ABSTRACT
Transgenic Nicotiana tabacum L. cv. SR1 plants, characterized by an increase in the level of dsRNA-specific hydrolytic activity after induction by wounding, were obtained. The Solanum lycopersicum anionic peroxidase gene promoter (new for plant genetic engineering) was for the first time used for the induced expression of the target Serratia marcescens RNase III gene. Upon infection with the tobacco mosaic virus (TMV), the transgenic plants of the obtained lines did not differ significantly from the control group in the level of TMV capsid protein accumulation. In general, no delay in the development of the infection symptoms was observed in transgenic plants as compared with the control group. The obtained transgenic plants represent a new model for the study of the biological role of endoribonucleases from the RNase III family, including in molecular mechanisms of resistance to pathogens.
Subject(s)
Bacterial Proteins , Genes, Bacterial , Nicotiana , Plants, Genetically Modified , Ribonuclease III , Serratia marcescens/genetics , Bacterial Proteins/biosynthesis , Bacterial Proteins/genetics , Disease Resistance , Plants, Genetically Modified/enzymology , Plants, Genetically Modified/genetics , Ribonuclease III/biosynthesis , Ribonuclease III/genetics , Serratia marcescens/enzymology , Nicotiana/enzymology , Nicotiana/geneticsABSTRACT
The method of virus-induced gene silencing (VIGS) based on posttranscriptional gene silencing (PTGS) is a promising new method for the study of plant gene functions. In the current review we analyzed works on the development and improvement of this method, including the creation of new viral constructions for different plant species, the search for new reporter genes for the control of VIGS efficiency, and the development of new efficient methods of infection.
Subject(s)
Gene Silencing , Genetic Engineering , Plant Viruses/genetics , Plants/genetics , Plants/virology , Plant Viruses/metabolism , Plants/metabolismABSTRACT
A prototype of oligonucleotide microarray for detection of Lassa, Junin, Machupo, Guanarito viruses (Arenaviridae family), Ebola and Marburg viruses (Filoviridae family) was presented. An original approach founded on virus proteins (nucleocapsid protein for Junin, Guanarito, Machupo viruses and RNA-dependent RNA-polymerase for Lassa, Ebola and Marburg viruses) amino acid sequences analysis with subsequent transform of revealed unique peptides into due sets of oligonucleotides was used to design probes for hybridization and primers.
