ABSTRACT
A simple and sensitive method for rapid detection of an antigen is offered. 0.025 ml of antiserum is placed on the slide, 0.025 ml of clarified extract containing the antigen added, and then 0.025 ml of intact 20.10(6) suspension of Kowan 1 Staphylococcus strain is added. When antigen-antibody complex is forming, staphylococcal cell aggregation starts. A staphylococcal reagent, manufactured by the Pasteur Research Institute of Epidemiology and Microbiology in Leningrad, may be employed, this making the method more available. The indication sensitivity is 0.2-1.25 mln cells per ml, this value depending on the bacterial species.
Subject(s)
Antigens, Bacterial/analysis , Humans , Time FactorsABSTRACT
Investigation of bacterial contamination in curds and sour cream, and experimental studies on survival rate of a number of pathogenic and opportunistic microorganisms in these products have permitted standardization of the products with respect to coliform bacteria. A permissible level has been determined for bacterial contamination of curds and sour cream.