Dietary Macronutrient Intake and Cardiovascular Disease Risk and Mortality: A Systematic Review and Dose-Response Meta-Analysis of Prospective Cohort Studies.

Many epidemiological studies have evaluated the intake of macronutrients and the risk of mortality and cardiovascular disease (CVD). However, current evidence is conflicting and warrants further investigation. Therefore, we carried out an umbrella review to examine and quantify the potential dose-response association of dietary macronutrient intake with CVD morbidity and mortality. Prospective cohort studies from PubMed, Embase, and CENTRAL were reviewed, which reported associations of macronutrients (protein, fat, and carbohydrate) with all-cause, CVD, cancer mortality, or CVD events. Multivariable relative risks (RR) were pooled, and heterogeneity was assessed. The results of 124 prospective cohort studies were included in the systematic review and 101 in the meta-analysis. During the follow-up period from 2.2 to 30 years, 506,086 deaths and 79,585 CVD events occurred among 5,107,821 participants. High total protein intake was associated with low CVD morbidity (RR 0.88, 95% confidence interval 0.82-0.94), while high total carbohydrate intake was associated with high CVD morbidity (1.08, 1.02-1.13). For fats, a high intake of total fat was associated with a decreased all-cause mortality risk (0.92, 0.85-0.99). Saturated fatty acid intake was only associated with cancer mortality (1.10, 1.06-1.14); Both monounsaturated fatty acid (MUFA) and polyunsaturated fatty acids (PUFA) intake was associated with all-cause mortality (MUFA: 0.92, 0.86-0.98; PUFA: 0.91, 0.86-0.96). This meta-analysis supports that protein intake is associated with a decreased risk of CVD morbidity, while carbohydrate intake is associated with an increased risk of CVD morbidity. High total fat intake is associated with a low risk of all-cause mortality, and this effect was different in an analysis stratified by the type of fat.

Dual-Modal Biosensor for Staphylococcus aureus Detection Based on a Porphyrin-Based Porous Organic Polymer FePor-TPA with Excellent Peroxidase-like, Catalase-like, and Photoelectrochemical Properties.

Bacterial infections seriously harm human health and cause many severe diseases, which triggered urgent demands to exploit specific and sensitive biosensor strategies for Staphylococcus aureus detection. Here, a colorimetric and photoelectrochemical dual-mode biosensor for S. aureus assay based on FePor-TPA was constructed. 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were synthesized by in situ growth on ITO and a solvothermal condition, respectively, both of which exhibited excellent peroxidase-like and catalase-like activity, originating from their metalloporphyrin linkers. Benefiting from the in situ growth on ITO electrodes, the 2D FePor-TPA thin film also possessed a more ordered stacking mode and in turn exhibited good electrical conductivity, stable initial photocurrent, and high sensitivity to O2. As for bulk FePor-TPA, its porous structure and high specific surface area make it a possible scaffold to load an amount of AuNPs, the rabbit anti-Staphylococcus aureus Rosenbach tropina antibody (Ab2), and GOx for constructing the signal probe (GOx/Ab2@Au@FePor-TPA) and realizing catalytic amplification. With these satisfactory features in mind, the 2D FePor-TPA thin film and its bulk powder (FePor-TPA) were utilized to construct a dual and signal-on bioplatform for sensitively and selectively detecting S. aureus, which, as far as we know, has not been reported.

Advances in the Study of Bioactive Nanoparticles for the Treatment of HCC and Its Postoperative Residual Cancer.

Primary hepatocellular carcinoma (HCC, hepatocellular carcinoma) is the third leading cause of tumor death in the world and the second leading cause in China. The high recurrence rate at 5 years after surgery also seriously affects the long-term survival of HCC patients. For reasons such as poor liver function, large tumors, or vascular invasion, only relatively limited palliative treatment is available. Therefore, effective diagnostic and therapeutic strategies are needed to improve the complex microenvironment and block the mechanism of tumor development in order to treat the tumor and prevent recurrence. A variety of bioactive nanoparticles have been shown to have therapeutic effects on hepatocellular carcinoma and have the advantages of improving drug solubility, reducing drug side effects, preventing degradation in the blood, increasing drug exposure time, and reducing drug resistance. The development of bioactive nanoparticles is expected to complete the current clinical therapeutic approach. In this review, we discuss the therapeutic advances of different nanoparticles for hepatocellular carcinoma and discuss their potential for postoperative applications with respect to possible mechanisms of hepatocellular carcinoma recurrence. We further discuss the limitations regarding the application of NPs and the safety of NPs.

Physical Frailty, Genetic Predisposition, and Incident Parkinson Disease.

Importance: Cross-sectional evidence implicates high prevalent frailty in patients with Parkinson disease (PD), whereas the longitudinal association remains unknown. Objectives: To examine the longitudinal association of the frailty phenotype with the development of PD and to explore the modification role of genetic risk of PD in such an association. Design, Setting, and Participants: This prospective cohort study launched in 2006 to 2010 with a follow-up of 12 years. Data were analyzed from March 2022 to December 2022. The UK Biobank recruited over 500 000 middle-aged and older adults from 22 assessment centers across the United Kingdom. Participants who were younger than 40 years (n = 101), diagnosed with dementia or PD at baseline, and developed dementia, PD, or died within 2 years from baseline were excluded (n = 4050). Participants who had no genetic data or mismatch between genetic sex and reported gender (n = 15 350), were not of self-reported British White descent (n = 27 850), and had no data for frailty assessment (n = 100 450) or any covariates were also excluded (n = 39 706). The final analysis included 314 998 participants. Exposures: The physical frailty was assessed by the Fried criteria's frailty phenotype through 5 domains, ie, weight loss, exhaustion, low physical activity, slow walking speed, and low grip strength. The polygenic risk score (PRS) for PD comprised 44 single-nucleotide variants. Main Outcomes and Measures: New-onset PD was identified through the hospital admission electronic health records and death register. Results: Among 314 998 participants (mean age, 56.1 years; 49.1% male), 1916 new-onset PD cases were documented. Compared with nonfrailty, the hazard ratio (HR) of incident PD in prefrailty and frailty was 1.26 (95% CI, 1.15-1.39) and 1.87 (95% CI, 1.53-2.28), respectively, and the absolute rate difference per 100 000 person-years was 1.6 (95% CI, 1.0-2.3) for prefrailty and 5.1 (95% CI, 2.9-7.3) for frailty. Exhaustion (HR, 1.41; 95% CI, 1.22-1.62), slow gait speed (HR, 1.32; 95% CI, 1.13-1.54), low grip strength (HR, 1.27; 95% CI, 1.13-1.43), and low physical activity (HR, 1.12; 95% CI, 1.00-1.25) were associated with incident PD. A significant interaction between frailty and PRS on PD was found and the highest hazard was observed in participants with frailty and high genetic risk. Conclusions and Relevance: Physical prefrailty and frailty were associated with incident PD independent of sociodemographic factors, lifestyles, multiple morbidities, and genetic background. These findings may have implications for the assessment and management of frailty for PD prevention.

Experimental Study on the Ethanol-Assisted Catalytic Steam Reforming of Endothermic Hydrocarbon Fuel.

Thermal protection is a critical problem in the development of hypersonic aircraft. To enhance the thermal protection capability of hydrocarbon fuel, the ethanol-assisted catalytic steam reforming of endothermic hydrocarbon fuel was proposed. The result shows that the total heat sink can be significantly improved by the endothermic reactions of ethanol. A higher water/ethanol ratio can promote the steam reforming of ethanol and further increase the chemical heat sink. The addition of 10 wt % ethanol at 30 wt % water content can improve the total heat sink by 8-17% at 300-550 °C, which is caused by the heat absorption by phase transition and chemical reactions of ethanol. The reaction region of thermal cracking moves backward, resulting in the suppression of thermal cracking. Meanwhile, the addition of ethanol can inhibit the coke deposition and increase the working temperature upper limit of the active thermal protection.

Secondary Flow Effect on Supercritical Kerosene Oxidation Deposition and Heat Transfer in a Coiled Tube.

Deposition in fuel cooling systems remains a challenge to the development of active cooling technologies for air-breathing engines. We experimentally and numerically investigated the influence of the secondary flow and heat-transfer characteristics of supercritical kerosene in a coiled tube on oxidation deposition. A coiled heated tube reactor (3000 mm long, 23 cycles) under constant heat flux and flow rate was applied to simulate the conditions of the fuel side in the heat exchanger of an aero-engine cooling system. The coupling characteristics of coking distribution with the development of secondary flow were studied along the whole pipe. The dynamic pressure, temperature, and velocity were analyzed in two specific circular cross sections located in the bend of the tube. The secondary flows induced in the coiled tube greatly enhance the heat transfer and slightly decrease the deposition rate, resulting in linear wall temperature profiles and a uniform coking distribution along the tube compared to the long straight tube. There is no obvious heat-transfer enhancement or deterioration in the whole coiled tube. The modified heat-transfer correlation of the supercritical RP-3 in the coiled tube was fitted at different flow rates and heat fluxes with an error of ±10%.

Sensitive electrochemical biosensor for Uracil-DNA glycosylase detection based on self-linkable hollow Mn/Ni layered doubled hydroxides as oxidase-like nanozyme for cascade signal amplification.

Nanozymes have been widely used in biosensors instead of natural enzymes because of low cost, high stability and ease of storage. However, few works use oxidase-like nanozymes to fabricate electrochemical biosensors. Herein, we proposed a sensitive electrochemical biosensor to detect uracil-DNA glycosylase (UDG) based on the hollow Mn/Ni layered doubled hydroxides (h-Mn/Ni LDHs) as oxidase-like nanozyme. Briefly, the h-Mn/Ni LDHs, which was prepared by a facile hydrothermal method, exhibited excellent oxidase-like activity because the hollow structure provided rich active sites and high specific surface area. Then, the signal probes were constructed by assembling the hairpin DNA (hDNA), single DNA1 and DNA2 on the h-Mn/Ni LDHs, respectively. In the presence of UDG, the uracil bases in the stem of hDNA were specifically excised, generating apyrimidinic (AP) sites and inducing the unwinding of the hDNA. Afterwards, the h-Mn/Ni LDHs@Au-hDNA/DNA1 was connected on the electrode via hybridization between unwinded hDNA and capture DNA (cDNA). Subsequently, the self-linking process allowed the retention of numerous h-Mn/Ni LDHs through simple DNA hybridization to amplify the signal of o-phenylenediamine (o-PD). Unlike many peroxidase-like nanozyme-based electrochemical biosensors, there is no need to add H2O2 during the experimental process, which effectively reduced the background signal as well as improved the stability of the biosensor. As expected, the biosensor exhibited excellent performance with a wide linear range and a low detection limit. This work highlights an appealing opportunity to develop a no H2O2 platform based on h-Mn/Ni LDHs for future application in biological analysis and clinical diagnosis.

Mechanism of Stability Enhancement for Adiponitrile High Voltage Electrolyte System Referring to Addition of Fluoroethylene Carbonate.

In order to improve the stability of high voltage electrolyte for 5 V-level LiNi0.5Mn1.5O4 cathode material, adiponitrile (ADN) with high oxidation stability was selected as the main solvent, meanwhile, 2% fluoroethylene carbonate (FEC) as the additive with good film forming effect was also used. And then, the effect of 2 mol L-1 LiBF4-GBL/ADN+2% FEC on the electrochemical performance of LiNi0.5Mn1.5O4 was explored at room temperature. The electrolyte system containing FEC can improve the cycle stability of the battery. At 1 C rate, the cycle capacity retention rate can reach 83% after 100 cycles, while the capacity retention rate of the electrolyte system without FEC and the ordinary commercial electrolyte system is only 77 and 68%, respectively. Besides, the rate performance of the battery with the addition of FEC also shows excellent performance, however, this kind of advantage is not obvious under the conditon of large rate. In addition, under the conditon of the synergistic effect between adiponitrile and fluoroethylene carbonate, the high-voltage electrolyte exhibits the good compatibility and lithium reversibility in the full cell with Li4Ti5O12 as the negative electrode.