ABSTRACT
Objective To investigate the synergic ratio of sorafenib (SO) and sulforaphane (SF) against the hepatocellu‐lar carcinoma cell line HepG2 in vitro .Methods The synergic effect of SO combined with SF against HepG2 cells was deter‐mined by the CCK8 assay (the synergic effect was determined by combination index (CI) value:CI>1 .1 ,antagonistic;0 .9<CI<1 .1 ,additive;CI<0 .9 ,synergic) .The effectiveness of the synergic effect of the synergic ratio was confirmed by the An‐nexinV‐FITC apoptosis experiment and colony formation assay .Results The CCK‐8 assay showed that SO combined with SF at the molar rate of 1∶1 ,1∶10 and 1∶20 exhibited strong antagonism (CI>1 .1) ,whereas 20∶1 ,5∶1 and 1∶5 ratio resul‐ted in synergistic activity (CI<0 .9) .Furthermore ,10∶1 ratio acted as an additive effective (0 .9<CI<1 .1) .The number of colony formation (14 .66 2 .08) of the group treated with SO combined with SF at 5∶1 synergistic ratio was significantly lower than that of the group treated with after SO combined with SF at 1∶1 antagonistic ratio (31 .33 4 .16) (P<0.01) .The early and late apoptosis rate of HepG2 cells (21 .71 ± 6 .06) of the group treated with SO combined with SF at 5∶1 synergistic ratio was significantly higher than that of the group treated with SO combined with SF at 1∶1 antagonistic ratio(6 .64 ± 0 .311)(P<0.01) .Conclusion SO combined with SF at the molar rate of 5∶1 can synergistically inhibit the growth of hepatocellular car‐cinoma cell line HepG2 in v itro .
ABSTRACT
Objective To prepare and characterize salinomycin sodium‐loaded nano liposomes(SLN) .Methods The nano liposomes were prepared by a thin‐film dispersion method .The formula of SLN was optimized by regulating the cholesterol ratio of the nano liposomes ,using the encapsulation efficacy (EE) of SLN as the primary outcome measure .Results Transmission e‐lectron microscope (TEM) showed that SLN was round and had a good dispersion .Dynamic laser scatter (DLS) showed that SLN was of a desired size of 99 nm ,and zeta potential of -33 .5 mV .EE of SLN was 85 .7% and drug loading of 6 .7% .Ac‐cording to the formulation of nano liposomes ,the concentration of salinomycin sodium in water was greatly improved by 15 folds .Additionally ,the nano liposomes were observed to exhibit sustained release characteristics .Conclusion Salinomycin sodi‐um‐loaded nanoliposomes of a desired size of about 100 nm were obtained ,which were well dispersion ,and high EE and drug loading .Solid pharmaceutics foundation for the activity examination of SLN was provided in this research .
