Bioelectrochemical regulation accelerates biomethane production from waste activated sludge: Focusing on operational performance and microbial community.

Bioelectrochemical regulation represents a newly emerging strategy to enhance anaerobic digestion (AD) of biowastes. Herein, a novel microbial electrolysis cell (MEC) system, equipped with a pair of carbon brush anode and hybrid Ti/RuO2-graphite felt cathode, was developed to explore the role of bioelectrochemical regulation in the proliferation/enrichment of functional microbes and methanation of waste activated sludge. The methane production was significantly improved by applying bioelectrochemical regulation. The maximum methane yield was 16.4 mL/L-reactor at the applied external voltage 1.2 V and solids retention time 15 d, 8.6-time higher than that of a single AD. Further analysis demonstrated that bioelectrochemical regulation selectively enriched electroactive fermentative partners and methanogens (especially Thermincola, Methanobacterium) in the MEC-AD system and built up a robust syntrophic interaction. This drove the decomposition of complex organics and concurrent bioelectroreduction of CO2 in biogas and subsequently enhanced methane generation. Besides, bioelectrochemical simulation attenuated N2O emissions and enhanced the dewaterability of digested sludge.

[Enhanced Membrane Anti-fouling Ability of Anaerobic Membrane Bioreactor Equipped with Bio-electrochemical System Using Nano-zero-valent Iron and Its Regulation Mechanism].

Membrane fouling is the biggest challenge of membrane bioreactor industrialization. In this study, a bio-electrochemical system (BES)-anaerobic membrane bioreactor (AnMBR) system was constructed, and the effect of nano-zero-valent iron (nZVI) on membrane anti-fouling ability and methane production was investigated. The results showed that the BES-AnMBR system was stable and the chemical oxygen demand (COD) removal rate was maintained at approximately 95%. The optimum condition was observed to be nZVI 0.1 g·g-1(VS). Under this condition, transmembrane pressure (TMP) was reduced by 28.1%, the membrane flux had a slight improvement, and methane production was up to 81.3 mL·g-1(CODremoved). This was 12.1% higher than that of the control. In addition, a further analysis of extracellular polymeric substances (EPS) fraction and membrane resistance showed that nZVI enhanced EPS decomposition, promoted the formation of an iron-rich layer of inorganic and organic matters on the membrane surface, and changed the distribution of organics and inorganics, thereby significantly alleviating membrane fouling. This study will enrich basic theory of conventional AnMBR and provide a new solution for efficient sludge treatment and resource utilization.

Electrically regulating co-fermentation of sewage sludge and food waste towards promoting biomethane production and mass reduction.

Microbial electrolysis cell (MEC) was integrated into conventional anaerobic digestion (AD) system (i.e. MEC-AD) to electrochemically regulate the co-fermentation of food waste (FW) and sewage sludge (SS). Two anaerobic systems (i.e. MEC-AD, and single AD) were operated in parallel to explore the potential stimulation of electrical regulation in metabolic behaviors of FW and SS and subsequent biomethane production. The highest accumulative methane yield was achieved at an applied voltage of 0.4 V and the FW and SS ratio of 0.2:0.8, increasing by 2.8-fold than those in AD. The combined MEC-AD system mitigated N2O emission and considerably improved ammonia removal and the dewaterability of digestate, in contrast to AD. Scanning electron microscope (SEM) visualized the presence of a large number of rod-like and cocci-like electroactive microbes on the electrode surface. Electrical regulation stimulated the self-growth and proliferation of typical Methanobacterium and Methanosaeta, accordingly contributing to biomethane production greatly.