ABSTRACT
AIM To establish an HPLC method for the simultaneous content determination of six constituents in Jiaotai Pills (Coptidis Rhizoma and Cinnamomi Cortex).METHODS The analysis of 30％ methanol of this drug was performed on a 30 ℃ Agilent ZORBAX SB-C1s column (4.6 mm ×250 mm,5 μm),with the mobile phase comprising of acetonitrile-KH2PO4flowing at 0.8 mL/min in a gradient elution manner,and the detection wavelength was set at 276 nm.RESULTS Epiberberine,jatrorrhizine hydrochloride,coptisine hydrochloride,palmatine chloride,berberine hydrochloride and cinnamaldehyde showed good linear relationships within the ranges of 0.64-41.24 μg/mL (R2 =0.999 9),0.65-43.76 μg/mL (R2 =1.000 0),0.82-52.65 μg/mL (R2 =0.999 9),0.79-50.70 μg/mL (R2 =0.999 9),3.08-197.20 μg/mL (R2=0.999 8) and 0.65-41.65 μg/mL (R2 =0.999 9),whose average recoveries were 98.06％,102.76％,99.27％,99.75％,96.74％ and 101.33％ with the RSDs of 0.56％,0.54％,0.39％,0.55％,0.48％ and 2.14％,respectively.CONCLUSION This accurate,sensitive,stable and reproducible method can be used for the quality control of Jiaotai Pills.
ABSTRACT
PURPOSE: The aim of this study is to investigate the relationship between ERK1, ERK2 gene expression and tumor behavior such as invasion, metastasis in tongue squamous cell carcinoma (TSCC). METHODS: The expressions of ERK1, ERK2 at gene level were examined in 45 cases of TSCC by reverse transcriptase polymerase chain reaction(RT-PCR). Student's t test was used for data analysis using SPSS 12.0 software package. RESULTS: No correlation between the expression intensity of ERK gene and tumor pathological degree of TSCC.A significant relationship was observed between the expression intensity of ERK gene and T stage of TSCC. A significant relationship was observed between the expression intensity of ERK gene and lymphnode metastasis of TSCC. CONCLUSIONS: Activation of ERK gene may play a strong role in cervical lymph node metastasis of TSCC. Intensive expression of ERK may be an important presage marker in TSCC patient. Supported by Key Science and Technology Research Project of Liaoning Province (Grant No.2005225013-2).
