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1.
Stomatologiia (Mosk) ; 93(5): 9-12, 2014.
Article in Russian | MEDLINE | ID: mdl-25588332

ABSTRACT

The aim of the study was to reveal the mastication forces effect on the microstructure of mandible bone tissue of mini-pigs by Fouirier harmonic imaging analysis of bone sections images of back scattered electrons and assessment of calcium and phosphorous distribution maps obtained by roentgenofluorescence technique. The results showed that by higher functional loads not only the total content of mineral elements in the bone matrix increased but also the of the low-frequency harmonics in the image spectrum indicating structural heterogeneity decrease in bone mineralization.


Subject(s)
Alveolar Process/ultrastructure , Bone Matrix/ultrastructure , Calcification, Physiologic , Image Processing, Computer-Assisted/methods , Mandible/ultrastructure , Mastication , Alveolar Process/diagnostic imaging , Alveolar Process/metabolism , Animals , Bone Matrix/diagnostic imaging , Bone Matrix/metabolism , Mandible/diagnostic imaging , Mandible/metabolism , Minerals/metabolism , Radiography , Spectrometry, X-Ray Emission , Swine , Swine, Miniature
2.
Stomatologiia (Mosk) ; 92(4): 9-13, 2013.
Article in Russian | MEDLINE | ID: mdl-23994847

ABSTRACT

The mandible alveolar bone tissue of mini-pig was evaluated by means of radiospectral analysis. Fourier analysis of mineral content distribution in bone slices showed that loss of masticatory load leads to focal demineralization. With time the demineralization foci increased in amount, not in size.


Subject(s)
Absorptiometry, Photon/methods , Alveolar Process/diagnostic imaging , Fourier Analysis , Tooth Demineralization/diagnostic imaging , Animals , Bone Density , Dental Enamel/diagnostic imaging , Dental Enamel/pathology , Dentin/diagnostic imaging , Dentin/pathology , Swine , Swine, Miniature , Tooth Demineralization/pathology
3.
Stomatologiia (Mosk) ; 86(2): 9-11, 2007.
Article in Russian | MEDLINE | ID: mdl-17828061

ABSTRACT

The destruction of human serum albumin was studied under the influence of the preparations used for chemical-mechanic removal of carious dentine (GK-101 and Caricleans, gel #2) and model systems containing sodium hypochlorite and amino compounds. Control contained hydroxide and sodium chloride but not hypochlorite. The degree of albumin destruction was determined by the amount of low molecular weight fragments precipitated by phosphotungstic acid but not by trichloracetic acid. From the results of the study the conclusion was made that in order to reduce the injuring action of hypochlorite upon dental tissues and soft tissues of oral cavity it is more preferable to use systems containing the addition of neutral and basic amino compounds.


Subject(s)
Chloramines/chemistry , Dental Caries/drug therapy , Dentin , Glycine/analogs & derivatives , Amines/chemistry , Chloramines/therapeutic use , Glycine/chemistry , Glycine/therapeutic use , Humans , Hydroxides/chemistry , Hypochlorous Acid/chemistry , Serum Albumin/chemistry , Sodium Chloride/chemistry
4.
Stomatologiia (Mosk) ; 86(3): 17-20, 2007.
Article in Russian | MEDLINE | ID: mdl-17690638

ABSTRACT

Study for determination of human individual sensitivity to fluoride was performed on 140 patients of CRIS clinics of the age from 20 to 65 years. It was established that even in cases of F-concentrations analogous to high physiological F-concentrations in blood serum (0.19 ppm) in 11.4% of the examined patients the signs of higher F-sensitivity were observed. Human individual sensitivity to fluoride was dependant upon the severity of patient's status. The results received can be used as a base for tactics development of timely and adequate correction of F-dosage.


Subject(s)
Cariostatic Agents/administration & dosage , Drug Evaluation/methods , Drug Tolerance , Fluorides/administration & dosage , Adult , Aged , Cariostatic Agents/analysis , Female , Fluorides/blood , Humans , Male , Middle Aged
5.
Stomatologiia (Mosk) ; 81(5): 11-4, 2002.
Article in Russian | MEDLINE | ID: mdl-12532880

ABSTRACT

Differences in the levels of tissue respiration in the rat skin flaps incubated in perfluothane (PF) and normal saline (NS) are regarded as an evidence of local PF effect on tissue substrate. The complex of metabolic changes in the skin fragments under the effect of PF in comparison with skin fragments incubated in NS indicate a protective effect of PF on tissue substrata. Decreased content of malonic dialdehyde and a slower accumulation of tissue liquid in skin flaps incubated in PF, in comparison with the fragments incubated in NS, is worthy of note. It indicates that incubation in PF (in comparison with incubation in NS) is less harmful for cell membranes and induces lesser disorders in water-salt metabolism and edema which are usually associated with tissue lesions developing under conditions of trophic deficiency.


Subject(s)
Blood Substitutes/pharmacology , Fluorocarbons/pharmacology , Skin/drug effects , Skin/metabolism , Surgical Flaps , Animals , Cell Respiration/drug effects , Cell Respiration/physiology , Cytoprotection/drug effects , Cytoprotection/physiology , Glycolysis/drug effects , Lipid Peroxidation/drug effects , Male , Rats , Rats, Wistar , Surgical Flaps/physiology , Tissue Preservation/methods
6.
Stomatologiia (Mosk) ; 78(5): 12-5, 1999.
Article in Russian | MEDLINE | ID: mdl-10533385

ABSTRACT

Relationship between alkaline phosphatase immobilized on apatite and remineralization processes has been studied. The enzyme notably accelerated the remineralization. The course of enzymatic remineralization depended on the source of phosphorus (inorganic phosphorus or beta-glycerophosphate). Calcium incorporation in remineralizing surface was the most rapid in the presence of beta-glycerophosphate. This may be explained by a higher rate of formation of apatite under the effect of immobilized alkaline phosphatase, than of brushite or vitlokite.


Subject(s)
Alkaline Phosphatase/pharmacology , Enzymes, Immobilized/pharmacology , Saliva/enzymology , Tooth Calcification/drug effects , Alkaline Phosphatase/antagonists & inhibitors , Apatites , Dental Enamel/drug effects , Enzyme Inhibitors/pharmacology , Enzymes, Immobilized/antagonists & inhibitors , Humans , In Vitro Techniques , Levamisole/pharmacology , Solutions , Tooth Remineralization/methods
7.
Stomatologiia (Mosk) ; 76(6): 9-12, 1997.
Article in Russian | MEDLINE | ID: mdl-9471822

ABSTRACT

The kinetic method revealed that by the rate of adsorption on apatite, serum alkaline phosphatase (AlP) is homologous and salivary AlP consists of two fractions: "slow" with the constant of adsorption rate approximating that of serum AlP and "fast" with 5-6 times greater constant. A mechanism of phosphatase immobilization on apatite by two-stage sequential reaction is proposed. The constants of rates of both stages for the serum phosphatase and fast salivary fraction are determined. Difference of the products of both stages by the Michaelis constant (KM) is demonstrated for the fast fraction. The KM of the second-stage immobilization product is close to that of AlP immobilized on dental enamel, which confirms the hypothesis about their identity. In contrast to AlP, both serum and salivary alpha-amylase react with apatite at the same rate and, probably, by the same mechanism as the bulk of salivary and serum protein.


Subject(s)
Alkaline Phosphatase/metabolism , Dental Enamel/enzymology , Enzymes, Immobilized/metabolism , alpha-Amylases/metabolism , Adsorption , Durapatite , Humans , Isoenzymes/metabolism , Kinetics , Particle Size , Saliva/enzymology
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