ABSTRACT
Complying with overall requirements of the government and regulations on public health emergences, as well as the clinical features of diagnosis and treatment of dental illness, this paper refers to previous guidelines and studies on the infection prevention and control in dental diagnosis and treatment in China and foreign countries. Nanjing Stomatological Hospital has implemented the emergency management practices for the prevention and control of novel coronavirus pneumonia (NCP), mainly focusing on the implementation of prevention and control training programs for medical staffs and the infection control projects on the hospital environment. This study could provide reference for rapid response and emergency management for the prevention and control of NCP in the departments of stomatology.
ABSTRACT
OBJECTIVE@#: To investigate the effect of polysaccharides (LBPs) on TLR/NF-κB independent pathway and serum tumor necrosis factor (TNF-α) level in diabetic MyD88-knockout mice.@*METHODS@#: Diabetes was induced by feeding high-fat/high-sugar diet and injection of low-dose streptozotocin in MyD88-knockout mice. The diabetic mice were randomly divided into model group, positive control group and LBPs group. The expressions of TRAM, TRIF, TRAF6, RIP1 and TNF-α mRNA and proteins in mouse peritoneal macrophages were detected by real-time RT-PCR and Western blotting after LBPs treatment for 3 month. Serum TNF-α was determined with ELISA kit.@*RESULTS@#: Real time RT-PCR showed that compared with model group, the relative expressions of and mRNA in macrophages of LBPs group were significantly decreased and expression of was significantly increased (all 0.05).@*CONCLUSIONS@#: LBPs may not inhibit serum TNF-α level through TLR/NF-κB independent pathway.
Subject(s)
Animals , Mice , Diabetes Mellitus, Experimental , Drugs, Chinese Herbal , Pharmacology , Gene Expression Regulation , Macrophages, Peritoneal , Mice, Knockout , Myeloid Differentiation Factor 88 , Genetics , NF-kappa B , Genetics , Random Allocation , Signal Transduction , Tumor Necrosis Factor-alpha , Blood , MetabolismABSTRACT
OBJECTIVE To ensure clinical application of standard procedure in the management of dental handpieces collection and sterilization.METHODS To establish the center of sterilization and supply for dental handpieces;to equip with the device for depuration and sterilization;to standardize the procedure for depuration,retrieval,pre-disposal treatment,cleaning,drying,lubrication,envelopment and sterilization.RESULTS The management of collection and sterilization for dental handpieces ensured the clinical application of standard procedure,avoided the exogenous infection and guaranteed the patients safety.CONCLUSIONS The clinical management of collection and sterilization for dental handpieces is an effective method.
