pharmacology , Apoptosis/drug effects , B-Lymphocytes/immunology , Cell Differentiation/drug effects , Cell Line , Cell Proliferation , Gene Expression/drug effects , Genes, Immunoglobulin/drug effects , Humans , Immunoglobulin E/genetics , Immunoglobulin G/biosynthesis , Immunoglobulin G/genetics , STAT3 Transcription Factor/antagonists & inhibitors
5.
Mol Med Rep ; 15(1): 375-379, 2017 Jan.
Article in English | MEDLINE | ID: mdl-27959435

ABSTRACT

Previous studies have demonstrated that oridonin, a tetracyclic diterpenoid compound extracted from Rabdosia rubescens, inhibits proliferation and induces apoptosis in several tumor cell lines. However, the mechanism by which oridonin inhibits the cell cycle remains poorly understood. In the present study, possible mechanisms by which oridonin affects cell cycle progression were explored in A549 lung cancer cells. Flow cytometry analysis indicated that oridonin inhibited the proliferation of A549 cells by inducing G2/M cell cycle arrest in a dose­dependent manner. Western blot analysis revealed that in oridonin treated cells, phosphorylated (p­)ATM serine/threonine kinase (S1981), p­checkpoint kinase 2 (CHK2) (T68), p­p53, and phosphorylated H2A histone family member X protein levels were visibly increased, indicating that oridonin promoted G2/M arrest in A549 cells through the ATM­p53­CHK2 pathway. This data suggests that oridonin promotes G2/M arrest in A549 cells by facilitating ATM activation, which is likely a common mechanism in other tumor cell types when using this drug for cancer treatment.


Subject(s)
Antineoplastic Agents, Phytogenic/pharmacology , Ataxia Telangiectasia Mutated Proteins/metabolism , Diterpenes, Kaurane/pharmacology , Enzyme Activation/drug effects , G2 Phase Cell Cycle Checkpoints/drug effects , Lung Neoplasms/drug therapy , M Phase Cell Cycle Checkpoints/drug effects , A549 Cells , Antineoplastic Agents, Phytogenic/chemistry , Apoptosis/drug effects , Cell Proliferation/drug effects , Diterpenes, Kaurane/chemistry , Humans , Isodon/chemistry , Lung/drug effects , Lung/metabolism , Lung/pathology , Lung Neoplasms/metabolism , Lung Neoplasms/pathology , Phosphorylation/drug effects , Signal Transduction/drug effects
6.
Oncotarget ; 7(50): 83115-83133, 2016 Dec 13.
Article in English | MEDLINE | ID: mdl-27825118

ABSTRACT

Evidence supports the upregulation of MUC1 in prostate cancer (PC). However, this has not been thoroughly investigated. We report here an association of MUC1 upregulation with PC metastasis and the development of castration resistant PC (CRPC). MUC1 expression was specifically increased in DU145 cell-derived PC stem-like cells (PCSLCs) in comparison to their non-PCSLCs counterparts. While immunohistochemistry staining of 34 primary PCs revealed variability in MUC1 expression, Nanostring technology demonstrated elevated MUC1 mRNA levels in 4 of 7 PCs compared to their normal matched tissues. By analyzing MUC1 mRNA levels and gene copy number (GCN) using the OncomineTM database, elevations in MUC1 mRNA in 82 metastases versus 280 primary PCs and in MUC1 GCN in 37 metastases over 181 primary tumors were demonstrated. Analysis of genomic datasets within cBioPortal revealed increases in MUC1 GCN in 2% (6/333) of primary PCs, 6% (9/150) of metastatic PCs, and 33% (27/82) of CRPCs; in comparison, the respective increase in androgen receptor (AR) GCN was 1%, 63%, and 56%, revealing a specific increase in MUC1 GCN for CRPC. Furthermore, a 25-gene MUC1 network was amplified in 52% of CRPCs compared to 69% of CRPCs displaying increases in an AR co-regulator group. While genomic alterations in the MUC1 network largely overlap with those in the AR group, 18 CRPCs (66.7% being neuroendocrine PC) showed genomic alterations only in the MUC1 network. Moreover, genomic alterations in the MUC1 network correlated with PC relapse. Collectively, our observations suggest a combination therapy involving MUC1-based immunotherapy and androgen deprivation.


Subject(s)
Adenocarcinoma/genetics , Biomarkers, Tumor/genetics , Gene Amplification , Mucin-1/genetics , Neuroendocrine Tumors/genetics , Prostatic Neoplasms, Castration-Resistant/genetics , Prostatic Neoplasms/genetics , Adenocarcinoma/metabolism , Adenocarcinoma/secondary , Adenocarcinoma/therapy , Aged , Aged, 80 and over , Animals , Antineoplastic Agents, Phytogenic/pharmacology , Biomarkers, Tumor/metabolism , Cell Line, Tumor , Cell Movement/drug effects , Computational Biology , Databases, Genetic , Disease Progression , Disease-Free Survival , Docetaxel , Gene Dosage , Gene Expression Regulation, Neoplastic , Gene Regulatory Networks , Humans , Male , Mice, Inbred NOD , Mice, SCID , Middle Aged , Mucin-1/metabolism , Neoplastic Stem Cells/drug effects , Neoplastic Stem Cells/metabolism , Neoplastic Stem Cells/pathology , Neuroendocrine Tumors/metabolism , Neuroendocrine Tumors/secondary , Neuroendocrine Tumors/therapy , Prostatic Neoplasms/metabolism , Prostatic Neoplasms/pathology , Prostatic Neoplasms/therapy , Prostatic Neoplasms, Castration-Resistant/metabolism , Prostatic Neoplasms, Castration-Resistant/pathology , Prostatic Neoplasms, Castration-Resistant/therapy , RNA, Messenger/genetics , RNA, Messenger/metabolism , Receptors, Androgen/metabolism , Signal Transduction , Survival Analysis , Taxoids/pharmacology , Time Factors , Up-Regulation , Xenograft Model Antitumor Assays
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