Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 10 de 10
Filter
Add more filters










Publication year range
1.
J Agric Food Chem ; 2024 Aug 06.
Article in English | MEDLINE | ID: mdl-39105697

ABSTRACT

Gynostemma pentaphyllum has been used as an herbal tea, vegetable, and dietary supplement for hundreds of years in East Asia. The sweet variety, grown in large areas in Fujian Province, China, is an essential source of "Jiaogulan" herbal tea. However, its sweet components are unknown. To investigate the sweet constituents of Fujian "Jiaogulan" and discover new natural high-potency sweeteners, phytochemical and sensory evaluations were combined to obtain 15 saponins, of which 11 (1-11) were sweet-tasting, including 2 new ones with sweetness intensities 20-200 times higher than that of sucrose, and four (12-15) were bitter-tasting. Their structures were elucidated using spectroscopic methods (NMR, MS, IR, UV), hydrolysis, and comparison with literature data. The contents of the 15 saponins were quantitatively analyzed using UPLC-MS/MS in multiple reaction monitoring mode. The contents of 1 and 2 sweet-tasting gypenosides were 9.913 ± 1.735 and 35.852 ± 1.739 mg/kg, respectively. The content of the sweetest compound (6) was 124.969 ± 0.961 mg/kg. Additionally, compound 4 was the most abundant sweet component (422.530 ± 3.702 mg/kg). Furthermore, molecular docking results suggested interactions of sweet saponins with sweet taste receptors. In general, this study revealed the material basis of the Fujian "Jiaogulan" taste.

2.
J Adhes Dent ; 25(1): 23-30, 2023 Jan 23.
Article in English | MEDLINE | ID: mdl-36689275

ABSTRACT

PURPOSE: To investigate the bond durability of composite cement to zirconia after treatment with a 15-methacryloyloxypentadecyl dihydrogen phosphate (15-MPDP)-containing adhesive and 2 commercially available adhesives. MATERIALS AND METHODS: Ninety zirconia bars were fabricated and bonded to prepolymerized resin composite cylinders with a composite cement after surface treatment for 20 s using the following adhesives: Adper Easy One (AEO, negative control), Single Bond Universal (SBU, positive control), and 10 wt% 15-MPDP powder mixed with Adper Easy One (15- MPDP). After storage in distilled water at 37°C for 24 h, the specimens were divided into 3 subgroups according to the aging treatment applied (n = 10): no aging treatment (0/TC), 10,000 thermocycles (1/TC), and 37,500 thermocycles (3/TC). Shear bond strength (SBS) was analyzed using two-way ANOVA (p < 0.05), and the fracture surfaces were examined under a dental microscope. RESULTS: Significant differences in the SBSs among the surface treatments and aging treatments were observed (both p < 0.001). The 15-MPDP and SBU groups showed significantly higher SBSs than the AEO group, whereas similar SBSs were found in the 15-MPDP and SBU groups. Significant reductions in the SBSs were found after 37,500 thermocycles (p < 0.001), although no significant difference between specimens aged with 10,000 thermocycles and non-aged specimens was observed. CONCLUSIONS: The 15-MPDP-containing dental adhesive exhibited bond durability comparable to that of a well-established 10-MDP-containing universal adhesive. Aging by 10,000 thermocycles may be insufficient to disrupt the bond of composite cement to zirconia.


Subject(s)
Dental Bonding , Phosphates , Surface Properties , Resin Cements , Zirconium , Glass Ionomer Cements , Shear Strength , Materials Testing
3.
Medicine (Baltimore) ; 101(31): e30026, 2022 Aug 05.
Article in English | MEDLINE | ID: mdl-35945710

ABSTRACT

To investigate the normal diameter of the abdominal aorta and common iliac arteries of the middle-aged and elderly people in China and the relationship of the diameters with age, sex, height, weight, body mass index (BMI), and body surface area (BSA). This retrospective study enrolled 625 patients including 380 males and 245 females aged 60.00 years (interquartile range 13.00 years). All clinical data and the diameters of the abdominal aorta and common iliac arteries were analyzed. The diameter of the abdominal aorta was 21.49 ± 2.49 mm at the proximal, 16.94 (interquartile range 2.39) mm at the middle, and 15.65 (interquartile range 2.90) mm at the distal segment. The diameter of the common iliac artery was 10.76 (interquartile range 1.99) mm at the right proximal, 10.41 (interquartile range 2.05) mm at the left proximal, 10.74 (interquartile range 2.25) mm at the right distal, and 10.67 (interquartile range 2.22) mm at the left distal segment. The height, weight, BSA, BMI, diameters of the proximal, middle and distal abdominal aorta as well as the proximal and distal left and right common iliac arteries were significantly higher in males than those in females (P < .001). Height, weight, BSA, and BMI were significantly (P < .001) positively correlated with the diameter of the abdominal aorta and common iliac artery at the proximal, middle, and distal segments. The middle and distal diameters of the abdominal aorta were significantly higher in males than those in females (P < .05). The diameter of the abdominal aorta at the proximal, middle, and distal segment as well as the diameter of the left and right common iliac artery at the distal segment were significantly (P < .05) increased with age. The normal values of the diameter of the abdominal aorta and common iliac arteries are suggested for the middle-aged and elderly Chinese people for clinical reference. The diameters are gender related and significantly positively correlated with BSA, height, weight, and BMI, which is beneficial for the diagnosis and treatment planning of relevant vascular diseases.


Subject(s)
Aorta, Abdominal , Iliac Artery , Aged , Aorta, Abdominal/diagnostic imaging , Body Surface Area , China , Female , Humans , Iliac Artery/diagnostic imaging , Male , Middle Aged , Retrospective Studies
4.
Eur Radiol ; 31(4): 2332-2339, 2021 Apr.
Article in English | MEDLINE | ID: mdl-33000304

ABSTRACT

OBJECTIVE: To analyze missed rib fractures and proper time for evaluation on CT at different ages and to determine factors that favor missed fractures. METHODS: One hundred patients with rib fractures who underwent CT were classified into three groups according to their age: young, middle-aged, and elderly. CT was performed within 1 to 6 weeks after trauma. The imaging features and temporal changes of rib fractures were analyzed. RESULTS: At the first CT during the initial week, 638 ribs were detected with one or several fractures, overall 838 fractures were confirmed, and 6 were suspected. In the next 2-6 weeks, 47 occult rib fractures were additionally detected. The number of additionally diagnosed fractures was the highest in respectively the 3rd week among younger, 4th week in the middle-aged, and 6th week in the elderly groups. The detection of occult rib fractures was significantly delayed in the middle-aged and elderly groups compared with the young group (p < 0.05). The time to form bony callus was also significantly (p < 0.05) delayed with age, with significantly (p < 0.05) more time needed to form bony callus in the middle-aged (23.8 ± 4.5 days) and elderly (28.48 ± 5.1 days) groups than in the young group (18.0 ± 2.2 days). CONCLUSIONS: Most rib fractures can be detected within the first week after trauma. Detection of occult rib fractures will be delayed with increase of age, and repeated CT scanning should be appropriately postponed in patients at different ages. Trabecula, inner and outer plates, costal angle, and cartilage are the primary locations for occult and subtle fractures which should be carefully evaluated. KEY POINTS: • More rib fractures can be detected on repeated CT scans, especially for subtle and occult rib fractures. • Detection of all rib fractures helps relieve the patient's concerns and determine the degree of personal injury for appropriate evaluation.


Subject(s)
Rib Fractures , Wounds, Nonpenetrating , Aged , Humans , Middle Aged , Retrospective Studies , Rib Fractures/diagnostic imaging , Ribs , Tomography, X-Ray Computed
5.
Int J Nanomedicine ; 11: 2359-67, 2016.
Article in English | MEDLINE | ID: mdl-27313453

ABSTRACT

The objective of this study was to develop an amiodarone hydrochloride (ADHC)-loaded liposome (ADHC-L) formulation and investigate its potential for cardiomyocyte targeting after cardiac radiofrequency ablation (CA) in vivo. The ADHC-L was prepared by thin-film method combined with ultrasonication and extrusion. The preparation process was optimized by Box-Behnken design with encapsulation efficiency as the main evaluation index. The optimum formulation was quantitatively obtained with a diameter of 99.9±0.4 nm, a zeta potential of 35.1±10.9 mV, and an encapsulation efficiency of 99.5%±13.3%. Transmission electron microscopy showed that the liposomes were spherical particles with integrated bilayers and well dispersed with high colloidal stability. Pharmacokinetic studies were investigated in rats after intravenous administration, which revealed that compared with free ADHC treatment, ADHC-L treatment showed a 5.1-fold increase in the area under the plasma drug concentration-time curve over a period of 24 hours (AUC0-24 h) and an 8.5-fold increase in mean residence time, suggesting that ADHC-L could facilitate drug release in a more stable and sustained manner while increasing the circulation time of ADHC, especially in the blood. Biodistribution studies of ADHC-L demonstrated that ADHC concentration in the heart was 4.1 times higher after ADHC-L treatment in CA rat model compared with ADHC-L sham-operated treatment at 20 minutes postinjection. Fluorescence imaging studies further proved that the heart-targeting ability of ADHC-L was mainly due to the CA in rats. These results strongly support that ADHC-L could be exploited as a potential heart-targeting drug delivery system with enhanced bioavailability and reduced side effects for arrhythmia treatment after CA.


Subject(s)
Amiodarone/administration & dosage , Amiodarone/chemistry , Catheter Ablation/methods , Liposomes/administration & dosage , Myocytes, Cardiac/drug effects , Amiodarone/pharmacokinetics , Animals , Anti-Arrhythmia Agents/administration & dosage , Anti-Arrhythmia Agents/chemistry , Anti-Arrhythmia Agents/pharmacokinetics , Biological Availability , Drug Delivery Systems/methods , Drug Liberation , Liposomes/chemistry , Male , Microscopy, Electron, Transmission , Nanoparticles/administration & dosage , Nanoparticles/chemistry , Optical Imaging , Particle Size , Rats, Sprague-Dawley , Tissue Distribution
6.
Chinese Journal of Burns ; (6): 46-48, 2009.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-257446

ABSTRACT

<p><b>OBJECTIVE</b>To observe changes of learning and memory ability (LMA) in burn rats with depression, and study the relationship between LMA and expression of hippocampal NMDA.</p><p><b>METHODS</b>According to simple random method, 46 Wistar rats were divided into burn group (B, with 30% TBSA deep partial-thickness burn, n = 10), depression group (D, with moderate stress stimulation in chronic and unpredictable, n = 12), B + D group (with the same stress stimulation inflicted to B group after burn, n = 12), healthy control group ( HC, without treatment, n = 12). Changes in escape latency was examined in water maze test. Expression of hippocampal NMDA in CA1, CA2 regions and dentate gyrus were observed with immunohistochemistry.</p><p><b>RESULTS</b>Compared with that of HC group (22 +/- 20 s), water maze escape latency in B, D, B + D groups on 2 day after training prolonged (38 +/- 31, 41 +/- 36, 42 +/- 33 s, respectively, P < 0.05 or P < 0.01). Water maze escape latency in B + D group on 4th day after training was longer than that of other groups (P < 0.01). There was no obvious difference in positive expression of NMDA in CA1, CA2 regions among groups (P > 0.05). The positive count of NMDA in dentate gyrus in D group (198 +/- 14) and B + D group (191 +/- 6) were lower than that of HC group (224 +/- 23, P < 0.05 or P < 0.01), but there was no obvious difference between HC group and B group (219 +/- 25, P > 0.05).</p><p><b>CONCLUSIONS</b>Burn complicated with depression can reduce LMA, which may be due to a decrease in NMDA in dentate gyrus.</p>


Subject(s)
Animals , Rats , Burns , Metabolism , Psychology , Depression , Hippocampus , Metabolism , Maze Learning , Physiology , Memory , N-Methylaspartate , Metabolism , Rats, Wistar
7.
Chinese Journal of Epidemiology ; (12): 868-871, 2007.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-322907

ABSTRACT

<p><b>OBJECTIVE</b>To explore the status and influencing factors on anxiety sensitivity among middle school students in Chongqing.</p><p><b>METHODS</b>58 classes from 12 schools were randomly selected in four administrative districts of Chongqing city. A total number of 2700 students was included for final analysis including 48.5% from junior high school and 51.5% from senior high school students with 49.2% boys and 50.8% girls. The Chinese version of the Anxiety Sensitivity Index-Revision, Adolescent Self-Rating Life Events Check List (ASLEC) and State-Trait Anxiety Inventory (STAI) were used.</p><p><b>RESULTS</b>(1) There was no significant difference between grade groups (P = 0.49). (2) The level of girl's anxiety sensitivity was always higher than boy's (P < 0.001). (3) Data from multiple linear regression showed that the influential factors to the degree of anxiety sensitivity were: state of anxiety, trait anxiety, life events, sex, stress from learning, etc (standard coefficients of regression were 0.258, 0.163, 0.112, 0.093, 0.124, -0.096, 0.096).</p><p><b>CONCLUSION</b>The major influential factors of anxiety sensitivity would include: sex, stress from learning, life events, interpersonal relationship, state of anxiety and trait anxiety.</p>


Subject(s)
Adolescent , Child , Female , Humans , Male , Anxiety , Psychology , Linear Models , Psychometrics , Stress, Psychological , Students , Psychology
8.
Chinese Journal of Burns ; (6): 288-290, 2006.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-331578

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the self-esteem level and social adaptation ability of hospitalized burn patients in our burn ward.</p><p><b>METHODS</b>One hundred and twenty hospitalized burn patients in our burn ward were enrolled in the study and evaluated according to their sex, severity of burn injury and education level. Their self-esteem level and social adaptation ability were scored with the Felling of Inadequacy Scale and Abbreviated Burn Specific Health Scale.</p><p><b>RESULTS</b>The general score of self-esteem of the patients with mild burns( 183+/-23) was obviously lower than that with moderate and severe burns (167+/-21 and 154 +/-24) , ( P <0.01). The self-esteem level of burn patients was different in different sex and education level. Among the self-esteem scores, male burn patients presented evidently higher scores of self evaluation, social ability, appearance, as well as the general score than those in the female ( P < 0.05). Moreover, the self evaluation score and study ability was higher in those with higher education level than those with lower education. Furthermore, the score of social adaptation ability was higher in the patients with mild burns than that in patients with moderate and severe burns ( P < 0. 01). The social adaptation ability and psychological function were much higher in male patients than those in female patients, but the former were weaker than the latter in regard to the body function. The psychological function, social relationship and general condition of the patients with lower education were better than those with higher education ( P <0. 05 ).</p><p><b>CONCLUSION</b>There existed difference in the self-esteem and social adaptation ability in different burn patients during different periods.</p>


Subject(s)
Adolescent , Adult , Female , Humans , Male , Middle Aged , Burns , Psychology , Inpatients , Psychology , Self Concept , Social Adjustment
9.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-735544

ABSTRACT

Objective To investigate the validity of the Chinese version of the Symptom Check-List-90 (the SCL-90) in Chinese population. Methods A total of 624 normal person and 268 out patients were examined with the SCL-90. Results (1)The internal consistency of the original subscales was found to be good and Cronbach's coefficient alpha ranged from 0.78 to 0.90; (2)Discrimination function analysis based on the 9 original subscales showed that the power of the SCL-90 in discriminating the patients and the community was good with a corrective rate of 80.6%; (3)Factor analysis on the items of the questionnaire yielded a very strong un-rotated first factor which could represent other factors; (4)High inter-correlations were found between the 9 original subscales of the SCL-90 with a correlation coefficient ranging from 0.39 to 0.79. Conclusion (1) The SCL-90 is acceptable as a research setting and psychiatric screening inventory; (2) The SCL-90 does not have potential as an inventory to distinguish different diagnostic groups; (3) The SCL-90 is not a multidimensional inventory.

10.
Article in Chinese | WPRIM (Western Pacific) | ID: wpr-737012

ABSTRACT

Objective To investigate the validity of the Chinese version of the Symptom Check-List-90 (the SCL-90) in Chinese population. Methods A total of 624 normal person and 268 out patients were examined with the SCL-90. Results (1)The internal consistency of the original subscales was found to be good and Cronbach's coefficient alpha ranged from 0.78 to 0.90; (2)Discrimination function analysis based on the 9 original subscales showed that the power of the SCL-90 in discriminating the patients and the community was good with a corrective rate of 80.6%; (3)Factor analysis on the items of the questionnaire yielded a very strong un-rotated first factor which could represent other factors; (4)High inter-correlations were found between the 9 original subscales of the SCL-90 with a correlation coefficient ranging from 0.39 to 0.79. Conclusion (1) The SCL-90 is acceptable as a research setting and psychiatric screening inventory; (2) The SCL-90 does not have potential as an inventory to distinguish different diagnostic groups; (3) The SCL-90 is not a multidimensional inventory.

SELECTION OF CITATIONS
SEARCH DETAIL
...