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1.
Zhonghua Gan Zang Bing Za Zhi ; 24(3): 175-80, 2016 Mar 20.
Article in Chinese | MEDLINE | ID: mdl-27095759

ABSTRACT

OBJECTIVE: To investigate the association between the dose and plasma concentration of ribavirin (RBV) and sustained virologic response (SVR) during the anti-hepatitis C virus (HCV) treatment with pegylated interferon-α-2b (PEG-IFN-α-2b) and RBV. METHODS: A total of 40 patients with chronic hepatitis C (CHC) who were treated with PEG-IFN-α-2b and RBV as the antiviral treatment were enrolled, and according to the therapeutic effect (SVR was defined as HCV RNA maintained below the lower limit of detection at 24 weeks after drug discontinuation in patients who achieved virologic response at the end of treatment, and recurrence was defined as HCV RNA turning positive), these patients were divided into SVR group (20 patients aged 19-55 years, including 10 male patients) and recurrence group (20 patients aged 21-76 years, including 12 male patients). The HPLC-MS/MS was used to measure the RBV plasma concentration at weeks 4, 12, 24, and 48 of treatment. The t-test and receiver operating characteristic (ROC) curve were used for statistical analysis. RESULTS: During the antiviral treatment, the dose of RBV showed a significant difference between the two groups (15.01 ± 1.21 mg/kg vs 10.28 ± 2.81 mg/kg,t= 6.908,P= 0.000). The area under the ROC curve reached 0.96 (95%CI0.00-1.00,P= 0.000), suggesting that the dose of RBV had a high value in predicting SVR. When the dose of RBV was higher than 13.05 mg/kg (sensitivity 100%; specificity 85%), the possibility of achieving SVR was also increased. The RBV plasma concentrations in the SVR group at weeks 4,12, 24, and 48 of treatment were 1 894.8 ± 740.7 ng/ml, 2 029.9 ± 547.7 ng/ml, 2 011.8 ± 354.2 ng/ml, and2 093.5 ± 540.3 ng/ml, respectively, and those in the recurrence group were 1 223.1 ± 722.7 ng/ml, 1 286.9±685.4 ng/ml, 1304.7 ± 692.0 ng/ml, and 1 221.3 ± 655.3 ng/ml, respectively. The RBV plasma concentration at each time point showed significant differences between the two groups (t= 2.903,P= 0.006;t= 3.787,P= 0.001;t= 4.068,P= 0.000;t= 4.593,P= 0.000). The results of ROC analysis showed that the areas under the ROC curve at weeks 4, 12, 24, and 48 of treatment were 0.76 (95%CI0.61-0.92,P= 0.005), 0.83 (95%CI0.68-0.97,P= 0.000), 0.83 (95%CI0.69-0.98,P= 0.000), and 0.86 (95%CI0.72-1.00,P= 0.000), respectively, suggesting that the RBV plasma concentration had a high value in predicting SVR. When the cut-off values of RBV plasma concentration at weeks 4, 12, 24, and 48 of treatment were higher than 1262.5 ng/ml (sensitivity 90%; specificity 60%), 1432 ng/ml (sensitivity 100%; specificity 65%), 1427 ng/ml (sensitivity 100%; specificity 65%), and 1610 ng/ml (sensitivity 95%; specificity 80%), respectively, there was a greater possibility of achieving SVR. CONCLUSION: During the antiviral treatment with PEG-IFN-α-2b and RBV, the dose and plasma concentration of RBV have a high value in predicting the recurrence of CHC and the possibility of SVR.


Subject(s)
Antiviral Agents/blood , Hepacivirus/drug effects , Hepatitis C, Chronic/drug therapy , Interferon-alpha/blood , Ribavirin/blood , Sustained Virologic Response , Antiviral Agents/administration & dosage , Antiviral Agents/therapeutic use , Drug Therapy, Combination , Genotype , Hepacivirus/genetics , Hepacivirus/isolation & purification , Hepatitis C, Chronic/blood , Hepatitis C, Chronic/genetics , Hepatitis C, Chronic/virology , Humans , Interferon alpha-2 , Interferon-alpha/administration & dosage , Interferon-alpha/therapeutic use , Polyethylene Glycols/administration & dosage , Polyethylene Glycols/therapeutic use , Recombinant Proteins/administration & dosage , Recombinant Proteins/blood , Recombinant Proteins/therapeutic use , Recurrence , Ribavirin/administration & dosage , Ribavirin/therapeutic use , Sensitivity and Specificity , Tandem Mass Spectrometry , Treatment Outcome , Viral Load
2.
Eur Rev Med Pharmacol Sci ; 17(12): 1611-9, 2013 Jun.
Article in English | MEDLINE | ID: mdl-23832727

ABSTRACT

BACKGROUND: Adipose-derived stem cells (ADSCs) are multipotent progenitors that can commit to osteoblast, chondrocyte, adipocyte and several other lineages. The proper utilization of stem cells for clinical application requires an integrated understanding of multiple signal inputs that control maintenance of stemness, proliferation and differentiation. MATERIALS AND METHODS: In this study, ADSCs in rat were isolated from subcutaneous tissues of abdomen and inguinal fat pads, purified and expanded in vitro. Bone morphogenetic protein 2, TGF-beta 1, SB203580 (P38 MAPK inhibitor), Noggin (BMP inhibitor) and SB431542 (TGF-beta inhibitor) were used for differentiation into osteoblasts. RESULTS: Both TGF-beta signaling pathway and p38 MAKP signaling pathway could affect the differential direction of the ADSCs. PCR assays indicated that both TGF-beta signaling pathway and p38 MAKP signaling pathway played a crucial roles in osteoblasts differentiation of the ADSCs, the members included Smad 1, Smad 5, Smad 8, P38, ASK1, MKK3, MKK6, Runx 2, collagen type 1, and osteopontin. CONCLUSIONS: This research provides a theoretical basis and experimental evidence for therapeutic application of rat ADSCs to treat bone injury.


Subject(s)
Adipose Tissue/cytology , Osteoblasts/cytology , Stem Cells/cytology , Transforming Growth Factor beta/metabolism , p38 Mitogen-Activated Protein Kinases/metabolism , Animals , Antigens, Surface/metabolism , Cell Differentiation/physiology , Gene Expression Profiling , Osteoblasts/metabolism , Rats , Signal Transduction
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