ABSTRACT
Aquaporin 1 (AQP1) is one of the water channels involved in water transfer and reabsorption. The results of a reverse transcription-polymerase chain reaction (RT-PCR) indicated that the level of AQP1 mRNA was increased in the testis and vas deferens, but decreased in the epididymis from the early postnatal period through puberty to adulthood. The results of Western blot analyses largely matched the RT-PCR findings for AQP1. AQP1 was immunohistochemically localized on the plasma membrane in the epithelia of rete testis and vas deferens at postnatal day 70, although it was hardly detectable at postnatal day 15. Nevertheless, AQP1 was expressed in the epithelia of the efferent duct and the initial segment of caput epididymis at postnatal day 15 and persisted into adulthood. The results taken together suggest that the expression and localization of AQP1 in mouse testis, epididymis and vas deferens are a stage-, tissue- and region-specific pattern and provide obvious clues that a possible relationship exists between the functional application of AQP1 for water reabsorption, and male reproduction and maturation.
Subject(s)
Aquaporin 1/genetics , Aquaporin 1/metabolism , Epididymis/metabolism , Gene Expression Regulation, Developmental , Testis/metabolism , Vas Deferens/metabolism , Animals , Blotting, Western , Immunohistochemistry , Male , Mice , Mice, Inbred ICR , Reverse Transcriptase Polymerase Chain Reaction , Time FactorsABSTRACT
Objective To observe the distribution and the effect of the quantum dots(QDs) on mouse abdominal cavity macrophages.Methods The QDs were co-cultured with mouse abdominal cavity macrophages in vitro.The differentiation and effect of the QDs on macrophage ultrastructures were observed under electronic microscope. Results The QDs were enveloped with unit membrane and internalized in the cytoplasm of the macrophage under transmission electron microscope.And it formed vacuolelike structures in the macrophage.There were many lamellar processes on the surface of the macrophage under scanning electron microscope.Conclusion The QDs can promote macrophage activation,and make its surface projection increased.The QDs were internalized by the macrophage,distributed in the cytoplasm,and formed vacuolelike structures enveloped with unit membrane.
