Telomerase-related advances in hepatocellular carcinoma: A bibliometric and visual analysis.

BACKGROUND: As a critical early event in hepatocellular carcinogenesis, telomerase activation might be a promising and critical biomarker for hepatocellular carcinoma (HCC) patients, and its function in the genesis and treatment of HCC has gained much attention over the past two decades. AIM: To perform a bibliometric analysis to systematically assess the current state of research on HCC-related telomerase. METHODS: The Web of Science Core Collection and PubMed were systematically searched to retrieve publications pertaining to HCC/telomerase limited to "articles" and "reviews" published in English. A total of 873 relevant publications related to HCC and telomerase were identified. We employed the Bibliometrix package in R to extract and analyze the fundamental information of the publications, such as the trends in the publications, citation counts, most prolific or influential writers, and most popular journals; to screen for keywords occurring at high frequency; and to draw collaboration and cluster analysis charts on the basis of coauthorship and co-occurrences. VOSviewer was utilized to compile and visualize the bibliometric data. RESULTS: A surge of 51 publications on HCC/telomerase research occurred in 2016, the most productive year from 1996 to 2023, accompanied by the peak citation count recorded in 2016. Up to December 2023, 35226 citations were made to all publications, an average of 46.6 citations to each paper. The United States received the most citations (n = 13531), followed by China (n = 7427) and Japan (n = 5754). In terms of national cooperation, China presented the highest centrality, its strongest bonds being to the United States and Japan. Among the 20 academic institutions with the most publications, ten came from China and the rest of Asia, though the University of Paris Cité, Public Assistance-Hospitals of Paris, and the National Institute of Health and Medical Research (INSERM) were the most prolific. As for individual contributions, Hisatomi H, Kaneko S, and Ide T were the three most prolific authors. Kaneko S ranked first by H-index, G-index, and overall publication count, while Zucman-Rossi J ranked first in citation count. The five most popular journals were the World Journal of Gastroenterology, Hepatology, Journal of Hepatology, Oncotarget, and Oncogene, while Nature Genetics, Hepatology, and Nature Reviews Disease Primers had the most citations. We extracted 2293 keywords from the publications, 120 of which appeared more than ten times. The most frequent were HCC, telomerase and human telomerase reverse transcriptase (hTERT). Keywords such as mutational landscape, TERT promoter mutations, landscape, risk, and prognosis were among the most common issues in this field in the last three years and may be topics for research in the coming years. CONCLUSION: Our bibliometric analysis provides a comprehensive overview of HCC/telomerase research and insights into promising upcoming research.

Autophagy and biotransformation affect sorafenib resistance in hepatocellular carcinoma.

As sorafenib is a first-line drug for treating advanced hepatocellular carcinoma, sorafenib resistance has historically attracted attention. However, most of this attention has been focused on a series of mechanisms related to drug resistance arising after sorafenib treatment. In this study, we used proteomic techniques to explore the potential mechanisms by which pretreatment factors affect sorafenib resistance. The degree of redundant pathway PI3K/AKT activation, biotransformation capacity, and autophagy level in hepatocellular carcinoma patients prior to sorafenib treatment might affect their sensitivity to sorafenib, in which ADH1A and STING1 are key molecules. These three factors could interact mechanistically to promote tumor cell survival, might be malignant features of tumor cells, and are associated with hepatocellular carcinoma prognosis. Our study suggests possible avenues of therapeutic intervention for patients with sorafenib-resistance and the potential application of immunotherapy with the aim of improving the survival of such patients.

Broadband photonic-assisted microwave receiver with high cross-channel interference suppression and image rejection.

A broadband photonic-assisted microwave receiver with high cross-channel interference suppression and image rejection is proposed and experimentally demonstrated. At the input of the microwave receiver, a microwave signal is injected into an optoelectronic oscillator (OEO), which functions as a local oscillator (LO) to generate a low-phase noise LO signal as well as a photonic-assisted mixer to down-convert the input microwave signal to the intermediate frequency (IF). A microwave photonic filter (MPF), realized by the joint operation of a phase modulator (PM) in the OEO and a Fabry-Perot laser diode (FPLD), is used as a narrowband filter to select the IF signal. Thanks to the wide bandwidth of the photonic-assisted mixer and the wide frequency tunable range of the OEO, the microwave receiver can support broadband operation. The high cross-channel interference suppression and image rejection are enabled by the narrowband MPF. The system is evaluated experimentally. A broadband operation from 11.27 to 20.85 GHz is demonstrated. For a multi-channel microwave signal with a channel spacing of 2 GHz, a cross-channel interference suppression ratio of 21.95 dB and an image rejection ratio of 21.51 dB are realized. The spurious-free dynamic range (SFDR) of the receiver is also measured to be 98.25 dB·Hz2/3. The performance of the microwave receiver for multi-channel communications is also experimentally evaluated.

Environmental estrogens shape disease susceptibility.

Along with industrialization, the environment is flooded with endocrine-disrupting chemicals, among which substances with estrogenic effects have attracted widespread attention in medical research. In terms of molecular mechanism, environmental estrogens can cause endocrine and metabolic disorders; interfere with multiple carcinogenic pathways; and lead to neurobehavioral disorders, reproductive toxicity, and multi- or trans-generational phenotypic abnormalities. However, many of the results from molecular and animal experiments were not supported by epidemiology, which may be related to the existence of a window of sensitivity to environmental estrogen exposure over the human life course, where the consequences of exposure vary greatly from other times. This paper will introduce the main sources of environmental estrogens, their toxicity and mechanisms of action, the status of research on several representative types, and current monitoring and treatment methods. We also discussed the extent of the risks to human health dialectically in the context of laboratory and epidemiological findings, with a view to better addressing these chemicals to which we are constantly exposed.

Microwave frequency measurement using a silicon integrated microring resonator.

Photonics-assisted instantaneous frequency measurement of a microwave signal using a silicon integrated microring resonator (MRR) is proposed and experimentally demonstrated. The frequency of a microwave signal has a unique relationship with the power ratio between the two microwave signals at the outputs of two microwave photonic filters (MPF) with complementary frequency responses. The key device to implement the MPFs is a silicon integrated MMR, which is employed to convert a phase-modulated optical signal to an intensity-modulated optical signal by placing two optical carriers at the complementary slopes of the MRR. For a given frequency measurement range and resolution, an MRR is designed and fabricated, and its use for instantaneous microwave frequency (IMF) measurement is implemented. For the fabricated MRR, an IMF measurement range of 14-25 GHz with a measurement accuracy of ±0.2GHz is achieved.

Parity-time-symmetric optoelectronic oscillator based on higher-order optical modulation.

An optoelectronic oscillator (OEO) for single-frequency microwave generation, enabled by broken parity time (PT) symmetry based on higher-order modulation using a Mach-Zehnder modulator, is proposed and demonstrated. Instead of using two physically separated mutually coupled loops with balanced gain and loss, the PT symmetry is realized using a single physical loop to implement two equivalent loops with the gain loop formed by the beating between the optical carrier and the ±1st-order sidebands and the loss loop formed by the beating between the ±1st-order sidebands and the ±2nd-order sidebands at a photodetector. The gain and loss coefficients are made identical in magnitude by controlling the incident light power to the modulator and the modulator bias voltage. Once the gain/loss coefficient is greater than the coupling coefficient, the PT symmetry is broken, and a single-frequency oscillation without using an ultra-narrow passband filter is achieved. The approach is evaluated experimentally. For an OEO with a loop length of 10.1 km, a single-frequency microwave signal at 9.997 GHz with a 55-dB sidemode suppression ratio and -142-dBc/Hz phase noise at a 10-kHz offset frequency is generated. No mode hopping is observed during a 5-hour measurement period.

Targeting tumor vascularization: promising strategies for vascular normalization.

Tumor recurrence after the clinical cure of tumor often results from the presence of an abnormal microenvironment, including an aberrant vasculature. The tumor microenvironment is rich in pro-angiogenic factors but lacks pro-maturation factors. Pro-angiogenic conditions in the tumor microenvironment, such as hypoxia, are double-edged swords, promoting both the repair of normal tissues and the development of an abnormal blood vessel network. The coexistence of perfusion and hypoxic zones and uneven blood vessel distribution in tumor tissues profoundly influence tumor deterioration, recurrence, and metastasis. Traditional anti-angiogenic therapies have shown limited efficacy, and promote drug resistance, and even metastasis. In contrast, vascular normalization therapy induces a more physiological-like state, leading to better outcomes and fewer side effects. Vascular normalization entails modifying the tumor vascular system to improve tumor oxygenation and substance transport, thereby contributing to improving the efficacy of radiotherapy, chemotherapy, and immunotherapy. This review mainly focuses on the process of tumor vascularization; potential therapeutic targets, including cells, metabolism, signaling pathways, and angiogenesis-related genes; and possible strategies to normalize blood vessels through regulating tumor vessel generation, the development of tumor vessels, and blood vessel fusion and pruning.

Circular RNA ITCH suppresses metastasis of gastric cancer via regulating miR-199a-5p/Klotho axis.

Circular RNAs (circRNAs) are considered as a new regulatory factor in growth, metastasis and therapeutic resistance of human cancers. But the clinical significance and underlying mechanism of circular RNA ITCH (circ-ITCH) in gastric cancer (GC) remain unknown. In the present study, we found that circ-ITCH was down-regulated in GC cell lines, GC tissues and their serum-derived exosomes. The level of circ-ITCH was related to invasion depth. Functional assays showed that circ-ITCH overexpression inhibited the proliferation, migration, invasion and epithelial mesenchymal transition (EMT) of GC cells, whereas circ-ITCH knockdown appeared an opposite effect. Bioinformatic analysis and luciferase reporter assay confirmed that circ-ITCH acted as miR-199a-5p sponge and increased the level of Klotho. The expression level of miR-199-5p was up-regulated in GC tissues and negatively correlated with that of circ-ITCH. MiR-199a-5p mimics reversed the effects on inhibiting metastasis induced by circ-ITCH overexpression and decreased the level of Klotho in GC cells. Our findings indicate that circ-ITCH suppresses metastasis of GC by acting as the sponge of miR-199a-5p and increasing Klotho expression, which serves as a potential biomarker and targets for the diagnosis and therapy of GC.Abbreviations: CircRNAs: circular RNAs; GC: gastric cancer; circ-ITCH: circular RNA Itchy E3 ubiquitin protein ligase; ceRNA: competitive endogenous RNA; EMT: Epithelial-mesenchymal transition; siRNA: Small interfering RNA; TEM: transmission electron microscope; NTA: nanoparticle tracking analysis.

Performance analysis of a LDPC coded OAM-based UCA FSO system exploring linear equalization with channel estimation over atmospheric turbulence: comment.

In a previously published paper [Opt. Express26(17), 22182 (2018)], the performance of a LDPC coded OAM-based UCA FSO system exploring linear equalization with channel estimation over atmospheric turbulence has been analyzed. We find that some concepts and descriptions in [Opt. Express26(17), 22182 (2018)] are inconsistent and paradoxical. In this comment, we point out the referred inconsistency and paradox one by one and present the correct explanations.

Linearized fiber-optic link with a high spurious free dynamic range over a wide frequency range.

An all-optical linearized fiber-optic link is presented. It solves the problem in most reported structures where a high spurious free dynamic range (SFDR) can only be obtained in a limited frequency range. The link only involves a laser, an optical modulator, and a photodetector. The novel design in the modulator bias setting enables the third-order intermodulation distortion to be suppressed by controlling only one bias voltage of a dual-parallel Mach-Zehnder modulator (DPMZM) while the other two bias voltages are set to bias the sub-MZM and the main MZM of the DPMZM at the standard peak and null point, respectively. The link has a very simple structure and does not require any electrical component, and hence a high SFDR can be obtained over a wide frequency range. Techniques are proposed using off-the-shelf components for stabilizing the modulator bias setting to maintain high SFDR performance when the link is operated in practice. Measured results demonstrate a high SFDR of 120.5 dB⋅Hz4/5±1.6 dB over a 2-20 GHz frequency range in an unamplified linearized fiber-optic link. To our knowledge, this is the first report of a linearized fiber-optic link with around 120 dB⋅Hz4/5 over such a wide frequency range.

Broadband high dynamic range fiber optic link based on a dual-polarization modulator.

This paper presents a simple, linearized fiber-optic link that is capable of realizing a high spurious free dynamic range (SFDR) at different input RF signal frequencies without the need of readjusting system parameters. The link is based on a commercial dual-polarization modulator followed by a linear polarizer. The third-order nonlinearity at the third-order intermodulation distortion frequency can be cancelled by designing the angle of the linear polarizer. No electrical component is involved in the linearization process. The high SFDR performance is theoretically analyzed, simulated using photonic simulation software, and experimentally verified. Experimental verification of the dual-polarization modulator-based linearized fiber-optic link shows that a high SFDR of more than 124 dB⋅Hz4/5 is obtained at different input RF signal frequencies over a 2-18 GHz bandwidth. An SFDR of 127.3 dB⋅Hz4/5 is also demonstrated with the use of an optical amplifier to increase the link output average optical power, which is among the highest reported SFDRs measured in a fiber-optic link.