ABSTRACT
Objective To explore whether neural stern cells in vitro express the chemokine receptor CX3CR1. Methods The neural stem cells were isolated from the hippocampi of neonatal rats and cultured in a serum-flee medium. Using immunofluorescence, the neural stem cells and their differentiation into neural and glial cell types were identified; then, the immunofluorescence and RT-PCR were employed to detect whether the neural stem cells in vitro expressed chemokine receptor CX3CR1. Results The floating neural spheres, isolated from neonatal rat hippocampi, were nestin positive and could differentiate into cells to express characteristic antigens of neurons, astrocytes and oligodendrocytes, Immunofluorescence and RT-PCR showed that neural stem cells expressed chemokine receptor CX3CR1. Conclusion Neural stem cells in vitro can express chemokine receptor CX3CR1, which offers the theoretical evidence for further study of its migration.
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical effect of lateral lymph nodes dissection and autonomic nerve preservation in anterior resection for rectal cancer.</p><p><b>METHODS</b>One hundred and twenty-four patients with rectal cancer underwent anterior resection with lateral lymph nodes resection and autonomic nerve preservation. The patients were followed-up through post-operational questionnaire about the function of defecation, urination and sex after the operation. And post-operative survival was analyzed retrospectively.</p><p><b>RESULTS</b>Urinary catheters were removed in 112 cases (90.3%) in 3 days post operation, the mean time of indwelling catheter was (58.3 +/- 2.1) h. Nineteen patients experienced fecal incontinence, 12 cases of them recovered through release-training and one recovered spontaneously. Of the 98 questionnaire respondents, 61 cases (62.3%) could erect normally, and 56 cases (57.1%) had normal sexual function. The max-micturition-desire urine volume was (401.2 +/- 23.1) ml and the residual urine volume was (28.2 +/- 2.2) ml. Five year survival rate of all the patients was 61.2%.</p><p><b>CONCLUSIONS</b>Lateral lymph nodes dissection and autonomic nerve preservation in anterior resection for rectal cancer can decrease the post-operative dysfunction of defecation, urination and sex life and does not affect the survival.</p>
Subject(s)
Adult , Humans , Male , Middle Aged , Anal Canal , Autonomic Pathways , Follow-Up Studies , Lymph Node Excision , Methods , Pelvis , Postoperative Complications , Rectal Neoplasms , Mortality , General Surgery , Survival Analysis , Survival Rate , Treatment OutcomeABSTRACT
<p><b>OBJECTIVE</b>To investigate the clinical value of extended radical resection with nerve- preservation for rectal cancer.</p><p><b>METHOD</b>Ninety-eight patients with rectal cancer received extended radical resection with nerve- preservation in our hospital. The questionnaire were used to collect the data of the patients urination and sexual function. The survival was analyzed retrospectively.</p><p><b>RESULTS</b>62.3% (61/98) of the patients could erect normally and 57.1% (56/98) of the patients had normal sexual function. The average time of catheterization in 57 patients was 60 hours, the residual urine volume (RUV) was 28 ml and the max-micturition-desire urine volume was 400 ml. The 5-year survival rate of those who underwent extended radical resection with nerve-preservation was 61.2%.</p><p><b>CONCLUSION</b>Extended radical resection with nerve-preservation,which could decrease the incidences of post-operative urination and sexual dysfunction, and have not affect the survival, was the most optimal operation for rectal cancer.</p>