The presence of copper ions alters tetracycline removal pathway in aerobic granular sludge: Performance and mechanism.

This study investigated the removal characteristics of tetracycline (TC) in the presence of copper ions (Cu2+) in aerobic granular sludge by analyzing the TC removal pathway, composition and functional group changes of extracellular polymeric substances (EPS), and microbial community structure. The TC removal pathway changed from cell biosorption to EPS biosorption, and the microbial degradation rate of TC was reduced by 21.37% in the presence of Cu2+. Cu2+ and TC induced enrichment of denitrifying bacteria and EPS-producing bacteria by regulating the expression of signaling molecules and amino acid synthesis genes to increase the content of EPS and -NH2 groups in EPS. Although Cu2+ reduced the content of acidic hydroxyl functional groups (AHFG) in EPS, an increase in TC concentration stimulated the secretion of more AHFG and -NH2 groups in EPS. The long-term presence of TC presence of the relative abundances of Thauera, Flavobacterium and Rhodobacter and improved the removal efficiency.

Iron-loaded sludge biochar alleviates the inhibitory effect of tetracycline on anammox bacteria: Performance and mechanism.

The anaerobic ammonia oxidation (anammox) process is sensitive to environmental pollutants, such as antibiotics. In this study, the harmful effect of tetracycline (TC) on the performance of an anammox reactor and the mitigation of TC inhibition by iron-loaded sludge biochar (Fe-BC) were studied by analyzing extracellular polymeric substances (EPS), microbial community structure and functional genes. The total inorganic nitrogen (TIN) removal rate of the TC reactor was reduced by 5.86% compared to that of the control group, while that of the TC + Fe-BC reactor improved by 10.19% compared to that of the TC reactor. Adding Fe-BC increased the activity of anammox sludge by promoting the secretion of EPS (including protein, humic acids and c-Cyts). The results of the enzymolysis experiment showed that protein can improve the activity of anammox sludge, while the ability of polysaccharide to improve the activity of anammox was related to the treated enzymes. In addition, Fe-BC alleviated the inhibitory effect of TC by mediating the anammox electron transfer process. Furthermore, Fe-BC increased the absolute abundance of hdh and hzsB by 2.77 and 1.18 times compared to the TC reactor and improved the relative abundance of Candidatus Brocadia in the absence of TC. The addition of Fe-BC is an effective way to alleviate the inhibitory effect of TC on the anammox process.

The coexistence of copper ions and TC affected the binding ability and the reaction order between extracellular polymeric substances of aerobic granular sludge and exogenous substances.

As a barrier against external toxic effects, extracellular polymeric substances (EPSs) directly affect the toxicity and removal efficiency of exogenous substances. The reaction of EPSs with exogenous substances has been taken into consideration. The contents of EPSs in sludge cultivated by different influent water vary greatly, which leads to great differences in the binding ability and reaction sequence between EPSs and exogenous substances. However, the results in this respect are very limited. In this study, the binding characteristics between exogenous tetracycline (TC)/copper ions (Cu2+) and EPSs from aerobic granular sludge cultured under single and coexisting TC/Cu2+ were assessed by three-dimensional fluorescence-parallel factor analysis. The pollutants in the influent water could directionally induce microorganisms to secrete more EPSs, while fluorescence substances in EPSs could combine with the exogenous substances to lessen their effects. In the presence of coexisting TC and Cu2+ in the influent water, the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu2+ weakened, and humic substances in EPSs were more susceptible than protein substances to binding with exogenous substances. However, the reaction order between EPSs components and exogenous TC or Cu2+ was opposite, and the ability of fluorescence substances in EPSs to combine with exogenous TC or Cu2+ was enhanced under individual TC or Cu2+ existing in the influent water. This study provided new insights into the interaction between EPSs and exogenous substances.

Step feed mode synergistic mixed carbon source to improve sequencing batch reactor simultaneous nitrification and denitrification efficiency of domestic wastewater treatment.

The limited efficiency of nitrogen removal has traditionally hindered wide application of simultaneous nitrification and denitrification (SND) technology. Here, the nitrogen removal characteristics of a sequencing batch reactor were studied by adopting a strategy of a step-feeding mode, synergistic regional oxygen limitation, and a mixed carbon source. The changes of the microbial population succession and nitrogen metabolism functional genes were analyzed. This strategy provided a favorable level of dissolved oxygen and continuous carbon sources for driving the denitrification process. The total nitrogen removal efficiency and SND rate reached 92.60% and 96.49%, respectively, by regulating the ratio of sodium acetate to starch in the step feed to 5:1. This procedure increased the relative abundance of denitrifying functional genes and induced the growth of a variety of traditional denitrifying bacteria and aerobic denitrifying bacteria participating in the process of nitrogen removal. Overall, this work offers a new strategy for achieving efficient SND.

Performance and mechanism of tetracycline removal by the aerobic nitrate-reducing strain Pseudomonas sp. XS-18 with auto-aggregation.

The coexistence of multiple pollutants has become a distinctive feature of water pollution. However, there are a few strains that can remove nitrate and tetracycline (TC). Here, the efficiency of strain XS-18 in removing nitrate and TC was analyzed, and the mechanism of tolerance and removal of TC was investigated by infrared spectroscopy, three-dimensional fluorescence spectroscopy, and genome analysis. XS-18 could efficiently remove TC (0.40 mg·L-1·h-1) at pH 7.0-11.0 with auto-aggregation. TC was removed via extracellular polymeric substance (EPS) (55.90%) and cell surface (44.10%) adsorption. TC (10 mg/L) could stimulate XS-18 to secrete more polysaccharides and hydrophobic proteins to improve its auto-aggregation ability. The findings also confirmed that TC resistance genes were present. Furthermore, the bacterial flagellum, signal transduction of the chemotactic system and regulatory genes were shown to be related to the auto-aggregation of the strain. XS-18 has potential applications in the treatment of wastewater containing nitrate and TC.

Nitrate removal by alkali-resistant Pseudomonas sp. XS-18 under aerobic conditions: Performance and mechanism.

To improve poor nitrate removal by microorganisms under strong alkaline conditions, a new type of aerobic nitrification-reducing bacteria was isolated in this study. Using nitrogen balance and genome information, the capacity of Pseudomonas XS-18 to remove nitrate and the mechanism of alkali tolerance were analyzed. At pH 11.0, XS-18 could remove 12.17 mg N/(L·h) nitrate. At C/N ratios of 13.0 and 25 °C, nitrite and ammonia nitrogen were barely enriched. XS-18 could reduce nitrate through dissimilation and assimilation, and 21.74% and 77.39% of nitrate was converted into cellular components and organic nitrogen, respectively. Meanwhile, functional genes (nirBD, nasAB, gdhA, glnA, and gltBD) associated with nitrogen metabolism were determined. In addition, Na+/H+ antiporters (MnhACDEFG, PhaACDEFG, NhaCD and TrkAH) and a cell surface protein (SlpA) from the XS-18 genome, as well as compatible solutes that help stabilize intracellular pH, were also characterized. XS-18 possessed significant potential in alkaline wastewater treatment.

Tetracycline removal in granulation: Influence of extracellular polymers substances, structure, and metabolic function of microbial community.

Tetracycline is a potentially hazardous residual antibiotic detected in various sewages. High concentration (mg/L) of tetracycline is found in pharmaceutical/hospital wastewater and wastewater derived from livestock and poultry. So far, only antibiotics in µg/L level have been reported in granulation of aerobic sludge during wastewater treatment, but its effects in high concentration are rarely reported. In this study, the influence of tetracycline in high concentration (∼2 mg/L) on the formation of granular sludge, structure, and metabolic function of the microbial community during the granulation of aerobic sludge was investigated to improve the understanding of the aerobic granular sludge formation under high-level of tetracycline. The role of extracellular polymers substances (EPSs) derived from granular sludge in the granulation and tetracycline removal process was also investigated, showing that tetracycline improved the relative hydrophobicity, flocculability and protein/polysaccharide ratio of EPSs, accelerating the granulation of sludge. Succession of microbial communities occurred during the domestication of functional bacteria present in the sludge and was accompanied with regulation of metabolic function. The addition of tetracycline lead to an increase of tetracycline-degrading bacteria or antibiotic resistance genus. Those findings provide new perspectives of the influence of tetracycline on aerobic sludge granulation and the removal mechanism of tetracycline.

Short UV-B Exposure Stimulated Enzymatic and Nonenzymatic Antioxidants and Reduced Oxidative Stress of Cold-Stored Mangoes.

The effects of UV-B irradiation on reactive oxygen species (ROS) levels, antioxidant compound contents, antioxidative enzyme activities, and oxidative damage of cold-stored mangoes were examined. Superoxide anion production rate, hydrogen peroxide concentration, ion leakage level and malondialdehyde content of the cold-stored fruit preradiated with 5 KJ m(-2) UV-B for 4 h were significantly decreased as compared with control fruit. The activities of ROS generating enzymes remained unchanged in UV-B-irradiated mangoes as compared to the control, but superoxide dismutase and catalase activities, ascorbate and polyphenol contents and antioxidant capacities of the cold-stored mangoes were significantly enhanced by UV-B. The UV-B-enhanced antioxidant compounds and antioxidative enzymes were highly correlated with the reduced-ROS levels in UV-B-irradiated mangoes. The data indicated that a short UV-B exposure reduced oxidative stress and alleviated oxidative damage of the cold-stored mangoes by triggering both enzymatic and nonenzymatic antioxidant systems although ROS generation in the fruit was not affected.