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1.
Infect Dis Model ; 9(2): 618-633, 2024 Jun.
Article in English | MEDLINE | ID: mdl-38645696

ABSTRACT

The rapid acceleration of global warming has led to an increased burden of high temperature-related diseases (HTDs), highlighting the need for advanced evidence-based management strategies. We have developed a conceptual framework aimed at alleviating the global burden of HTDs, grounded in the One Health concept. This framework refines the impact pathway and establishes systematic data-driven models to inform the adoption of evidence-based decision-making, tailored to distinct contexts. We collected extensive national-level data from authoritative public databases for the years 2010-2019. The burdens of five categories of disease causes - cardiovascular diseases, infectious respiratory diseases, injuries, metabolic diseases, and non-infectious respiratory diseases - were designated as intermediate outcome variables. The cumulative burden of these five categories, referred to as the total HTD burden, was the final outcome variable. We evaluated the predictive performance of eight models and subsequently introduced twelve intervention measures, allowing us to explore optimal decision-making strategies and assess their corresponding contributions. Our model selection results demonstrated the superior performance of the Graph Neural Network (GNN) model across various metrics. Utilizing simulations driven by the GNN model, we identified a set of optimal intervention strategies for reducing disease burden, specifically tailored to the seven major regions: East Asia and Pacific, Europe and Central Asia, Latin America and the Caribbean, Middle East and North Africa, North America, South Asia, and Sub-Saharan Africa. Sectoral mitigation and adaptation measures, acting upon our categories of Infrastructure & Community, Ecosystem Resilience, and Health System Capacity, exhibited particularly strong performance for various regions and diseases. Seven out of twelve interventions were included in the optimal intervention package for each region, including raising low-carbon energy use, increasing energy intensity, improving livestock feed, expanding basic health care delivery coverage, enhancing health financing, addressing air pollution, and improving road infrastructure. The outcome of this study is a global decision-making tool, offering a systematic methodology for policymakers to develop targeted intervention strategies to address the increasingly severe challenge of HTDs in the context of global warming.

2.
Fa Yi Xue Za Zhi ; 31(5): 373-6, 380, 2015 Oct.
Article in Chinese | MEDLINE | ID: mdl-26821481

ABSTRACT

OBJECTIVE: To establish a multiplex STR genotyping method for autosomal STR and Y-STR loci in forensic biological practice. METHODS: Widely used autosomal STR loci and Y-STR loci were selected. A set of PCR primers was designed, and a 5-dye fluorescent labeled STR multiplex PCR reagent kit was developed. RESULTS: A kit was developed which can simultaneously detect 15 autosomal STR loci, 10 Y-STR loci, and an Amelogenin. CONCLUSION: The 15 autosomal STR plus 10 Y-STR kit in combination with capillary electrophoresis method was used to STR genotyping with accurate and reliable results. The new one-step testing kit can potentially be widely used in forensic cases and DNA databank in the future.


Subject(s)
Genotyping Techniques/instrumentation , Microsatellite Repeats , Multiplex Polymerase Chain Reaction , Amelogenin , Chromosomes, Human, Y/genetics , DNA Primers , Databases, Nucleic Acid , Forensic Genetics/methods , Genotype , Humans , Indicators and Reagents
3.
Fa Yi Xue Za Zhi ; 28(6): 448-50, 2012 Dec.
Article in Chinese | MEDLINE | ID: mdl-23484329

ABSTRACT

OBJECTIVE: To explore the application value of Expressmarker 22 STR loci direct PCR amplification kit. METHODS: One thousand nine hundred and forty-eight samples (including samples spotted on FTA cards, filter papers and case samples) were tested using Expressmarker 22 STR loci direct PCR amplification kit. At the same time, all were tested using Sinofiler kit, Identifiler kit and PowerPlex 16 kit respectively for comparison. The genotypes were compared at the same STR loci among these four kits to test the sensitivity and accuracy of Expressmarker 22 STR loci direct PCR amplification kit. RESULTS: 97.79% samples were successfully typed using Expressmarker 22 STR loci direct PCR amplification kit. The genotype profiles of the same samples using Expressmarker 22 STR loci direct PCR amplification kit were consistent with Sinofiler kit, Identifiler kit and PowerPlex 16 kit at the same STR loci. CONCLUSION: Expressmarker 22 STR loci direct PCR amplification kit can provide huge information and accurate results


Subject(s)
DNA Fingerprinting/methods , DNA/analysis , Microsatellite Repeats , Polymerase Chain Reaction/methods , Alleles , DNA/genetics , DNA Primers , Forensic Genetics/methods , Genetic Loci/genetics , Genotype , Humans , Polymerase Chain Reaction/instrumentation
4.
Fa Yi Xue Za Zhi ; 27(6): 444-6, 2011 Dec.
Article in Chinese | MEDLINE | ID: mdl-22393596

ABSTRACT

OBJECTIVE: To establish a rapid STR genotyping method for individual identification. METHODS: Two hundred blood samples from FTA were collected. Equal amount of blood were collected by puncher and analyzed using two methods (6+1 STR kit in combination with EX-Q20 electrophoresis and Sinofiler kit in combination with POP4 electrophoresis). Consuming time and results of two methods were compared. RESULTS: 6+1 STR kit in combination with EX-Q20 electrophoresis method can obtain all genotyping results and be shorter time. CONCLUSION: 6+1 STR kit in combination with EX-Q20 electrophoresis method is used to STR genotyping with accurate, reliable results and this new method is potential value in mass personnel investigation and comparison in major criminal cases. It also can raise the work efficiency.


Subject(s)
Blood Stains , DNA/analysis , Electrophoresis, Capillary/methods , Genotyping Techniques/methods , Reagent Kits, Diagnostic , Tandem Repeat Sequences , Alleles , DNA/genetics , DNA Fingerprinting/methods , DNA Primers , Forensic Medicine/methods , Genotype , Humans , Polymerase Chain Reaction/methods , Reproducibility of Results
5.
Zhongguo Zhen Jiu ; 25(2): 85-6, 2005 Feb.
Article in Chinese | MEDLINE | ID: mdl-16312885

ABSTRACT

OBJECTIVE: To search for a method for increasing therapeutic effect on vitiligo. METHODS: One hundred and sixteen cases were randomly divided into a treatment group and a control group, 58 cases in each group. The treatment group were treated with electro-plum-blossom needle therapy plus catgut implantation at local acupoints under TDP radiation, and the control group with external application of sicorten. Their short-term and long-term therapeutic effects were observed. RESULTS: The short-term total effective rate was 98.3% in the treatment group and 74.1% in the control group with a significant difference between the two groups (P < 0.05). CONCLUSION: Electro-plum-blossom needle therapy plus catgut implantation at acupoints has a better therapeutic effect on vitiligo with no adverse effect.


Subject(s)
Acupuncture Points , Catgut , Acupuncture Therapy , Flowers , Humans , Needles , Prunus domestica , Vitiligo
6.
Ai Zheng ; 23(8): 885-9, 2004 Aug.
Article in Chinese | MEDLINE | ID: mdl-15301708

ABSTRACT

BACKGROUND & OBJECTIVE: Recent studies have shown that overexpression of Fas associated phosphatase-1 (FAP-1) can been detected in human ovarian cancer cell line SKOV3, suggesting that this overexpression may play an important role in the tumorigenesis and drug resistance of ovarian cancer. This study was designed to explore the effects of fas associated phosphatase-1 antisense oligonucleotide (FAP-1 ASODN) combined with carboplatin on the apoptosis of ovarian cancer cell SKOV3. METHODS: Antisense oligonucleotide technique was used to transfect FAP-1 ASODN into SKOV3 cells. The expression levels of FAP-1 mRNA of SKOV3 cells with or without FAP-1 ASODN transfection were determined by reverse transcription- polymerase chain reaction (RT-PCR). The cell cycle and apoptotic rate were analyzed by flow cytometry (FCM). The growth and proliferation of SKOV3 cells were observed by MTT assay. RESULTS: After transfected FAP-1 ASODN for 24 hours, the expression of FAP-1 mRNA in SKOV3 cells was obviously reduced compared with those of the control and FAP-1 SODN transfection groups. When induced apoptosis with 40 microg/ml carboplatin for 24-72 hours, FCM results showed the apoptotic rate of "carboplatin+FAP-1 ASODN" group was higher than those of "carboplatin" group and "ASODN" group (P< 0.05), and the cell cycle was blocked in G1 phase. MTT results showed the cell inhibitory rate of "carboplatin+FAP-1 ASODN" group was 1.5-2 times those of "carboplatin" group and "ASODN" group (P< 0.05); but no significant difference was found between "carboplatin" group and "carboplatin+FAP-1 SODN" group (P >0.05). CONCLUSION: FAP-1 ASODN transfection can suppress the expression of FAP-1 gene in SKOV3 cells and enhance the cell sensitivity to apoptosis induced by carboplatin, which implies that FAP-1 ASODN may reverse the drug resistance in ovarian cancer.


Subject(s)
Apoptosis/drug effects , Carboplatin/pharmacology , Carrier Proteins/biosynthesis , Oligonucleotides, Antisense/pharmacology , Ovarian Neoplasms/pathology , Protein Tyrosine Phosphatases/biosynthesis , Adenocarcinoma/metabolism , Adenocarcinoma/pathology , Antineoplastic Agents/pharmacology , Carrier Proteins/genetics , Cell Cycle/drug effects , Cell Line, Tumor , Cell Proliferation/drug effects , Female , Humans , Oligonucleotides, Antisense/genetics , Ovarian Neoplasms/metabolism , Protein Phosphatase 1 , Protein Tyrosine Phosphatase, Non-Receptor Type 13 , Protein Tyrosine Phosphatases/genetics , Transfection
7.
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi ; 19(2): 140-1, 2003 Mar.
Article in Chinese | MEDLINE | ID: mdl-15151750

ABSTRACT

AIM: To clone the PID domain of human DOC-2 (nDOC-2) and express it in E.coli DH5alpha. METHODS: The cDNA fragment encoding the PID domain of nDOC-2 was amplified by RT-PCR from normal human ovarian tissue and cloned to pUC19. The DNA fragment from the pUC19-nDOC-2 digested with BamH I and EcoR I was ligated to the BamH I/EcoR I digested prokaryotic expression vector pGEX-4T-1.The expression of fusion protein was induced with IPTG and the expressed product was identified by SDS-PAGE. RESULTS: (1)The sequencing and endonucleases digestion analysis showed that the fragment of nDOC-2 gene was insert into vectors pUC19 and pGEX-4T-1;(2)SDS-PAGE showed the nDOC-2 gene had been expressed in E.coli DH5alpha. CONCLUSION: The PID domain of nDOC-2 was expressed successfully in prokaryote, which makes preparation for further researching the function of DOC-2 and preparing antibodies to DOC-2 protein.


Subject(s)
Gene Expression , Plasmids , Cloning, Molecular , Electrophoresis, Polyacrylamide Gel , Escherichia coli/genetics , Genetic Vectors , Humans
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