Application of machine learning techniques in the diagnostic approach of PTSD using MRI neuroimaging data: A systematic review.

Background: At present, the diagnosis of post-traumatic stress disorder（PTSD） mainly relies on clinical symptoms and psychological scales, and finding objective indicators that are helpful for diagnosis has always been a challenge in clinical practice and academic research. Neuroimaging is a useful and powerful tool for discovering the biomarkers of PTSD,especially functional MRI (fMRI), structural MRI (sMRI) and Diffusion Weighted Imaging（DTI）are the most commonly used technologies, which can provide multiple perspectives on brain function, structure and its connectivity. Machine learning (ML) is an emerging and potentially powerful method, which has aroused people's interest because it is used together with neuroimaging data to define brain structural and functional abnormalities related to diseases, and identify phenotypes, such as helping physicians make early diagnosis. Objectives: According to the Preferred Reporting Items for Systematic reviews and Meta-Analyses (PRISMA) declaration, a systematic review was conducted to assess its accuracy in distinguishing between PTSD patients, TEHC(Trauma-Exposed Healthy Controls), and HC(healthy controls). Methods: We searched PubMed, Embase, and Web of Science using common words for ML methods and PTSD until June 2023, with no language or time limits. This review includes 13 studies, with sensitivity, specificity, and accuracy taken from each publication or acquired directly from the authors. Results: All ML techniques have an diagnostic accuracy rate above 70%，and support vector machine（SVM） are the most commonly used techniques. This series of studies has revealed significant neurobiological differences in key brain regions among individuals with PTSD, TEHC, and HC. The connectivity patterns of regions such as the Insula and Amygdala hold particular significance in distinguishing these groups. TEHC exhibits more normal connectivity patterns compared to PTSD, providing valuable insights for the application of machine learning in PTSD diagnosis. Conclusion: In contrast to any currently available assessment and clinical diagnosis, ML techniques can be used as an effective and non-invasive support for early identification and detection of patients as well as for early screening of high-risk populations.

Brain Activation Evoked by Motor Imagery in Pediatric Patients with Complete Spinal Cord Injury.

BACKGROUND AND PURPOSE: Currently, there is no effective treatment for pediatric patients with complete spinal cord injury. Motor imagery has been proposed as an alternative to physical training for patients who are unable to move voluntarily. Our aim was to reveal the potential mechanism of motor imagery in the rehabilitation of pediatric complete spinal cord injury. MATERIALS AND METHODS: Twenty-six pediatric patients with complete spinal cord injury and 26 age- and sex-matched healthy children as healthy controls were recruited. All participants underwent the motor imagery task-related fMRI scans, and additional motor execution scans were performed only on healthy controls. First, we compared the brain-activation patterns between motor imagery and motor execution in healthy controls. Then, we compared the brain activation of motor imagery between the 2 groups and compared the brain activation of motor imagery in pediatric patients with complete spinal cord injury and that of motor execution in healthy controls. RESULTS: In healthy controls, compared with motor execution, motor imagery showed increased activation in the left inferior parietal lobule and decreased activation in the left supplementary motor area, paracentral lobule, middle cingulate cortex, and right insula. In addition, our results revealed that the 2 groups both activated the bilateral supplementary motor area, middle cingulate cortex and left inferior parietal lobule, and supramarginal gyrus during motor imagery. Compared with healthy controls, higher activation in the bilateral paracentral lobule, supplementary motor area, putamen, and cerebellar lobules III-V was detected in pediatric complete spinal cord injury during motor imagery, and the activation of these regions was even higher than that of healthy controls during motor execution. CONCLUSIONS: Our study demonstrated that part of the motor imagery network was functionally preserved in pediatric complete spinal cord injury and could be activated through motor imagery. In addition, higher-level activation in sensorimotor-related regions was also found in pediatric complete spinal cord injury during motor imagery. Our findings may provide a theoretic basis for the application of motor imagery training in pediatric complete spinal cord injury.

Impedance matching system using triple liquid stub tuners for high-power ion cyclotron resonance heating in EAST tokamak.

Ion cyclotron resonance heating (ICRH), one of the main auxiliary methods, for high-power and long-pulse plasma heating had been developed in Experimental Advanced Superconducting Tokamak (EAST). An impedance matching system, one important part of ICRH, had been developed for high-power injection and transmitter protection by reducing the reflected power from the antenna. The input impedance in the outlet of the stub tuner can be measured by voltage-current probes installed on the coaxial transmission line between the antenna and triple liquid stub tuners, and the optimum liquid levels in the stub tuners can be calculated based on the input impedance. The calculation and adjustment process of the optimum liquid levels are described comprehensively in this article. Finally, impedance matching had been achieved between two shots during EAST experiments. In the near future, a real-time impedance matching system will be developed to prevent large variations of the ICRH antenna impedance and achieve steady-state and long-pulse operation with the ICRH system.

Isolation of microsatellite loci from expressed sequence tag library of Puccinia striiformis f. sp. tritici.

We described twenty polymorphic microsatellite loci derived from the expressed sequence tags of Puccinia striiformis f. sp. tritici, which causes yellow rust disease on wheat. The numbers of alleles range from two to six and eight microsatellite loci show significant similarities to known genes. Observed and expected heterozygosities ranged from 0.12 to 0.78 and from 0.24 to 0.87, respectively.

Thermodynamic pressure of a fluid confined in a random porous medium.

Up to now, none of the previously derived expressions is usable in simulations for the practical calculation of the thermodynamic pressure of a fluid adsorbed in a random porous medium. A bona fide virial expression of this pressure is presented. Contrary to what was believed, we show that this pressure is a measurable quantity and propose an experimental procedure for its measurement.

Quantum tunneling of Bose-Einstein condensates in optical lattices under gravity.

We investigate the quantum tunneling of Bose-Einstein condensates in optical lattices under gravity in the "Wannier-Stark localization" regime and "Landau-Zener tunneling" regime. Our results agree with experimental data [B. P. Anderson et al., Science 282, 1686 (1998); F. S. Cataliotti et al., Science 293, 843 (2001)]. We obtain the total decay rate which is valid over the entire range of temperatures, and show how it reduces to the appropriate results for the classical thermal activation at high temperatures, the thermally assisted tunneling at intermediate temperatures, and the pure quantum tunneling at low temperatures. We design an experimental protocol to observe this new phenomenon in further experiments.

An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct.

The aim of this study was to determine the effects of artificial insemination on the localization of antigen-presenting cells expressing MHC class II mRNA in chicken oviducts. Laying hens (35 weeks old) were inseminated with fresh semen or sham-inseminated with saline daily for 3 days. In situ hybridization was performed to detect chicken MHC class II (B-LB21 major gene) mRNA on frozen sections of oviductal infundibulum, uterovaginal junction and vagina by using digoxigenin-labelled PCR probes. Cells expressing MHC class II were observed mainly in the oviductal mucosal stroma and occasionally in the mucosal epithelium. After 24 h, the population of cells expressing MHC class II in the infundibulum was significantly higher in laying hens inseminated with fresh semen than in the control hens sham-inseminated with saline (P < 0.05). However, there was no significant difference in the population of cells expressing MHC class II in the uterovaginal junction and vagina between the artificially inseminated and control hens. These results indicate that anti-sperm immune responses, including the influx of cells expressing MHC class II and enhanced MHC class II mRNA expression, probably occur in the infundibulum after artificial insemination.

Localization of immunoglobulin G gamma-chain mRNA-expressing cells in the oviduct of laying and diethylstilbestrol-treated immature hens.

The purpose of this study was to determine the synthetic sites of IgG in the chicken oviduct by localizing IgG gamma-chain mRNA (IgGgamma mRNA)-expressing cells and the effects of estrogen on their population. Paraffin sections of oviducal tissues from laying hens (approximately 57 weeks old) and immature hens (approximately 16 weeks old) with or without diethylstilbestrol (DES) treatment were hybridized by digoxigenin-labeled IgG riboprobes or immunostained for IgG gamma-chain (IgG). Immunoreactive IgG was present in some of the mucosal epithelial cells and the plasma cell-like cells in the stromal connective tissue in all segments of the oviduct. In contrast, IgGgamma mRNA expression was observed only in the plasma cell-like cells in the stromal connective tissues, but not in the cells of mucosal epithelium. In laying hens, the lower end of the oviduct, namely the vagina and uterovaginal junction, contained more IgGgamma mRNA-expressing cells than the other segments. Treatment of immature hens with DES for 3 or 6 days increased the population of both IgGgamma mRNA-expressing cells and IgG-containing cells in the oviducal stroma. These results indicate that IgG is locally produced by plasma cell-like cells in the stroma, but not by the cells of the mucosal epithelium, and estrogen may stimulate the infiltration of IgG-producing plasma cell-like cells into the oviducal stroma.

Localization of macrophages in the chicken oviduct: effects of age and gonadal steroids.

The aim of this study was to localize macrophages in the hen oviduct and determine the effects of age and gonadal steroids on their population. Cryostat sections of oviducal tissues from immature hens (60 d of age), virgin young (175 d of age), and old (620 d of age) laying hens, and immature hens (84 d of age) treated with gonadal steroids were immunostained for macrophages. The population of macrophages was analyzed by an image analysis system under a light microscope. Macrophages were observed in the stroma and mucosal epithelium of all oviducal segments of immature and laying hens. The population of macrophages in the oviducal stroma increased with age. Young laying hens had a significantly higher population of macrophages than immature hens in the vagina. Old laying hens had a significantly higher population than immature hens in the infundibulum, magnum, and vagina, and than young laying hens in the magnum. In the immature hens treated with gonadal steroids, the macrophage population increased only in the stroma of shell gland of the progesterone-treated birds. These results indicate that macrophage population in the oviducal stroma increases in association with sexual maturation and aging. Their population in the stroma may be partially affected by progesterone.

Nonperiodic orbit sums in Weyl's expansion for billiards.

Weyl's expansion for the asymptotic mode density of billiards consists of the area, length, curvature, and corner terms. The area term has been associated with the so-called zero-length orbits. Here, closed nonperiodic paths corresponding to the length and corner terms are constructed.

Expression of grass carp growth hormone by baculovirus in silkworm larvae.

A total of five recombinant Bombyx mori nuclear polyhedrosis viruses (BMNPV) carrying the grass carp (Ctenopharyngodon idellus) growth hormone (GH) cDNA were constructed in this study. Two of them were able to express the hormone up to a level of 12 microgram/ml medium when cultured B. mori cells were infected for 4 days. Inoculation of the viruses into silkworm (B. mori) host significantly increased the level of GH achievable. The amount of hormone produced per larva was estimated to be around 1 mg. The recombinant grass carp GH had immunological and biological activities similar to the native hormone. The N-terminal sequence of the recombinant hormone was the same as the native one, indicating that the fish signal peptide was correctly processed by the insect cells. Silkworm powder prepared from larvae infected with the recombinant virus was used as food supplement for fish. Compared with the control, this dietary supplement was effective in increasing the growth rate of juvenile carp.

Effects of age and gonadal steroids on the localization of antigen-presenting cells, and T and B cells in the chicken oviduct.

The effects of age and gonadal steroids on the localization of immunocompetent cells, including antigen-presenting cells that contain the major histocompatibility complex (MHC) class II antigen, and T and B cells in the chicken oviduct were studied. Oviductal tissues were collected from laying and immature hens treated with diethylstilboestrol (an analogue of oestrogen) or progesterone. Cryostat sections of the tissues were immunostained for MHC class II, CD3 (T-cell antigen) and Bu-1 (immature B-cell antigen), and examined under a light microscope and an image analysis system. MHC class II+, CD3+ and Bu-1+ cells were observed in the mucosal epithelium and stromal connective tissue of both the laying and immature hens. MHC class II+ cells in the oviductal stroma appeared in association with oviductal development during sexual maturation and increased with ageing thereafter. The infiltration of CD3+ and Bu-1+ cells into the oviductal tissues increased in young laying hens compared with immature hens and decreased in old laying hens compared with young laying hens. Diethylstilboestrol increased the population of MHC class II+ and CD3+ cells in the stroma of the infundibulum and vagina, but had no significant effect on the population of Bu-1+ cells in the oviduct of immature hens. Progesterone increased the population of CD3+ cells in the stromal tissue of oviductal segments from all hens, and of Bu-1+ cells in the mucosal epithelium of the infundibulum and magnum, but had little effect on the frequency of MHC class II+ cells in the oviduct of immature hens. There were typically more immunocompetent cells in the infundibulum and vagina than in the other oviductal segments in laying hens and immature hens treated with sex steroids. These results suggest that local immunity in the chicken oviduct is enhanced during sexual maturation and possibly decreases during ageing. Gonadal steroids may play a significant role in the regulation of local immunity in the oviduct. The effects of oestrogen and progesterone on the influx of these immunocompetent cells into the oviduct differs among cell types and oviductal segments.

Effects of sexual maturation and gonadal steroids on the localization of IgG-, IgM- and IgA-positive cells in the chicken oviduct.

The effects of sexual maturation and gonadal steroids on the localization of immunoglobulin-positive cells in chicken oviducts were studied. Oviductal tissues were collected from laying hens and chicks treated with stilboestrol (DES, an analogue of oestrogen) or progesterone. Paraffin wax sections of the tissues were immunostained for IgG, IgM and IgA, and the frequency of cells staining positive was examined using an image analysis system. Some of the cells in the mucosal epithelium and plasma cell-like cells in the stroma of the oviduct stained positive for IgG, IgM or IgA. In the mucosal epithelium of laying hens, there was a significantly greater number of IgG-positive (IgG+) cells in the shell gland than in the infundibulum, magnum and isthmus, more IgM+ cells in the magnum than in the infundibulum, and more IgA+ cells in the magnum than in the other segments of the oviduct with the exception of the vagina. The frequency of IgG+ and IgM+ cells in the mucosal epithelium of all oviductal segments and IgA+ cells in the magnum, isthmus and vagina was significantly higher in laying hens than in immature birds. In the subepithelial stroma of laying hens, there was a significantly greater population of IgG+ cells in the infundibulum and vagina than in the magnum and isthmus, more IgM+ cells in the infundibulum than in the magnum, and more IgA+ cells in the uterovaginal junction and vagina than in the magnum and isthmus. The frequency of IgG+, IgM+ and IgA+ cells in the subepithelium of infundibulum, uterovaginal junction and vagina was significantly greater in laying hens than in immature birds. The number of IgM+ cells in all oviductal segments and of IgA+ cells in the magnum of the mucosal epithelium of the chicks treated with DES increased significantly compared with those of control chicks. In addition, the number of IgG+ cells in the shell gland and vagina and of IgM+ cells in the vagina of the stroma of DES-treated birds were increased. Treatment of immature birds with progesterone had no effect on the localization of Ig+ cells in the oviduct except for a decrease in the number of IgM+ cells in the shell gland. These results suggest that the local immunity in the oviduct develops during sexual maturation, possibly under the control of oestrogen.

Rapid arrest of seizures with an inhalation aerosol containing diazepam.

Diazepam (DZP) and a mixture of Chinese herbs customarily used to treat epilepsy were prepared as an aerosol under the trade name Aerosolum Diaiepami Compositae or Flvalscop (FVS). FVS was studied in a single-blind trial in 101 patients with seizures preceded by an aura and in 19 without an aura to whom was administered by another person. FVS or a control preparation was administered. In 16-22 s, (average 18.5 s), the aura was interrupted and no seizure ensued in 90% of the cases treated with FVS and in 26% of cases treated with the control preparation. Of the 120 patients, 8 had elementary partial seizures with Jacksonian march, 18 had complex partial seizures (CPS), 7 had simple partial seizures with autonomic symptoms, and 87 had secondarily generalized tonic-clonic seizures. Eleven patients have now received FVS for 2 years (400 ml each). Forty patients for 1 year (150-200 ml each); none of these patients have shown any side effects or abnormal laboratory findings. An aerosol-administered drug may be a valuable adjunct to the antiepileptic drug (AED) arsenal and merits more extensive evaluation.

A system control framework for the self-fertilization and selection process of breeding.

The self-fertilization and selection process is the main method for speeding up the purifying course of a hybrid population in breeding. This is a complex combinatorial random process with large time delay. To raise the control effectiveness and efficiency of the process, we try, in this paper, to construct a mathematical model of the process by means of effective factors. Then, a control framework for the process is presented which can be used as a guide by breeders for helping them in selecting appropriate control actions.

Gene (effective factor) model and its estimation.

In order to analyze quantitative characters effectively, a gene (effective factor) model is established, which can describe the phenotypic distribution of a quantitative character in the segregating population quite well because it has not only involved the additive effect of genes, but has also considered the dominance deviation and epistatic deviation. In this paper, we also present a method to estimate the parameters of the model. Based on the results of some illustration, the proposed model is considered to be superior to Castle-Wright's formula, and it may provide more useful information to breeders.

The effect of intrauterine devices (stainless steel ring, copper-T220 and levonorgestrel) on the DNA content in isolated human endometrial cells.

The effect of three different intrauterine devices on the DNA content in human endometrial cells has been studied in 29 women before and after the insertion of an IUD; a stainless steel ring (9 subjects), Copper-T220 (11 subjects) and a medicated IUD releasing levonorgestrel at a rate of 2 micrograms/day (9 subjects). The material was grouped into "bleeders" (having more than 8 days of bleeding and spotting per month) and "non-bleeders" (having less than 8 days of bleeding and spotting per month). The DNA content was assessed on isolated endometrial cells smeared from thin biopsies and the amount of DNA per cell nucleus was measured by means of a Feulgen microspectrophotometric method. No significant difference was found between the "bleeders" and the "non-bleeders" as to the DNA content. Nevertheless, when the total number of subjects were pooled together, a significant increase in the DNA content was found in the post-insertion specimens in the groups of women using the SS and the T-Cu IUDs. Furthermore, a significant decrease in the DNA content was also found when the specimens obtained after the insertion of the LNG IUD were compared with those obtained after 24 months' use of the stainless steel ring and the Copper-T220 device (p less than 0.01 and p less than 0.002, respectively). It is concluded that the insertion of an inert IUD or an T-Cu IUD, induces significant alterations in the DNA content of the human endometrium adjacent to the device, whereas the use of the LNG device does not significantly modify the DNA synthesis when used for 3-10 months. No correlation was found between the DNA content per cell and the number of days of bleeding and spotting.